1.C-reactive protein as an indicator of inflammatory responses to experimentally induced cystitis in dogs.
Kyoung Won SEO ; Jong Bok LEE ; Jin Ok AHN ; Hee Woo LEE ; Cheol Yong HWANG ; Hwa Young YOUN ; Chang Woo LEE
Journal of Veterinary Science 2012;13(2):179-185
The aim of this study was to demonstrate and assess C-reactive protein (CRP) changes in dogs with induced bacterial cystitis with or without antibiotics. We also evaluated availability of CRP levels to serve as an indicator for monitoring or diagnosing bacterial cystitis. Serial CRP concentrations in dogs with induced bacterial cystitis were higher than those of controls (p < 0.001). CRP concentrations peaked on day 7 and gradually decreased thereafter. In the treatment group, CRP concentrations decreased after medication compared to the untreated group (p = 0.032). CRP levels had a linear correlation with urine white blood cell counts among all groups (r = 0.837, p < 0.001, n = 140). Compared to the negative urine culture group, dogs with positive urine culture results had higher CRP concentrations (median 43.8 mg/L vs. 5.9 mg/L; p < 0.001). Area under the receiver operating characteristic curve was 0.955; when cut-off value was 12.2 mg/L, CRP measurements were found to have a sensitivity of 92.3% and specificity of 86.4%. This result indicates that rapid increases of CRP occurred after inducing bacterial cystitis and CRP may be a useful indicator for monitoring or diagnosing canine bacterial cystitis together with sediment urinalysis and urine bacterial culture.
Amoxicillin-Potassium Clavulanate Combination/therapeutic use
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Animals
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Anti-Bacterial Agents/therapeutic use
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C-Reactive Protein/genetics/*metabolism
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Cystitis/metabolism/*veterinary
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Dogs
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Gene Expression Regulation/*physiology
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Inflammation/*metabolism
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Male
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Proteus Infections/drug therapy/metabolism/microbiology/*veterinary
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Proteus mirabilis
2.In vitro and in vivo gene therapy with CMV vector-mediated presumed dog beta-nerve growth factor in pyridoxine-induced neuropathy dogs.
Jin Young CHUNG ; Jung Hoon CHOI ; Il Seob SHIN ; Eun Wha CHOI ; Cheol Yong HWANG ; Sang Koo LEE ; Hwa Young YOUN
Journal of Veterinary Science 2008;9(4):367-373
Due to the therapeutic potential of gene therapy for neuronal injury, many studies of neurotrophic factors, vectors, and animal models have been performed. The presumed dog beta-nerve growth factor (pdbeta-NGF) was generated and cloned and its expression was confirmed in CHO cells. The recombinant pdbeta-NGF protein reacted with a human beta-NGF antibody and showed bioactivity in PC12 cells. The pdbeta-NGF was shown to have similar bioactivity to the dog beta-NGF. The recombinant pdbeta-NGF plasmid was administrated into the intrathecal space in the gene therapy group. Twenty-four hours after the vector inoculation, the gene therapy group and the positive control group were intoxicated with excess pyridoxine for seven days. Each morning throughout the test period, the dogs' body weight was taken and postural reaction assessments were made. Electrophysiological recordings were performed twice, once before the experiment and once after the test period. After the experimental period, histological analysis was performed. Dogs in the gene therapy group had no weight change and were normal in postural reaction assessments. Electrophysiological recordings were also normal for the gene therapy group. Histological analysis showed that neither the axons nor the myelin of the dorsal funiculus of L(4) were severely damaged in the gene therapy group. In addition, the dorsal root ganglia of L(4) and the peripheral nerves (sciatic nerve) did not experience severe degenerative changes in the gene therapy group. This study is the first to show the protective effect of NGF gene therapy in a dog model.
Amino Acid Sequence
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Animals
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Base Sequence
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CHO Cells
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Central Nervous System Diseases/chemically induced/therapy/*veterinary
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Cloning, Molecular
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Cricetinae
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Cricetulus
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Cytomegalovirus
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Dog Diseases/*chemically induced/therapy
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Dogs
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Female
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Gene Therapy/*veterinary
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Genetic Vectors
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Male
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Molecular Sequence Data
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Nerve Growth Factor/genetics/*metabolism/*therapeutic use
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Pyridoxine/*toxicity
3.Evaluation of a side population of canine lymphoma cells using Hoechst 33342 dye.
