Objective To observe the effects of Qingjin Huatan Decoction on EGFR/MAPK signaling pathway of airway mucus hypersecretion rats with chronic obstructive pulmonary disease (COPD). Methods Intratracheal instillation of LPS combined with smudging method was used to establish COPD airway mucus hypersecretion rat models. Experimental rats were randomly divided into blank group, model group, Qingjin Huatan Decoction group and clarithromycin group. The blank group was normally fed, while the other three groups were given NS, Qingjin Huatan Decoction, and clarithromycin respectively for gavage, once a day for 30 days. All rats were killed on the 31st day, and pathological changes of lung tissue and mucous glands hyperplasia were observed by HE staining method. The gene expressions of EGFR and MUC5AC in lung tissue were detected by RT-PCR method. The protein expressions of P-EGFR, P-ERK, P-JNK, P-p38 and MUC5AC in pulmonary tissue and airway epithelium were detected by immunohistochemical method. Results Compared with the blank group, mucous glands hyperplasia on airway epithelium, protein expressions of P-EGFR, P-ERK, P-JNK, P-p38 and MUC5AC on airway epithelium significantly increased in the model group (P<0.01); gene expression of MUC5AC of lung tissue increased (P<0.05). Compared with the model group, mucous glands hyperplasia on airway epithelium, P-p38, P-ERK and MUC5AC protein expression on airway epithelium in Qingjin Huatan Decoction group significantly decreased (P<0.05, P<0.01); the protein expression of P-JNK increased significantly (P<0.01). EGFR and MUC5AC mRNA in lung tissue in Qingjin Huatan Decoction group decreased significantly (P<0.01). Conclusion Qingjin Huatan Decoction can reduce airway mucus hepersecrection of COPD by inhibiting ERK and p38 signal pathway on EGFR downstream.

Objective To explore the mechanism ofJiedu Qingfei Mixture for airway mucus hypersecretion of rat models with chronic obstructive pulmonary disease (COPD).Methods Airway instilling lipopolysaccharide combining fuming method was used to establish COPD models. Forty clean level Wistar strain rats were randomly divided into blank control group, model group,Jiedu Qingfei group, and clarithromycin group. Model group, Jiedu Qingfei group, and clarithromycin group were given normal saline,Jiedu Qingfei Mixture, and clarithromycin by gavage respectively, while the blank control group was raised normally for 30 d. All rats were killed on the 31st day for taking lung tissue (6 rats from each group were chosen randomly). Pathological changes of lung tissue and mucous glands hyperplasia were observed by HE staining method. NE and MUC5AC mRNA expression on lung tissue were detected by RT-PCR method. Protein expressions of NE and MUC5AC on pulmonary tissue and airway epithelium were detected by immunohistochemical method.Results Compared with blank control group, mucous glands hyperplasia on airway epithelium, mRNA expression of NE and MUC5AC in lung tissue, and protein expressions of NE and MUCA5C on airway epithelium in the model group significantly increased (P<0.05,P<0.01). Compared with model group, mucous glands hyperplasia on airway epithelium inJiedu Qingfei group significantly decreased (P<0.01), as same as clarithromycin group;Jiedu Qingfei group showed better effects on down-regulating NE and MUC5AC mRNA expression in lung tissue compared with clarithromycin group. MUC5AC protein expression on airway epithelium inJiedu Qingfei group significantly decreased (P<0.05), as same as clarithromycin group.Jiedu Qingfei group and clarithromycin group had no difference on NE protein expression in airway epithelium compared with model group.Conclusion Jiedu Qingfei group Mixture can reduce airway mucus hypersecretion of COPD by down-regulating MUC5AC expression through neutrophil elastase.

Objectives:To study the clinical significance of electron beam CT with three-dimensional reconstruction in evaluating acetabular fractures.　Methods:Twenty-six cases of acetabuar fractures were examined under X-ray and 3D reconstruction. Degrees of displacement were evaluated and classified for the selection of therapy.　Results:The 26 cases of acetabuar fractures were: 1 case of anterior column fracture,3 cases of posterior column fracture,11 cases of posterior wall fracture,4 cases of transverse fracture, and 7 cases of compound fracture. All the 26 cases were accurately evaluated by EBCT 3D image, according to the image, appropriate surgical treatment were applied to 15 cases, and 11 cases were treated nonoperatively.　Conclusions:EBCT 3D reconstruction could revealed the characteristics of acetabular fractures, which aided the evaluation and selection of therapy, increased the veracity of procedures and reduced the unnecessary operations.

