1.Review on Gastric Mucosal Microbiota Profiling Differences in Patients with Chronic Gastritis, Intestinal Metaplasia, and Gastric Cancer.
The Korean Journal of Gastroenterology 2014;64(6):390-393
No abstract available.
Bacteria/*isolation & purification
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Female
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Gastric Mucosa/*microbiology
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Gastritis/*microbiology
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Humans
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Intestinal Diseases/*microbiology
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Male
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*Microbiota
2.Helicobacter pylori: Bacterial Strategy for Incipient Stage and Persistent Colonization in Human Gastric Niches.
Kwang Ho RHEE ; Jin Sik PARK ; Myung Je CHO
Yonsei Medical Journal 2014;55(6):1453-1466
Helicobacter pylori (H. pylori) undergoes decades long colonization of the gastric mucosa of half the population in the world to produce acute and chronic gastritis at the beginning of infection, progressing to more severe disorders, including peptic ulcer disease and gastric cancer. Prolonged carriage of H. pylori is the most crucial factor for the pathogenesis of gastric maladies. Bacterial persistence in the gastric mucosa depends on bacterial factors as well as host factors. Herein, the host and bacterial components responsible for the incipient stages of H. pylori infection are reviewed and discussed. Bacterial adhesion and adaptation is presented to explain the persistence of H. pylori colonization in the gastric mucosa, in which bacterial evasion of host defense systems and genomic diversity are included.
Gastric Mucosa/*microbiology
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Gastritis/*microbiology/pathology
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Helicobacter Infections/*microbiology
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Helicobacter pylori/*physiology
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Humans
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Stomach Neoplasms/pathology
3.Relationship between oral and gastric Hp infection and diseases of upper digestive tract in children.
Yun-guang BAO ; Yi WEI ; Ai-su LANG ; Wei-jun YU ; Ai-juan YING ; Lin-qing MU ; Hui-xian YANG
Chinese Journal of Pediatrics 2003;41(6):465-466
Adolescent
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Child
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Child, Preschool
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Female
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Gastric Mucosa
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microbiology
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Gastrointestinal Diseases
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diagnosis
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microbiology
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Helicobacter Infections
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diagnosis
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microbiology
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Helicobacter pylori
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growth & development
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isolation & purification
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Humans
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Infant
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Male
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Mouth Mucosa
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microbiology
4.Helicobacter pylori in Dental Plaque and Saliva.
Nayoung KIM ; Seon Hee LIM ; Kye Heui LEE ; Jun Young YOU ; Jung Mogg KIM ; Na Rae LEE ; Hyun Chae JUNG ; In Sung SONG ; Chung Yong KIM
The Korean Journal of Internal Medicine 2000;15(3):187-194
BACKGROUND: About half of the world population is infected with H. pylori, but the transmission and the source of this infection are still unclear. Recently, dental plaque (DP) and saliva have been implicated as possible sources of H. pylori infection. This study was done to investigate the detection rates of H. pylori in the DP and saliva by use of PCR depending on H. pylori infection state of gastric mucosa. METHODS: In 46 subjects, gastric H. pylori colonization was evaluated with CLO test, microscopy of Gram stained mucosal smear, culture and histology after modified Giemsa staining in the antrum and body, respectively. A patient was regarded as H. pylori positive if one or more of the four aforementioned test methods demonstrated H. pylori colonization of the gastric mucosa. For detection of H. pylori in the DP and saliva, PCR assay was done with ET4-U and ET4-L primers. To estimate the sensitivity and specificity of this PCR, H. pylori positivity was evaluated in the antrum and body, separately. RESULTS: The sensitivity of mucosal PCR was 50.0% (27/54) and the specificity 86.8% (33/38). When a subject was regarded as H. pyloi positive, if either antrum or body mucosal H. pylori was is positive, the positive rate of mucosal PCR was 62.1% (18 subjects) in the 29 H. pylori-positive and 17.6% (3 subjects) in the 17 H. pylori-negative subjects. DP PCR was positive in 2 of 29 H. pylori-positive subjects (6.9%) and none in the 17 H. pylori-negative (0%). Saliva PCR was positive in 4 of 14 H. pylori-positive subjects (28.6%) and none of 6 H. pylori-negative (0%). CONCLUSION: The detection rates of H. pylori in DP and saliva by PCR were rather low, 6.9% and 28.6%, respectively, and these rates might have been underestimated by low sensitivity of the PCR method used in this study. However, the results that H. pylori was found in the DP and saliva suggest that the oral cavity can perform a role as a reservoir of H. pylori in Korea.
Dental Plaque/microbiology*
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Gastric Mucosa/microbiology
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Helicobacter pylori/isolation & purification*
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Human
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Polymerase Chain Reaction
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Saliva/microbiology*
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Sensitivity and Specificity
5.Adherence of Helicobacter pylori to areas of type II intestinal metaplasia in Korean gastric mucosa.