Myung Chul KIM ; Susan D'COSTA ; Steven SUTER ; Yongbaek KIM
Journal of Veterinary Science 2013;14(4):481-486
Cancer stem cell (CSC) research has increased exponentially to gain further insight into the mechanisms underlying both carcinogenesis and chemotherapy resistance. The present study was performed to explore the potential value of a side population (SP) assay for identifying and characterizing putative CSCs among canine lymphoma cells. Canine lymphoma cells from cell lines and clinical samples were subjected to the SP assay consisting of Hoechst 33342 staining and subsequent flow cytometric analysis. The SP assay revealed various amounts of a SP fraction among the canine lymphoma cells. The percentages of SP were not affected by inhibitors of membrane transporters, verapamil hydrochloride, or fumitremorgin C. Most of the canine lymphoma cells expressed high levels of Bmi-1 and membrane transporter proteins such as ABCG2 and phosphorylated (p)-glycoprotein. This investigation lays the groundwork for further studies of the biological behaviors and molecular characteristics of CSCs in cases of canine lymphoma.
Animals
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Benzimidazoles/*metabolism
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Cell Line, Tumor
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Dog Diseases/*diagnosis/drug therapy/pathology
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Dogs
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Flow Cytometry/*methods/veterinary
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Fluorescent Dyes/*metabolism
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Gene Expression Regulation, Developmental
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Lymphoma/diagnosis/drug therapy/pathology/*veterinary
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Neoplastic Stem Cells/drug effects/*metabolism/pathology
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Side-Population Cells/drug effects/*metabolism/pathology
4.Multidrug-resistant Salmonella typhimurium and Salmonella enteritidis identified by multiplex PCR from animals.
Soo Jin YANG ; Kyong Yoon PARK ; Keun Seok SEO ; Thomas E BESSER ; Han Sang YOO ; Kyoung Min NOH ; So Hyun KIM ; Shuk Ho KIM ; Bok Kwon LEE ; Yoon Ho KOOK ; Yong Ho PARK
Journal of Veterinary Science 2001;2(3):181-188
Antibiotic resistance in Salmonella enteritidis and S. typhimurium, one of most frequent etiologic pathogens of food-borne bacterial gastroenteritidis in humans, is a serious health problem worldwide. Fifteen and 22 each of S. enteritidis and S. typhimurium were isolated from animals from 1983 to 1999 in Korea and tested for their antibiotic resistance patterns and phage types. S. enteritides isolates were highly resistant to sulfonamides (86.7%) and four of them (26.6%) showed multiple antibiotic resistance. The most frequent phage type (PT) of S. enteritids was PT1 (33.3%) even though none of them had multiple antibiotic resistance. S. typhimurium isolates were highly resistant to streptomycin, sulfonamides, and tetracycline, 100%, 95.5%, and 86.4% respectively. The incidence of multiple antibiotic resistance of S. typhimurium isolates was extremely high (100%) comparing to S. enteritidis isolates (26.7%). Two of the five ACSSuT type S. typhimurium isolates, resistant to ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline, were phage type DT104. All S. typhimurium isolates were sensitive to florfenicol. For the rapid detection of multiple antibiotic resistant S. enteritidis and S. typhimurium isolates, particularly ACSSuT type S. typhimurium DT104, antibiotic resistance genes, cmlA/tetR, PSE-1, and TEM, and Salmonella spp. Specific gene, SipB/C, were amplified using four pairs of primers in hot-started multiplex polymerase chain reaction. Two Korean isolates of S. typhimurium DT104 showed TEM amplicons instead of PSE-1 for the ampicillin resistance. The multiplex PCR used in this study was useful in rapid detection of ACSSuT type S. typhimurium and identification of b-lactamase gene distribution among Salmonella isolates.
Animals
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Anti-Bacterial Agents/*pharmacology
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Bacteriophage Typing
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Base Sequence
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Drug Resistance, Bacterial/genetics
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Drug Resistance, Multiple, Bacterial/genetics
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Gene Amplification
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Humans
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Microbial Sensitivity Tests/veterinary
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Phenotype
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Polymerase Chain Reaction/veterinary
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Salmonella Infections, Animal/drug therapy/*microbiology
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Salmonella enteritidis/classification/*drug effects/genetics
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Salmonella typhimurium/classification/*drug effects/genetics