Objective To prepare the schizophrenia mouse model by using peritoneal injection of dizocilpine maleate(MK‐801) , then to study the peripheral blood miRNA‐132 abnormal expression in schizophrenia mouse model .Methods Adult male Kunming mice(KM) were selected and randomly divided into the normal saline group(control group) and MK‐801 group(experimental group) .The behaviors changes were observed .Real‐time PCR method was used to detect the miRNA‐132 expression in peripheral blood .Results (1) Compared with the control group ,the stereotyped behavior score in the experimental group was increased sig‐nificantly(P<0 .05) ,the spontaneous activity was increased significantly(P<0 .05) ,showing that the up‐right number was signifi‐cantly decreased ,while the walking‐grid number was significantly increased(P< 0 .05) ,which was consistent with the manifesta‐tions of schizophrenia mouse model .(2)Compared with the control group ,the miRNA‐132 expressed in the experimental group was down‐regulated ,the difference was statistically significant(P<0 .05) .Conclusion miRNA‐132 is abnormally expressed in blood of schizophrenia mouse .

Baby body plethysmography is a well-established technique of lung function determination,which is widely used to access the respiratory development and obstructive airway diseases by precisely measuring tidal breathing,functional residual capacity,and air way resistance.Babies who accept lung function measurements in the baby body plethysmography are under quiet state of spontaneous breathing without any subjective initiative,which results in multiplexed data acquisition,high precision and good repeatability.Clinical application of baby body plethysmography differs in different countries and areas,with the most intensive application in the German-speaking countries.In China,the clinical use of baby body plethysmography still remains in primary stage.Great progress has been achieved in its clinical use during the past 20 years,but there is still a lack of consensus on normal reference values or equations with which to interpret results,which has caused some trouble to diagnosis and identification of respiratory disease.In order to identify the nature and severity of airway disease,it is essential to understand the normal range of plethysmographic values that may occur in healthy infants of similar age,gender and body size.

Sinonasal inverted papilloma (SNIP) is one of the most common benign sinonasal tumors demonstrating a high recurrence rate after surgery, and sometimes presents malignant tendency or coexists squamous cell carcinoma. Therefore, it is essential to achieve complete surgical resection, especially the original location of SNIP. In this paper, we evaluated the predictability of preoperative CT and magnetic resonance imaging (MRI) for localization of SNIP origin.
Carcinoma, Squamous Cell ; diagnosis ; pathology ; surgery ; Humans ; Magnetic Resonance Imaging ; Nose Neoplasms ; diagnosis ; pathology ; surgery ; Papilloma, Inverted ; Paranasal Sinus Neoplasms ; diagnosis ; pathology ; surgery ; Prognosis ; Tomography, X-Ray Computed

Objective To investigate the influence of risperidone on differentiation of 3T3‐L1 pre‐adipocytes .Methods 3T3‐L1 pre‐adipocytes were induced to differentiate into mature adipocytes by adopting the classic hormone cocktail method and observed by the oil red O staining .Meanwhile ,the inducing medium was added with risperidone for studying its influence on 3T3‐L1 pre‐adi‐pocytes differentiation .Results 3T3‐L1 pre‐adipocytes were successfully differentiated into the mature adipocytes ,0 .1 ,1 ,10μmol/L risperidone all could inhibit the differentiation of 3T3‐L1 pre‐adipocytes .Conclusion Risperidone can inhibit the differentiation of 3T3‐L1 pre‐adipocytes .

