No abstract available.
Fruit* ; Gardenia* ; Sputum* ; Yeasts*

Gardenia ; Iridoids ; Pigmentation ; Plant Extracts

OBJECTIVETo compare the contents of iridoid among the fruit, seed, and pericarp from Gardenia jasminoides.

METHODThe contents of geniposide and genipin gentiobioside were determined simultaneously by HPLC. A Kromasil C18 column at 35 degrees C was used with the acetonitrile-0.3% formic acid anhydrous (12: 88) as the mobile phases. The detection wavelength was set at 238 nm and the flow rate was 1.0 mL x min(-1).

RESULTThe obtained linearity of the two components was better over 0.999 4 and the average recoveries were 99.11%, 98.47%, respectively. The results exhibited the remarkable variation among the different parts of the fruit.

CONCLUSIONThe contents of the two constituents in seed were higher than in pericarp.

Fruit ; chemistry ; Gardenia ; chemistry ; Iridoids ; analysis ; Seeds ; chemistry

The chromatic values of the broken-fried and single-fried Gardeniae Fructus Praeparatus(GFP) were measured by the color analyzer to analyze the color variation rule, and the contents of 10 main components were determined by ultra-high performance liquid chromatography(UPLC). The multivariate statistical analysis, Pearson correlation analysis, and discriminant analysis were conducted to investigate the color and components of GFP samples. The experimental results revealed that L~*, a~*, b~*, and E~*ab decreased continuously during processing, and the color of samples gradually deepened. The trend and range of chromatic values during broken-frying and single-frying processes were basically identical. Gardenoside, crocin-Ⅰ(C-Ⅰ), and crocin-Ⅱ(C-Ⅱ) showed an obviously downward trend, while the contents of geniposidic acid and 5-hydroxymethylfurfural(5-HMF) increased significantly. Shanzhiside, deacetyl-asperulosidic acid methyl ester, and geniposide(G2) showed a downward trend. Scandoside methyl ester rose first and fell later. Genipin-1-O-gentiobioside(G1) went through a decrease-increase-decrease trend. The change trends of component contents during broken-frying and single-frying processes were generally consistent, but the change range was different. Among all the components, scandoside methyl ester and G1 showed obvious change. Because of different stir-frying time, the change rate of each component content in the process of broken-frying was higher than that in single-frying process. Additionally, geniposidic acid, gardenoside, scandoside methyl ester, C-Ⅰ, C-Ⅱ, and 5-HMF exhibited a higher correlation with apparent color. On the basis of above findings, the discriminant function of two frying processes was established, which could be applied to the discrimination of broken-fried and single-fried samples. This study analyzed the dynamic quality change rule of GFP during broken-frying and single-frying processes based on color-component correlation analysis, and found the two methods showed consistent change trend, yet with slight difference in the quality of samples. This study can provide data support for the processing of GFP.
Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; Fruit ; Gardenia

The chromaticity space parameters of the samples during the processing of Gardeniae Fructus Praeparatus(Jiaozhizi in Chinese herbal name, JZZ) were measured by the visual analyzer to analyze the color variation rule during the processing of JZZ, and the content changes of total reducing sugar, total amino acid and 5-hydroxymethylfurfural(5-HMF) related to Maillard reaction were measured. Pearson correlation analysis and linear regression analysis of the data were carried out by SPSS 24.0 software. The experimental results showed that the objective coloration of the samples in the processing of JZZ was basically consistent with the traditional subjective color judgment; the contents of total reducing sugar and total amino acids showed a decreasing trend during the processing of JZZ, and the content of 5-HMF showed an increasing trend, which was in line with Maillard reaction law. Pearson correlation analysis results showed that there was a significant correlation between the chromaticity space parameters L~*(lightness value), a~*(red green value), b~*(yellow blue value), E~*ab(total color value) and the contents of total reducing sugar, total amino acid and 5-HMF(P<0.01), among which the values of L~*, a~*, b~*, E~*ab were positively correlated with the contents of total reducing sugar and total amino acid, and negatively correlated with the contents of 5-HMF. The results of linear regression analysis also showed that these two were highly correlated. In this study, by establishing the correspondence relationship between the color change of JZZ processing and Maillard reactants, wecan not only provide a basis for the objective digital expression of subjective color of JZZ, but also provide a reference for explaining the processing mechanism of JZZ from a new perspective.
Amino Acids ; Color ; Drugs, Chinese Herbal ; Fruit ; Gardenia ; Maillard Reaction

