Objectives To identify the prevalence and risk factors that were associated with poststroke cognitive impairment(PSCI)among a large cohort of consecutive ischemic stroke patients.Methods 526 consecutive patients.who had suffered from ischemic stroke 3 months ago were recruited in this study. Patients were classified as having no cognitive impairment(NCI),cognitive impairment but no dementia (CIND)and vascular dementia(VaD)according to their cognitive function.They were also categorized as with subcortical ischemic vascular diseases(SIVD)or cortical ischemic vascular diseases(CIVD)with neuroimaging findings.Their demographic data.vascular risk factors and stroke characteristics were also dacumented.Results The overall prevalence of PSCI(CIND+VaD)Was 36.7%.Compared with the NCI subjects.PSCI SObjects were older,more dominant femininely,less educated,with more cases of right hemi-paralysis and higher depression scores.but did not have more specific vascular risk factor.Separately,VaD patients demonstrated lower economic level,less spouse-caring,more prevalence of dysphasia,hisher rate of incontinence and more cases with CIVD.while CIND patients had more cases with SIVD.The VaD patients had more cortical lesions and lower depression scores than the CIND patients.On logistic regression analysis,older age,female gender,lower economic level,dysphasia,SIVD,CIVD and hisher depression scores were independent risk factors for PSCI.ConclusionPSCI is common among ischemic stroke patients and related to demographic factors.stroke types,and depression.

OBJECTIVEThe research on flavonoid in the recent years is extensive, such as Soybeans flavone, Baicalensis flavone, Epimedium flavone. Experiments show the effects of flavonoid is closely related to human health. There are a lot of reports about Ginkcobiobal flavone. In order to make further progress research on ginlcgobiobal flavone, we sum up the articles and reports on ginlcgobiobal flavone in the recent years.

METHODTo searche the articles about ginlcgobiobal flavone studies in the past five years.

RESULTGinlcgobiobal flavone is not only a vasodilator, but also has the effects of anti-inflammation, analgesia, lowering blood lipids, preventing senile and inhibiting tumor, treating leukaemia, regulating gene and biotransformation.

CONCLUSIONGinlcgobiobal flavone has the potential value for drug research and development.

Animals ; Anti-Inflammatory Agents, Non-Steroidal ; pharmacology ; Biotransformation ; drug effects ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Flavones ; Flavonoids ; isolation & purification ; pharmacology ; Free Radical Scavengers ; pharmacology ; Ginkgo biloba ; chemistry ; HL-60 Cells ; drug effects ; Humans ; Plants, Medicinal ; chemistry ; Vasodilator Agents ; pharmacology

Objective To investigate whether maternal antibody to hepatitis B surface antigen (anti-HBs)in infants may interfere with the antibody response to hepatitis B vaccine. Methods Infants from singleton pregnant mothers,who delivered at full term at the Affiliated Drum Tower Hospital of Nanjing University Medical School from October 2006 to January 2007,were divided into two groups based on their mothers'status of anti-HBs(43 positive and 29 negative).All infants were vaccinated with hepatitis B vaccine at birth and one month thereafter.Serum anti-HBs were quantitatively determined for the mothers before delivery and for infants in cord blood at delivery and in serum at the age of 1 and 3.5 months. Results Anti-HBs of all 43 newborns in the positive group were positive in cord blood with the coefficiency of 0.98 to the maternal serum anti-HBs level(t=39.05,P<0.01).Forty-two out of the 43 infants remained anti-HBs positive at the age of 1 month.Anti-HBs was negative both at birth and 1 month old in infants of the negative group.However,all infants in both groups were anti-HBs positive at 3.5 months of age,while the average concentration of anti-HBs in infants of the negative group was significantly higher than that of the positive group [(466.9±86.7)mIU/ml vs(151.2±23.1)mIU/ml,t=2.72,P=0.011].Among the 5 infants whose maternal anti-HBs level>1000 mIU/ml,3 did not produce active antibodies against two doses of hepatitis B vaccination. Conclusions Passively acquired maternal anti-HBs in infants can inhibit the active antibody response to hepatitis B vaccine,and the extent of this effect is associated with maternal anti-HBs level.

Objective To explore the relationship between methylenetetra hydrofolate reduetase (MTHFR) C677T genotypo and unstable angina pectoris(UA) in Chinese population. Methods The study consisted of 90 UA cases (UA group), and an age- and sex- matched healthy control cases (control group, n = 90). PC R-RFLP was used to analyze polymorphism of the MTHFR C677T genotypo. The relationship between MTHFR C677T genotype and UA was observed. Results MTHFR 677C→T mutation was found in 30 of 90 patients with unstable angina pectoris (33.33%) and in 15 of 90 control subjects (16.67%). This difference was statistically significant (P<0.05). Conclusion MTHFR 677C→T mutation is closely related to the unstable angina poctoris.

