Objective:To analyze the clinical effect and the preventive analysis of complications on the application of self-prescription soup for eliminating haemorrhoids after patients with moderate or severe hemorrhoids accepted surgery.Methods: 44 patients with moderate and severe hemorrhoids who once accepted operative treatment were divided into observation group and control group as random number table. The patients of control group received the treatment of hip bath by using potassium permanganate tablets, while the patients of observation group received the treatment of hip bath with fuming and washing by using self-prescription soup for eliminating haemorrhoids. The clinically curative effect, postoperative complication, pain, bleeding, edema, disappearance time of anal pendant expansion and healing time of wound between the two groups were compared and analyzed.Results: The total effective rate (100%) of the observation group was significantly better than that (77.27%)of the control group (x2=5.641,P<0.05). The cases of complication including pain, bleeding, constipation, infection, urinary retention and anal stenosis of observation group were significantly less than that of control group (t=7.333,t=15.655,t=5.500, t=4.247,t=8.193,t=5.500,P<0.05). Besides, the times of pain, bleeding, edema, disappearance of anal pendant expansion and wound healing of observation group were significantly, respectively, less than that of control group (t=3.171, t=5.864,t=4.263,t=6.219, t=10.803,P<0.05).Conclusion: The curative effect of self-prescription soup for eliminating haemorrhoids after patient with moderate or severe hemorrhoids accept surgery is significant, and it can effectively prevent and treat various complication.

Adult Stem Cells ; Myocytes, Cardiac ; cytology

Objective: To investigate the correlation between the presence of a crown (CR) or a proximal restoration (PR) and furcation involvement (FI) on molars. Methods: Data were collected from 237 teeth in 44 patients who had restored and non-restored molars with or without FI.The clinical examinations were conducted on the molars including CR,PR,FI, mobility(MO), endodontic treatment(EN) and probe of periodontal attachment level(PAL). Results: Molars with CR or PR had a significantly highter percentage of FI (P0.05) when compared to molars without restorations. Conclusion: The molars with crowns or restorations involving the proximal tooth surfaces have a highter prevalence of FI and more periodontal attachment lose than molars without restorations.

Objective To investigate the surgical effect of hilar cholangiocarcinoma and its impact on prognosis. Method The clinical data of 204 hilar cholangiocarcinoma admitted into the First Hospital of China Medical University were retrospectively analyzed. According to the Bismuth-corlette type, type Ⅰ was 18 cases, type Ⅱ 40 cases, type Ⅲ-a 30 cases, type Ⅲ-b 53 cases, type Ⅳ 57 cases. The other 6 cases was not typed. Color Doppler ultrasound, CT, MRCP were used to determine the Bismuth-Corlette type before the surgery. Based on preoperative image diagnosis the correct diagnosis rate was 53. 7%, 76. 4%, 100% for ultrasound, CT and MRCP respectively. Ninety-two cases underwent tumor resection, including radical resection (R0) in 55 cases, and palliative resection (R1, R2) in 37 cases. Ninety-eight cases underwent exploration and biliary drainage, 6 cases did laparotomy only, 2 cases received liver transplantation. The survival rate (P < 0. 001) is statistically different between patients receiving tumor resection and those not. Radical resection and palliative resection group are also statistically different in survival rates (P < 0. 05). Cox multivariate analysis shows that operation pattern, histological differentiation are two independent prognostic factors. Conclusion Surgery is the main method to treat hilar cholangiocarcinoma and radical resection could achieve the best effect. Reasonable perioperative treatment could reduce the complications and mortality.

Objective To investigate the prognostic factors of hilar cholangiocarcinoma. Methods The clinical data of 204 patients with hilar cholangiocarcinoma who were admitted to The First Affiliated Hospital of China Medical University from January 1996 to May 2007 were retrospectively analyzed. The survival rate was calculated using the Kaplan-Meier method and Log-rank test. Seventeen factors that may have influenced prognosis were analyzed by univariate analysis. Factors that were statistically significant were further analyzed by the Cox regression model. Results The median survival times of patients who received radical resection, palliative resec-tion, bile duct exploration and catheter drainage, exploratory laparotomy, and liver transplantation were 37, 18, 11,5 and 25 months, respectively, and there was a significant difference between the 5 groups (χ2 = 58. 300, P < 0. 05). The prognostic factors included tumor size, portal vein or hepatic artery invasion, local invasion, resection margin, tumor grading, lymph node metastasis and surgical procedure (χ2 =6. 541, 8. 159, 5. 837, 4. 365, 13.748, 5.346, 9.472, P <0.05). Multivariate analysis demonstrated that surgical procedure and tumor grading were independent prognostic factors (6=0.287, 0. 320, P <0.05). Conclusions Radical resection is the most important prognostic factor of hilar cholangiocarcinoma. Appropriate perioperative care can improve the survival rate.

Objective To study parameters influencing the prognosis of patients with gallbladder carcinoma.Methods A retrospective clinical analysis was conducted in 96 cases of gallbladder carcinoma treated in this hospital between 1993 and 2003.Results The overall 5-year survival rate of the patients was 6.32%.The 1-, 3-, and 5-year survival rates following radical resection for gallbladder carcinoma were 78.36%, 48.54%, and 23.87%, respectively.The survival rate was remarkably higher in the radical resection group than in others.Multivariate analysis revealed that depth of infiltration of the tumor and surgical procedure were markedly associated with prognosis.Conclusion Early diagnosis and radical resection are still the mainstay for long-term survival of the patients with gallbladder carcinoma.Appropriate perioperative care can improve survival rate.

