Objective To detect the genetic association between cirrhosis and polymorphism of IL-12B gene .Methods Observed in a sample of 173 parent/offspring trios where the proband net for cirrhosis using correlation analysis and haplotype relative risk a-nalysis .The polymorphism of rs15677380 ,rs14050311 of IL-12B gene was detected with PCR methods and SNP typing in all nucle-us families .Results The rs15677380 allele was connected with cirrhosis(P=0 .009) .Allele G was protect factor(Z= -2 .36) and allele A was the hazard factor(Z=2 .36) .The rs14050311 allele was connected with cirrhosis(P=0 .013) .Allele T was protect fac-tor(Z= -2 .24) and allele C was the hazard factor (Z=2 .24) .The haplotypes of G/T and A/C in the rs15677380 ,rs14050311 were associated with cirrhosis (P=0 .021 ,0 .015 ,Z= -1 .85、2 .16) .Conclusion It shows an association between cirrhosis and the poly-morphism of IL-12B gene in Chinese .

Objective To ivevstigate the feasibility, the effects and the therapy of peritoneal dialysis(PD) on severe acute pancreatitis (SAP). Method From January,2001 to Jaly 2006,48 patients of SAP were divided randomly into PD group and non-PD group in Qingzhou hospital. Both groups were treated by the conventional mode of therapy. In PD group , using the concept of PD,24 patients of SAP were treated with PD and NPD group were treated only with common therapy. The release time of abdominal pain and distention, CT scores, APACHE II scores, the time of hospital stay, cost of treatment in hospital, operative rate and rate of complications and recovered rate of the two groups were compared. Simutaneously. the concentration of serum and fluid filtrated inflammatory cytokines TNFα,IL-6,IL-8,IL-10 and CRP were also determined pro and post the therapy. Results In the PD and NPD group, the duration for disappear-ance of abdominal pain and tenderness,and amelioration for abdominal distension was (19.70 ±7.32) hvs. (81.46±36.68) h and (23.16±6.95) h vs.(78.19± 29.26) h;So that the PD group was precede to that in NPD group ( P < 0.05). The concentration of serum and fluid filtrated pro-inflammatory cytokines TNFα, IL-6, IL-8 and CRP at each observation points after PD was decreased significantly (P <0.05) in the PD group. But the concentration of the serum and fluid filtrated anti-inflammatory cytokines IL-10 was increased significantly (P < 0.05) as compared with that of the NPD group. Conclusions Through PD, the imbalance of pro-inflammatory and anti-inflammatory cytokines has been corrected at early stage of SAP. But the PD is method that is easy, quick, small wou-ndell,few complicated and effective in patients with early-phase SAP, and will be of great value in wide application and further study.

Objective To study the relationship between the polymorphism of interleukin-12B (IL-12B)gene and coronary heart disease.Methods We recruited 256 patients with coronary heart disease admitted to our department as the study group and 256 normal subjects as the control group.The polymorphism of IL-12B gene was detected by polymerase chain reaction and single nucleotide polymorphism.Coronary artery stenosis,visfatin,high sensitive C reactive protein and cardiac function were determined.Results The difference in rs15677380 and rs14050311 allele frequencies between the study group and the control group was significant (χ2 =6.19,7.24,P=0.045,0.021).The G allele of rs15677380 and C allele of rs14050311 were risk factors for coronary heart disease (OR=1.32,1.49).Conclusion IL-12B gene is associated with the occurrence and development of carotid atherosclerosis and participates in the development of coronary heart disease.

ObjectiveTo explore the characteristics of noncompaction ventricular myocardium under ultrasonic cardiography. Methods8 patients, 1 with non-symptom and other 7 with various cardiac dysfunctions and arrhythmias, accepted ultrasonic cardiography. ResultsNumerous ventricular trabeculae and deep intertrabecular recesses, as well as left ventricular dilatations were found under ultrasonic cardiography.ConclusionNoncompaction ventricular myocardium can be diagnosed with ultrasonic cardiography reliablely.

Objective To evaluate the predictive values of interleukin (IL)-2 and soluble interleukin 2 receptor (sIL-2R) in the serum and cerebrospinal fluid in early intracranial infection,and provide the reference for choosing diagnostic markers of early intracranial infection after craniotomy.Methods From January 2014 to January 2016,36 patients with intracranial infection after craniotomy in our hospital were chosen as infection group,and 45 patients without intracranial infection were as non-infection group.The body temperature and levels ofcerebrospinaI fluid glucose,blood white blood cell (WBC),cerebrospinal fluid WBC,serum IL-2,cerebrospinal fluid IL-2,serum sIL-2R and cerebrospinal fluid sIL-2R were compared between the two groups.The diagnosis values of IL-2 and sIL-2R in serum and cerebrospinal fluid in intracranial infection were analyzed by receiver operating characteristic curve (ROC) curve.The sensitivity and specificity of IL-2 and sIL-2R in the serum and cerebrospinal fluid in intracranial infection were compared between the two groups.Results The body temperature of the two groups showed no statistical difference (P＞0.05).As compared with those in the non-infection group,the glucose of cerebrospinal fluid in the infection group significantly decreased (P＜0.05),the blood WBC,cerebrospinal fluid WBC,serum IL-2,cerebrospinal fluid IL-2,serum sIL-2R and cerebrospinal fluid sIL-2R in the infection group significantly increased (P＜0.05).The areas under of curve for body temperature,glucose of cerebrospinal fluid,blood WBC,cerebrospinal fluid WBC,serum IL-2,cerebrospinal fluid IL-2,serum sIL-2R and cerebrospinal fluid sIL-2R,respectively,were 0.671,0.718,0.698,0.741,0.714,0.927,0.722 and 0.968;the sensitivity of those indexes,respectively,was 61.1％,63.9％,69.4％,91.7％,88.9％,91.7％,86.1％and94.4％;the specificityofthoseindexes,respectively,was 48.9％,82.2％,60.0％,55.6％,62.2％,88.9％,66.7％ and 91.1％.Conclusion The IL-2 and sIL-2R levels in serum and cerebrospinal fluid have decided values in diagnosis of intracranial infection after craniotomy,but the sensitivity and specificity of IL-2 and sIL-2R in cerebrospinal fluid are higher than others.