Based on the literature review of medical behaviors,big data mining and related rules,the paper introduces the basic contents of big data mining and related rule algorithm-Apriori algorithm,takes partial Electronic Medical Records (EMR) system data of the First Affiliated Hospital of Zhengzhou University as the basis,conducts mining analysis on factors such as the gender,age,operation records,critical ill records,visiting distance and hospital days of a patient by adopting Apriori algorithm of related rule,obtains the rules of medical behaviors of patients of different visiting distances,and puts forward relevant policies and suggestions.

[Summary] The study summarized surgical cooperation key points during percutaneous transforaminal endoscopic lumbar discectomy combined with radiofrequency in 60 cases, including preoperative preparation, intraoperative care, and maintenance and sterilization of instruments after the surgery.All the 60 operations were successfully completed, without complications such as intraoperative dural rupture and nerve root injury.Postoperatively, an instant relief of low back pain was obtained and the lower limb straight leg raising test showed negative immediately.The preoperative preparation and proficiency with special surgical skills are conducive to successful operation and improvement of surgical outcomes.

Aim To investigate the effects of osthol on bone marrow stromal stem cells in vitro under the con-ditions of the ability to differentiate into osteoblasts and the case of proliferation. Methods The rat bone marrow sample was obtained,and the all bone marrow cell culture methods were used to separate and collect the stratum of mononuclear cells. The cells were cultured in DMEM containing 10% fetal bovine serum. Three days later the culture medium was changed for the first time. Nine days later,serial subcultivation proceeded. The final concentration of osthol was 1 ? 10 -4,1 ? 10 -5,1 ? 10 -6,1 ? 10 -7 mol?L -1 respectively. MTT method was adopted in proliferation analysis. Under the induced condition,the Alkaline phosphatase activity, calcium salt sediment yield and osteocalcin were meas-ured on 4th,8th,12th,16th day. On the fifteenth day,his-tochemistry dyeing proceeded for calcified tubercle. Total RNA was isolated and the gene expression of bFGF, IGF-1,Osterix and Runx-2 was investigated by RT-Real Time PCR. Results The BMSCs proliferation was refrained by osthol dose-dependently. But it evidently led to osteogenesis. The ALP activity,calcium salt sediment yield and osteocalcin were raised,and calcified tubercle amount was increased. Besides,it also could enhance the mRNA level of bFGF,IGF-1,Osterix and Runx-2. Conclusion The osthol with final concentra-tion of 1 ? 10 -5 mol?L -1 can markedly promote BM-SCs differentiation to osteogenesis. which proves osthol is an active constituent of the traditional Chinese medi-cine Common Cnidium Fruit.

120 age- and body mass-matched Lewis rats underwent anastomosis of vessels and bronchus using cuff technique and 60 rats received orthotopic left lung transplantation. All procedures were done by one operator. The feasibility and reliability of this technique was determined by examining graft patency usng X-ray, gross and histological examinations. The successful rate of the surgery was 96.7%, mean warm ischemic time was (14.5±3.2)min. On gross examination, grafts were well perfused and ventilated.Recipients of 14d after transplantation showed excellent chest X-ray signs and perfect graft function. The animal model established in this study has been verified to be a safe, simple and reproducible model.

To revise Chinese Diet Balance Index (DBI) based on the current Chinese Dietary Guidelines and Food Guide Pagoda . Method: Seven food components [(cereals ,vegetables and fruits, bean and dairy , animal food , alcohol ,condiments (salt and edible oil))were selected in combination with food varieties and drinking water to build up Diet Balance Index Revision (DBI-07) .The components were weighed and assigned cut-offs and scores based on the Chinese Food Guide Pagoda. Three scoring methods (LBS-low bound score, HBS-high bound score, DQD-diet quality distance) were used to evaluate the dietary quality of 26300 adults aged 18 to 79 years from 2002 China National Nutrition and Health Survey. Results: The mean LBS, HBS and DQD were 11.5,30.0 and 41.5 respectively. About 78% adult were in moderate or severe deficit of food intake, 35.1% in moderate or severe surplus of food intake, and 43.8% in severe dietary imbalance. Conclusion: DBI-07 can reflect the principles of dietary guidelines, and the scoring method can reflect both deficit and surplus of food intake. DBI-07 can be used to evaluate the dietary quality of individual or the population.

This study is to explore that how to use the information management system to research composed of the management and sharing of research projects,study exchanging and technical guidance.The purpose is to improve the management efficiency and level of scientific research projects.Current study aimed to explore establish a digital management system

lymphocytic thyroiditis.

