Interleukin 1(IL-1), a 17.5 KD glycoprotein, is known to be associated with local bone resorption. In the present study, we examined the effects of IL-1, compared with insulin and parathyroid hormone (PTH), on DNA, protein and collagen synthesis in UMR-106-01 rat osteoblastic osteosarcoma cells. When 200 units/mL IL-1 was administered to UMR-106-01 cells, [3H]-thymidine uptake increased to 119% of the untreated control. But when 10 nM insulin was added to the cells, [3H]- thymidine uptake increased to 130% and when 1 nM PTH was added, the uptake decreased to 89% of the control. On the other hand, protein and collagen synthesis, measured by [3H]-leucine and [3H]-proline incorporation respectively, were not affected by IL-1 administration compared to the other hormones. These results indicate that IL-1 effects osteoblast-like cells, stimulating DNA synthesis via a different mechanism to the well-known cell growth factor, insulin.
Animals ; Bone Resorption ; Cell Proliferation* ; Collagen ; DNA ; Glycoproteins ; Hand ; Insulin ; Interleukin-1* ; Interleukins ; Osteoblasts ; Osteosarcoma ; Parathyroid Hormone ; Rats ; Thymidine

No abstract available.
Cleft Lip ; Humans ; Orthognathic Surgery ; Palate

No abstract available.
Animals ; Cisplatin* ; Ditiocarb* ; Epithelium* ; Mice* ; Tongue*

No abstract available.
Angiofibroma* ; Estrogens* ; Progesterone* ; Receptors, Progesterone*

Ankylosing spondylitis (AS) is a chronic inflammatory disease presenting progressive spinal stiffness and sacroiliitis. Cervical spine fracture combined with AS should be treated with operation, but it is closely related with increased rates of surgical site infection, which are associated with an elevated erythrocyte sedimentation rate and elevated C-reactive protein. We report a case of delayed postoperative infection appeared in cervical paravertebral space, which was masked by laboratory findings and clinical characteristics represented in this rheumatic disease. A 53-year-old man who had medical history of AS got operation after cervical spine fracture. During hospitalization, he experienced aching pain originating from left posterior neck to shoulder, which was revealed out to be delayed postoperative infection, diagnostically obscured by elevated values of inflammatory markers. This case emphasizes detailed evaluation considering symptoms and comorbidity of the patient should be performed to apply proper management.

PURPOSE: This study was performed to identify the effects of selective norepinephrine reuptake inhibitor (SNRI, venlafaxine) on the urethral perfusion pressure (UPP) and also to assess its therapeutic potentials for stress urinary incontinence. MATERIALS AND METHODS: Smooth muscle strips of bladder and proximal urethra were prepared using female New Zealand white rabbits. Each strip was electrically stimulated and the degree of strip contraction was recorded by polygraph. After administration of venlafaxine (10(-6)M), responses of strips to electrical stimulation were measured. In separate experiments of eighty adult female Sprague Dawley rats, intravesical pressure and UPP was measured separately via double lumen catheter. Changes of intravesical pressure and UPP to intra-arterial (i.a.) and intra-urethral (i.u.) administra tion of phenylephrine, phentolamine, nitroprusside, fluoxetine (SSRIs), and venlafaxine were monitored, respectively. RESULTS: In strip study, pretreatment of venlafaxine significantly decreased the contrac tion of bladder strips (p=0.01) and significantly increased the contraction of urethral strips (p=0.008). In separate experiment of in vivo animal study, phenylephrine signifi cantly increased the UPP. Phentolamine (i.a.) significantly decreased the UPP (p= 0.001). UPP was also significantly decreased by intra urethral infusion of nitroprusside (p=0.003), but not significantly decreased by intra-arterial nitroprusside (p=0.23). Fluoxetine (i.a.) did not increase UPP significantly (p=0.15). Venlafaxine (i.a. and i.u.) significantly increased UPP (p=0.0002, 0.0001), respectively. In each case, intravesical pressure was not changed significantly. CONCLUSIONS: These results demonstrate that SNRI increases UPP in vitro and in vivo animal study. These results imply that venlafaxine may be useful for treatment of stress urinary incontinence by increasing UPP.
Adult ; Animals ; Catheters ; Electric Stimulation ; Female ; Fluoxetine ; Humans ; Muscle, Smooth ; Nitroprusside ; Norepinephrine* ; Perfusion ; Phentolamine ; Phenylephrine ; Rabbits ; Rats, Sprague-Dawley ; Urethra ; Urinary Bladder ; Urinary Incontinence ; Venlafaxine Hydrochloride