The change of yield and contents. of active compositions were studied while the fibrous roots were decayed naturally. HPLC method was used to detect the contents of active composition. The results show that fibrousroots could decrease the production of plant by 38.60% (20 g) and 30.99% (40 g), respectively. Treatment 1 could increase the contents of dihydrotanshinone and cryptotanshinone of Salvia miltiorrhiza f. alba by 26.08% and 22.64%, respectively. Compared with the comparison, treatment 2 decreased the contents of ihydrotanshinone, cryptotanshinone, tanshinone I and tanshinone II(A) of S. miltiorrhiza f. alba by 60.87%, 79.24%, 84.61% and 88.99%, respectively. Meanwhile, the total contents of the liposoluble constituents reduced by 86.27%. The different concentration of fibrousroots could increase the content of salvianolic acid B by 4.98% (20 g) and 23.64% (40 g), respectively. Meanwhile, the content of rosemary acid was increased by 4.98% (20 g) and 23.64% (40 g), respectively. The content of water-soluble constituents positively correlated to the mount of added fibrousroots, and the change was significantly. The result indicted that the decay of fibrousroots has a significant impact on the growth and the content of the active composition of S. miltiorrhiza f. alba under the condition of continuous cropping. Fibrousroots could decrease the content of biomass and liposoluble constituents significantly, which maybe one of the main factors to S. miltiorrhiza f. alba continuous cropping obstacle formation.
Benzofurans ; analysis ; Biomass ; Chromatography, High Pressure Liquid ; Diterpenes, Abietane ; analysis ; Plant Roots ; metabolism ; Salvia miltiorrhiza ; chemistry ; growth & development

Base Sequence ; Cell Line, Tumor ; Enhancer Elements, Genetic ; genetics ; HT29 Cells ; Humans ; Luciferases ; genetics ; metabolism ; Molecular Sequence Data ; Plasmids ; genetics ; Polymerase Chain Reaction ; Promoter Regions, Genetic ; genetics ; Recombinant Fusion Proteins ; genetics ; metabolism ; Simian virus 40 ; genetics ; Telomerase ; genetics ; Transcription, Genetic ; Transfection

Actins ; metabolism ; Adult ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Breast Neoplasms ; drug therapy ; metabolism ; pathology ; surgery ; Calcium-Binding Proteins ; metabolism ; Carcinoma ; metabolism ; pathology ; Cyclophosphamide ; therapeutic use ; Desmin ; metabolism ; Diagnosis, Differential ; Female ; Fibrosarcoma ; metabolism ; pathology ; Fluorouracil ; therapeutic use ; Follow-Up Studies ; Humans ; Leiomyosarcoma ; drug therapy ; metabolism ; pathology ; surgery ; Mastectomy, Segmental ; Methotrexate ; therapeutic use ; Microfilament Proteins ; metabolism ; Neurilemmoma ; metabolism ; pathology ; Phyllodes Tumor ; metabolism ; pathology ; Postoperative Period ; Vimentin ; metabolism

OBJECTIVETo understand the prevalence of sub-clinical infection of hepatitis E virus (HEV) among blood donors.

METHODSA cluster sampling strategy was used to sample all blood donors from July to August in 2002 in Beijing. Their blood was tested for IgM and IgG antibody against HEV.

RESULTSThe prevalence of anti-HEV IgM among blood donors in Beijing was 1.74%. The rate of abnormal alanine aminotransferase (ALT) in anti-HEV IgM positive donors is significantly higher than anti-HEV IgM negative donors. Among all ALT abnormal donors, 2.68% can be associated with HEV sub-clinical infection. The percentage is similar with HBV but higher than HCV.

CONCLUSIONThere are sub-clinical infection of HEV among blood donors, which is one of the cause of abnormal ALT in the donors.

Adult ; Alanine Transaminase ; blood ; Blood Donors ; Female ; Hepacivirus ; immunology ; Hepatitis Antibodies ; blood ; Hepatitis E ; epidemiology ; Hepatitis E virus ; immunology ; Humans ; Immunoglobulin M ; blood ; Male

OBJECTIVETo investigate the inhibition of Streptococcus oligofermentans (So) on Streptococcus mutans (Sm) and the producibility of hydrogen peroxide by So under the influence of glucose concentration environment.