This study is to investigate the effects on the expression of iNOS and production of NO in the osteogenic differentiation process of rat bone marrow stromal cells (rBMSCs) by icariside II. rBMSCs were cultured by adherence screening method. When the culture dishes were covered with 80% cells, the osteogenic induced cultures were adopted. Icariside II was supplemented into the culture at 1 x 10(-5) mol x L(-1). The activity of iNOS, content of NO and osteogenic differentiation markers including alkaline phosphatase (ALP) activity, CFU-FALP and mineralized bone nodules were compared among the icariside II-supplemented group, L-NMAE group, icariside II + L-NAME group and the control. Total RNA was isolated and the gene expression of iNOS, Osterix and Runx-2 was investigated by real-time PCR. Total protein was also isolated and the secretion of iNOS and collagen I was examined by Western blotting. Icariside II can significantly improved ALP activity, CFU-FALP amount and mineralized nodules. Besides, the mRNA level of factors related to the osteogenic differentiation includes Osterix and Runx-2 also enhanced. The secretion of collagen I also promoted significantly. But all of these effects can be inhibited by L-NAME which can specifically inhibit the activity of iNOS. Icariside II enhances the osteogenic differentiation of rBMSCs significantly, but if the activity of iNOS was blocked by L-NAME, the osteogenic differentiation markers decrease accompanied with iNOS and NO decrease, suggesting that icariside II stimulates the osteogenic differentiation via enhancing the activity of iNOS and promoting the generation of NO.

Objective To analyze the distribution, diversity, and the sensitivity of common pathogen species to antifungal drugs in fungal keratitis during 2000 - 2006 in Shandong province. Methods Samples from corneal serapings and corneal buttons from keratoplasties were used for fungal culture and identification. The distribution and diversity of the pathogens in different years were analyzed. The sensitivity of some common species to antifungal drugs were tested. Results A total of 898 patients were diagnosed as fungal keratitis, in which 770 (85.7%) were positive in fungal culture. Pathogens in 748 cases caused by fungal infection were identified, of which 547 cases were caused by Fusarium (71.0%). The prevelence of Fusarium from 2000 to 2006 was 75.5%, 72.8%, 71.5%, 75.2%, 76%, 68. 8% and 56.4% respectively. Eighty-four cases were caused by Aspergillus( 10.9% ) , which accounted for 15.1% , 15.2% , 13.1%, 10. 2%, 10. 4% , 8.0% and 6. 9% of the cases from 2000 to 2006, respectively. Seventy-four eases were caused by Alternaria (9. 6% ), the percentage of which during 2000 -2006 was 1. 9%, 3.3%, 3.7%, 6. 6%, 8. 8%, 12. 0 and 29. 7% respectively. In genus Fusarium, 10 species were found and the most common species were Fusarium Solani ( 33. 8% ), Fusarium oxysporum ( 28.2% ) and Fusarium moniliforme( 27.4% ). Six species were identified in genus Aspergillus, with Aspergillus flavus (53.6%) and Aspergillus fumigatus (39.3% ) being the most common species. The geometric mean MICs of amphotericin B, terbinafin, itraconazole, 5-flucytosine and fluconazole against the common species were 0. 647, 0. 714, 1. 624, 15. 108 and 27. 070 μg/ml, respectively. The mean MICs of the above five antifungal drugs against Fusarium were 0. 899, 0. 893, 3.077, 37. 124 and 21. 687 μg/ml, respectively, and for Aspergillus, the mean MICs of these agents were 0.794, 0.591, 0.416, 23.973 and 7.127 μg/ml, respectively; for Alternaria,the MICs were 0. 409,0. 479,0. 433,11. 655 and 7. 104 μg/ml ,respectively. Conclusions Genus Fusarium, Aspergillus and Alternaria were the predominant pathogens in fungal keratitis in Shandong Province. During 2000 -2006, Fusarium was the most common one, followed by Aspergillus. The percentage of Aspergillus decreased annually. The third most common pathogen, Alternaria increased during the period. The geometric mean MICs of amphotericin B and terbinafin against Fusarium were lower than those of itraconazole, 5-flucytosine and fluconazole. The MICs of these five anti-fungal drugs against Aspergillus and Alternatia were lower than those for Fusarium.

Objective To investigate the accuracy of CTVision image-guided stereotactic body radiation therapy (SBRT) for non-small cell lung cancer (NSCLC).Methods 10 lung SBRT patients were imaged with CTVision before and after irradiation for acquiring and analyzing the three-dimensional set-up error data sets in our department from October 2010 to May 2012.Gross tumor volumes (GTVs) were contoured on pre-and post-SBRT CT sets and combined for generating internal gross tumor volumes (IGTVs).Planning target volume (PTV) margin was calculated,and IGTVs and PTVs were compared for off-line verification of accuracy of SBRT.A paired t-test statistical analysis was conducted with the datasets using SPSS 17.0.Results 80 CT image sets were totally obtained.Setup errors was significant difference before and after radiotherapy in the left-right,superior-inferior and anterior-posterior directions,that were (-0.10±1.30) mmand (-0.15±1.31) mm (P=0.720),(0.18±1.32) mm and (0.18±1.43) mm (P =1.000) and (-0.08 ± 1.19) mm and (-0.13 ± 1.18) mm (P =0.750),respectively.IGTVs of ten patients were smaller than corresponding PTVs (13.53 cm3 and 37.84 cm3,P =0.000).Conclusion Accuracy and safety of SBRT could be verified by imaging with CTVision before and after delivery for non-small cell lung cancer.