1.Incidence rates of HIV-1 epidemics among 4 high risk groups in Sichuan province during 2011-2015
Hong YANG ; Ling SU ; Li YE ; Lin XIAO ; Ying HU ; Maogang SHEN ; Dongbing WEI ; Dan YUAN ; Gengsheng ZHOU ; Honglu LIU ; Jia YU ; Shu LIANG
Chinese Journal of Epidemiology 2017;38(12):1649-1654
Objective Data from the HIV/AIDS surveillance sentinel sites was used to identify the recent HIV-1 infections and to estimate the HIV-1 incidence and epidemic trends among intravenous drug users (IDU),MSM,female sex workers and men attending the sexually transmitted disease clinics in Sichuan province.Methods Specimens collected from IDUs,MSM,female sex workers and men attending the sexually transmitted disease clinics sentinel sites were confirmed as HIV-I positive and tested with BED-CEIA.Annual HIV-1 prevalence and incidence rates were calculated and the incidence was adjusted by McNermar method.Results A total number of 194 223 serum specimens were collected and HIV antibody tested,with 5 297 HIV-1 positive.4 640 of the positives underwent BED-CEIA,with 749 identified as recent infections.During 2011-2015,HIV-1 incidence rates of the four types of population appeared as 5.16% (95%CI:4.65-5.66),0.22% (95%CI:0.16-0.28),0.57%(95%CI:0.45-0.69),7.53% (95%CI:6.06-9.01) and 0.44% (95%CI:0.36-0.53) respectively.Significant differences were seen in the HIV-1 incidence rates.Conclusions The HIV-1 incidence rates of IDUs from other sentinel sites in Sichuan province,female sex workers and men attending the sexually transmitted disease clinics appeared low,showing that the AIDS prevention and control activities worked effectively.However,the HIV-1 incidence of MSM was still at high level and even rising,suggesting that HIV-1 transmission between MSM and IDUs was possible.
2.Effect of Different Cultivation Modes on Microbial Diversity and Secondary Metabolites of Rhizosphere Soil of Gentiana crassicaulis
Wuni JIADUO ; Ami JIASHI ; Hongyu MA ; Gengsheng YE ; Chen CHEN ; Kaijun GOU ; Yan REN ; Mujia LA ; Rui ZENG
Chinese Journal of Experimental Traditional Medical Formulae 2023;29(10):192-201
ObjectiveTo investigate the effects of different cultivation modes on the yield of Gentiana crassicaulis and its microbial diversity and secondary metabolite content in the rhizosphere soil. MethodWith G. crassicaulis of different cultivation modes and its rhizosphere soil as the research objects, the composition of bacterial and fungal communities, dominant bacteria, and differential microorganisms in the rhizosphere soil were analyzed by high-throughput sequencing technology. HPLC was used to determine the content of iridoids in G. crassicaulis with different cultivation modes. ResultCompared with plastic film mulching, planting without mulch and intercropping of peony, white kidney bean, potato, and corn increased the yield of fresh products by 16.11%-17.68%, 22.48%-26.34%, 29.37%-32.19%, 34.82%-36.57%, and 35.34%-39.71%, respectively, and increased the yield of dry products by 19.75%-23.17%, 25.86%-29.32%, 30.18%-34.94%, 35.22%-39.87%, and 39.72%-43.73%. The total content of four iridoids, including gentiopicrin, loganic acid, sweroside, and swertiamarin, increased by 10.17%-37.83%, 5.93%-47.44%, 9.09%-28.84%, and 10.71%-28.57%, respectively. The diversity of bacterial and fungal communities in the rhizosphere soil increased significantly (P<0.05). The relative abundance of pathogenic bacteria such as Sordariomycetes, Leotiomycetes, Tremellomycetes, Eurotiomycetes, Fusarium, and Cladophialophora decreased, and the proportions of beneficial bacteria such as Proteobacteria, Acidobacteria, and Actinobacteriota increased and they gradually became the dominant bacteria. ConclusionDifferent cultivation modes can affect the yield of G. crassicaulis and its microbial diversity and iridoid content in the rhizosphere soil. Cultivation without mulch and intercropping patterns have certain advantages, which can provide theoretical references for the planting of G. crassicaulis.
3. An analysis on incidence of HIV-1 epidemics among men who have sex with men in Sichuan Province during 2011-2015
Hong YANG ; Li YE ; Ling SU ; Yang LIU ; Lin XIAO ; Ying HU ; Dongbing WEI ; Dan YUAN ; Gengsheng ZHOU ; Honglu LIU ; Jia YU ; Shu LIANG
Chinese Journal of Preventive Medicine 2019;53(3):327-329
The assay was used in HIV/AIDS surveillance sentinel sites to identify recent HIV-1 infection, to estimate HIV-1 incidence and understand the epidemic trends among men who have sex with men during 2011-2015. During 2011-2015, the HIV-1 incidence of men who have sex with men was 5.16%(95