In order to realize rapid and non-destructive identification of the origin of Gardeniae Fructus, a technical method based on hyperspectral imaging technology was established in this study. Spectral information of Gardeniae Fructus samples from eight production origins was acquired from visible NIR(410-990 nm, VNIR) and short wavelength NIR(950-2 500 nm, SWIR) bands based on hyperspectral imaging techniques. The average spectral reflectance within the region of interest was extracted and calculated using the ENVI 5.3 software, resulting in 1 600 sample data. The visible short wavelength infrared band(fused bands) spectral data covering the range 410-2 500 nm were obtained after combining the spectral data of VNIR and SWIR. Data were de-noised by five common preprocessing methods, including multivariate scatter correction, Savitzky-Golay smoothing, standard normal variate, first derivative(FD), and second derivative from VNIR, SWIR, and fused bands(VNIR+SWIR). Partial least squares discriminant analysis, linear support vector classification(LinearSVC), and random forest were used to establish the model for origin identification of Gardeniae Fructus. The results indicated that the identification model of Gardeniae Fructus origin established after FD pretreatment of the spectral data in the fused bands could yield good results. According to the confusion matrix evaluation results, the model prediction set using LinearSVC reached 100% accuracy, so the optimum identification model of Gardeniae Fructus origin was determined as fusion bands-FD-LinearSVC. Therefore, the hyperspectral imaging technology can achieve rapid, nondestructive, and accurate identification of Gardeniae Fructus samples of different origins, which provides a technical reference for the differential detection of Gardeniae Fructus and other Chinese medicines.
Gardenia ; Hyperspectral Imaging ; Fruit ; Least-Squares Analysis ; Technology

Genipin, an aglycone derived from geniposide found in Gardenia jasminoides, is known to be an excellent natural cross-linker, strong apoptosis inducer, and antiviral agent. Although evidence suggests antiviral activity of genipin in several in vitro viral infection systems, there have been few literatures which review antitumor effects of genipin in a variety of in vitro/in vivo models of cancers yet. In this review, we present some of the latest findings in the studies of genipin focusing on antitumor effects and its mechanisms. In brief, genipin inhibits mitochondrial uncoupling protein 2 to increase accumulation of reactive oxygen species, leading to ROS/c-Jun N-terminal kinase-dependent apoptosis of cancer cells. Genipin also increase tissue inhibitors of metalloproteases (MMP), resulting to decrease activities of MMP-2 which plays a key role in metastasis of cancers. Genipin has shown a biphasic effects on cell death and survival in cancer cells as many other plant-derived phytochemicals do. Finally we discuss the potential of genipin as a promosing novel antitumor agent which could be applicable to chemotherapy and/or chemoprevention for cancers.
Apoptosis ; Cell Death ; Chemoprevention ; Drug Therapy ; Gardenia ; In Vitro Techniques ; Metalloproteases ; Neoplasm Metastasis ; Phytochemicals ; Reactive Oxygen Species

Iridoid glycosides (mainly geniposide) and crocetin derivatives (crocins) are the two major active constituents in Gardenia jasminoides Ellis. In the present study, geniposide, crocins, crocin-1 and crocetin were separated from gardenia chromatographically. Then, mice were orally administrated with geniposide (400 mg/kg b.w.), crocins (400 mg/kg b.w.), crocin-1 (400 mg/kg b.w.) and crocetin (140 mg/kg b.w.) once daily for 7 days with CCl4. Hepatoprotective properties were evaluated by biochemical parameters: Administration of geniposide, crocins, crocin-1and crocetin significantly lowered serum alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) levels in CCl4-treated mice. The reduced glutathione (GSH) levels and antioxidant enzymes (SOD and CAT) activities were also increased by geniposide, crocins, crocin-1 and crocetin. Histopathological examination of livers showed that these components reduced deformability, irregular arrangement and rupture of hepatocyte in CCl4-treated mice. These biochemical results and liver histopathological assessment demonstrated that geniposide, crocetin derivatives and crocetin show comparative beneficial effects on CCl4-induced liver damage via induction of antioxidant defense. Therefore, contents of geniposide and crocetin derivatives should be both considered for hepatoprotective efficacy of Gardenia jasminoides Ellis.
Alanine Transaminase ; Alkaline Phosphatase ; Animals ; Aspartate Aminotransferases ; Gardenia ; Glutathione ; Hepatocytes ; Iridoid Glycosides ; Liver* ; Mice* ; Rupture

To introduces the body process of iridoid in gardenia and effect of biological activity of enzymes systematically and discusses the mechanism of these compounds on the basis of the domestic and foreign recent literatures. It also provides a literature basis for the instruction of rational clinical prescription, reform of dosage forms, and development and utilization.
Animals ; Enzyme Activation ; drug effects ; Enzymes ; metabolism ; Gardenia ; chemistry ; Humans ; Iridoids ; metabolism ; pharmacology

OBJECTIVETo compare the contents of diterpenoid pigments among the fruit, seed, and pericarp from Gardenia jasminoides.

METHODThe separation of Crocin 1, Crocin 2, Crocin 3 were carried out simultaneously on a Kromasil C18 column at 35 degrees C with the methanol-acetonitrile-0.3% formic acid anhydrous in gradient elution as the mobile phrases. The detection wavelength was set at 440 nm and the flow rate was 1.0 mL x min(-1).

RESULTThe obtained linearity of the three components was better over 0.999 5 and the average recoveries were 97.50%, 98.76%, 97.67%, respectively. The results exhibited the remarkable variation among the different parts of the fruit.

CONCLUSIONThe contents of three constituents in seed were higher than in pericarp.

Carotenoids ; analysis ; Diterpenes ; analysis ; Fruit ; chemistry ; Gardenia ; chemistry ; Pigments, Biological ; analysis