1.Clinical study on placental abruption.
Wan Suk CHO ; Geon O KIM ; Chang Yeon KIM ; Won Shik SHIN ; Sang Dae KANG ; Seung Bo PARK
Korean Journal of Obstetrics and Gynecology 1993;36(7):2304-2312
No abstract available.
Abruptio Placentae*
;
Female
;
Pregnancy
2.Clinical study on placental abruption.
Wan Suk CHO ; Geon O KIM ; Chang Yeon KIM ; Won Shik SHIN ; Sang Dae KANG ; Seung Bo PARK
Korean Journal of Obstetrics and Gynecology 1993;36(7):2304-2312
No abstract available.
Abruptio Placentae*
;
Female
;
Pregnancy
3.Isolation and characterization of fowl adenovirus serotype 4 from chickens with hydropericardium syndrome in Korea.
Hong Su PARK ; Il Soo LIM ; Sang Kyu KIM ; Toh Kyung KIM ; Sang Geon YEO
Korean Journal of Veterinary Research 2011;51(3):209-216
Four strains of fowl adenovirus (FAdV) were isolated from 4 flocks of broiler or layer chickens affected by hydropericardium syndrome in Korea. These FAdVs were classified as serotype 4 by restriction fragment length polymorphism patterns of hexon genes and whole genomes. The virus exhibited cytopathic effects consisting of rounding, ballooning and clustering in primary chicken embryo liver cell cultures. In transmission electron microscopy, virus particles in hexagonal shape aggregated exclusively in the nuclei of hepatocytes of the chickens as the typical appearances in adenovirus infections. Buoyant density of the virus in cesium chloride (CsCl) was 1.34 g/mL. The virus was stable to chloroform, ether, 50~70% ethanol, acidic condition at pH 3, 0.25% trypsin (1 : 250), heat at 50degrees C for 30 min, but labile to 100% ethanol, heat at 52~60degrees C for 30 min, 1 M MgCl2 at 50degrees C for 1 h, 1 : 2,000 formalin (37%). All of the physicochemical properties pertained to the characteristics of adenoviruses. Eight viral polypeptides were determined in CsCl-purified virus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Adenoviridae
;
Adenoviridae Infections
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Cell Culture Techniques
;
Cesium
;
Chickens
;
Chlorides
;
Chloroform
;
Electrophoresis
;
Embryonic Structures
;
Ethanol
;
Ether, Ethyl
;
Formaldehyde
;
Genome
;
Hepatocytes
;
Hot Temperature
;
Hydrogen-Ion Concentration
;
Korea
;
Liver
;
Magnesium Chloride
;
Microscopy, Electron, Transmission
;
Peptides
;
Polymorphism, Restriction Fragment Length
;
Sodium
;
Trypsin
;
Virion
;
Viruses
4.Utility of H-reflex in the Diagnosis Cervical Radiculopathy.
Jun LEE ; Gun Ju PARK ; Hyun Cheol DOO ; Sung Geon PARK ; Yun Seog JEONG ; Jung Sang HAH
Yeungnam University Journal of Medicine 1997;14(1):111-122
H-reflex is a kind of late respons which can be used for the proximal nerve conduction study. Also it is a useful and widely used nerve conduction technique es to look electrically at the monosynaptic reflex. Although recordable from all muscles theoretically, H-reflexes are most commonly recorded from the calf muscles following stimulation of the tibial nerve in the popliteal fossa. But in this study, We tried to establish the normal data and to evaluate the significance of the H-reflex study in cervical radiculopathy. H-reflexes were recorded from flexor carpi radialis (FCR) muscle, extensor carpi radialis (ECR) muscle, brachioradialis (BR) muscle, and abductor digiti minimi (ADM) muscle in 31 normal adults (62 cases) and 12 patients with cervical radiculopathy. The mean values of H-reflex latency in normal control group were 16.16+/- 1.65 msec in FCR; 15.99+/- 1.25 msec in ECR; 16.47+/- 1.59 msec in BR; 24.46+/- 1.42 msec in ADM. And the mean values of side to side difference of H-reflex latency were 0.47+/- 0.48 msec in FCR; 0.68+/- 0.72 msec in ECR; 0.63+/- 0.43 msec in BR; 22.31+/- 1.24 msec in ADM. Mean values of side to side differences of interlatency time were 0.49+/-0.47 msec in FCR; 0.73+/- 0.62 msec in ECR; 0.79+/- 0.71 msec in BR; 0.69+/- 0.44 msec in ADM. Also, there were no significant differences in H-reflex latency between right and left side. H-reflex tests in patient group with cervical radiculopathy revealed abnormal findings in 11 out of 12 patients. These results suggest that H-reflex in the upper extremity would be helpful in the diagnosis of the cervical radiculopathy.
Adult
;
Male
;
Female
;
Humans
6.The usefulness of the genetic markers at the low-density lipoprotein receptor gene locus for the genetic diagnosis of familial hypercholesterolemia.
