OBJECTIVE:To optimize the processing technology for Atractylodes lancea stir-baking with bran. METHODS:Us-ing the content of atractylodin,yield of water-soluble extract and ethanol-soluble extract,appearance score as comprehensive in-dex,the effects of excipient amount,stir-baking time and temperature on the quality of A. lancea stir-baking with bran were investi-gated by Box-behnken design-response surface method,so as to optimize the processing technology parameters of A. lancea stir-bak-ing with bran,and verification test were conducted. RESULTS:The optimized parameters were that the excipients amount was 10% medicinal material,stir-baking at 140 ℃ for 3 min;average comprehensive score of verification test was 0.39 (RSD=2.88%,n=3),which was close to predicted value 0.38. CONCLUSIONS:Box-behnken response surface method is reasonable and feasible for the optimization of the processing technology for A. lancea stir-baking with bran.

Objective To increase curative rate of nonoperation treatment for adult blunt splenic trauma.Methods Data of 141 patients with blunt splenic trauma treated in our hospital from March 2010 to October 2014 were retrospectively analyzed in accordance with the spleen injury degree classification issued by the Spleen Surgery Group of Chinese Society of Surgery in 2000.According to the treatment within 6 hours of injury,these patients were divided into operative and nonoperative management groups.Results The proportion of non operation was 69.5％ (98/141) and the success rate was 89.8％ (88/98).43 cases were in operation group:34 cases in grade Ⅲ,9 cases with grade Ⅳ,3 cases died; 98 cases were in non operation group:including 32 cases of grade Ⅰ,50 cases of grade Ⅱ,15 cases of grade llⅢ,1 case with grade Ⅳ.10 cases failed non operation treatment including 1 case with grade Ⅰ complicated by pancreatic and intestinal injury causing peritonitis during the period of observation,1 case of grade Ⅱ complicated by contusion of the liver and secondary bleeding on day 3 after the trauma,7 cases of grade Ⅲ,were converted to surgery because of multiple organ injury and rebleeding secondary to pulmonary complications,and 1 case of grade Ⅳ with multiple organ injury and shock being immediately converted to surgery.In overall there were 2 deaths in nonsurgery group,1 case with grade Ⅲ and 1 case with grade Ⅳ.Condusions Nonoperative treatment of blunt splenic trauma is safe and feasible in grade up to]Ⅱ blund splenic trauma patients.

Child ; Extraoral Traction Appliances ; Humans ; Molar ; Orthodontic Wires ; Orthodontics, Interceptive ; instrumentation ; Prognosis ; Time Factors ; Tooth Crown ; surgery ; Tooth Eruption, Ectopic ; surgery ; therapy ; Tooth Movement Techniques ; instrumentation ; methods

OBJECTIVETo summarize laws of acupoint selection of prescriptions for treatment of cervicogenic headache by acupuncture in modern literature.

METHODSRandomized controlled trials (RCTs) involving acupuncture and moxibustion for treatment of cervicogenic headache were recruited from CBM (1978-2012), VIP (1989-2012), Wanfang Database (1998-2012), CNKI (1979-2012), PubMed (1966-2012), EMbase (1980-2012), and Cochrane Library (Volume 4, 2012). Hand recruitment was also auxiliarily used. The frequency and percentage of common acupoints, the distribution of acupoints along 14 meridians and across each part of the body, the application of specific acupoints, and features of using prescriptions for specific acupoints were statistically described.

RESULTSTotally 37 recruited papers included 42 acupoints and 159 times. Common acupoints covered Fengchi (GB20, 28 times), Jingjiaji (EX-B2, 21 times), Baihui (DU 20, 12 times), Tianzhu (BL9, 1 times), and Ashi point (11 times). Meridians along which acupoints were used mainly covered Foot-shaoyang Gallbladder Meridian, Foot-taiyang Bladder Meridian,and DU meridian. Acupoints were mainly needled from head, neck, and upper limbs. Eight confluence points and luo-connecting point were commonest used as specific acupoints. Acupuncture prescriptions were mostly composed of multiple acupoints. Filliform needle was mainly used in acupuncture methods, followed by electro-acupuncture needle.

CONCLUSIONSModern acupuncture treatment of cervicogenic headache focuses on local specific points and acupoints along meridians. Acupoints were mostly selected from head, neck, and upper limbs by syndrome typing of Chinese medicine.

Purpose:To explore the role of terminal restric tion fragments (TRFs), telomerase activity and expression of human telomerase re verse transcriptase (hTERT) in colorectal carcinoma. Methods:The telomere length, telomerase activity and expression of hTERT were studied with Southern-blot, TRAP and immunohistochemistry, respe ctively. Results:TRFs in cancer tissue was much shorter than in the adja cent tissues and normal mucosa, and TRFs was decreased gradually along with the development in cancer stage.The expression of Telomerase in colorectal carcinoma tissue was significantly higher than that in other tissues (P

Based on the existing situation of preventive medicine in our country,this article gives insights into the problems and the resolves of college preventive medical education in the field of society,colleges,teaching materials,teachers,students and teaching conditions.

