A 41-year-old man presented a bean sized, solid, painless left scrotal mass he'd had for 10 years. The mass was well demarcated and it showed homogeneous echogenecity on ultrasonography. Simple mass excision was performed and the specimen revealed a relatively well circumscribed mass lesion composed of dilated tubules with flattened lining cells. A focal infiltration to the seminiferous tubule and involvement of the biopsy margins were observed. The tubules had a positive reaction to the calretinin and anti-mesothelial antibody on immunohistochemical stain. Therefore, the tumor was diagnosed as adenomatoid tumor of the testis. Adenomatoid tumors of testis are rare benign neoplasms that are thought to originate from mesothelum. Most cases were reported in the epididymis, spermatic cord and testicular tunica, and rare cases were from the ejaculatory duct, prostate and adrenal gland. The findings of adenomatoid tumor infiltrating through the testis parenchyme and seminiferous tubule, like for our case, have not been previously reported in Korea.
Adenomatoid Tumor* ; Adrenal Glands ; Adult ; Biopsy ; Calbindin 2 ; Ejaculatory Ducts ; Epididymis ; Humans ; Korea ; Male ; Prostate ; Seminiferous Tubules* ; Spermatic Cord ; Testis* ; Ultrasonography

A number of tissues have been studied in the past with respect to their organ-specific antigens. In many instances it has been possible to produce autoantibodies against characteristic components. The testis, epididymis, and seminal plasma have been largely explored from this angle. Interest in the field of accessory glands began many years ago, when the first cross-reactions between extracts of prostate, seminal vesicles and seminal plasma were demonstrated. As a consequence, the possibility that some seminal plasma antigens might be present in the accessory glands before being secreted into the genital tract opened up a new approach to possible autoimmunologic damage of these glands and of seminal spermatozoa as well. The purpose of this study is made to observe the effect of homologous epididymal extracts on the spermatogenesis in mouse. Isoimmunization with extracts of mouse epididymis, administered with complete Freund`s adjuvant, has been performed in this study.The results were as follows: 1. The histological observations revealed that spermatogenesis was adversely affected by the immunization with homologous epididymal extract added with an equal amount of complete Freund's adjuvant for 6 weeks. It was observed that spermatogenesis was remarkably impaired in the experimental group whereas it was unaffected in the control group of male mouse. The results further indicated that the degeneration and exfoliation were found in the germinal cell of seminiferous tubules and in the epithelium of the epididymal ducts besides intercanalicular infiltration of m0nonuclear round cells. 2. The cross-reactions between extracts of epididymis and testicular tissues were demonstrated in mouse. 3. The immunological examination such as immune diffusion test and sperm agglutination test showed negative reaction on all of the experimental animals in this study. Therefore. the immunological change in this experiments seems to be caused by cell mediated immunity.
Animals ; Autoantibodies ; Diffusion ; Epididymis ; Epithelium ; Freund's Adjuvant ; Humans ; Immunity, Cellular ; Immunization ; Male ; Mice* ; Prostate ; Semen ; Seminal Vesicles ; Seminiferous Tubules ; Sperm Agglutination ; Spermatogenesis* ; Spermatozoa ; Testis

Evidence of antigenicity of testis and semen has been presented since Landsteiner (1899), Metchinikoff (1900) and Metalnikoff (1900) first demonstrated the induction of a spermatoxic antibody in animals sensitized with testicular homogenates or semen. Interest in the field of male accessory sex gland began longtime ago, when the first cross-reaction between extracts of prostate, seminal plasma were demonstrated. Saline extracts of prostatic secretion from bulls, tested by double agar diffusion technique showed four antigens common to serum proteins and spermatozoa. The seminal vesicle have been found to have three to five antigens, also with common reactivity to spermatozoa. Attempts have been made to induce cross-immunologic damage in the testes by repeated immunization of mice with epididymal extracts (free of sperm) plus adjuvant and it was claimed that spermatogenesis was adversely effected and fertility of females was markedify reduced following mating with immunized male (Shethye and Rao, 1968; Kim and Kim, 1982). Rabit antiserum produced against the tissue protein of rat epididymis and seminal vesicle was capable of immobilizing and agfflutinating the sperm of both animals and the rabbit antiserum against complex antigen of epididymal tissue protein and seminal vesicle tissue protein of rat was most potent on sperm immobilization and agglutination of both animals (Cha and Kim, 1975). The purpose of this study is to observe the effect of rabbit anti-rat epididymal serum on epididymis and spermatogenesis in rat. The results were as follow; 1. The intraluminalspermatozoa of epididymis were decreased in number but immature sperm cells were much more noted than normal control group. The interspaces of epididymal ducts were widened and infiltrated with mononuclear cells and congestion in some places. There was no definite degenerative changes on epididymal epithelium. 2. Spermatogenesis was mildly to moderately impaired in the experimental group whereasit was unaffected in the control group. Degeneration and exfoliation were found in the germinal cells of seminiferous tubules. Intraluminal Spermatozoa of seminiferous tubules were decreased in number.
Agar ; Agglutination ; Animals ; Blood Proteins ; Diffusion ; Epididymis* ; Epithelium ; Estrogens, Conjugated (USP) ; Female ; Fertility ; Humans ; Immobilization ; Immunization ; Male ; Mice ; Prostate ; Rats* ; Semen ; Seminal Vesicles ; Seminiferous Tubules ; Spermatogenesis ; Spermatozoa ; Testis