Tae Jung JANG ; Jung Ran KIM ; Dong Hoon KIM
Yonsei Medical Journal 1999;40(4):392-395
The aim of this study was to examine whether Helicobacter pylori (H. pylori) attaches to areas of intestinal metaplasia in Korean patients. Gastric biopsy specimens with intestinal metaplasia from 8 gastric cancers, 24 gastric ulcers, 11 duodenal ulcers, and 57 chronic gastritis were examined. The specimens were stained with periodic acid-Schiff/alcian blue pH 2.5 and high-iron diamine/alcian blue pH 2.5 to identify the subtype of intestinal metaplasia, and then immunohistochemical stain was done with rabbit anti-H. pylori polyclonal antibody. In 17 patients, H. pylori attached to areas of type II intestinal metaplasia. All areas of intestinal metaplasia showing adherence contained sialomucin, and H. pylori was not detected in the areas of intestinal absorptive cells and sulfomucin-containing metaplastic cells.
Adult
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Aged
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Bacterial Adhesion/physiology*
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Female
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Gastric Mucosa/pathology*
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Gastric Mucosa/microbiology*
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Helicobacter pylori/physiology*
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Human
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Intestines/pathology*
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Intestines/microbiology*
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Korea
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Male
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Metaplasia
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Middle Age
6.Establishment of Helicobacter pylori infection model in Mongolian gerbil.
Jie YAN ; Ai-Ping HU ; Qiang LI
Journal of Zhejiang University. Medical sciences 2003;32(1):21-23
OBJECTIVETo establish a stable and reliable model of Helicobacter pylori infection in Mongolian gerbil and to observe pathological changes in gastric mucosa from the infected animals.
METHODSMongolian gerbils were randomly divided into six groups infected with H.pylori strain NCTC11637 (n=6, group N), six groups infected with H.pylori clinical strain Y06 (n=6, group Y) and six groups as negative control (n=4, group C). H.pylori suspensions at the concentrations of 2 X 10(8)CFU/ml and 2 X 10(9) CFU/ml of strain NCTC11637 and strain Y06 were prepared with Brucella broth from Columbia agar containing sheet blood. The animals in one group N and in one group Y were orally challenged once with 0.5 ml of 2 X 10(8) CFU/ml H.pylori suspension. The animals in another group N and in another group Y were orally challenged with 0.5 ml of 2 X 10(9) CFU/ml H.pylori suspension for three times at the intervals of 24 hours, respectively. The animals were killed after 2nd, 4th and 6th week of the last infection and the gastric mucosal samples were taken for urease test, bacterial isolation, routine pathological and H.pylori histochemical examinations.
RESULTSInfection rates of the animals in group N and group Y at the 2nd, 4th and 6th week after one challenge were 0%, 0%, 66.7% and 0%, 16.7%, 16.7%, respectively. Infection rates of the animals in groups N and Y at the 2nd, 4th and 6th week after three challenges were 66.7%, 100%, 100% and 66.7%, 66.7%, 100%, respectively. In animals with positive bacterial isolation H.pylori was found to colonized on the surface of gastric mucosal cells and in the gastric pits, and the lamina propria of gastric mucosal was infiltrated with chronic inflammatory cells.
CONCLUSIONBy using H.pylori suspension at high concentration of 1 X 10(9) CFU for multiple times, the orally challenged Mongolian gerbils can be prepared as a stable and reliable H.pylori infection model. H.pylori can colonize in gastric mucosa of the infected animals, and mild inflammation reactions can be seen.
Animals ; Disease Models, Animal ; Female ; Gastric Mucosa ; microbiology ; Gerbillinae ; Helicobacter Infections ; microbiology ; pathology ; Helicobacter pylori ; isolation & purification ; Immunohistochemistry
7.The Role of Gastric Acid in the H. pylori-induced Gastritis in Mouse.
Sung Soo KIM ; Yeo Min LEE ; Hyung Keun KIM ; Jin Il KIM ; Hiun Suk CHAE ; In Sik CHUNG ; Doo Ho PARK
The Korean Journal of Gastroenterology 2007;50(6):363-369
BACKGROUND/AIMS: This study was designed to investigate the role of gastric acid in the extent of H. pylori-induced gastritis. METHODS: Twenty eight mice were innoculated with live H. pylori. They were allocated into four groups. Mice in group I received no treatment, group II mice were treated with sham injection, group III received 125microgram/kg body weight of pentagastrin, while group IV received 250microgram/kg body weight of pentagastrin subcutaneously three times a week. After 7 months, the mucosal pH, H. pylori density, neutrophils and monocytes infiltration, and the degree of atrophy were assessed in the stomach. RESULTS: In the gastric body, the densities of H. pylori were not different among groups. The degree of neutrophil infiltration was significantly lower in group IV compared to other groups (p<0.05). The degree of monocyte infiltration was also significantly lower in group IV than group III (p<0.05). In the gastric antrum, there was no significant difference of the H. pylori density, neutrophil and monocyte infiltration, and degree of atrophy among the groups. The mice with the gastric mucosal pH lower than mean of 3.2 had significant lower level of H. pylori density (1.4 vs. 2.4, p=0.04), and infiltration of neutrophils (0.9 vs. 2.3, p=0.018), and monocytes (1.2 vs. 1.8; p=0.011) than the those with mucosal pH above 3.2 in the body of stomach. CONCLUSIONS: Gastric acid plays a role in suppressing the proximal propagation of H. pylori-induced gastritis to the body of stomach.