Objective To explore the effects of Sishen Pill and Gegen Qinlian Tablet on cytokines of acute and chronic colitis induced by dextran sulfate sodium (DSS) in mice. Methods Mice freely drank 4%DSS dissolved in drinking water for continuous 5 days to establish acute colitis model. Mice circularly drank 3% DSS for 4 times for the establishment of chronic colitis model. In the acute or chronic colitis experiment, mice were randomly divided into control group, acute and chronic model groups, Sishen Pill group, and Gegen Qinlian Tablet group. Acute model group was administrated one day after DSS drinking for 8 days. Chronic model group was administrated after the second time of circular drinking for 16 days. ELISA was used to detect the contents of IFN-γ, IL-17 and IL-22 in cultural supernatant of mouse colon. Results Contents of IFN-γ, IL-17A and IL-22 in cultural supernatant increased significantly in acute models (P<0.01). Gegen Qinlian Tablet can significantly decrease the contents of IFN-γ and IL-17A of acute models (P<0.05). Sishen Pill can significantly decrease the content of IL-17A (P<0.05). IFN-γand IL-17A in cultural supernatant in chronic model mice significantly increased compared with control group (P<0.01). Sishen Pill and Gegen Qinlian Tablet inhibited the contents of IFN-γ and IL-17A. Compared with control group, Sishen Pill significantly increased the content of IL-22 (P<0.05). Conclusion Sishen Pill and Gegen Qinlian Tablet can treat colitis by decreasing the contents of IFN-γ and IL-17A in acute colitis model mice;Sishen Pill can treat chronic colitis by promoting IL-22 to increase.

Objective To explore the effects ofDachengqiDecoction on morphological changes, lung and spleen indexes, and total IgE levels of mice with allergic asthma; To discuss it relevant mechanism of action.MethodsThe method of OVA sensitization and provocation was used to establish allergic asthma mice models. 20 C57BL/6 mice were randomly divided into control group, model group,Dachengqi Decoction group and dexamethasone positive group. Each medication group was given relevant medicine for gavage. Lung and spleen indexes in each group were detected. The total IgE levels in serum of mice were detected by ELISA, and HE staining was used to conduct pathological observation.Results Compared with control group, the lung index and level of IgE in model group increased significantly and pulmonary histological results showed abundant inflammatory cells infiltrated in the bronchus; compared with the model group, the lung index as well as the levels of IgE decreased significantly in the Dachengqi Decoction and dexamethasone positive group (P<0.05), and the lung tissue organization form was improved significantly.ConclusionDachengqi Decoction has inhibiting effects on pulmonary inflammation of mice with asthma.

Objective To investigate the effective mechanism of Jiawei Maxing Shigan Tang(JMST, Modified Decoction of Ephedra,Apricot Kernel,Gypsum and Licorice)in treatment of acute exacerbated chronic obstructive pulmonary disease(AECOPD).Methods Wistar rats(n =40)were randomly divided into blank control group,model group,JMST group and clarithromycin group.The blank control group was given normal feeding, and model group, JMST group and clarithromycin group were given airway instillation of lipopolysaccharide(LPS)and smudging for establishing AECOPD model.The model group was given normal saline,JMST group,JMST(8.7 g/kg)and clarithromycin group,clarithromycin suspension(50 mg/kg)continuously for 30 d.The lung function was measured on the 31std.The lung tissue was collected to making pathological sections for observing goblet cells after AB -PAS staining.The expressions of mucoprotein 5AC(MUC5AC)and neutrophil elastase(NE)were detected by using immunohistochemistry technique.Results Compared with blank control group, the ratio of 0.3-second forced expiratory volume to forced vital capacity(FEV0.3/FVC)decreased significantly, and number of goblet cells and expressions of MUC 5AC and NE increased significantly in model group,JMST group and clarithromycin group.Compared with model group, FEV0.3/FVC increased significantly,and number of goblet cells and expressions of MUC 5AC and NE decreased significantly in JMST group.The comparison in FEV0.3/FVC,number of goblet cells and expressions of MUC 5AC and NE had no statistical difference between JMST group and clarithromycin group.Conclusion Jiawei Maxing Shigan Tang can significantly promote FEV0.3/FVC, inhibit proliferation of goblet cells, down-regulate expressions of MUC5AC and NE and relieve airway mucus hypersecretion in AECOPD rats, which may be its one of therapeutic mechanisms in treatment of AECOPD.

