Objective:To observe the level of endogenous cortisol in patients with advanced gastrointestinal cancer, and to evaluate the effect of exogenous glucocorticoids treatment on patient's malignant symptoms and serum cortisol level. Methods:Thirty-one patients with advanced gastrointestinal cancer who were given 40 mg/d methylprednisolone for continuous 5 days. The patients included 8 patients with esophageal and cardial cancer, 12 patients with gastric cancer, and 11 patients with colorectal cancer. At the same time, 30 healthy adults were selected as control group. The cortisol radioactive immunoassay kit was used to determine the level of serum cortisol in the gastrointestinal cancer patients and controls. The life quality changes of gastrointestinal cancer patients were evaluated. Results:The serum cortisol level of 31 advanced gastrointestinal cancer patients was higher than that of healthy volunteers [(312.39±57.64)vs(144.64±52.20) μg/L,P<0.01]. The average serum cortisol levels in the patients with esophageal cancer, gastric cancer and colorectal cancer were higher than healthy controls (P<0.05). The serum cortisol level in gastric cancer patients was the highest among the three types of gastrointestinal cancers (P<0.01). After being treated with exogenous glucocorticoids, the five parameters such as appetite, spirit, sleeping, fatigue and pain in gastrointestinal cancer patients were improved significantly. During the course of exogenous glucocorticoid treatment, there were no serious complications. The serum cortisol levels after exogenous glucocorticoid treatment did not change significantly in the patients with esophageal cancer, gastric cancer and colorectal cancer (P>0.05). Conclusion:Endogenous serum cortisol levels of gastrointestinal cancer patients were significantly higher than those of healthy adults. Short-term treatment with medium dosage of exogenous glucocorticoids had little effect on the endogenous serum cortisol level.

BACKGROUND:Studies have shown that simvastatin can promote bone formation, but there is stil controversial on the osteogenic mechanism and osteogenic effect.
OBJECTIVE:To explore the osteogenesis effect of the composite of simvastatin and Bio-Oss versus simple Bio-Oss material on the repair of rabbit mandibular defects.
METHODS:Twelve New Zealand white rabbits were selected to establish alveolar bilateral mandibular defects models. The composite of simvastatin and Bio-Oss was implanted randomly in one side of defect region;Bio-Oss was simply implanted in the other side of defect region. Both sides were covered with Bio-Gide bilayer col agen membrane. Four rabbits in each group were sacrificed at 4, 8 and 12 weeks after implantation, and the general observation X-ray film, oral cone-beam CT imaging observation and histopathologic study and quantitatively were conducted to quantitatively and qualitatively comparative analyze the alveolar bone formation in the graft region.
RESULTS AND CONCLUSION:At 4, 8 and 12 weeks after implantation, new bone formation was found and increased with time prolonging. With the gradual degeneration of high resistance fire Bio-Oss bone meal, the bone mineral density at different time points of the simvastatin composite Bio-Oss group was lower than that of the simple Bio-Oss group (P<0.05). The percentage of bone formation in the simvastatin composite Bio-Oss group was significantly higher than that in the simple Bio-Oss group (P<0.05). Simvastatin could accelerate Bio-Oss degradation and promote new bone formation in bone defects repairing.

Characters of one Candida intermedia yeast strain which isolated from nature can produce ethanol from xylose-fermenting been systemic studied. In conditions 28?C, 120 r/min, 72 h, it can produce 6.480 g/L ethanol from 7% xylose and 43.70% theoretical production of ethanol from 3% xylose. It can produce up to 21.225 g/L ethanol when incubation time prolong to 156 h from 8% xylose. It also can ferment 13% glucose produce 47.647 g/L ethanol and reach 76.90% of theoretical ethanol production, respectively. Compared to CK, ethanol productivity can be improved 9.91% when add 8% xylose in three times as 3%, 2% and 3%, respectively. Glucose can be first utilized in the mixture sugar medium. When the ratio of xylose vs. glucose is 3:1in mixture sugar, the productivity of ethanol can be improving 25%.

