To determine whether p53 gene transcripts were altered in thyroid tumor and adjacent tissues of thyroid tumor, total RNA from 12 tumor specimens (9 carcinomas and 3 adenomas) and 4 adjacent tissues of thyroid tumor was exmined by Northern blot hybridization and reverse transcription——polymerase chain reaction. It was found that p53 gene transcripts were present in all tumors and the adjacent tissues of tumor and were significantly more obvious in the samples of carcinomas as compared with those of adenomas and in the samples of tumor as compared with those of the adjacent tissues of tumor. These data suggest that excessive p53 gene expressions are involved in thyroid carcinogenesis.

Objective:To study the role of TOLL-like receptor4/MAPKs pathway of renal tissue in the diabetic rats treated with piogitazone. Methods:The male Sprague Dawlry rats were randomly selected as the control group (n=8) and experimental group(n=32). The experimental rats were randomly divided into three groups. The expression of ERK1/2,JNK and p38MAPK,and levels of their phosphorylation in kidney were measured by Western blot in control group and experimental group. Results:The expression of TOLL-like receptor 4 in all renal tissue in the diabetic rats were significantlyincreased than those in control group (P<0. 01). Compared with the control group. The activity of p-ERK1/2 and p38MAPK significantly increased in each experimental group (P<0. 05),but the expression level of p-JNK was no statistical significance. TOLL-like receptor 4 could regulatory effect on the phorylation of ERK1/2 and p38MAPK. Compared with the model group,the expression of p-ERK1/2 and p38MAPK significantly decreased(P<0. 05),moreover, these changes were more significant in high-dose treated group ( P<0. 05 ) . Conclusion:Pioglitazone inhibits the expression of TOLL-like receptor 4 of renal tissue in the diabetic rats,and these effects may be related to TOLL-like receptor 4/ERK1/2 and TOLL-like receptor 4/p38MAPK pathways.

PURPOSE To investigate the methylation of p53 gene in human thyroid carcinoma. METHODS The DNA of 12 thyroid carcinomas and 5 adjacent tissues of thyroid carcinoma were digested by restriction endonuclease enzymes Hap I and Msp I . Polymerase chain reaction (PCR) followed by agarose gel electrophoresis was used to detect the methylation in the exon 5 of p53 gene. RESULTS 5'-CCGG-3' site was methylated at the exon 5 of p53 gene in 9 thyroid carcinomas. However hypomethylation was found to exist in 5 adjacent tissues of thyroid carcinoma. CONCLUSION Hypermethylation of p53 gene plays an important role in thyroid carcinogenesis and the mutation of p53 gene.

Objective To investigate the effect of serum-deprivation on the changes of [ Ca2+ ] i and the protein release of S100A13 and fibroblast growth factor 1 ( FGF-1 ) in thyroid cancer TT cell,and to reveal the role of Ca2+in the protein release of S100Al3 and FGF-1.Methods The protein expressions of FGF-1 and S100A13 in TT cells under serum-deprivation were detected by Western blot.The released FGF-1 protein from TT cells in the supernatant fluid was detected by ELISA.Realtime dynamic examinations on the change of 1 h [ Ca2+ ] i in TT cells under serum-deprivation were detected by confocal laser scanning microscopy.Then,the effect of EGTA( 2.5 mmol/ L),BAPTA-AM (2.5 μmol/L)on distributions of the fluorescence of S100AI 3 and FGF-1 in TT cells under serumdeprivation for6 h were detected by indirect immunofluorescence.Results The expressions of FGF-1 and S100A13 in TT cells after serum-deprivation for4 h and 6 h were reduced( P＜0.05 or P＜0.01 ),but the released FGF-1 protein from TT cells in the supernatant fluid was elevated ( P＜0.05 or P＜0.01).Confocal laser scanning of Ca2+ imaging indicated that [ Ca2+ ] i of serum-deprivation TT cells maintained the relative stabilization within 23 win,but the rapid rise of [ Ca2+ ] i achieved peak value 1.6 μmol/L after 30 min,and remained stable for about 17 win,and thereafter 40 win slowly dropped to a low level From 40 win to 60 win the [ Ca2+ ] i was about 0.3-0.6 μ mol/L.The average [ Ca2+ ] i was higher than that in normal group,EGTA group,and BAPTA-AM group within 1 h.The protein expressions of S100A13 and FGF-1 did not drop obviously in EGTA group and BAPTA-AM group.Conlusion The release of S100A13 and FGF-1 from TT cell under serum-deprivation is possibly related with the change of [ Ca2+ ]i.Both Ca2+-chelating agents EGTA and BA PTA-AM are able to inhibit the rise of [ Ca2+ ] i and release of S100A 13 and FGF-1 from TT cells under serum-deprivation.

Zinc(Zn) and copper(Cu) balance tests were conducted in patients with diabetes mellitus. The results revealed that the negative balance of Zn and excessive positive balance of Cu were much improved after the management of diabetes mellitus. In addition, a close relationship was found between serum glucose concentration and the disorder of Zn and Cu balance, i.e. serum Zn level, apparent intestinal absorption rate and balance status of Zn were reversely related to serum glucose concentration, whereas urinary Zn, serum Cu level and apparent intestinal absorption of Cu were positively correlated to serum glucose concentration. Poly-factor analysis suggested that hyperzincuria and malabsorption of Zn were the main factors concerning negative Zn balance.