Byoung Joo CHOI ; Hyun Young PARK ; Geon Young KIM ; Sang Min NM ; Seung Yun CHO ; Yang Soo JANG
Korean Journal of Medicine 2000;58(3):283-292
BACKGROUND: Familial hypercholesterolemia(FH) is an autosomal dominant metabolic disorder caused by the mutation in low density lipoprotein receptor(LDLR) gene. However, direct genetic diagnosis of LDLR gene mutation is not easily available because more than 300 mutations have been described in LDLR gene of FH patients. Therefore indirect genetic diagnosis using the genetic markers can be used to follow the inheritance of defective gene in FH families. The purpose of this study was to evaluate the usefulness of indirect genetic markers for detecting identical-by-descent LDLR gene abnormalities in FH families. METHODS: We examined the allele frequency, heterozygosity, polymorphism information content(PIC) of each genetic markers(D19S394, Taq I, Hinc II, Ava II, ATn, D19S221) in 94 unrelated healthy subjects. The genetic polymorphic haplotypes in 3 FH families were also determined. RESULTS: The heterozygosity and PIC values of RFLP's(Taq I, Hinc II, Ava II) were 0.51/0.344, 0.25/0.223, 0.28/0.233 and microsatellite markers(D19S394, ATn, D19S221) were 0.64/0.558, 0.56/0.455, 0.60/0.475. Hinc II and Ava II were significantly linked(|D|=0.72, p< 0.05). The cumulative PIC values of Taq I+Hinc II, Taq I+Hinc II+ATn, D19S394+ATn were 0.520, 0.814, 0.813, respectively. When applied in the FH pedigree, the genetic diagnosis using only one marker was not available in most cases. However, combination of two or more genetic markers could successfully discriminate the affected and unaffected members in FH families. Among the several combinations of the genetic markers, the combination of D19S394 and ATn was supposed to be the most effective and informative. Because one case of recombination was suspected in D19S221 allele, it was thought to be carefully used for genetic diagnosis of FH. CONCLUSION: We concluded that indirect genetic diagnosis using intragenic or extragenic genetic markers was useful for detecting identical-by-descent LDLR gene abnormalities in FH families and the most effective and informative combination of genetic marker seemed to be D19S394 and ATn.
Alleles
;
Diagnosis*
;
Gene Frequency
;
Genetic Markers*
;
Haplotypes
;
Humans
;
Hyperlipoproteinemia Type II*
;
Lipoproteins*
;
Microsatellite Repeats
;
Pedigree
;
Receptors, Lipoprotein*
;
Recombination, Genetic
;
Wills
7.A Case of Laryngeal Involvement as the Sole Presenting Manifestation of Systemic Lupus Erythematosus.
Young Ah LEE ; Sang Geon JEONG ; Seong Heon KIM ; Ga Yeon NAM ; Jae Hong PARK
The Journal of the Korean Rheumatism Association 2006;13(3):242-246
Laryngeal involvement is a rare complication of systemic lupus erythematosus (SLE) and can range from mild mucosal inflammation, vocal cord paralysis, and edema to necrotizing vasculitis with airway obstruction. Cricoarytenoid arthropathy is rare cause of severe airway obstruction in laryngeal involvement of SLE. In contrast to patients with rheumatoid arthritis, patients with SLE typically present with acute arthritis of cricoarytenoid joints and respond to corticosteroid therapy alone. We describe here a 13-year-old girl with SLE who presented with intermittent fever, swallowing difficulty, vocal cord paralysis and acute dyspnea required emergent tracheostomy as the sole manifestation of active SLE. She was diagnosed as having SLE based on the findings of positive anti-nuclear antibody and anti-ds DNA antibody with high titers, hypocomplementemia, leukocytopenia, and transient polyarthritis and proteinuria. No other clinical symptoms were found. Endoscopic findings showed edema of aryepiglottic fold, bilateral fixation of the vocal cords and edematous supraglottic structure with engorged vessels. Computered tomography scan of the neck showed diffuse soft tissue edema around the arytenoid and cricoid cartilages and enhancement of the aryepiglottic fold.
Adolescent
;
Airway Obstruction
;
Arthritis
;
Arthritis, Rheumatoid
;
Cricoid Cartilage
;
Deglutition
;
DNA
;
Dyspnea
;
Edema
;
Female
;
Fever
;
Humans
;
Inflammation
;
Joints
;
Larynx
;
Leukopenia
;
Lupus Erythematosus, Systemic*
;
Neck
;
Proteinuria
;
Tracheostomy
;
Vasculitis
;
Vocal Cord Paralysis
;
Vocal Cords
8.Clinical Features of Acute Pancreatitis in Children.
