1.Expression and detection of recombinant directing toxin SL120 in Pichia pastoris.
Hong WANG ; Ning-Yi JIN ; Ge-Fen YIN ; Yi-Ming XU ; Hong-Tao JIN ; Li-Shu ZHANG ; Zi-Jian LI
Chinese Journal of Biotechnology 2005;21(3):473-477
Anti-HIV-1 gp120 single chain antibody(scFv) gene and staphylococcus extoxin A(SEA) gene were inserted into vector pPIC9K. The recombinant plasmid was integrated into Pichia pastoris by electroporation. High level expression was performed by determining the Muts phenotype and screening muti-copy integrants. The recombinant protein was about 57kD and the production was 50.1 mg/L. It was shown that the two kinds of protein affected the conformation of each other by antibody affinity assay, but the recombinant targeting toxins could highly mediate CTLs to kill HIV-1 target cells.
Enterotoxins
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genetics
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Genetic Vectors
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HIV Envelope Protein gp120
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biosynthesis
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genetics
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immunology
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HIV-1
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genetics
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immunology
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Immunoglobulin Variable Region
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biosynthesis
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genetics
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immunology
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Pichia
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genetics
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metabolism
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Recombinant Proteins
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biosynthesis
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genetics
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immunology
2.Sequencing and phylogenetic analysis for non-structure protein coding region of dengue virus from Yunnan border.
Shu-you YUAN ; Xue-min CAI ; Fu-qiang ZHANG ; Wei QIU ; Gang-shan LI ; Hua LIU ; Ge-fen YIN ; Zuo-sheng LI ; Ying ZHENG ; Shuang-yin WANG ; Hai-lin ZHANG ; Quan-shui FAN
Chinese Journal of Epidemiology 2010;31(7):830-831
3.Inverted U-Shaped Associations between Glycemic Indices and Serum Uric Acid Levels in the General Chinese Population: Findings from the China Cardiometabolic Disease and Cancer Cohort (4C) Study.
Yuan Yue ZHU ; Rui Zhi ZHENG ; Gui Xia WANG ; Li CHEN ; Li Xin SHI ; Qing SU ; Min XU ; Yu XU ; Yu Hong CHEN ; Xue Feng YU ; Li YAN ; Tian Ge WANG ; Zhi Yun ZHAO ; Gui Jun QIN ; Qin WAN ; Gang CHEN ; Zheng Nan GAO ; Fei Xia SHEN ; Zuo Jie LUO ; Ying Fen QIN ; Ya Nan HUO ; Qiang LI ; Zhen YE ; Yin Fei ZHANG ; Chao LIU ; You Min WANG ; Sheng Li WU ; Tao YANG ; Hua Cong DENG ; Jia Jun ZHAO ; Lu Lu CHEN ; Yi Ming MU ; Xu Lei TANG ; Ru Ying HU ; Wei Qing WANG ; Guang NING ; Mian LI ; Jie Li LU ; Yu Fang BI
Biomedical and Environmental Sciences 2021;34(1):9-18
Objective:
The relationship between serum uric acid (SUA) levels and glycemic indices, including plasma glucose (FPG), 2-hour postload glucose (2h-PG), and glycated hemoglobin (HbA1c), remains inconclusive. We aimed to explore the associations between glycemic indices and SUA levels in the general Chinese population.
Methods:
The current study was a cross-sectional analysis using the first follow-up survey data from The China Cardiometabolic Disease and Cancer Cohort Study. A total of 105,922 community-dwelling adults aged ≥ 40 years underwent the oral glucose tolerance test and uric acid assessment. The nonlinear relationships between glycemic indices and SUA levels were explored using generalized additive models.
Results:
A total of 30,941 men and 62,361 women were eligible for the current analysis. Generalized additive models verified the inverted U-shaped association between glycemic indices and SUA levels, but with different inflection points in men and women. The thresholds for FPG, 2h-PG, and HbA1c for men and women were 6.5/8.0 mmol/L, 11.0/14.0 mmol/L, and 6.1/6.5, respectively (SUA levels increased with increasing glycemic indices before the inflection points and then eventually decreased with further increases in the glycemic indices).
Conclusion
An inverted U-shaped association was observed between major glycemic indices and uric acid levels in both sexes, while the inflection points were reached earlier in men than in women.
Aged
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Asian Continental Ancestry Group
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Blood Glucose/analysis*
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China/epidemiology*
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Cohort Studies
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Diabetes Mellitus/blood*
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Female
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Glucose Tolerance Test
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Glycated Hemoglobin A/analysis*
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Glycemic Index
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Humans
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Male
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Middle Aged
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Uric Acid/blood*