BACKGROUND:The design of post and core of the molar is affected by various factors,including the effect of the number of posts on the retention force and the effect of the number of posts on the dentin stress and distribution.OBJECTIVE:To make a mechanical analysis on the three-dimensional finite element models of the mandibular first molar defect restored with different numbers and locations of gold alloy posts,cores and all-ceramic crown.METHODS:Cone-beam CT based three-dimensional finite element models of post and core restored first mandibular molars were established,with seven different post designs.According to the numbers and location of gold alloy post inserted into root canal,a single post was placed at the mesial buccal root in group A,a single post at the mesial tongue root in group B,a single post at the distal root in group C,two posts at the mesial buccal root and the distal root in group D,two posts at the mesial buccal root and mesial tongue root in group E,two posts at the mesial tongue root and the distal root in group F,one post respectively at the mesial buccal root,mesial tongue root and distal root in group G.The von Mises criterion was applied for comparing the maximum yon Mises stress value of dentin and stress concentration areas in the seven models which restored with golden alloy posts and cores with all-ceramic crown under the maximum (600 N),vertical (225 N),inclined (225 N) and horizontal (225 N) loads.RESULTS AND CONCLUSION:Under vertical,inclined and horizontal direct loads,the maximum stresses on the remaining dentin in group C were significantly lowest,compared with the groups A and B.The maximum stresses on the remaining dentin changed irregularly with the variation of the numbers and location of posts,when the mandibular first molar defect was restored with all-ceramic crown with gold alloy posts and cores.Under the horizontal load,the maximum Von Mises stress value of the dentin increased remarkably.Under the horizontal load,the maximum stresses on the remaining dentin in group E were the highest.To conclude,the numbers and location of posts and cores have influence on magnitude and distribution of stress,when the mandibular first molar defect is restored with all-ceramic crown with gold alloy post and core.

BACKGROUND:The location of residual dentin may affect stress distribution of tooth root. ＜br> OBJECTIVE:To make a three-dimensional finite element analysis of gold al oy post-core and al-ceramic crown in the repair of structure defects of the mandibular first molar, and to provide a guideline for planning restoration for such kind of tooth structure defects. ＜br> METHODS:Three-dimensional finite element models of the restored first mandibular molars were constructed by CT image reconstruction technique. Then MIMICS software was used to separate the areas and finish the three-dimensional calculation. GEOMAGIC software was also applied to modify and generate a NURBS surface in each patch. Al components of the models were assembled under the ANSYS preprocessor. The Mohr strength theory was applied for comparing the maximum Mohr stress value of dentin and stress concentration areas in six models under different simulating loads. ＜br> RESULTS AND CONCLUSION:The restoration effects in six models were compared, the maximum Mohr stress value of dentin and stress concentration areas were not obviously different. The location of residual dentin has little influence on the stress value and distribution, when restored by gold al oy post-core and and al-ceramic crown.

Objective To summarize the clinical experience of urethral realignment for treating early urethral injury under the guidance of ureteroscope,and evaluate its curative effect.Methods Twenty-nine male patients with urethral injury were selected,and 12 patients of posterior urethral injury,17 patients of former urethral injury.All the patients were treated with urethral realignment under the guidance of ureteroscope,postoperative indwelling catheter 3-8 weeks,every 7-16 days changed diameter increase 2 F the catheter 1 time.Results The 29 patients with urethral injury were a indwelling catheter success,all patients were no incontinence after operation 3 months.In the 29 patients,27 patients were urination unobstructed after catheter removal,2 patients were appeared urine line slim after 2 weeks,the 2 patients were normal urination after short urethral expansion.Conclusions The urethral realignment for treating early urethral injury under the guidance of ureteroscope has simple,lower complication,rapid recovery,better effect.The continuous flexible progressive urethral expansion and the strict nursing,which can effectively reduce the occurrence of urethral stricture.

Objective To explore the application of the ultrasonic cardiac output monitor(USCOM) in the children with pneumonia complicated with heart failure.Methods Fourteen children with pneumonia complicated with heart failure were enrolled in the experimental group and thirty-two children with common pneumonia were enrolled in the control group.We used the USCOM to measure the cardiac output indicators,including the aortic peak velocity,heart rate,stroke volume index,cardiac index,ejection time and correcting ejection time.Results The heart rate in the experimental group was faster than that of control group[(174 ±10) beats/min vs (133 ± 14) beats/min,P ＜0.05],the aortic peak velocity in the experimental group was lower than that of control group [(1.246 ±0.234) m/s vs (1.449 ±0.300) m/s,P ＜0.05],the stroke volume index in the experimental group was lower than that of control group [(29.357 ± 6.500) ml/m2 vs (40.188 ±5.337) ml/m2,P ＜0.05],and the correcting ejection time in the experimental group was shorter than that of control group [(342.560 ± 8.219) ms vs (354.430 ± 16.500) ms,P ＜ 0.05].Conclusion The cardiac function monitoring by USCOM is accurate and convenient,and USCOM can provide the accurate evidence for the clinical application.