Objective To access the changes of lung function of newborns with pneumonia and to investigate the feasibility of measuring lung function in the body plcthysmograph for diagnosing and treating respiratory diseases.Methods There were 117 infants,1 day after being diagnosed with mild or moderate pneumonia,aged 4-28 days,involved in the study,those who needed oxygen cure,mechanical ventilation,or had complications from pneumonia were ruled out.Pulmonary function tests (PFTs) were performed in the baby body plethysmography to access tidal breathing,plethysmographic functional residual capacity (FRCp),and effective airway resistance (Reff).The other 126 contemporaneous healthy children took the same test for comparison.According to their ages,infants were grouped into 4-7 d,8-14 d,15-21 d and 22-28 d.Results Of the 117 infants with pneumonia,79 (67.5 ％ equivalently) had abnormal lung function.Compared with the controls,infants with pneumonia had significantly higher values of peak tidal expiratory flow [(59.4 ± 16.6) mL/s vs (52.9 ± 16.6) mL/s],expiratory flow at 75％ tidal volume [(57.4 ± 16.7) mL/s vs (49.5 ± 16.7) mL/s],special effective airway resistance (0.48 kPa · s vs O.27 kPa · s),Reff [6.96 kPa · s/L vs 4.82 kPa · s/L] and Reff per kilogram [1.99 kPa · s/(L · kg) vs 1.44 kPa · s/(L · kg)],but lower values of time to peak tidal expiratory flow as a percentage of total expiratory time [(26.5 ± 10.0) ％ vs (36.8 ± 9.9) ％],volume to peak tidal expiratory flow as a percentage of total expiratory volune [(27.9 ± 7.3) ％ vs (35.6 ± 8.4) ％]and expiratory flow at 25％ tidal volume [(36.3 ± 11.7) mL/s vs (40.1 ± 12.4) mL/s].There were no sig± nificant differences in the values of respiratory rate,FRCp and FRCp/kg between the pneumonia children and the healthy control chidren.Compared with the infants aged ≤ 14 days,there were more significant differences in relative parameters of lung funetion between the pneumonia group and the healthy control group for infants aged 15-28 days.Conclusions Newborns with pneumonia had a high ratio of abnormal lung function,which went up to 67.5％,and had apparent obstruction of small airways.Neonates with pneumonia aged 15-28 days had more apparent lesion of lang function.Measuring lang function by means of plethysmography among newborns is feasible and it can detect abnormal lung function sensitively.

OBJECTIVE:To study the antiulcer action of norfloxacin zinc(NF-Zn) METHODS:Acute and chronic gastric ulcer models were established in rats and the in vitro action of neutralizing hydrochloric acid and the MIC for Hp of NF-Zn were determined RESULTS:NF-Zn could inhibit the formation of acute gastric ulcer induced by ethanol,stress and salicylic acid in rats,accelerate the healing of chronic gastric ulcer produced by acetic acid,inhibit the secretion of gastric acid and increase the pH in gastric juice In vitro,NF-Zn could neutralize HCl and inhibit Hp with MIC50 of 0 5?g/ml CONCLUSION:The results suggest that the antiulcer action of NF-Zn may be related with its protection of the gastric mucosal barrier,neutralization of acid and inhibition of Hp

Objective To investigate the pollution condition of Salmonella in food with phage. Methods Salmonella in 413 samples of food were detected by the diagnosis of Salmonella phage with biochemical and serological identification. Results 119 Salmonella were detected in 99 positive samples and the isolating rate was 24%. Conclusion The disservice of Salmonella is mainly from the meat food and eggs. The detection method of phage is fast,convenient and reliable.

Object To observe the protective effect of total isoflavones of pueraria DC (TIP) on osteoporosis induced by estrogen deficient rat Methods 42 10 month old female SD rats were bilaterally ovariectomized and 10 animals were made sham operation (Sham) under anesthesia. After 7 d, the ovariectomized rats were divided into 4 groups: untreated control (OVX) group (n=12); 2 treated groups (n= 10 each), one with 100 mg/kg TIP ig and the other with 20 mg/kg TIP ig, and a niylestrol group (E 3) (n= 10) The total body bone mineral content (BMC), and bone mineral density (BMD) and bone biodynamics were detected at 4th and 7th month Results 1 Compared with OVX group, BMC and BMD in TIP 100 mg/kg group were increased by 14 6% and 12.1% at 4th month, by 6 8% and 14 2% at 7th month, while in TIP 20 mg/kg group by 7 8% and 6 9% at 4th month, by 8 6% and 14 9% at 7th month 2 The densities of femur in TIP 100 and 20 mg/kg groups were respectively increased by 3 8% and 3 4%, while those of tibia by 6 8% and 6 3% than that of OVX control group The Ca contents were increased by more than 15% in the TIP treated groups 3 The maximum load and structural strength of femur were increased by 16 1% and 19 4% in TIP 100 mg/kg group and 9 1% and 12 5% in TIP 20 mg/kg group than OVX control group These parameters in tibia were also improved 4 Uterine weights both in TIP 100 and 20 mg/kg groups were almost increased by one fold than OVX group Conclusion TIP can protect osteoporosis induced by ovariectomized rats and these effect may be attributable to its week estrogen like action