Objective To study the different prescriptions of eletcro-acupuncture ( distal-proximal acu-points, proximal acupoints and distal acupoints) on pain-related behaviors and expression of microglia marker OX42 and cyclooxygenase 2 ( COX2 ) in spinal dorsal horn in rat model of transplantation of autol-ogous nucleus pulposus, so as to provide experimental basis for the mechanism of the different prescrip-tions. Methods 54 healthy male SD rats were randomly divided into six groups ( each n=9 )-normal group, model group, sham operation group, distal-proximal acupoint group, proximal acupoint group, distal acupoint group. After modeling, the rats in three acupuncture groups were treated for 20 minutes, once daily for consecutive 7 days. Those in distal-proximal acupoint group were needled at bilateral “L5 Jiajixue” ( Ex-B2 ) ,“Dachangshu” ( BL 25 ) ,“Weizhong” ( BL 40 ) and“Kunlun” ( BL 60 );those in proximal acupoint group were needled at bilateral“L5 Jiajixue” ( Ex-B2 ) and“Dachangshu” ( BL 25 ) ;the rats in distal acupoint group rats were needled at bilateral “Weizhong” ( BL 40 ) and “Kunlun” ( BL 60). Thermal withdrawal latencies were evaluated. The expressions of OX42 and COX2 in the left spinal dorsal horn were detected using immunohistochemical methods. Results Thermal withdrawal latencies and the expression of OX42 and COX2 increased in rat model of transplantation of autologous nucleus pul-posus. After treatment, thermal pain threshold was improved (P<0. 05), and the expression of OX42 and COX2 were inhibited ( P<0 . 05 ) . The inhibitory effects of distal-proximal acupoint group and proxi-mal acupoint group were superior to that in distal acupoint group. Conclusion Each acupuncture pre-scription reduced anti-inflammatory effects by inhibiting inflammatory signaling pathways in spinal dorsal horn, with the prescription of distal-proximal acupoints and proximal acupoints superior to distal acu-points.

Objective:To compare the aortic remodeling of the Fabulous stent system and standard thoracic aortic endovascular repair (TEVAR) on distal aorta type B aortic dissection (TBAD). Methods:The prospective data collected between Dec 2017 and Oct 2019 from 134 patients with type B aortic dissection (TBAD) who underwent treatment with the "Fabulous" stent system, and retrospective data from 159 TBAD patients receiving standard TEVAR from corresponding multicenter. By using propensity score matching analysis, we compared the prognosis and aortic remodeling outcomes in patients undergoing Fabulous and standard TEVAR treatments during a 1-year postoperative follow-up.Results:In this study, 62 patients in Fabulous group and 62 patients in standard TEVAR were included.There were no significant statistical differences in baseline characteristics between the two groups. In terms of aortic remodeling in bare stent region, Fabulous group had better change trends of diameter of true lumen [10.6 (4.4, 14.5) mm vs. 4.7 (0.9, 10.7) mm, P=0.001] and false lumen [-24.2 (-30.5, -4.9) mm vs. 0.7 (-11.8, 2.3) mm, P<0.001] than those in the standard TEVAR group. The rate of complete false lumen thrombosis was also higher in the Fabulous group (62.9% vs. 37.1%, P=0.042). Conclusion:The Fabulous stent system, when compared to standard TEVAR surgery, demonstrates good aortic remodeling outcomes in the distal aorta.