Myoclonus is an abrupt arrhythmic condition with shock-like movements that can be triggered by sensory stimuli, affecting the trunk or limbs during voluntary movement. Since motor symptoms are often not easily treatable, various pharmacological treatment options have been suggested. We report a case of using intrathecal baclofen (ITB) therapy in a patient with hypoxic brain injury (HBI), leading to the alleviation of myoclonus. A 29-year-old woman repeatedly presented with generalized myoclonus and multiple joint contractures at both upper and lower limbs after resuscitation. She cried during intractable myoclonus events, making it difficult for her to maintain a good sleep pattern. Due to the persistent status of multiple joint contractures and intractable myoclonus, we offered an ITB trial to control her symptoms. After ITB, her total scores on the Unified Myoclonus Rating Scale progressively improved as the doses of baclofen increased. Therefore, ITB therapy should be considered as a substantial option in the management of intractable myoclonus in patients with HBI to prevent various complications and improve the quality of life.

OBJECTIVES: This study investigated the question of whether adenoviral magnetofection can be a suitable method for increasing the efficacy of gene delivery into bone marrow stromal cell (BMSC) and for generation of a high level of bone morphogenic protein (BMP) secretion at a minimized viral titer. MATERIALS AND METHODS: Primary BMSCs were isolated from C57BL6 mice and transduced with adenoviral vectors encoding beta galactosidase or BMP2 and BMP7. The level of BMP secretion, activity of osteoblast differentiation, and cell viability of magnetofection were measured and compared with those of the control group. RESULTS: The expression level of beta galactosidase showed that the cell transduction efficiency of AdLacZ increased according to the increased amount of magnetic nanoparticles. No change in cell viability was observed after magnetofection with 2 microL of magnetic nanoparticle. Secretion of BMP2 or BMP7 was accelerated after transduction of AdBMP2 and 7 with magnetofection. AdBMP2 adenoviral magnetofection resulted in up to 7.2-fold higher secretion of BMP2, compared with conventional AdBMP2-transduced BMSCs. Magnetofection also induced a dramatic increase in secretion of BMP7 by up to 10-fold compared to the control. Use of only 1 multiplicity of infection (moi) of magnetofection with adenoviral transduction of AdBMP2 or AdBMP7 resulted in significantly higher transgene expression compared to 20 moi of conventional adenoviral transduction. CONCLUSION: Magnetic particle-mediated gene transudation is a highly efficient method of gene delivery to BMSCs. Magnetofection can lower the amount of viral particles while improving the efficacy of gene delivery.
Adenoviridae ; Animals ; beta-Galactosidase ; Bone Marrow ; Cell Survival ; Magnetics ; Magnets ; Mesenchymal Stromal Cells ; Mice ; Nanoparticles ; Osteoblasts ; Transgenes ; Virion

Fine needle aspiration cytologyof a pulmonary mass was performed on a 51-year-old man who had a left testicular mass. Cytologic features were composed of a homogeneous population of malignant cells associated with a background of foamy and lacelike material. The cellular features were characterized by monomorphous cell proliferation of relatively regular large cells, generally isolated or grouped. Occasionally, fine branching stroma with large tumor cells and scanty lymphocytes were noted. The tumor cells had a round, regular nucleus, prominent round nucleoli, and a thin rim of cytoplasm containing large vacuoles or lacunae filled with glycogen. The fine needle aspiration cytologic diagnosis was highly consistent with metastatic seminoma from testis and less likely primary or other metastatic carcinoma. The diagnosis of resected testicular mass was classic seminoma. Despite the fact that cytopathologists were not familiar with diagnosis of seminoma due to clinician's lack of interest in fine needle aspiration cytology of germ cell tumors including seminoma, it appears that a diagnosis of this tumor should not be problematic in cytologic material if specific histologic criteria are applied.
Biopsy, Fine-Needle* ; Cell Proliferation ; Cytoplasm ; Diagnosis ; Glycogen ; Humans ; Lung ; Lymphocytes ; Middle Aged ; Neoplasm Metastasis ; Neoplasms, Germ Cell and Embryonal ; Seminoma* ; Testis ; Vacuoles

Regardless of the method of treatment, as many as 5% of all pelvic fractures result in malunion or nonunion of the pelvis. However, there is not much information in the literature on the management of these late complications. Because they cause disabling symptoms and socioeconomic problems, some patients with malunion or nonunion of pelvic fractures need to undergo surgery. We report our experience with satisfactory results of surgery for pelvic malunion and nonunion in four patients. The key to successful reconstruction is thorough preoperative planning and methodical surgical intervention.
Adult ; Female ; Fracture Fixation, Internal/*instrumentation/*methods ; Fractures, Malunited/radiography/*surgery ; Fractures, Ununited/radiography/*surgery ; Humans ; Pelvic Bones/injuries/radiography/*surgery