OBJECTIVETo study the effects of transection of inferior alveolar nerve (IAN) on the healing of mandibular fracture by investigating the changes of inflammatory factors expression, including tumor necrosis factor-alpha (TNF-alpha), interleukin-1 (IL-1) and interleukin-6 (IL-6) in the denervated rabbits' bony callus.

METHODS50 Chinese rabbits were randomly divided into the experimental group and control group, the experimental group underwent mandibular fracture and transection of IAN and the control group underwent mandibular fracture only. The bony callus were collected at 1, 2, 3, 5 and 7 d after operation, respectively. The expression of TNF-alpha, IL-1 and IL-6 in callus was detected by enzyme linked immunosorbent assay (ELISA), and the mRNA of TNF-alpha was detected by reverse transcription-polymerase chain reaction(RT-PCR).

RESULTSIn the experimental group, at the 1st day after operation, the expression of TNF-alpha (75.1%), IL-1 (68.3%) and IL-6 (72.7%) was lower than that in control group (P < 0.05). At the 2nd day, the expression of IL-1 (75.5%) was still lower than that in control group, but the expression of TNF-alpha was higher than that in control group. In the next observation points, all inflammatory factors decreased, and there was no difference between experimental and control groups. In RT-PCR, the detection of TNF-alpha mRNA in the experimental group was lower than that in control group.

CONCLUSIONIn the haematoma of mandibular fracture healing, transection of IAN causes neurohumor decrease, depression and delay of inflammatory reaction. It is unfavorable to fracture healing.

Animals ; Hematoma ; Interleukin-6 ; Mandible ; Mandibular Nerve ; RNA, Messenger ; Rabbits ; Tumor Necrosis Factor-alpha

Objective To investigate the safety,efficacy and feasibility of endovascular recanalization in non-acute symptomatic occlusion of the ophthalmic segment of internal carotid artery,and analyze the factors affecting its success.Methods Fifty-four patients with non-acute symptomatic occlusion of the ophthalmic segment of internal carotid artery,underwent endovascular recanalization in our hospital from January 2013 to December 2017,were enrolled.Modified Rankin scale (mRS) and National institutes of health stroke scale (NIHSS) scores were compared before and after treatment;the prognosis results were analyzed after 2 years of follow-up.Independent factors influencing the prognoses were identified by Logistic regression analysis.Results Recanalization was achieved in 52 patients and failure was noted in two patients.The mRS scores and NIHSS scores before treatment (2.25±0.13,18.43±1.36) were significantly higher than those after treatment (1.44±0.05,11.81±0.71,P＜0.05).After 2 years of follow-up,good prognosis was noted in 42 patients,poor prognosis in 8 patients,and loss of follow-up in 2 patients.Multivariate Logistic analysis showed that history of hypertension and history of diabetes were independent risk factors for the prognoses of patients with non-acute symptomatic occlusion of the ophthalmic segment of internal carotid artery after endovascular reeanalization (OR=1.429,95CI:1.146-1.783,P=0.005;OR=2.597,95CI:2.364-2.854,P=0.005).Conclusion The curative effect of interventional therapy for non-acute occlusion of symptomatic internal carotid artery is reliable,and histories of hypertension and diabetes can affect the prognoses of patients.