Objective To establish a method for determination of trace lead in drinking water by intermittent flow HG-AFS was developed. Methods The conditions for K3Fe(CN)6-KBH4-HCl system of generation of PbH4 were optimized and interference of coexisting ions were tested. Results The fluorescence signal of blank solution was decreased evidently by treating K3Fe(CN)6 with activated carbon and the detection limit was effectively improved. The interference of coexisting ions could be suppressed in some degrees in the presence of K3[Fe(CN)6]. Under the optimized conditions, the detection limit of this method was 0.10 ?g/L and RSD(n=6) was in the rang 0.4%-3.8%. The recovery rate was 95.0%-112.0%. Conclusion This method was accurate, simple, sensitive and suitable for determination of trace lead in drinking water.

Objective To observe the effect of marin algae polysaccharide derivant on the osteoporotic model of the rats. Methods Forty six-month-old female Wistar rats were selected to duplicate the osteoporotic model induced by retinoic acid and performed with marine algae polysaccharide derivant. The rats were randomly divided into two groups: experiment group treated with 10 mg marine algae polysaccharide derivant10mg per day by oral,control group treated with 10 mg glucose per day by oral.The effect of marine algae poly saccharide derivant on osteoporosis were evaluated by morphometry. Results After intake of polysaccharide,the mean trabecular plate density (Tb.N),the mean trabecular plate thickness(Tb.Th),and the percent trabecular area (Tb.Ar%) of t he experiment group, which were treated by polysaccharide were significantly increased more than that of the control group model(P<0.01). Conclusion These results indicate that polysaccharide can increase bone mass and has a therapeutic and preventional effect on the osteoporotic model of the rats induced by retinoic acid.

The same paraffin-embedded specimen of skin biopsy from each of 29 patients with cutaneous malignant lymphomas (CML) and 7 patients with cutaneous lymphoid infiltrates (CLI) was estimated simultaneously and retrospectively by karyomorphometry and proliferating rate assay. The results showed that the differences in nuclear volumes (NVs), count of argyrophilic nucleolar organizer regions(AgNORs) and proliferating cell nuclear antigen (PCNA-PC10) indices (PC10Is) between CML and CLI were statistically very significant. The NVs and PC10Is were correlated to the death rate of patients. NVs were also correlated to AgNORs.

Objective:To translate the English version of Edmonton Frail Scale into Chinese version and to test its reliability and validity.Methods:The translation and cross-cultural adaptation of the English version of Edmonton Frail Scale were generated by Beaton's translation and back-translation method. From April to August of 2019, at the department of geratology of the First affiliated Hospital of China Medical University in Shenyang, a sample of 303 hospitalized elderly adults who met the inclusion and exclusion criteria were selected via convenience sampling method to test its psychometric properties.Results:The scale level content validity index and item level content validity index of the Chinese version of the Edmonton Frail Scale were both 1.0. The criterion validity between the Chinese version of Edmonton Frail Scale and the Tilburg Frailty Indicator was 0.723 ( P<0.01), confirmed by Pearson correlation coefficients. Using the Tilburg Frailty Indicator as an external criterion, the Edmonton Frail Scale showed satisfactory diagnostic accuracy for frailty (area under the curve=0.924). The optimal cut-point for frailty was 6 (sensitivity: 77.6%, specificity: 94.7%). More frail individuals were recognized by the Chinese version of Edmonton Frail Scale among older and female participants than their counterparts( P<0.05). The Cronbach'α of the Chinese version of Edmonton Frail Scale was 0.599, the test-retest reliability within a 7-day interval of the scale was 0.822. Conclusions:The Chinese version of Edmonton Frail Scale presents good validity and reliability and can apply to Chinese hospitalized elderly people.

Objective: To explore a scientific, complicated sampling method and corresponding formulas for sensitive question survey. Methods: Cochran W. G.'s classic sampling theories, two randomized response technique(RRT) models, total probability formulas, and properties of mean and variance were used in this study. Results: Formulas for the estimation of the population proportion and its variance for dichotomous sensitive questions on Greenberg model in cluster sampling and stratified cluster sampling were deduced. Formulas for the estimation of the population mean and its variance for quantitative sensitive question on the additive constant model in cluster sampling and stratified cluster sampling were deduced. Our survey methods and formulas were successfully applied in surveying the pre-marriage sex and cheating in examination in Soochow University. Conclusion: Our survey methods and formulas on two RRT models for sensitive question survey in cluster sampling and stratified cluster sampling have high reliablility.