Objective To distinguish primary CMV infection from reactivation or reinfection. Methods A urea denaturation test was included in the washstep of the standard IgG ELISA in 12 patients of age＜12 whose serum CMV-IgG were positive, avidity indexes of CMV-IgG were measured. Results Among children aged 1～12 years, the varieties of avidity indexes of CMV-IgG in A group(IgM negative and IgG positive) and B group(IgM positive and IgG positive) had the same trend. There was only one case in B group whose avidity index was under 30%, the other were all above 30%, and most were above 50%. But among 40 children aged 1～12 months,the avidity indexes were under 30% in 21 cases,8 cases were 30～50% ,and 11 cases were above 50%. The antibody avidity in 1～6 months children was influenced by their mothers lgG,80% (12/15) of 7 ～12 months children had low-avidity antibodies. Conclusion CMV infection mostly occurred among 1～12 months children. Urea denaturation test was an efficient method to distinguish primary CMV infection from reactivation or reinfection.

Objective To establish the normal reference value range of hematological and serum biochemical parameters in Beagle dogs for our own research center and to supply references for establishing the national standard.Methods CV Beagle dogs aged 4～6 months were selected as the investigative objects.Of the Beagle dogs,263 were male with body weight of(8.09?0.78)kg,263 were female with body weight of(6.84?0.87)kg.Blood was collected from cephalic vein at the endgame of the experiment,then both hematological and serum biochemical parameters were measured.The acquired data were analyzed by the "Independent-Samples T Test" process of SPSS V 13.0.Regarded the males and the females as two independent samples,the difference between sexes was also compared.Results The mean normal reference values and the standard deviation of hematological and serum biochemical parameters in both male and female Beagle dogs were obtained by analysis.Conclusions Comparison of sexes showed that there exists obviously difference between male and female Beagle dogs referring to 3 serum biochemical parameters of T-Bil,GLU and ALB.

Objective To observe the effects of gypenosides(GP) on cardiac dysfunction induced by adriamycin in rats and to investigate the therapeutic mechanism.Methods Adult male Sprague-Dawley rats were randomly divided into 5 groups:control group,adriamycin(ADR) group,low-,moderate-and high-dose GP groups.The low-,moderate-and high-dose GP groups received GP 100,200 and 400 mg? kg-1? d-1 for five days respectively.Meanwhile,the purified water was given to rats of control group and adriamycin groups.On the sixth day,adriamycin(10 mg? kg-1) was given intraperitoneally(ip) to all the groups except the control rats.At the twenty-fourth hour after administration of adriamycin,the cardiac functions such as left ventricular systolic pressure(LVSP),maximal rates of increase and decrease of left ventricular pressure(? dp/dtmax),as well as the activities of cardiac ATPase,succinic dehydrogenase(SDH) and sarcoplasmic reticulum Ca2+-ATPase(SERCA2a) were measured.Results The impairment of heart function induced by adriamycin was relieved and the activities of cardiac ATPase,SDH and SERCA2a were obviously increased after GP treatment.Conclusion GP have protective effects on cardiac dysfunction induced by adriamycin in rats,and the mechanism may be related to the increase of activities of cardiac ATPase,SDH and SERCA2a.

Objective:To find out whether the dentin formation of hypophosphatasia children is affec-ted;to study the biological difference of cultured human dental pulp cells from deciduous teeth between hypophosphatasia and normal healthy children.Methods: Anterior deciduous teeth were collected from hypophosphatasia(experiment group)and normal healthy children(control group)respectively.Grounding sections of the affected and healthy deciduous teeth were made to observe their roots;the den-tal pulp cells were separated and cultured.The characteristics of cell proliferation,differentiation and calcification were studied and compared between the two groups of children.MTT assay was performed to study the growth curves of the cells;RT-PCR was performed to evaluate the expression of tissue nonspecific alkaline phosphatase(TNSALP)at different stages;von Kosssa staining was used to test formed calcification nodules after 3 weeks of induction.Results: From the grounding sections,the root surface of the experiment group was not smooth with absorbed areas,cementum was absent.For proliferation activity and the expression of TNSALP,the dental pulp cells from experiment group was obviously lower than control group.Calcified nodules could be seen in both groups,but the nodules were less and the sizes were smaller in experiment group than in control group.Conclusion: In hypophosphatasia patients,not only the cementum but also the dentin were affected.The proliferation,TNSALP expression and calcification capability of the dental pulp cells were influenced in hypophosphatasia patients,and this may be related to the tooth calcification defect.