Professor LI Ying-cun believes that pediatric internal injury cough is due to the weakness of lung and spleen and endogenous phlegm. The lung loses dispersing and descending to retrograde and cause cough; spleen deficiency produces phlegm to exist in the lung. The children have delicate organs need strengthening the body resistance, dispersing lung and invigorating spleen, supplementing qi and consolidating the exterior.ZisuDecoction is one of the TCM prescriptions that found from Dunhuang ancient medical prescriptions. Professor LI Ying-cun flexiblely uses this ancient prescription in modern clinic, which has achieved good efficacy in treatment of pediatric internal injury cough.

Vascular smooth muscle cell (VSMC) proliferation and migation play important roles in the occurrence and development of atherosclerosis and restenosis after intravascular stenting.The studies in recent years have shown that kallikrein-kinin system (KKS) is closely correlated with VSMC proliferation and migration in cytokines and transduction pathways.Therefore,investigating the roles of KKS in the VSMC proliferation and migation process has great significance in clinical prevention and treatment of atherosclerosis and restenosis after intravascular stenting.

Response surface analysis (uniform precision of central composite design, SAS 9.1.3 software) was applied to optimize the four major factors (ratio of soybean meal to water, enzyme quantity, fermentation time and inoculation quantity) for soybean meal solid fermentation. According to the change of the hydrolyzation degree of soybean protein, the equation of polynomial regression was established between those factors and the response. The result showed that the optimum condition included as follows: ratio of soybean meal to water 1∶1.00,enzyme quantity 2.55%, fermentation time 65h and inoculation quantity 1.00%. Under the optimum level, the degree of hydrolyzation reached 13.3%, which increased 56% over pre-optimization.

BACKGROUND:Studies have found that the platelet-rich plasma (PRP) can promote bone and soft tissue injury repair, but its effect on the process of bone healing is stil controversial.
OBJECTIVE:To contrastively observe the osteogenesis effect of PRP/Bio-Oss/Bio-Gide in the repair process of alveolar bone defect in rabbits, so as to explore the role of PRP in bone healing.
METHODSixteen New Zealand white rabbits were used to establish animal models of critical-size alveolar bone defect. One side was damaged randomly and repaired by PRP/Bio-Oss/Bio-Gide as experimental side, and the other side repaired by Bio-Oss/Bio-Gide as control side. Four animals were executed at each time-point, postoperative weeks 2, 4, 8, 12. Through general observation, X-ray radiograph, Cone Beam CT assessment, histological examination, the osteogenesis effect in the defect region was qualitatively and quantitatively analyzed.
RESULTS AND CONCLUSION:It could be know from each observation index that as time went on, the experimental and control sides had a different degree of new bone formation and the degradation-absorption of bone graft material. At 12 weeks, continuous cortical bone formation was seen on the surface of the experimental side, new bone formed and tended to be mature, obvious degradation of the bone graft was found, but those in the control side were not as good. At each time-point of 2, 4, 8, 12 weeks, the bone mineral density of the experimental side were lower than that of the control side (P<0.05), but the percentage of new bone area was larger than in the experimental side than the control side (P<0.05). These findings indicate that the PRP/Bio-Oss/Bio-Gide has a better osteogenesis effect than the Bio-Oss/Bio-Gide in the repair process of alveolar bone defect in rabbits, and PRP can promote new bone formation and degradation-absorption of Bio-Oss.

