1.A Preliminary Analysis of Teaching Strategy in Clinical Research Introduction of Rehabilitation
Chinese Journal of Rehabilitation Theory and Practice 2007;13(8):791-792
The top priority to develop rehabilitation medicine in our country is rehabilitation professional training. The important way of rehabilitation professtional training is undergraduate course education of rehabilitation treatment. Great attention of clinical science research should be paid in rehabilitation professtional training. Teaching strategy of clinical science research introduction should include: arousing the interest of learning and developing quality of science research, mastering basic steps of science research, encouraging learning of new konwledge, paying attention to evidence-based rehabilitation medicine, reforming examination form and valuing the ability of training.
2.A Preliminary Analysis of Teaching Strategy in the Course of Environmental Adaptation
Chinese Journal of Rehabilitation Theory and Practice 2015;21(8):990-992
Close attention of the Course of Environmental Adaptation should be paid in rehabilitation professional training. Its teaching strategy should include: arousing the interest of learning, and developing the working habits concerning the environmental condition of the patients; mastering the international standard of barrier- free design; encouraging researching of practical design of environmental adaptation, emphasizing the intern operation training of environmental adaptation, and concerning the universal education of the knowledge of barrier- free design.
3.An Introduction of the Quadriplegia Index of Function
Chinese Journal of Rehabilitation Theory and Practice 1996;2(1):18-25
Presented in this paper is an introduction of the Quadriplegia Index of Function,it has tenitems,including transfer,grooming,bathing,feeding,dressing and undressing,wheelchair propulsion,bed mobility,bladder function,bowel function and self一care information. Overall is one hundred degree. This evaluation form is comprehensive and rational,it is sensitive and effective to dOcument the small butsignificant functional gains made by quadriplegics during medical rehabilitation.
4.Effects of drugs on the functions of mitochondria in retinal nerve cells
Genlin LI ; Jinjin WANG ; Yueyue LIU
Chinese Journal of Ocular Fundus Diseases 2000;0(04):-
Objective To explore the effects of drugs on functions of mitochondria in retinal nerve cells, and to lay a foundation of the investigation of drug protection for retinal nerve cells. Methods Cultivation of the retinal nerve cells of 8 eyes of neonatal calves was performed. The changes of fluorescent density of the mitochondria of cultured cells labeled by dye rhodamine 123 (Rh123) before and after the activation of the medicines, including ferulic acid (FA), arginine, glycine, taurine, vitamine E and brain-derived neurotrophic factor (BDNF) respectively, were detected by laser-scanning confocal microscopy. Results FA with the concentration of 500 ?g/ml led the diphasic variation of the fluorescent intensity of mitochondria. After scanning for 60.772 seconds when treated with FA firstly, the fluorescent intensity decreased rapidly (from 45.425?4.153 to 22.135?5.293); while after 112.774 seconds when treated secondly, the intensity increased obviously (from 19.655?4.383 to 28.247?4.764), and after 168.773 seconds when treated thirdly the intensity still increased. After scanning for 56.457 seconds when treated with vitamin E (12.5 mg/ml), the fluorescent intensity increased obviously (from 88.255?5.039 to 111.273?4.529), which suggested that vitamin E with the concentration of 12.5 mg/ml strengthen the fluorescent intensity. After scanning for 58.147 and 134.148 seconds when treated with BDNF (50 ng/ml) respectively, the fluorescent intensity increased obviously (from 69.115?5.038 to 77.225?5.131) which suggested that BDNF with the concentration of 50 ng/ml led the increase of the fluorescent intensity. Glycine (2.5 mg/ml) and arginine(30 mg/ml) didn't affect the fluorescent intensity of mitochondria, and taurine (6.25 mg/ml) caused the appreciable decrease of the fluorescent intensity. Conclusion FA, BDNF and vitamin E may promote the metabolism of retinal nerve cells via the path of mitochondria, while amino acids may adjust the activation of retinal nerve cells through other ways.
5.Clean intermittent catheterization of spinal cord injured patients
Zhiwen WU ; Xuemei LIU ; Genlin LIU ; Hongjun ZHOU
Chinese Journal of Rehabilitation Theory and Practice 2003;9(2):91-93
ObjectiveTo investigate the nursing management of clean intermittent catheterization (CIC) of spinal cord injured (SCI) patients.Methods72 SCI patients were randomly divided into two groups: CIC group (37 cases) and sterile intermittent catheterization (SIC) group (35cases); urine cultures were performed before and after catheterization in each group, hand cultures were also performed before catheterization only in CIC group. Results10 cases of CIC group and 11 cases of SIC group had different results of urine culture before and after intermittent catheterization, but there was no statistical difference between two groups (P>0.05). 5 cases had positive results of hand culture, but the colony counting was in the normal range. Follow up was made six months after intermittent catheterization. There were 35 cases (94.59%) in CIC group and 33 cases (94.29%) in SIC group who could urinate regularly with post void residual less than 50 ml. One case in each group had symptomatic urinary tract infection respectively. ConclusionsCIC is a simple, safe and effective method to resolve the bladder dysfunction of SCI patients.
6.Three kinds of skin flaps for hand wound repair:an effectiveness evaluation
Hongda LV ; Genlin WANG ; Yuefei LIU ; Jun ZOU
Chinese Journal of Tissue Engineering Research 2015;(24):3929-3936
BACKGROUND:Donor site, recipient site and operator are three important factors for flap transplantation in the repair of hand soft tissue defects. Depth studies are needed on how to choose flaps according to the principle of“good reconstruction of the recipient site, little damage to the donor site, reliable survival, simple and easy operation”. OBJECTIVE:To compare the repairing effects of pedicled neurovascular fasciocutaneous flap, abdominal skin flap and free flap, thereby providing a reference for clinical choice. METHODS:Twenty-eight patients who underwent transplantation of pedicled neurovascular fasciocutaneous flap, abdominal skin flap and free flap in the repair of hand defects, respectively, were al fol ow-up to compare and analyze the disability of the arm, shoulder and hand (DASH), injured site, touch and pressure sensation (Semme-Weinstein monofilament method), dynamic and static two-point discrimination (m2PD, s2PD), temperature sensation, swel ing degree, and degree of scar contracture at donor sites. RESULTS AND CONCLUSION:No significant difference in the degree of scar contracture, temperature sensation, touch sensation, and two-point discrimination was found among the three kinds of flaps, but DASH score, swel ing degree and tough and pressure sensation in the abdominal skin flap group were significantly different from those in the other two groups. These findings indicate that these three kinds of flaps have their own characteristics, and can be selected clinical y based on a variety of objective requirements.
7.Experimental study on cultivation of adult human retinal neural cells
Zhijun SHEN ; Genlin LI ; Jinjin WANG ; Yueyue LIU
Ophthalmology in China 2006;0(06):-
Objective To establish a culture system in vitro of adult human retinal neural cells for providing a model for the research of retinal neural cells. Design Experimental study. Participants Cultured adult human retinal neural cells. Methods The isolated cells from adult human postmortem retina (20?40 years old) were cultured, then cells of different stages were identified with immunocytochemical staining and judged with phase contrast microscopy and electron microscopy. Main Outcome Measures Cellular morphology and structure. Results (1) The results of cell culture: the adult retinal neural cells could survive in vitro under some conditions and were identified as NSE positive mostly. (2) The results of electron microscopy: most cultured cells were photoreceptors, bipolar cells, horizontal cells and some were glial cells with scanning electron microscopy. Conclusions Under feasible conditions, the adult human retinal neural cells could be cultured and maintained effectively in vitro.