Objective To observe the clinical effect of ultrasound-guided technology in the operation of upper limb in patients with nerve block. Methods Two huandred patients had be performed upper limb operation in our hospital form Jan2012 to Dec 2013 were randomly divided into two groups, 100 cases of each group, group A who was guided by tra-ditional anatomical method;group B who was guided by ultrasound-guided positioning technolog, 1% Lignocaine +0.375%Ropivacaine+10 mg Dexamethasone were injected in two groups. Comparison of the effect of two groups nerve block: the onset time of anesthesia, success rate, the amount, complications. Results Anesthesia operation completion time, group B was shorter than group A (TA=7.7±1.9min, TB=4.6±1.4min);Nerve block work time, group B was longer than groupA(TA=19.4±3.4min, TB=10.7±2.3min);group B anesthesia effect was good, with excellence rate reached 97%and fine rate was 100.00%;group A anesthesia effect was general, excellence rate reached 47.00%,fine rate was 91.00%;group B adverse reactions incidence was 2.00%, group A was up to 9.00%;compared the above group A and B related datas of difference was statistically significant (P<0.05).The duration of anesthesia (TA=417.5±98.7min, TB=406.3±79.1min), the more similar between two groups had no statistical significance (P>0.05). Conclusion Ultrasound-guided technology was applied to the upper limb brachial plexus block, which could enhance the effect of nesthesia, re-duce the dosage of anesthetic drug ,complications and adverse reaction.