The study of blood samples extracted directly from healthy people, who is Vietnamese and not relative, lives over the country. PCR technique and denaturing polyacrylamid gel electrophoresis were applied for determination of allele frequencies of D13S317. The primary results showed that allele frequencies of D13S317 locus was polymorphic AND, repetitive sequence unit of 4 nito- base which located on the long hand of chromosome No 13. The locus contained 9 alleles, 242-270 bp. The numbers of repeat units in population were 7, 8, 9, 10, 11, 12, 13, 14, 15. The highest frequency at allele No 8, and allele No 7 had not appear in the observed samples.
Population ; Gene Frequency ; analysis

Alleles ; Gene Frequency ; Genotype

Granulocyte-specific antigens, such as NA1, NA2, NBI, NB2, NC1, NDI, NE1, are a group of antigens specifically expresesed only on the granulocytes. Antibodies against these are involved in some clinical disorders such as alloimmune neonatal neutropenia(ANN), autoimmune neutropenia(AIN), and transfusion-related acute lung injury(TRALI). We investigated the frequencies of NA1, NA2, NB1, and Mart antigens among Koreans by the granulocyte indirect immunofluorescence test employing flow cytometry. The subjects were 105 Koreans(male 65, female 40), whose mean age was 31.7+/-8.2 years (range 16~57). The antigen and gene frequencies were as follows, NA1, 0.78, 0.53, NA2, 0.75, 0.50, NB1 0.86, 0.62, and Mart, 1.00, 1.00, respectively. The proportions of NB 1 -positive granulocytes among NB 1-positive individuals were variable(range, 27~100%). Through this study, the authors procured granunlocyte-specifiic antigen papnel, which is essential in the identification of causative antibody(-ies) in immune neutropenias.
Antibodies ; Female ; Flow Cytometry ; Fluorescent Antibody Technique, Indirect ; Gene Frequency ; Granulocytes ; Humans ; Lung ; Neutropenia

Most common diseases are complex genetic traits. Identifying the genes that underlie the variation in the diseases has been challenging. Two major approaches have been used to map genetic variants that influence disease risk: linkage analysis and association study. Linkage analysis tests for the co-segregation of a marker and disease phenotype within a pedigree, whereas association study tests for differences in marker allele frequencies between patients and a control population. Linkage analysis is applied without any prior knowledge of the biological basis of the disease. In association study, genes with a known function with the potential to influence the disease phenotype are investigated for a direct role in disease. No single method is sufficient. A multi-strategy approach to the mapping of complex diseases is required. We review the different types of genetic studies to find genes for complex genetic traits.
Gene Frequency ; Humans ; Pedigree ; Phenotype

Experimental study for extracting DNA and determining HLA of I and II class by PCR-SSO was carried out. The percentage of allele frequency was estimated in comparison with that was reported in 1991 conference. Result showed no difference in locus A and DR12. Locus A demonstrated the highest percentage with some differences of 2-4%. In Locus DR12 with the highest percentage of differences of 3%. Highest difference was estimated in locus B. In this study, B15 has 25.7% (in comparing with 0%). B62 had 0% (in comparing with 10.1%), B75 6.7% (in comparing with 0%), B7 12.2% (in comparing with 0%)
DNA ; HLA Antigens ; Gene Frequency

No abstract available.
Alleles* ; AMP Deaminase ; Gene Frequency

To determine allele frequency of GABARB1 Jcus in Korean, first, we sequenced each allele, which was amplified with primers that published before. We found mismatching between allele number and size of each allele when 'GATA' repeats are counted. The sequence revealed new 'GATA' repeat. So we had made new allele designation with total number of in 176 Korean people. The heterozygosity this locus was 0.716 and mean exclusion chance (MEC) and power of discrimination (PD) were 0.433 and 0.845, respectively. In this study, we emphasized necessity of sequencing of 2-3 alleles, which have same size before setting up allelic ladder.
Alleles* ; Discrimination (Psychology) ; Gene Frequency*

OBJECTIVE: To explore the features of genetic differentiation and gene flow of ten minorities in Yunnan province according to nine CODIS short tandem repeat(STR) loci (CSF1PO, FGA, THO1, TPOX, v WA, D3S1358, D5S818, D7S820 and D13S317). METHODS: Heterozygosity and parameters of population differentiation such as F, theta, f and Gst at each locus were calculated. DA genetic distance and fixation index Fst were calculated by Phylip 3.6 and Arlequin 3.0 software, respectively. Phylogenetic trees were constructed by Mega 3.0, and the patterns of gene flow were analyzed by R-matrix model. RESULTS: It showed that average genetic heterogeneity in ten minorities was above 0.7. Significant difference was found for most of the loci in genetic differentiation. Phylogenetic tree analysis showed that the ten minorities were divided into two clusters. The results of the R-matrix analysis showed that the gene flow of Yi and Dai minorities were higher than that of other minorities, while the pattern of gene flow of Dulong minority demonstrated some of the isolation. CONCLUSION Nine STR loci commonly used in forensic identification show a high polymorphism. Heterozygosity can be used for investigating genetic differentiation and gene flow of minority. The ten minorities in Yunnan are independent populations, while the level of differentiation is not high. The relationship in evolution is not far from each other and shows a widely gene flow among the minorities.
Alleles ; China/ethnology* ; Cluster Analysis ; Ethnicity/genetics* ; Gene Flow ; Gene Frequency ; Genetic Structures ; Genetics, Population ; Genotype ; Humans ; Microsatellite Repeats/genetics* ; Models, Genetic ; Pedigree ; Phylogeny ; Polymorphism, Genetic ; Regression Analysis

Research to build database of Nineplexll allelic frequency of Vietnam population applied for gene forensic purposes. The results as followed: there were 6 alleles in D3S1358; 19 alleles in FGA. In Viet (Kinh) population, polymorphism of vWA, especially of FGA was higher than in some other foreign population. For examples, the number of vWA alleles in Viet population is 9, but in Japanese and Caucasian population is 8; FGA alleles in Viet population is 19, but in Japanese is 15, and in Caucasian is 12. Two alleles of FGA are 14 and 32 were found out. There are many ethnics living in Vietnam, so the authors would like to survey allelic frequency of other ethnics.
Population ; Gene Frequency ; analysis ; Forensic Medicine

No abstract available.
Erythroblastosis, Fetal* ; Gene Frequency* ; Infant, Newborn