Objective:To explore the mechanism of islet cell apoptosis caused by glucose at different concentrations.Methods:The islet cells of SD adult rats were prepared by collagenase digestion,purified by Fcoll400 and cultured in RPMI1640.Different concentrations of glucose or mannitol were added into the monolayer of islet cells.The medium insulin concentration was measured by RIA.The apoptosis rate was measured by the flow cytometry and the bax and bcl-2 expressions were detected by immunoblot.Results:①With glucose concentration raised from 11.1 mmol/L to 22.2 mmol/L,the insulin concentration began to raise and reached the peak,then it began to decline.②With glucose at 11.1 mmol/L and 22.2 mmol/L,the apoptosis rate was the highest,while at 5.5 mmol/L,there was no significant difference compared with the controls. For the groups of mannitol-infused,there was no differences of bax or bcl-2 expressions and no difference of the apoptosis rate comparing with the controls.③With glucose infused,the bcl-2 expressions in 11.1 mmol/L and 22.2 mmol/L groups were negative,bax expression was positive;the bax and bcl-2 expressions were negative in the group of 33.3 mmol/L;the bax and bcl-2 expressions in the 5.5 mmol/L group showed no difference compared with the controls.Conclusion:Higher levels of glucose at 11.1mmol/L and 22.2 mmol/L lead to the release of more insulin and the raise of apoptosis rate.But with glucose concentration at 33.3 mmol/L,the release of insulin decreased and the apoptosis rate declined.Hyperosmolality could not cause the apoptosis of the islet cell.

Objective To investigate whether the release of fibroblast growth factor-1 ( FGF-1 ) was changed after inhibition of S100A13 gene (small hairpin RNA, shRNA)and serum-deprivation in human thyroid cancer cells (TT cells ). Methods The S100A13-shRNA pENTRTM/U6 entry vector was transfected into TT cells. The expression of S100A13 mRNA and protein was detected by immunoflurescence, real-time RT-PCR, and Western blot. Then TT cells were treated with S100A13 gene inhibition and serum-deprivation. The changes in release of FGF-1 were detected by indirect immunoflurescence, RT-PCR, and ELISA. Results S100A13 shRNA transfected TT cells (S100A13 RNAi cells)had a reduction of S100A13 gene and protein expression by 80%.Indirect immunofluorescence indicated FGF-1 was mostly localized in the cytoplasm and nucleus of TT cells in primary culture. When serum-deprivation stress was given to TT cells, FGF-1 in cytoplasm almost disappeared in the cells at 6 h. RT-PCR indicated that when serum-deprivation stress was given to TT cells the mRNA of FGF-1 was reduced. ELISA showed that with inhibition of S100A13, the release of FGF-1 was reduced (P＜0.05).Conclusion S100A13-shRNA pENTRTM/U6 entry vector transfected TT cells may inhibit the expression of S100A13 and reduce the release of FGF-1.

With the development of the health service in china,the old talent-training program for clinical medicine major was failed in meeting higher demands for talents of clinical medicine. To create the new talent-training program,therefore,has become the main content of teaching reform for medical col-leges and universities. Taking University of South China as an example,the paper analyzed the changing trend of talent-training program for five-year clinical medicine majors from talent-training goal,curriculum system,practical teaching and content of courses,which proposed some thoughts on the optimization of training program for clinical medicine majors.

Narrative medicine has brought new ideas to medical humanities education, and its con-notation is suitable to Undergraduate Medical Education Standards-Clinical Medicine (Trial). By analyz-ing the proposal and development of narrative medicine, its foundation and core, its programs and methods, as well as its forms and values, the author deepened the understanding of narrative medicine. And through the following four pathways including strengthening the renewal of ideas and concepts on medical humanities quality education, promoting the diversification of teaching ways and methods of medical humanities educa-tion, enhancing the effect of early exposure to clinical medical students, opening the path of integration narrative medicine and evidence-based medicine, the author analyzed narrative medical enlightenment on medical humanities quality education in China.

Objective To observe the effects of aminoguanidine and Vitamin C on lipid of streptozotocin-induced didbetic rats.Methods Diabetic nephropathy rats were induced by intraperitoneal injection of streptozotocin.Fifty Sprague-Dawley(SD) rats were randomly divided into normal control group,model group,Vitamin C group,aminoguanidine group and Vitamin C associated with aminoguanidine group.All the rats were treated for 16 weeks.During and after the treatment,the general state of the rats was observed and the levels of blood sugar,triglyceride,total cholesterol,low density lipoprotein,high density lipoprotein,glycosylated hemoglobin,and glycated low density lipoprotein in different groups were detected.Results The rats in the four groups suffered diabetes mellitus and renal function lesion.Aminoguanidine and Vitamin C could improve the general state of the rats,but had no effect on the blood sugar.Vitamin C could decrease the levels of triglyceride,serum cholesterol,low density lipoprotein,glycosylated hemoglobin,and glycated low density lipoprotein,and increase the level of high density lipoprotein.There was a synergistic effect with the combination of the two drugs. Conclusion Aminoguanidine and Vitamin C have no effect in the decrease of blood sugar level but have a certain effect on regulating blood lipid.There is a synergistic effect with the combination of the two drugs.