Adolescent ; Adult ; Female ; Histiocytic Necrotizing Lymphadenitis ; diagnosis ; therapy ; Humans ; Lymph Nodes ; Male ; Middle Aged ; Neck ; Young Adult

The peak of the brain edema after intracerebral hemorrhage usually begins after 3 to 4 days.A large quantity of hemoglobin releases and degrades during this process,and its degradation products iron ion,bilirubin and carbon monoxide,etc play important roles in the process of cerebral edema.They all involve in the formation Of delayed brain edema after intracerebral hemorrhage.The research of hemoglobin and its degradation products is expected to find out a new way for the treatment of intracerebral hemorrhage.

Objective To investigate the expression of miR-155 ,nuclear factor kappa B (NF-κB) and soluble intercellular adhesion molecule -1 (sICAM-1 ) in peripheral blood in patients with type 2 diabetes mellitus (T2DM ) ,and to explore the role of miR-155 in vascular lesions of T2DM. Methods A total of 165 T2DM patients and 60 health subjects from health examinations were enrolled in this study. All the subjects were divided into two groups :subjects without vascular lesions group and vascular disease group. Vascular disease group was further divided into microangiopathy group ,macroangiopathy group , microangiopathy+ macroangiopathy group ;based on 24 hUAlb level normal urinary albumin (NAU ) group ,microalbuminuria (MAU) group ,macroalbuminuria (MAAU) group. MiR-155 ,NF-κB and sICAM-1 in peripheral blood were tested by RT-PCR. Single factor analysis of variance was used for comparison among groups. Stepwise regression analysis was used for correlation factors analysis. Results The expression of miR-155 ,NF-κB and sICAM-1 were significantly higher in T2DM group than in NC group [(1.82 ± 0.71) vs (1.00 ± 0.12) ,(2.28 ± 0.66) vs (1.04 ± 0.33) ,(1.88 ± 0.80) vs (1.03 ± 0.30) ,P<0.05]. The level of miR-155 ,NF-κB ,sICAM-1 were significantly higher in T2DM vascular lesions group than in subjects without vascular lesions group [(1.95 ± 0.73) vs (1.34 ± 0.29) ,(2.40 ± 0.65) vs (1.65 ± 0.16 ) ,(2.01 ± 0.77 ) vs (1.07 ± 0.41 ) ,P < 0.05 ]. The expression of miR-155 was higher in microangiopathy+ macroangiopathy group than in macroangiopathy group [(2.36 ± 0.61 ) vs (1.77 ± 0.59) ,P < 0.05 ].The expression of NF-κB was also significantly higher in microangiopathy +macroangiopathy group than in microvascular disease group and macroangiopathy group (P<0.05). The level of miR-155 was significantly higher in group MAU group and MAAU group than in NAU group [(1.41 ± 0.49) ,(2.64 ± 0.52) vs (1.04 ± 0.20) ,P<0.05] ,and NF-κB and sICAM-1 were also higher than NAU group (P<0.05). Multiple stepwise regression analysis showed that miR-155 was positively correlated with NF-κB and sICAM-1(t=4.235 ,9.728 ,P<0.01). Conclusion The expression of miR-155 increases in T2DM patients with vascular complications ,and this trend is the same as NF-κB and sICAM-1. It suggests that miR-155 maybe involves in the pathogenesis of diabetic chronic vascular disease.

Objective: To provide a scientific method for the reasonable use of the drugs,and to use it as guidelines for clinical chemotherapy. Methods: Samples of test group were obtained from 38 cases of bladder cancer.which were cultivated with different medicines and then examined the inhibiting rate with MTT.The medicine with the highest inhibiting rate was selected to treat bladder cancer.30 cases of bladder cancer were treated with HCPT routinely as control group.The rate of tumor recurrence between two groups was compared. Results:The inhibiting rate on tumour cells was positively related to the concentration of drugs,the combined chemotherapy was superior to single drug.Statistic results showed significant difference between the test group and control group(P

AIM: Eye surface burn is easy to cause blindness. Amniotic membrane transplantation has become a hot spot of research for its special effects in recent years. This study observed the therapeutic outcome of fresh amniotic membrane transplantation for acute chemical or thermal burns at eye surface. METHODS: ①Eight eyes with acid burn in eight cases, ten eyes with alkali burn in ten cases, and seven eyes with thermal burn in six cases were selected from Yan'an University Affiliated Hospital between January 2002 and June 2007 including 23 males and 1 female aged 16-50 years. There were 18 eyes with preoperative vision