METHODSThe inhibition between So and Sm was observed by plating method under the different glucose concentration environment. The initial synthesis rates and production of hydrogen peroxide by So were determined under the different glucose concentration environment by 4-aminoantipyine-horseradish peroxidase method at A(510).

RESULTSUnder 0, 10 and 50 mmol/L glucose environment, the inhibition of So on Sm was evident. When both Sm and So were inoculated at the same time, the ratio of inhibition area by bacterial membrane area was 0.202 ± 0.005, 0.467 ± 0.025, 0.468 ± 0.028 under 0, 10, 50 mmol/L glucose environment. When So was cultivated first and then Sm applied, the ratio was 0.394 ± 0.004, 0.811 ± 0.075 and 0.816 ± 0.007 under 0, 10 and 50 mmol/L glucose environment respectively. The inhibition under 10 and 50 mmol/L glucose environment were more significant than that under non-glucose environment. There was no significant difference between these two glucose concentrations (P > 0.05). The initial synthesis rates of H2O2 by So under the 10 mmol/L [(23.573 ± 0.263) µmo×L(-1)×min(-1)] and 50 mmol/L [(23.337 ± 0.473) µmol×L(-1)×min(-1)] glucose were higher than without glucose[(10.513 ± 0.516) µmol×L(-1)×min(-1)], P < 0.05. H2O2 was not detected in 1000 mmol/L glucose. However, the production of H2O2 by So under 0 mmol/L glucose was higher than other glucose concentrations (P < 0.05).

CONCLUSIONSThe capability of the inhibition of So on Sm was affected by glucose environment and was much stronger under certain glucose concentrations (10, 50 mmol/L).

Antibiosis ; Dose-Response Relationship, Drug ; Glucose ; metabolism ; Hydrogen Peroxide ; metabolism ; Streptococcus ; growth & development ; metabolism ; physiology ; Streptococcus mutans ; growth & development ; metabolism

OBJECTIVE(1) To investigate the promoter methylation status of gene p16(INK4a) and gene RB in breast carcinoma and the adjacent non-neoplastic hyperplastic epithelial tissue. (2) To study the correlation of p16(INK4a) gene expression at protein level with the abnormal gene methylation, the clinical manifestation and the pathological parameters.

METHODSMethylation status of promoters of p16(INK4a) gene and RB gene was detected by using methylation specific PCR in 46 cases of breast cancer, 22 cases of the adjacent non-neoplastic hyperplastic epithelium tissue and 7 cases of normal breast tissue. In addition, the p16(INK4a) gene protein expression level was also detected using immunohistochemical technique(SP method) in 46 cases of breast cancer and 22 cases of the adjacent hyperplastic epithelial tissue.

RESULTSThe methylation rate of p16(INK4a) gene was 23.9% (11/46) in breast cancer, 18.2% (4/22) in the adjacent non-neoplastic hyperplastic epithelial tissue and 1/7 in normal breast tissue, respectively. The methylation rate of RB gene was relatively low, which was 10.8% (5/46), 9.1% (2/22) and 0(0/7) in the above 3 groups, respectively. Methylation rate of p16(INK4a) gene and RB gene was not significantly different among the breast cancer, the adjacent non-neoplastic hyperplastic tissue and the normal tissues (P > 0.05). However, the methylation status of p16(INK4a) gene was closely correlated with its protein expression level and the negative ER expression result of the breast cancer (P < 0.05), but not correlated with the size of the cancer, differentiation status, lymph node metastasis, and age. The methylation status of RB gene was correlated with lymph node metastasis, but not with the size, the differentiation status, ER expression of the breast cancer and the age of the patients.

CONCLUSIONSThe abnormal methylation of p16(INK4a) gene may not play a significant role in the early stage of breast cancinogenesis, but may play a role of in the progression of the cancer. RB gene methylation may also be a indicator in choice to identify the progression and prognosis of breast cancer.

Adult ; Aged ; Breast Neoplasms ; genetics ; metabolism ; pathology ; Carcinoma, Ductal, Breast ; genetics ; metabolism ; pathology ; Carcinoma, Intraductal, Noninfiltrating ; genetics ; metabolism ; pathology ; Cyclin-Dependent Kinase Inhibitor p16 ; genetics ; metabolism ; DNA Methylation ; Female ; Gene Expression Regulation, Neoplastic ; Genes, p16 ; Humans ; Lymphatic Metastasis ; Middle Aged ; Receptors, Estrogen ; metabolism ; Retinoblastoma Protein ; genetics ; metabolism