Condyloma acuminatum is a common lesion of the penis and genitalia. It uncommonly involves the urethra but when it does the lesions are difficult to eradicate. Treatment has included excision, fiuguration, podophyline and thiotepa0 none of which has been entirely successful. We report a case of intraurethral condyloma acuminatum treated with thiotepa instillation in 27 years old male patient
Adult ; Genitalia ; Humans ; Male ; Penis ; Thiotepa* ; Urethra

Median raphe cyst is an uncommon condition occuring on the ventral median raphe from the glans penis to the anus and represents a defect in the embryologic development of the male genitalia. The cyst wall may derive from endoderm, ectoderm, or mucous glands which are a normal constitute of the male urethra. It is classified as either a dermoid cyst lined by stratified squamous epithelium of ectodermal origin or a mucous cyst lined by cuboidal or columnar epithelium of endodermal origin. Surgical excision is the treatment of choice. We report a case of an 11-year old boy with three median raphe cysts of the scrotum. The epithelial lining of the cysts was mainly composed of pseudostratified columnar cells with decapitation secretion and focally showed stratified squamous cells and a transitional zone of two types of cells. Immunohistochemically, pseudostratified columnar cells showed CK 7 and CK 13 positivity, but were negative for CK 20. Besides, stratified squamous cells were negative for CK 7, CK 13 and CK 20. We report a rare case of median raphe cyst that is a combined type of dermoid cyst and mucous cyst.
Anal Canal ; Child ; Decapitation ; Dermoid Cyst ; Ectoderm ; Endoderm ; Epithelium ; Genitalia, Male ; Humans ; Male ; Penis ; Scrotum* ; Urethra

Male pseudohermaphroditism is not commonly reported in veterinary medicine. Here, a 3-year-old Maltese/poodle mixed dog presented with malformed external genitalia and episodic hematuria. Inspection and palpation of the external genitals showed a malformed penis, shortened prepuce, external urethral orifice, and cryptorchidism. There was no urethral meatus at the tip of the penis. The urethral opening was situated between the prepuce and the penis. The anterior half of the prepuce was absent, and the penis was free and exposed to both trauma and licking. Plain radiographic examination showed absence of an os penis in the penis. A double-contrast cystograph showed the suspected uterus as well as the cystic calculi. A hypoechoic space was seen at the dorsal portion of the urinary bladder. The space was suspected to be the uterus. A sagital ultrasonograph showed cystic calculi in the urinary bladder. During surgery to remove cystic calculi, hypoplastic testes as well as the uterus were observed. Histological examination of the testes showed the seminiferous tubules and interstitial cells. The sertoli cells and spermatogonia were adjacent to the basement membrane. No evidence of spermatogenesis was found. Striated squamous epithelial cells and smooth muscle cells were found in the uterus. This dog had vestigial oviducts as well as a uterus with male-appearing external genitals.
46, XY Disorders of Sex Development* ; Animals ; Basement Membrane ; Calculi ; Child, Preschool ; Cryptorchidism ; Disorders of Sex Development ; Dogs* ; Epithelial Cells ; Genitalia ; Hematuria ; Humans ; Male ; Myocytes, Smooth Muscle ; Oviducts ; Palpation ; Penis ; Seminiferous Tubules ; Sertoli Cells ; Spermatogenesis ; Spermatogonia ; Testis ; Urinary Bladder ; Uterus ; Veterinary Medicine

