1.Spatial and temporal expression of c-mos in mouse testis during postnatal development.
Shao-Feng CAO ; Ding LI ; Qing YUAN ; Xin GUAN ; Chen XU
Asian Journal of Andrology 2008;10(2):277-285
AIMTo immunolocalize the c-mos gene product and to investigate its spatial and temporal expression in mouse testis during postnatal development.
METHODSSemi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization techniques were used to examine c-mos mRNA and indirect immunofluorescence was used to localize c-Mos protein in mouse testis on postnatal days 14, 21, 25, 28, 30, 35, 49 and 70.
RESULTSc-mos mRNA remained low on postnatal days 14-21, increased abruptly from day 25 and peaked on day 30. Its levels decreased a little on day 35 and became almost stable thereafter until day 70. c-mos mRNA was localized in the nucleus and cytoplasm of the spermatocytes and round spermatids. The nuclear staining was much stronger than the cytoplasmic staining. Using a polyclonal anti-c-Mos antibody, Western blotting detected a single band at 43 kDa in testis lysate. c-Mos protein was exclusively localized to the elongating spermatids and was first detected on postnatal day 30. The number of c-Mos-positive spermatids increased progressively till day 49 and stabilized thereafter.
CONCLUSIONThe c-mos gene displays a spatial and temporal expression pattern in the mouse testis during postnatal development at both the mRNA and protein level. This suggests that c-mos might play important roles in spermatogenesis.
Animals ; Fluorescent Antibody Technique, Indirect ; Gene Expression ; Genes, mos ; genetics ; Male ; Mice ; Reverse Transcriptase Polymerase Chain Reaction ; Spermatocytes ; growth & development ; metabolism ; Spermatogenesis ; genetics ; Testis ; metabolism
2.Risk Factors for Metachronous Gastric Neoplasms in Patients Who Underwent Endoscopic Resection of a Gastric Neoplasm.
Hyuk YOON ; Nayoung KIM ; Cheol Min SHIN ; Hye Seung LEE ; Bo Kyoung KIM ; Gyeong Hoon KANG ; Jung Mogg KIM ; Joo Sung KIM ; Dong Ho LEE ; Hyun Chae JUNG
Gut and Liver 2016;10(2):228-236
BACKGROUND/AIMS: To identify the risk factors for metachronous gastric neoplasms in patients who underwent an endoscopic resection of a gastric neoplasm. METHODS: We prospectively collected clinicopathologic data and measured the methylation levels of HAND1, THBD, APC, and MOS in the gastric mucosa by methylation-specific real-time polymerase chain reaction in patients who underwent endoscopic resection of gastric neoplasms. RESULTS: A total of 257 patients with gastric neoplasms (113 low-grade dysplasias, 25 high-grade dysplasias, and 119 early gastric cancers) were enrolled. Metachronous gastric neoplasm developed in 7.4% of patients during a mean follow-up of 52 months. The 5-year cumulative incidence of metachronous gastric neoplasm was 4.8%. Multivariate analysis showed that moderate/severe corpus intestinal metaplasia and family history of gastric cancer were independent risk factors for metachronous gastric neoplasm development; the hazard ratios were 4.12 (95% confidence interval [CI], 1.23 to 13.87; p=0.022) and 3.52 (95% CI, 1.09 to 11.40; p=0.036), respectively. The methylation level of MOS was significantly elevated in patients with metachronous gastric neoplasms compared age- and sex-matched patients without metachronous gastric neoplasms (p=0.020). CONCLUSIONS: In patients who underwent endoscopic resection of gastric neoplasms, moderate/severe corpus intestinal metaplasia and a family history of gastric cancer were independent risk factors for metachronous gastric neoplasm, and MOS was significantly hypermethylated in patients with metachronous gastric neoplasms.
Aged
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Basic Helix-Loop-Helix Transcription Factors/genetics
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DNA Methylation
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Female
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Gastrectomy/methods
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Genes, APC/physiology
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Genes, mos/genetics
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Humans
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Incidence
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Male
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Middle Aged
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Multivariate Analysis
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Neoplasms, Second Primary/epidemiology/*genetics/pathology
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Proportional Hazards Models
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Risk Factors
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Stomach Neoplasms/genetics/*pathology/surgery
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Thrombomodulin/genetics