Objective To observe the effects of phloroglucinol and diazepam on the progression of labor.Methods Two hundred normal primipara without indication of cesarean were randomly divided into intervention group and control group.When the cervix dilated 3 cm,the intervention group was given 80 mg phioroglueinol by injection of the cervix and 10 mg diazepam by injection of muscle,the control group was not given any drug.The length of labor stage,the different ways of labor,scores of neonate,volume of bleeding during 2 hours after labor of the two groups were observed.Results The pain degree of Ⅰ,Ⅱ,Ⅲ grade was 82,16,2 cases in intervention group,but 30,58,12 cases in control group,there was significant difference between the two groups(P＜0.01).The length of total labor stage and the first labor stage in intervention group were significant lower than those in control group(P＜0.05).the rate of spontaneous delivery in intervention group was higher than that in control group(P＜0.05),the others had no significant difference between the two groups(P＞0.05).Conclusions Phloroglueinol and diazepam can decurtate the length of labor stage and lessen pain and have no effects on both mother and newborn.It is worthwhile to spread the drugsin clinical practice.

Objective To construct the recombinant X-linked inhibitor of apoptosis protein(XIAP) gene 3′untranslational region (3′UTR)-luciferase reporter vector ,and analyze the microRNA(miRNA) which possibly regulate the expression of XIAP gene . Methods Polymerase chain reaction (PCR) was employed to amplify X IA P-3′UTR sequences from human cDNA ,in which luciferase reporter vector pGL3-Ctrl was inserted ,and the recombinant vector pGL3-Ctrl/XIAP was gained .Target Scan 6 .2 soft-ware was adopted to predict the miRNA which possibly combined with the X IA P-3′UTR .pGL3-Ctrl/XIAP recombinant plasmids and the miRNA were co-transfected into A549 cells ,and the X IA P-3′UTR-luciferase activity was measured .Results Confirmed by digestion and DNA sequencing ,the X IA P-3′UTR-luciferase reporter recombinant was successfully constructed .Prediction of miRNA target sites indicated that X IA P gene may be the target of miR-200b ,miR-200c and miR-429 .Compared with miRNA mim-ic ctrl group ,miR-200b ,miR-200c and miR-429 significantly reduced the luciferase activity of pGL 3-Ctrl/XIAP with statistically significant difference(P<0 .05) .Conclusion X IA P-3′UTR-luciferase reporter vector is successfully constructed .miR-200b ,miR-200c and miR-429 can obviously decrease the luciferase activity .