With the population aging, diet changing and incveasing risk factors on vascular disease, the lower extremity ischemic disease has become a frequently occurring disease of older person, and it is the main reason for amputation disability. In the 21st century the stem cells transplantation is one of the most advanced technologies and has been applied quickly to clinical therapy, regarded as a radical treatment of lower extremity ischemic disease. Endothelial progenitor cells have gradually become a new direction and a new research focus because of its unique biological characteristics in the treatment of this disease. This article focuses on endothelial progenitor cells in treating extremity ischemia lesions on the theoretical basis and research developments.

Objective To observe the clinical effect of Angelica injection combine with psychological intervention in treatment of patients with lower limb fracture postoperative analgesia.Methods 60 cases underwent lower limb fracture surgery from February 2013 to April 2015, and were randomly divided into experimental group and control group, 30 cases in each group.Control group were treated with conventional medicine epidural injection morphine for postoperative analgesia, experimental groups were injected with Angelica injection treatment in control group on the basis of conventional therapy, recorded and analyzed two groups of patients improved postoperative pain scores, postoperative stress and pain after treatment. Results After treatment, 4, 8, 24, 48h pain scores of experimental group were lower than of control group (P<0.05); After 4h, 48h post-operation heart rate and mean arterial blood pressure was better than of control group (P<0.05).Conclusion Effect of conventional western medicine combined with Angelica injection postoperative analgesia is superior to conventional medicine alone , with no obvious side effects.

Objective After partial hepatectomy, hepatopoietin(HPO) increase rapidly. The present study was designed to investigate how rhHPO and partial hepatectomy induce the expression of immediate early gene. Methods The Wister rats were divided into hepatectomy group and control group. The different gene expression within 1 h after 2/3 partial hepatectomy and in primary cultured hepatocytes system were examined by using representational difference analysis. Results In the expressed sequence tag(EST) library, most of these genes were identified as immediate early genes, and one new gene Tec that might be associated with liver regeneration in the EST library was found. Moreover, the Tec gene was rapidly induced by 2/3 partial hepatectomy. The expression peak achieved within 1 2 h after operation. HPO can rapidly induce the expression of these genes(c fos, LRF 1 and Tec, etc.) in primarily cultured rat hepatocytes. Conclusion rhHPO and partial hepatectomy can rapidly induce the expression of immediate early gene. This is the first report that Tec gene is an immediate early gene related to liver regeneration.

Objective To investigate whether host collateral vessels play a role in the cardiac repair induced by intracoronary injection of bone marrow mononuclear cell (BMMNCs) in a swine myocardial infarction (MI) model. Methods MI was induced in swine models by ligation of left anterior descending artery. Two weeks later, twenty animals with Rentrop score=0 (R0) or Rentrop score=1 (R1) received either intracoronary PBS or BM-MNCs (2?10~8 cells) infusion. Results At 6 weeks after MI, Rentrop score was significantly increased in the R1+BMT group. Ejection fraction and fractional shortening evaluated by echocardiography were increased in both R0+BMT and R1+BMT groups and the greatest improvements were seen in the R1+BMT group. Consistent with the changes in cardiac function and Rentrop score, the greatest degree of angiogenesis and cell count of engrafted BM-MNCs occurred in the R1+BMT group 6 weeks post MI. Conclusion The existence of intrinsic collateral vessels contributes to the beneficial effects for cardiac repair post MI by intracoronary BM-MNCs transplantation.

0.05). Conclusion Though the noninvasive MRCP offers a diagnostic means equivalent to that of ERCP it is still too early to say it will take the place of ERCP.

Objective To compare the detection rates of capsule endoseopies with push endoseo-pies. Methods From May 2002 through January 2003 , thirty - nine patients with suspected small bowel diseases, particularly the gastrointestinal bleeding of unknown origin were examined by capsule endoscopies. Of the 39 patients. 32 complained of obscure recurrent gastrointestinal bleeding. From January 1993 to October 1996, 36 patients suffered from unexplained GI bleeding underwent push endoscopies. All patients had prior normal results on gastroseopy, colonoscopy, small bowel barium radiography, seintigraphy and/ or angiogra-phy. Results M2A capsule endoscopies disclosed abnormal small bowel findings in 26 out of 32 patients (81% ). Twenty one of 26 patients had significant pathological findings in explaining their clinical disorders with diagnostic yield of 66% (21 of 32 patients). Definite bleeding sites diagnosed by capsule endoscopies in 21 patients including angiodysplasia 8, inflammatory small bowel diseases 5, small bowel polyps 4, GI stro-mal tumor 2,earcinoid tumor and lipoma 1 , and bemorrhagie gastritis 1. Push endoscopies detected the definite sources of bleeding in 9 of 36 patients (25%). Definite bleeding sources included angiodysplasia 2, leiomyosareoma 2, leiomyoma 1 , lymphoma 1 , Grohns disease 1 , small bowel polyps 1 ,and adenocareinoma of ampulla 1. Suspected bleeding sources were seen with push endoscopies in two additional patients, and other five patients with capsule endoscopies. Conclusion Gapsule endoseopy is superior to push endoscopy in detecting obscure GI bleeding ( P