Animals
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Female
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Gastric Acid/*metabolism
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Gastric Mucosa/pathology
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Gastritis/immunology/*microbiology
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Helicobacter Infections/*immunology/microbiology
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*Helicobacter pylori/isolation & purification
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Hydrogen-Ion Concentration
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Mice
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Mice, Inbred C57BL
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Models, Animal
8.Effect of Acupuncture in the Treatment of Young Pigs with Induced Escherichia coli Diarrhea.
Eun Sung PARK ; Seona JO ; Je Kyung SEONG ; Tchi Chou NAM ; Il Suk YANG ; Min Cheol CHOI ; Yeo Sung YOON
Journal of Veterinary Science 2003;4(2):125-128
The effect of acupuncture in the treatment of young pigs with induced enteropathogenic Escherichia coli diarrhea was histopathologically evaluated by routine hematoxylin and eosin stain. Thirty two pigs weighed 4-5kg and aged 21days old were used in this study. The animals with diarrhea were treated with traditional acupuncture, or enrofloxacin. In the group treated with traditional acupuncture, acupoint GV1 (Jiaochao) was used and in the group treated with antibiotics, enrofloxacin was injected intramuscularly. Ten pigs were inoculated with E. coli, but were not treated and served as nontreated control group. At postinoculation day 6, all pigs of the acupuncture and antibiotic treated groups recovered from diarrhea. In the ascending and descending colons of the nontreated control group, severe infiltration of inflammatory cells in the lamina propria was observed and in the fundic stomach, destruction of the fundic gland architecture and necrotic lesions were observed, however, in the same sites of the acupuncture and antibiotics treated groups, the mucosae of the colon and stomach were relatively similar to those of the normal group. These results indicate that acupuncture treatment is effective in controlling induced E. coli diarrhea in pigs at its early stage.
Acupuncture
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Animals
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Colon/cytology/microbiology/pathology
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Diarrhea/therapy/*veterinary
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Escherichia coli Infections/therapy/*veterinary
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Gastric Mucosa/cytology/microbiology/pathology
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Intestinal Mucosa/cytology/microbiology/pathology
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Male
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Stomach/cytology/microbiology/pathology
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Swine
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Swine Diseases/*microbiology/therapy
9.Detection rate of Helicobacter pylori and its clinical significance in children with Meckel's diverticulum.
Shi-Qiong ZHOU ; Hong MEI ; Xu-Fei DUAN ; Guo-Gang YE ; Bao-Xiang WANG
Chinese Journal of Contemporary Pediatrics 2014;16(3):238-241
OBJECTIVETo determine the detection rate of Helicobacter pylori (Hp) in children with Meckel's diverticulum (MD) and its clinical significance among children with MD.
METHODSEighty-one children with MD were divided into two groups according to the presence (n=45) or absence (n=36) of digestive hemorrhage. The detection rates of Hp in MD tissues and stomach tissues were determined by immunohistochemistry. The detection rates of Hp were compared between the two groups and between the MD tissues with different clinical features in the hemorrhage group.
RESULTSThe detection rate of Hp in MD tissues for the hemorrhage group was 76% (34/45), which was significantly higher than that for the non-hemorrhage group (47%, 17/36) (P<0.05). The detection rate of Hp in stomach tissues for the hemorrhage group (87%, 39/45) was insignificantly higher than that for the non-hemorrhage group (67%, 24/36) (P>0.05). Among patients in the bleeding group, the detection rate of Hp in MD tissues showed no relationship with age, sex, preoperative hemorrhage frequency, amount of hemorrhage, length of MD, basal diameter of MD, and pathological type (P>0.05), but was related to location of MD, presence or absence of ulcer, and depth of ulcer (P<0.05). For the hemorrhage group, a significant positive correlation was found between the detection rates of Hp in MD tissues and stomach tissues (P<0.05), as shown by the Spearman correlation analysis.
CONCLUSIONSThe detection rate of Hp in MD tissues is increased in children with MD complicated by digestive hemorrhage. Hp infection may play some role in the hemorrhage process among children with MD.
Adolescent ; Child ; Child, Preschool ; Female ; Gastric Mucosa ; microbiology ; Gastrointestinal Hemorrhage ; etiology ; microbiology ; Helicobacter pylori ; isolation & purification ; Humans ; Immunohistochemistry ; Infant ; Male ; Meckel Diverticulum ; complications ; microbiology