Objective To investigate the causes of the complications occurred after repeated embolization therapy for huge cerebral arteriovenous malformations and to discuss their nursing interventions. Methods A total of 54 embolization procedures were performed in 17 patients with huge cerebral arteriovenous malformations. The clinical data were retrospectively analyzed. The causes of complications were carefully examined and the preventive measures were discussed. The prompt and necessary nursing interventions were formulated in order to prevent the complications or serious consequences. Results Among the total 17 patients, one patient gave up the treatment because of the cerebral hemorrhage which occurred two months after receiving 3 times of embolization therapy. One patient experienced cerebral vascular spasm during the procedure, which was relieved after antispasmodic medication and no neurological deficit was left behind. Two patients developed transient dizziness and headache, which were alleviated spontaneously. One patient presented with nervousness, fear and irritability, which made him hard to cooperate with the operation and the basis intravenous anesthesia was employed. No complications occurred in the remaining cases. Conclusion The predictive nursing interventions for the prevention of complications are very important for obtaining a successful repeated embolization therapy for huge cerebral arteriovenous malformations, which will ensure that the patients can get the best treatment and the complications can be avoided.

Acupuncture Therapy ; Adult ; Aged ; Ankle ; Female ; Humans ; Male ; Middle Aged ; Stroke ; physiopathology ; therapy ; Wrist

Acupuncture Therapy ; Aged ; Aged, 80 and over ; Female ; Humans ; Male ; Middle Aged ; Pain ; etiology ; Pain Management ; Stroke ; complications

Glucuronosyltransferase ; genetics ; Humans ; Infant, Newborn ; Jaundice, Neonatal ; genetics

Traditional knowledge digital Library (TKDL) is a mature database for protection of traditional knowledge from illicit aggression, and also used for searching and examining the novelty and inventive step of applied inventions. TKDL has the role of defensive protection on Indian traditional medicine through promoting legislation initiatively, signing TKDL access agreement with international intellectual property authorities, and establishing close cooperation with WIPO. TKDL is a typical and successful case. Based on the publication and promotion ways of traditional knowledge digital library and the actual national condition in China, this paper proposes several applicable ways for publication and promotion of traditional knowledge protection directory of traditional Chinese medicine database.

Objective To explore the application of bacterial community diversity profiling in forensic comparison of soil.Methods The DNA profilings of bacterial community from 5 soil samples of different source and 4 samples of same source were obtained through the specific amplification of 16S rDNA segments followed by terminal restriction fragment length polymorphism(T-RFLP)analysis,and the similarity indexes between different soil samples were calculated.Result The ranges of similarity indexes between different sources of soil samples were 0.3～0.44,and the ranges between same sources of soil samples were 0.76～0.87.Conclusion There is a great relativity between the bacterial community diversity profiling and the source of soil sample.

OBJECTIVE:To establish a preparative separation method for euphol reference substance in Euphorbia pekinensis. METHODS:Parts of petroleum ether extraction from E. pekinensis ethanol extract were separated by silica gel column chromatogra-phy with petroleum ether-ethyl acetate(95∶5-70∶30,V/V)by gradient elution. The enriched fractions of euphol were collected. With the methanol repeated recrystallization,nuclear magnetic resonance spectroscopy(NMR)method,mass spectrometry(MS)meth-od and other spectroscopic methods were used to identify the chemical structures,and thin layer chromatography (TLC),UV, HPLC-UV,HPLC-MS were combined to detect the mass fraction. RESULTS:The mass fraction of euphol reference substance sepa-rated from E. pekinensis was>99%. CONCLUSIONS:The reference substance of euphol acquired by this method meet the relative requirements of the chemical reference substance in the content dertermination of TCM new drug quality standard. It provides chemi-cal reference substance for the quality control of E. pekinensis and prescription preparations containing E. pekinensis and the basic research of effective substances.

Objective The objective of this study was to investigate the expression of PSMD10 and Ki67 in epithelial ovarian tumors and its clinicopathological significance.Methods Immunohistochemical method was used to detect the expression of PSMD10 and Ki67 in 21 cases of ovarian benign tumor,31 cases of ovarian borderline tumor and 156 cases of ovarian cancer.Results The positive expression rate of PSMD10 and Ki67 in ovarian cancer was significantly higher than those of ovarian borderline and benign tumor(P<0.05).The positive expression rate of PSMD10 in ovarian cancer tissues was not different from others groups(P>0.05).There was significant different the positive expression rate of PSMD10 from clinical stage,pathological grade and residual tumor diameter(P<0.05).There was a positive correlation between the expression of PSMD10 and Ki67 in ovarian cancer(P<0.01).Conclusion PSMD10 and Ki67 play an important synergistic role in the carcinogenesis and progression of epithelial ovarian cancer.It can be used as an important index to judge the degree of malignancy and progression of ovarian cancer.Joint testing PSMD10 and Ki67 are used to guide the clinical diagnosis.

OBJECTIVE:To establish HPLC method for the simultaneous determination of calcium lactate and calcium gluconate in compound calcium gluconate oral solution.METHODS:The test was performed on C 18 column with column tem?perature at25℃;the mobile phase was composed of0.025mol/L NaH 2 PO 4 (pH=2.50)with a flow rate of1.0ml/min and sam ple size of20?l;the detection wavelength was210nm.RESULTS:The calibration curves were linear over the range of0.719～46.040mmol/L(r=0.9999)for lactic acid and0.315～20.160mmol/L(r=0.9999)for gluconic acid.The average recovery rates of which were99.0%(RSD=1.65%)and100.5%(RSD=1.36%),respectively.CONCLUSION:This method is fast,simple,accurate,and it can be employed directly without isolation to determine the content of calcium lactate and calcium glu?conate simultaneously and to control the quality of compound calcium gluconate oral solution as well.

Objective To investigate the changes in counts of T-lymphocyte subsets, natural killer cell (NK cell) and the levels of serum soluble interleukin-2 receptor(sIL-2R) after perioperative allogeneic whole-blood transfusion or erythrocyte suspention (SAG-M blood) blood transfusion. Methods Thirty patients undergoing elective refection for rectal cancer, were randomly allocated to receiving 400 ml of allogeneic whole-blood (n = 15 ) or 400 ml of SAG-M blood (n = 15 ) during perioperative period, respectively. Indirect immunofluorescence technique and enzyme-linked immunosorbent assay were used to determine the counts of T--lymphocyte subsets, NK cells and to detect levels of serum sIL-2R 1 day and 5 day after operation.Results The counts of CD3 +, CD. + and NK cells in both groups decreased significantly after operation (P