BACKGROUND: PEX gene can interfere with the invasion acts of malignant glioma. Bone marrow mesenchymal stem cells (MSCs) are a new type of targeted cell vector on cancer therapy. OBJECTIVE: To construct MSCs stably expressing PEX gene.METHODS: PEX eukaryotic expression vector was constructed by molecular cloning, and identified the recombinant plasmid pcDNA3.1(+)-PEX by restriction endonuclease digestion and sequencing. After transfected with MSCs, the eukaryotic expression vector expression in MSCs was verified by immunocytochemical method. The MSCs stably expressing PEX was established by G418 selection, and then was detected by using reverse transcriptase-polymerase chain reaction.RESULTS AND CONCLUSION: The MSCs stably expressing PEX gene is successfully established, in which PEX gene is highly expressed at both gene level and protein level.

BACKGROUND:Bone marrow mesenchymal stem cells(MSCs)are a kind of adult stem cells with multi-potential differentiation property.At present,it has served as cell carrier for the treatment of Parkinson's disease.OBJECTIVE:To construct pDsRed-C1-CDNF eukaryotic expression vector and induce its expression in rat MSCs.METHODS:CDNF gene was amplified from mouse tissues using RT-PCR,and sequence with Xho I,BamHI restriction enzyme cutting site.The CDNF gene was inserted into the eukaryotic expression vector pDsRed-C1 encoding red fluorescent protein gene.The plasmid pDsRed-C1-CDNF was constructed and transfected into rat bone marrow MSCs.RESULES AND CONCLUSION:The pDsRed-C1-CDNF recombinant plasmid was confirmed by double digestion of Xho I and BamHI restriction enzyme or single digestion of BamHI,and PCR sequence.Results show that the recombinant pDsRed-C1-CDNF eukaryotic expression vector was successfully constructed.

AIM:To evaluate the short-term efficacy of posterior chamber phakic intraocular lens implantation for moderate to high myopia.METHODS:Fifty-three eyes of 27 patients with moderate to high myopia (-3.25 to-21.5D) were received posterior chamber phakic intraocular lens implantation with 2.8mm clear corneal incision.Changes of visual acuity, intraocular pressure(IOP), counts of endothelial cells and surgically induced astigmatism at 1mo after surgery were observed.RESULTS:The preoperative best corrected visual acuity (BCVA LogMAR) were 0.05±0.18, the postoperative BCVA at 1d were 0.02±0.14, the postoperative BCVA at 1wk were-0.05±0.11, the postoperative BCVA at 1mo were-0.07±0.10.The postoperative BCVA were statistically significant differences in different time(P<0.05).The preoperative endothelial cell count was 2932.5±270.8/mm2, the postoperative endothelial cell count at 1d was 2917.1±299.0/mm2;the postoperative endothelial cell count at 1wk was 2902.5±288.6/mm2;the postoperative endothelial cell count at 1mo was 2855.3±284.2/mm2.The postoperative endothelial cell count was not statistically significant differences in different time.The preoperative IOP was 15.8±2.5mmHg;the postoperative IOP at 1d was 15.3±2.9mmHg;the postoperative IOP at 1wk was 17.4±5.8mmHg;the postoperative IOP at 1mo were 15.8±2.6mmHg.The preoperative IOP was significantly different compared with postoperative IOP at 1d, 1wk and 1mo.The postoperative IOP at 1d and 1wk were statistically significant differences (P<0.05).The surgically induced astigmatism at 1d was 0.74±0.39D;the surgically induced astigmatism at 1wk was 0.57±0.28D;the surgically induced astigmatism at 1mo was 0.44±0.21D.The postoperative IOP were statistically significant differences in different time (P<0.05).The preoperative anterior chamber depth was 3.26±0.25mm;the postoperative central vault at 1d were 654.5±345.2μm;the postoperative central vault at 1mo were 683.0±211.7μm.The postoperative central vault were significantly different between that at 1d and 1mo after operations.Operations went without serious complications.CONCLUSION:Posterior chamber phakic intraocular lens implantation for moderate to high myopia were safe, effective and predictability.But long-term effect remains needed to be further follow-up.

Objective To summarize the characteristics of prenatal and postnatal ultrasonic image in hemivertebra fetal malformation. Methods The present study enrolled 12 ultrasound diagnosed hemivertebra fetal malformation and confirmed by either magnetic resonance imaging (MRI) or X-ray in Guangdong Provincial Maternal and Children's Hospital from January 2012 to May 2013. All the ultrasonic image were collected to summarize their characteristics. Results In the twelve cases of prenatal ultrasonic image of hemivertebrae: (1) Vertebral anomaly patterns: the irregular-shape of bones are smaller than normal vertebral bony hyperechoic which inserted into the normal intervertebral in wedge-shape, scoliosis or angular deformity. (2) Diseased parts:in the 12 fetuses, 5 had single vertebral deformity while the other 7 had multiple vertebral deformities. For the location of the lesion, there were 6 dysmorphia appeared in thoracic, 3 appearedin lumbar, 1 appeared in sacrococcygeal, 1 appeared in both cervical and thoracic, and 1 appeared in both thoracic and lumbar. (3) Associated with other malformations: 4 term delivery fetuses confirmed diagnosis of hemivertebra malformation by MRI (the diagnosis of hemivertebra was confirmed by both the fetal MRI and postnatal X-ray in 2 of them within 3 days);8 fetuses had additional anomalies, including heart malformations. Three of them were induced abortion, the X-ray images of the fetal sample showed hemivertebradysmorphia. Five women chosed induced laborin local hospital, and the X-ray images of the fetal were diagnosed as hemivertebradysmorphia. One of them with rib deletion in thoracic was missed diagnosed. Conclusions Three-dimensional ultrasonicimages can clearly show the degree of scoliosis, locate vertebra. It provides important imaging evidence for the treatment of fetal dysmorphiaafter labor.