OBJECTIVE: To compare the ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 in clinic. METHOD: The stability of ELISA reagent was studied first with parameters of intra and inter assay variations (%CV). Calibration curve of the two reagents was compared. Sixty-five serum samples before or after standardized specific immunotherapy were examined with the two methods, correlation coefficient was calculated. RESULT: The stability of ELISA reagent was good with low intra and inter assay variations (3.48% and 5.78%). Calibration curve of the two reagents was similar. The correlation coefficient was 0.93 between the two test methods. CONCLUSION The results of ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 were highly consistent. The ELISA reagents could be applied in clinic.
Animals ; Calibration ; Enzyme-Linked Immunosorbent Assay ; methods ; Humans ; Immunoglobulin G ; blood ; Indicators and Reagents ; Pyroglyphidae ; immunology