Objective To approach the methods and effects of internal fixation for Hoffa fracture. Methods A total of 26 patients with 26 condylar Hoffa fractures ( medial condylar fractures in 13patients and lateral condylar fractures in 13) treated from August 1993 to February 2010 were retrospectively analyzed.According to the Letenneur classification,there were 16 patients with type Ⅰ fractures,four with type Ⅱ fractures and six with type Ⅲ fractures.Among them,two patients were with open fractures and 24 with closed fractures.Surgical approaches including screw fixation in 21 patients and lateral support plate fixation in five were selected based on the fracture types and affected sides. Results All patients were followed up for 12.5-48 months (average 18 months),which showed fracture healing in all the patients within 3-4 months (average 3.5 months).Two patients had slight shift together with knee joint pain,ie,one patient had ROM of knee for 95 °,and one failed the functional exercise because of pain and had ROM of knee for 60° during follow up.No complications including infection,delayed union or bone necrosis occurred.According to Letenneur' s functional assessment system,the postoperative outcomes were excellent and good in 24 condyles,fair in one and poor in one. Conclusions Surgical treatment for Hoffa fractures is safe and effective,but the key point is to choose correct screw fixation position and orientation according to the fracture types and fracture fragment size.

Objective To evaluate CT findings, diagnosis and management of intrahepatic biliary injuries after transcatheter arterial chemoembolization (TACE) for liver malignancies. Methods A total of 1302 patients with hepatic malignant tumors received TACE between Sep 2007 and Mar 2009. None of these patients were found to have any radiographic evidence of biliary abnormalities before TACE. A retrospective review of imaging studies and clinical outcomes was carried out to evaluate the changes of intrahepatic biliary injuries after TACE. Results Six patients developed intrahepatic biliary injuries during 1 and 3-month follow-up. Two cases with jaundice and high fever, underwent percutaneous transhepatic cholangiography and drainage and eventually recovered. The other 4 patients were asymptomatic with only radiographic changes and were managed observationally. Conclusions Intrahepatic biliary injury is a rare complicating TACE procedures. It will be identified by clinical outcomes and radiographic imaging findings. Percutaneous transhepatic cholangiography and drainage (PTCD) is the therapy of choice for intrahepatic biliary injuries with jaundice or high fever.

Objective To investigate the treatment measures of having trigeminocardiac reflex (TCR) for the primary trigeminal neuralgia via microvascular decompression.Methods From January 2016 to December 2016,the clinical data and anesthesia records of 79 consecutive patients with primary trigeminal neuralgia admitted to the Department of Neurosurgery,Xuanwu Hospital,Capital Medical University and treated the first craniotomy microvascular decompression were analyzed retrospectively.They were divided into a no TCR group (n=63) and a TCR group (n=16) according to the intraoperative anesthesia monitoring.The differences of baseline conditions,preoperative complications and pain involved the trigeminal nerve branches were compared between the two groups.The treatment measures and short-term prognosis of the patients with TCR during operation were summarized.Results (1) The proportion of hypertension in the TCR group was 31.2％ (n=5),it was higher than 7.9％ in the no TCR group (n=5).There was significant difference (χ2=6.273,P<0.05).(2) During the operation of microvascular decompression,16 patients in the TCR group had 19-time TCR.The baseline heart rate was 74±10/min before operation,and the heart rate decreased to 51±6/min at onset of TCR.The mean baseline arterial blood pressure was 102±13 mmHg,and the mean arterial blood pressure decreased to 74±8 mmHg at the onset of TCR.There was significant difference (P<0.05).(3) After the onset of TCR,the operation of was stopped,heart rate and the blood pressure recovered spontaneously (n=4).Fifteen patients were treated with medications,including using atropine (12 times,dose 0.2-0.5 mg) and ephedrine hydrochloride (3 times,dose 5-10 mg).Heart rate and blood pressure returned to the baseline level within 20 seconds or 20 seconds after administration of medications.There was no TCR-related cardiac complication or neurological deficit from after procedure to before discharge.Conclusions In microvascular decompression of primary trigeminal neuralgia,if TCR occurs,the selective use of anticholinergic agents or vasoactive agents is needed as early as possible.The risk factors for the occurrence of TCR need to be further verified.