It is highly desirable to achieve optimal reproductive performance, reliable morphological and physiological basic data of the reproductive organs. Therefore, seasonal changes in serum testosterone, LH, and FSH concentrations, and morphological changes in testicular epithelial cells were studied in the Korean native pheasant throughout the annual cycle. Mature male pheasants[14-16 months after hatching] were used in this study. The general morphological changes of the epithelia of the seminiferous tubules were observed by dibasic stain, and semithin section from Epon blocks with a phase contrast microscopy. The ultrastructural changes of the the epithelia of the seminiferous tubules were investigated by ultrathin section with transmission electron microscope. The changes in the profiles of the serum FSH, LH, and testosterone concentratioins were measured by RIA[radioimmunoassay]. The results obtained are summarized as follows : 1. There was little variation in the average diameter of the seminiferous tubules from autumn[67.13+/-5.95micrometer] to winter[68.59+/-6.07micrometer], but the highest levels were reached in spring[192.78+/-41.58micrometer]. Thereafter, the diameter decreased slowly in summer[146.57+/-43.68micrometer], then decreased significantly in autumn[67.13+/-5.95micrometer]. 2. Serum testosterne concentration was low from autumn[13.+/-7.21ng/100ml] to winter[17.39+/-13.75ng/100ml], but the highest levels were reached in spring[127.72+/-66.47 ng/100ml]. Thereafter, the concentration was lowest in autumn[13.+/-7.21ng/100ml]. 3. Serum LH concentration increased slowly and linealy from autumn[5.04+/-1.04ng/100ml] to winter[6.23+/-1.08ng/100ml], but the highest levels were reached in spring[11.3+/-3.6 ng/100ml]. Thereafter, the concentration reached the lowest level in autumn[5.04+/-1.04 ng/100ml]. 4. Serum FSH concentration was low from autumn[4.65+/-0.63ng/100ml] to winter[4.2+/-0.98ng/100ml], but the highest levels were reached in spring[17.41+/-8.35ng/100ml]. Thereafter, concentration was the lowest in autumn[4.65+/-0.63ng/100ml]. 5. The seminiferous tubules showed the onset of the spermatongenic cycle in spring but the seminiferous tubules collected in summer exhibited partially degenerative changes. 6. The cytoplasmic process of Sertoli cells of the seminiferous tubules of the pheasant were collected in summer. Many vesicles and degeneratiye changes were included but many number of spermatozoa were embedded partially in the multivesicular bodies in these processes. 7. The diameter of the seminiferous tubules of the pheasant narrowed markedly in autumn, and atrophied in winter. The spermatogonia and Sertoli cells were arranged in monolayer. 8. The myelin figures in the cytoplasmic process of Sertoli cells of the seminiferous tubules of the pheasant in autumn. The nucleus of the Sertoli cells were of a round configuration elongated and oriented perpendicularly to the basement membrane. The results obtained provide basic data for reproductive physiology and are useful for studying the male genital organs of the Korean native pheasant. Structural changes of the seminiferous epithelial cells significantly and postively correlated with serum FSH, LH. The correlation of changes in the hormonal status with alterations of Sertoli cell organells precedes the breeding season.
Basement Membrane ; Breeding ; Cytoplasm ; Epithelial Cells ; Epithelium* ; Genitalia, Male ; Humans ; Male ; Microscopy, Phase-Contrast ; Multivesicular Bodies ; Myelin Sheath ; Physiology ; Seasons* ; Seminiferous Tubules* ; Sertoli Cells ; Spermatogonia ; Spermatozoa ; Testosterone*

Testosterone is required for the development and maintenance of the male accessory sex organs and their normal function. And it was reported that castration affect cells in the adult male rat accessory sex glands by induction of programmed cell death (apoptosis). So, in this study, the authors made an experiment to evaluate the effect of testosterone in the maure male rat penis and accessory sex glands following castration. Also, we utilized actinomycin D, a potent inhibitor of messenger and ribosomal RNA synthesis, in the experiment herein to assess the significance of regression process in the glands. Following are the changes in the serum testosterone level, the weight of the penis, ventral prostate and seminal vesicles and apoptosis occurrence of the control (castration, castration normal saline) and experimental (castration AD25, castration AD50) group of mature rats. 1. After castration, the control group and the experimental group showed decreased level of serum testosterone. 2. In the both groups, the weight of the penis, ventral prostate and seminal vesicles decreased gradually. 3. Compared to the control group, the castration AD25 did not show the inhibition of castration induced regression of penis and ventral prostate. However, castration AD50 showed the inhibition. 4 In the H-E staining and ApoTag in situ staining, the ventral prostate showed the most prominent apoptosis occurrence followed by the seminal vesicles and penis. These results suggest that after castration of the mature rat, due to testosterone deficiency, the weight of penis, ventral prostate and seminal vesicles decreased with the occurrence of apoptosis. Also, actinomycin D 50 micrometer seems to delay the regression process.
Adult ; Animals ; Apoptosis ; Castration* ; Cell Death ; Dactinomycin ; Genitalia ; Humans ; Male ; Penis* ; Prostate ; Rats* ; RNA, Ribosomal ; Seminal Vesicles ; Testosterone*

Median raphe canal is an uncommon disease and represents a defect abnormality of the male genitalia. It occurs along the ventral median raphe from the glans penis to the anus. Histologically the canal is lined by stratified squamous epithelium which dose not communicate with the urethra. Surgical excision is the treatment of choice. Recently we observed a 25-year-old male patient who had had a median raphe canal located in the ventral aspect of penis.
Adult ; Anal Canal ; Epithelium ; Genitalia, Male ; Humans ; Male ; Penis* ; Urethra

Median raphe canal is a uncommon disease and represents a defect in the embryologic development of male genitalia. It occurs most commonly near the glans penis, but may occur anywhere from the urethral meatus to the anus, and is lined by endodermal or ectodermal epithelium. Surgical excision is the treatment of choice. we report a median raphe canal on the ventral aspect of the penis and the scrotum with review of literatures.
Anal Canal ; Ectoderm ; Endoderm ; Epithelium ; Genitalia, Male ; Male ; Penis* ; Scrotum*