Objective To assess the clinical efficacy of acupuncture at the sphenopalatine ganglion for the treatment of patients with moderate to severe allergic rhinitis.Methods 42 cases of moderate to severe allergic rhinitis patients in Otorhinolaryngology clinic of Tongren Hospital between August 2012 to November 2013 were randomized into a treatment group and a control group. The treatment group was treated by acupuncturing sphenopalatine ganglion, once per week, altogether 4 times. The control group was treated by acupuncturingFengchi(GB20),Baihui(GV20),Yingxiang(GV29),Shangxing(GV23),Taiyang(EX-HN5), Quchi(LI11) andZusanli (ST36) was used in the control group, 3 times per week for 4 weeks. The changes of the Total Nasal Symptoms Score (TNSS) and the Total Non-Nasal Symptom Score (TNNSS) were evaluated before and after treatment. The Rhinoconjunctivitis Quality of Life Questionnaire (RQLQ) was used to evaluate the quality of life.Results One week after treatment, the scores of TNSS, TNNSS and RQLQ in both groups were significantly improved than before the treatment (treatment group: 5.0[4.0, 6.0]vs. 9.0[7.0, 10.0], 1.0[1.0, 1.5]vs. 3.0[3.0, 3.0], 44.0[35.5, 50.0]vs. 60.0[53.5,75.0]; control group: 6.0[5.5, 8.0]vs. 10.0[8.5, 10.1], 2.0[2.0, 3.0]vs. 3.00[3.0, 3.0], 50.0[45.5, 58.0]vs.43.0[27.0, 48.0]; allP<0.01); the scores of TNSS, TNNSS and RQLQ 1 week after treatment in the treatment group were significantly improved than those in the control group. The scores of TNSS(2.0[1.0, 3.0]vs. 3.0[2.0, 4.5]), TNNSS(1.0[0.0, 1.0]vs.2.0[2.0, 3.0]) and RQLQ(24.0[19.0, 29.0]vs. 43.0[27.0, 48.5])4 week after treatment in the treatment group were significantly improved than those in the control group(allP<0.01).Conclusions Acupuncture at the sphenopalatine ganglion could relieve the symptoms and improve the quality of life in patients with moderate to severe allergic rhinitis, and show more rapid and persistent therapeutic effect. It may be superior to the common acupuncture.

Objective To introduce a novel technique of intracolonic shunt procedure used in the anus - preserving operation for acute intestinal obstruction resulted from cancer at low and middle portions of rectum and assess the clinical significance. Methods In total, 81 patients with acute obstruction of low and middle portion of rectum caused by cancer were randomly ( random number) divided into control group and study group. In control group, 42 patients were operated with preventive transverse colonostomy or terminal ileum stoma after low proximal resection of rectum involved in cancer, while 39 patients were operated with intracolonic shunt procedure by using a biodegradable anastomosis ring and a condom placed 5 cm above anastomosis for protection in study group. Results There were no significant differences in sex, age, tumor site, tumor size and the distance from anstomosis to anal-edge between two groups. In both groups, the bowel movement resumed in 2 ~ 5 days after operation (P > 0.05). In study group, the rate of anastomosis leakage was 7.7% (3/39), and leakages were treated with drainage for 7.1 days in average to be healed, and the biodegradable anastomosis ring detached and were discharged in 14 -23 days (17 days in average), and there were no complications of drainage happened. The anastomotic stenosis occurred in three patients (7. 7% ) within 6 months after operation. In control group, 11.9% patients (5/42) had anastomosis leakage and they treated with drainage for 18.2 days in average to get the leakage healed, and 35. 7% patients (15/42) had stoma complications, and anastomotic stenosis happened in 28.6% patients (12/42) within 6 months after operation, and 7. 1% patients need another operation because of severe anastomosis stenosis. There were no significant differences in rate of anastomosis leakage between tow groups ( P > 0. 05), but there were significant differences in drainage days after anstomosis leakage happened and 6 - months anastomosis stenosis between two groups (P<0.05). Conclusions In the anus -preserving operation for acute intestinal obstruction at low and middle portions of rectum caused by cancer , the intracolonic shunt procedure is convenient and safty, and reduces the hazard incurred by anastomosis leakage and anastomosis stenosis compared with classic stoma operation.