Objective To evaluate the characteristics of three blood sampling method in quails. Methods 300 healthy 5-week old male quails were randomly divided into 3 groups: blood samples were taken from the wing root vein, the left jugular vein, and the right jugular vein, respectively, 100 quails in each group. The advantages and disadvantages of the three methods of blood sampling were compared, including the difficulty of blood collecting, the quality of blood serum obtained, the amount of bleeding after venepuncture, and the general condition of the quails after blood collection. Results All quails survived well. The right jugular vein was relatively small, and the quality of obtained serum was good, the bleeding amount after blood collection was small, the wound was easy to recover, and the general condition of the quails was good. The left jugular vein method was a little bit more difficult to collect blood, the quality of the obtained serum was good, the bleeding amount of quails after blood collection was larger, and the general state post puncture was rather good. The blood collection from the wing root vein was relatively difficult, with more massive bleeding after the vein puncture, therefore, is not suitable for large amount blood collection. Conclusions Blood samples in a large amount can be collected from both the right or left jugular veins. Small quantity of blood samples can be collected by either one of the three method.