Jung Ho SEO ; Seong Heon KIM ; Sang Geon JEONG ; Jae Hong PARK
Korean Journal of Pediatric Gastroenterology and Nutrition 2010;13(1):58-65
PURPOSE: Since there are few studies involving acute pancreatitis in children, we reviewed our experience with this medical condition to describe the clinical features. METHODS: A retrospective analysis was conducted by reviewing the medical records of 41 patients with AP who were admitted to the Department of Pediatrics of Pusan National University Hospital between January 1996 and June 2007. RESULTS: Twenty males and 21 females (mean age, 8.7+/-4.5 years) were included. In 22 patients (53.7%), no definitive causes were found. The most common etiologies were choledochal cysts (22.0%). Necrotizing pancreatitis was diagnosed in 5 patients (12.2%), and recurrent acute pancreatitis in 4 patients (9.8%). CT findings included pancreatic swelling (43.9%), peripancreatic fluid collection (29.3%), ascites (24.4%), and peripancreatic fat necrosis (12.2%). Serum amylase and lipase levels at diagnosis were 535.3+/-553.2 and 766.2+/-723.6 U/L, respectively, and were normalized within 1 week in 22 and 14 patients, respectively. On the basis of the Balthazar scale, 2 patients were diagnosed with severe AP. In 4 patients (9.8%), a surgical procedure was indicated. Major complications included ascites (32.3%), sepsis (16.1%), and pseudocyst and renal impairments (12.9%). Two patients died from multi-organ failure. CONCLUSION: The etiologies of AP in children are varied. Most children have a single episode and a self-limited course. However, AP of childhood still carries significant morbidity and mortality. Early diagnosis, appropriate treatment according to disease severity, and management of complications are important.
Amylases
;
Ascites
;
Child
;
Choledochal Cyst
;
Early Diagnosis
;
Fat Necrosis
;
Female
;
Humans
;
Lipase
;
Male
;
Medical Records
;
Pancreatitis
;
Pediatrics
;
Retrospective Studies
;
Sepsis
9.Clinicostatistical study on orthognathic surgery in our department.
Sang Han LEE ; In Sook PARK ; Chang Hwan LEE ; Tae Geon KWON ; Man YUN ; Fangchen DENG
Journal of the Korean Association of Oral and Maxillofacial Surgeons 2005;31(3):255-259
We observed 469 cases (male 217 cases, female 252 cases) with dento-facial deformity for 10 years from Jan 1994 to Dec 2003. The ratio of male to female was 1:1.2 and the mean age was 23.3 years (male 24.0 years, female 22.6 years) ranged from 11 to 43 years.1) The most dominant group was related to mandibular prognathism (80.4%). A rate of 83.8% demonstrated mandibular protrusion according to Slavicek's classification (n=160), and 85.4% were classified as skeletal Class III type according to Sugawara's classification (n=151) Surgical method were divided into 355 cases of one jaw surgery (single method), 26 cases of one jaw surgery (combined method), and 77 cases of two jaw surgery. Sagittal split osteotomy were performed on 316 cases (69.0%). The average operation time and blood loss in SSRO were 4.1 +/- 2.2 hrs. and 138.8 +/- 222.6 ml (n=152)
Classification
;
Congenital Abnormalities
;
Female
;
Humans
;
Male
;
Orthognathic Surgery*
;
Osteotomy
;
Prognathism
10.The Effects of Glucocorticoid and alpha-Lipoic Acid on Ischemia-Reperfusion Injury.
Sang Soon LEE ; Yoon Jae CHUNG ; Byung Kyu SOHN ; Hyung Geon KIM ; Seong Ha PARK
Journal of the Korean Society of Plastic and Reconstructive Surgeons 2002;29(3):205-210
During revascularization after ischemia, oxygen free radicals and cytotoxic enzymes are released and they have a role in pathogenesis of ischemia-reperfusion injury. Glucocorticoid decreases oxygen free radical formation by inhibition of arachidonic acid metabolism, and alpha-lipoic acid scavenges nitric oxide(NO) with inhibition of hydroxy radical formation. Author investigated the role of glucocorticoid and alpha-lipoic acid to decrease ischemia reperfusion injury in 24 anesthetized rats (normal saline-injected, n= 8; dexamethasone-injected, n=8; alpha-lipoic acid-injected, n= 8), subjecting a soleus muscle to 4 hours of tourniquet ischemia followed by 2 hours of reperfusion, and evaluated the concentration of NO, tissue edema, and neutrophil count of rat skeletal muscle as a indicator of tissue damage by ischemia- reperfusion injury. We obtained the results that glucocorticoid and alpha-lipoic acid treatment decreased the increase of NO concentration, tissue edema, and neutrophil count significantly. These results support that pretreatment with glucocorticoid or alpha-lipoic acid has a beneficial effect on the preventive management of ischemia-reperfusion injury.
Animals
;
Arachidonic Acid
;
Edema
;
Free Radicals
;
Ischemia
;
Metabolism
;
Muscle, Skeletal
;
Neutrophils
;
Oxygen
;
Rats
;
Reperfusion
;
Reperfusion Injury*
;
Thioctic Acid*
;
Tourniquets