We found that the number of institutions made use of the undocumented in vitro diagnostic reagent in the survey. The phenomenon poses some risks and problems. In use this paper, we analyzed the situation and the reasons for the use of the undocumented in vitro diagnostic reagents, and put forward the corresponding measures.
Humans ; Indicators and Reagents ; standards

Acetates ; urine ; Chromatography, Gas ; Gas Chromatography-Mass Spectrometry ; Humans

Based on the requirement of biology education development,the article analyzes the approach to improve the teaching quality of medical biology from quality education,subjiect intercrossing or 'regression' and 'outspread',and offers new ideas for education reform and innovation of the medical biology.

In recent years, various studies and analyses related to cosmetics safety were conducted according to routine tests in Hygienic Standard of Cosmetics (2007), and the eligible rates of tested cosmetics were high. But the other prohibited and limited use components, such as antibiotics were analyzed rarely, and meanwhile some kinds of cosmetic related dermatitis cases appeared dramatically. Several dermatitis, especially contact dermatitis and hormone dependent dermatitis symptoms were not contained in Diagnostic Criteria and Principles of Management of Skin Diseases Induced by Cosmetics-General Guideline, GB 17149.1—1997. So it indicated the standard, GB 17149.1—1997 should be revised and some prohibited and limited use components such as hormone and antibiotic testing should be appended to the safety analysis of cosmetics.

BACKGROUND: Many studies focus on transforming growth factor β (TGF β) and its receptors, however, the distdbution of type Ⅰ TGF receptor (TGF-βR Ⅰ) in peripheral region of hypertrophic scars remain poorly understood. OBJECTIVE: To determine the expression and distribution of TGF-βR Ⅰ and type Ⅰ collagen in the peripheral and central areas of human skin hypertrophic scar. METHODS: A total of 30 cases with human cutaneous scars admitted at the Department of Plastic Surgery, First Affiliated Hospital and Department of Mammary Gland, Head and Neck Surgery, Tumor Hospital of Xinjiang Medical University from 1999 to 2002, were selected, including 20 cases with hypertrophic scar and 10 cases with normal scars. A total of 180 scars were obtained from central and peripheral areas of scars as well as normal skin tissues. The protein contents of TGF-βR1 and type Ⅰcollagen was detected by immunohistochemistry. In addition, the immunostaining positive in these samples was analyzed by semiquantitative analysis. RESULTS AND CONCLUSION: Compared to non hypertrophic scar and normal skin tissues, the TGF-βR1 expression of hypertrophic scar was obvious greater with strong positive reaction. The TGF-β R Ⅰ content was 100% in peripheral region of hypertrophic scar, which was notably 20% greater than that of central area (P < 0.05). The content of type Ⅰ collagen was both 100% in peripheral and central areas. The differences of positive TGF-β R Ⅰ and type Ⅰ collagen had no significance between peripheral and central areas of non hypertrophic scars (P > 0.05). There were few contents of TGF-βR Ⅰ and type Ⅰ collagen in normal skin tissues. The expression of TGF-β R Ⅰ is higher in peripheral than central areas of hypertrophic scar. Therefore, the peripheral area would be emphasized in the clinic work.

Churg-Strauss Syndrome ; complications ; Coronary Artery Disease ; complications ; Humans ; Male ; Middle Aged

Accidents, Occupational ; prevention & control ; Occupational Diseases ; prevention & control ; Occupational Health Services ; organization & administration ; Risk Assessment ; methods ; organization & administration

Objective To prepare an active anti-tumor component, compound K (C-K), from saponins in leaves of Panax notoginseng (SLPN) using immobilized β-glucanase. Methods Two entrapments, alginate gel-1 (Alg 1) and alginate gel-2 (Alg 2), were evaluated for their ability to immobilize β-glucanase. The amount and purity of C-K obtained from the transformation process were analyzed by HPLC, and the immobilizing parameters were optimized. Results β-Glucanase can be immobilized and reused with either of the entrapment. However, using AIg 1 resulted in higher enzyme activity than Alg 2. The optimal concentration of the immobilized enzyme was 10%; The optimal crosslinking time was 4-6 h; and the optimal concentration of the crosslinking agent was 6%-7%. Conclusion Immobilized β-glucanase shows sustained enzyme activity, good ethanol tolerance, and was reusable for the preparation of C-K from SLPN.

The silent cerebral infarct is an clinic symptom that is so slight or transitory as to be easily neglected. There are only neural symptoms and signs,but irresponsible infarct focus can be seen on image. The risk factors of silent cerebral infarct may be the same as those of symptomatic cerebral infarct. Such infarct is likely to influence the course, clinic symptom and prognosis of acute cerebral infarct and may foretell symptomatic cerebral infarct and deteriorate into pseudo-global paralysis or multi-infarcted dementia. Therefore elder who suffer from hypertension or diabetes and experience vertigo, headache, language barrier but without apparent signs and symptoms should receive cerebral CT or MRI. Minor or third-degree precautionary measures can be taken as a chief therapy for cerebral infarct. Alternatively vitamines B_6,B_(12) and folic acid can be supplied to reduce the chance of hyperhomocysteinemia. Headache is the initial symptom in silent brain infarct. Magnesium is effective when dehydration does not work.

Objective To discuss the role of endothelicyte activation in the development of pulmonary hypertension (PH) and vascular remodeling. Methods A total of 10 patients with simple ventricular septal defect(VSD)of congenital heart disease(CHD)were choosen as controls, and 30 patients with different degree of PH were studied. Plasma levels of interleukin-1(IL-1), endothelin-1 (ET-1) and nitric oxide (NO) which represented the degree of endothelicyte activation were measured by radioimmunoassay and commercial available kit. The specimens taken from right lung before cardiopulmonary bypass were fixed by formaldehyde solution. The percentage of vascular wall thickness/vascular external diameter (WT/D) and the percentage of vascular area/total vascular area (WA/A) which represented vascular remodeling, were measured by QTM970 analysator. Results Compared with patients of simple VSD, the plasma levels of IL-1, ET-1/NO and WA/A, WT/D increased in patients with PH. Conclusion The activation of endothelicytes and imbalance of ET/NO play an important role in the development of PH and vascular remodeling. Positive correlations were seen between IL-1, ET-1/NO and WA/A, WT/D(P