This study was aimed to investigate effect on the expression of cell inflammatory factor IL-1α, IL-1β and IL-6 of modified San-Jia-San (SJS) decoction on cultured hippocampal neurons injury induced by β-amyloid 25-35 protein (Aβ25-35). Primary cultured hippocampal neurons were divided into the normal group, model group, huperzine A group, low-dose SJS group, middle-dose SJS group, and high-dose SJS group. After selective culture for 7~10 days and the absorption of culture fluid, the blank culture fluid, normal cerebrospinal fluid (normal saline group), huperzine A cerebrospinal fluid, and low-, middle- and high-dose SJS cerebrospinal fluid were added, respectively. The culture fluid was added up to an equivalent medium. The incubation was 2 h under the temperature of 37℃. Ex-cept the normal group, Aβ25-35 dealt with aging (the final concentration was 5 μmol/L) was added to other groups to establish AD cell model. An equivalent amount of culture fluid was added to the normal group. After 24 h of incuba-tion, the cell culture supernatant was collected. Enzyme-linked immunosorbent assay (ELISA) was used in the content detection of IL-1α, IL-1β and IL-6. The results showed that modified SJS decoction can decrease the content of IL-1α, IL-1β and IL-6 in the cell culture supernatant. It was concluded that the modified SJS decoction can effectively inhibit the expression of cell inflammatory factor IL-1α, IL-1β and IL-6. It had certain anti-inflammatory effect to protect hippocampal neurons.

Objective To investigate the effects and mechanisms of sulindac, a non-selective cyclooxygenase(COX)inhibitor, on the proliferation and apoptosis of colorectal cancer cell line HT-29.Methods HT-29 cells were treated with sulindac. MTT assay and flow cytometry were used to measure the proliferation and apoptosis respectively. The laser scanning microscope(LSM)and the fluorescence microscope were used to observe apoptosis of the cells, and the flow cytometry (FCM)analysis was used to observe the cell apoptosis and cell cycle. Results Sulindac inhibited the cells proliferation and induced apoptosis in a dose-and time-dependent manner. With the TUNEL staining and fluorescence microscope, we found that the apoptosis cell became brown. After the Annexin V/PI staining, we observed that the membrane of apoptosis cells became green with LSM; the nucleosidase became red or crocus. FCM showed that sulindac promoted apoptosis of the cells, made the stage of G0/G1 ceils significantly reduced. Conclusions Our results showed that sulindac may inhibit the proliferation and induced the apoptosis of colon cancer cell HT-29,and the mechanism may probably be related to cell cycle arrest.

Animals ; Base Sequence ; Gene Expression Regulation ; Humans ; MicroRNAs ; genetics ; metabolism ; Molecular Sequence Data ; RNA, Viral ; genetics ; Virus Diseases ; genetics ; metabolism ; virology ; Virus Physiological Phenomena ; Viruses ; genetics ; metabolism

Objective To investigate the carboprost ammonia butyl alcohol three plus motherwort injection on prevention of high risk maternal postpartum hemorrhage effect .Methods 120 cases of cesarean section operation and potential bleeding symptom of women were selected as the research object ,and randomly divided into two groups , the observation group of 60 cases using carboprost ammonia butyl alcohol three joint of leonurus heterophyllus injec -tion for prevention and treatment ,the control group of 60 cases used oxytocin for prevention and treatment ,prevention and treatment effects were compared between the two groups .Results After treatment,the observation group 2h,24h postpartum hemorrhage were (422.41 ±213.49)mL,(35.29 ±16.44)mL,were significantly less than those in the control group (589.64 ±345.21)mL,(69.31 ±29.47)mL(t=6.732,8.915,all P<0.05);the observation group 1 cases without production after bleeding ,control group 5 cases of postpartum hemorrhage ,statistically significant differ-ences between the two groups (χ2 =4.973,P<0.05).Conclusion Carboprost ammonia butyl alcohol three plus motherwort injection on prevention of high risk maternal postpartum hemorrhage has a significant effect ,which can re-duce the cesarean section uterine bleeding and reduce the amount of bleeding ,promote uterine involution ,conducive to maternal rehabilitation .

Coronary Artery Disease ; pathology ; Humans ; Medicine, Chinese Traditional

AIM:To investigate the expression of poly(ADP-ribose) polymerase-2 (PARP-2) during rat car-diac hypertrophy in vitro and in vivo, and to explore the effects of PARP-2 on the cardiac hypertrophy.METHODS:Ab-dominal aortic coarctation ( AAC) was performed to establish a model of pressure overload-induced cardiac hypertrophy in SD rats.The expression of PARP-2 at mRNA and protein levels in the myocardium was determined by real-time PCR and Western blot.The hypertrophy model of the cardiomyocytes was induced by treating the cells with angiotensinⅡ( AngⅡ) . PARP-2 was knocked down by siRNAs in neonatal rat cardiomyocytes and the cardiomyocyte hypertrophy was evaluated by measuring the mRNA levels of ANF, BNP, and β-MHC and the cellular surface area.RESULTS: The expression of PARP-2 at mRNA and protein levels was both increased in the AAC rats as compared with those in the sham animals.The expression of PARP-2 at mRNA and protein levels was also increased in a time-and concentration-dependent manner in AngⅡ-induced hypertrophy model of the cardiomyocytes.In the neonatal rat cardiomyocytes, knockdown of PARP-2 ex-pression by siRNA attenuated AngⅡ-induced cardiac hypertrophy of the cardiomyocytes, indicating that endogenous PARP-2 played a positive regulatory role in cardiac hypertrophy.CONCLUSION:The mRNA and protein levels of PARP-2 in-crease in the in vitro and in vivo models of cardiac hypertrophy.Knockdown of PARP-2 protects cardiomyocytes from hyper-trophy.