OBJECTIVE:To investigate the influence and safety of etomidate or sevoflurane combined with sacral or epidural block anesthesia on anesthesia effects and inflammatory factors. METHODS:160 children undergoing surgery selected from our hospital during Feb. 2012 to Dec. 2015 were divided into group A and B according to random number table,with 80 cases in each group. Group A was given etomidate 3 mg/(kg·h)for anesthesia maintenance+sacral or epidural block;group B received sevoflu-rane inhalation 1%-3% for anesthesia maintenance+sacral or epidural block. The anesthesia effects were compared between 2 groups as well as the levels of serum S100β,NSE,Aβ and inflammatory factors before and after surgery. The occurrence of ADR was observed. RESULTS:There was no statistical significance in SpO2 between 2 groups before and after surgery(P>0.05). The anesthesia induction time,postoperative recovery time and heart rate of group A were significantly longer or higher than those of group B,with statistical significance(P<0.05). There was no statistical significance in the levels of serum S100β,NSE,Aβ and inflammatory factors between 2 groups before surgery(P>0.05). After surgery,the levels of serum S100β,NSE,Aβ,CRP,IL-2 and IL-6 were significantly increased in 2 groups,and group A was significantly higher than group B,with statistical significance (P<0.05);there was no statistical significance in TNF-α before and after surgery or between 2 groups (P>0.05). No obvious ADR was found in 2 groups. CONCLUSIONS:Sevoflurane combined with sacral or epidural block anesthesia is better than etomi-date combin ation anesthesia,with in mild inflammatory reaction and good safety.

The broad-spectrum antibacterial action of berberine hydrochloride mainly contributes to recurrent aphtha, periapical periodontitis, radioactive mucositis and pericoronitis, however a little evidence support the action mechanism underlying periodontitis treatment. OBJECTIVE: To determine the effects of berberine hydrochloride on the expressions of related cytokines in periodontal tissues of experimental periodontitis rats, to reveal and understand the action pathway of berberine hydrochloride on oral tissue repair. METHODS: Sixty Wistar rats weighing 160-200 g, aged 3 months Models, were involved in this study.Models of experimental periodontitis were established in rats through a use of local steel-wire ligation and systemic injection of prednisone acetate. Forty successfully established models were randomized into periodontitis model group (n=8) and periodontitis treatment group (n=32), at the same time, 10 normal rats served as control group. The treatment group of animals were fed with 0.06 g/kg berberine hydrochloride daily and medicated to death over the 1, 2, 3, 4 weekends (8 rats each). The model group was fed with isodose normal saline. The model group and normal control group were killed at the fourth weekend. Main observations: ①Oral gross observation and X-ray film examination; ②Pathological assay of periodontal tissues; ③Immunohistochemical SABC method was conducted to determine the expressions of tumor necrosis factor-α (TNF-α), bone gla protein (BGP), interleukin-1β (IL-1β), interleukin-6 (IL-6) in periodontal tissues in rats. RESULTS AND CONCLUSION:①Following hormone injection, gum tissue exhibited erosion and pyorrhea in model group of rats; the above-mentioned symptoms were relieved in rats of treatment group; there was no abnormality in periodontal tissues of normal rats. X-ray examination revealed alveolar crest resorption and obvious interredicular shadow in the model group.②Rats of model group showed obvious pathologic changes in periodontal tissues, the levels of TNF-α, IL-1β, IL-6 were significantly higher and the level of BGP was dramatically lower than those in normal group (P < 0.05); Treatment with berberine hydrochloride decreased the levels of TNF-α, IL-1β, IL-6 in periodontal tissues and increased the level of BGP compared with model group (P < 0.05). The periodontal tissues in groups treated with berberine hydrochloride exhibited pathological changes at inflammatory repair stage. Results showed that berberine hydrochloride inhibits the expressions of TNF-α, IL-1β, IL-6 in periodontal tissues in experiment rat models of periodontitis, and promotes the expression of BGP and repair of periodontal tissue.

Objective To investigate the combining anticancer effect of tamoxifen(TAM) and ?-interferon on breast cancer cells in vitro and its mechanism.Methods MCF-7 ER-positive breast cancer cell lines were treated with tamoxifen alone,or in combination with ?-interferon and/or estrogen in vitro.Cell proliferation was evaluated by MTT assay;FCM was used to determine the distribution of cell cycle,cell apoptosis and protein expression of Bcl-2,Bax,Fas,FasL,Caspase-8,and the activity of Caspase-3.Results TAM inhibited the proliferation of ER-postive breast cancer cells with cell cycle arrest in G0/G1 phase and with induction of apoptosis,and the proliferation-promoting effect of estrogen on MCF-7 was blocked by TAM.Anticancer effect of TAM was enhanced when cells were pretreated with ?-interferon for 24 hours.Bcl-2 protein expression was down-regulated and Caspase-8 was up-regulated by TAM and/or ?-interferon,but these drugs did not affect Bax,Fas,FasL protein expression and the activity of Caspase-3.Conclusions TAM has anticancer effect by inhibiting proliferation and inducing apoptosis in ER-positive breast cancer cells in vitro,and ?-interferon can enhance anticancer effect of TAM on breast cancer cells.The mechanism of these effects may be related with the down-regulation of Bcl-2 expression and up-regulation of Caspase-8 by TAM and ?-interferon.

AIM: To establish the fingerprint chromatogram of Dangguijisheng Injection (Radix Angelicae Sinensis, Herba Visci) METHODS: HPLC with Zorbax SB-C 18 ( 4.6mm? 250mm,5-Micro) column was used, A phase (mechanol∶tetrahydrofuran=85∶15) and B phase ( 0.5% acetic acid (gradient elution)) were adopted as a mobile phase, respectively, and detection wavelength set at 270 nm. RESULTS: 18 peaks constitued the HPLC fingerprint of Compound Dangguijisheng Injection. CONCLUSION:The method is simple and accurate with a good reproducibility and can be used as a quality control for Dangguijisheng Injection.

Cerebral Hemorrhage ; diagnosis ; Cerebral Infarction ; diagnosis ; Diagnosis, Differential ; Humans ; Medicine, Chinese Traditional

Objective To observe the effects of high sucrose,high saturated fatty acid and high unsaturated fatty acid(diets) on insulin resistance and endothelium-dependent vasodilation function.Methods Adult Wistar rats were divided into normal control(NC) group,high sucrose(HS) group and high saturated fatty acid(HSF) group,high unsaturated fatty acid(HUF) groups.Insulin sensitivity was tested by hyperinsulinemic-euglucemic clamp after 24 weeks.Acetylcholine-induced(or sodium nitroprussideinduced) relaxation of preconstricted isolated renal arteries was measured by Mulvany myograph.Results GIR was obviously lower in experimental groups than that in NC group.GIR was negatively correlated with triglyceride (TG),free fatty acid(FFA).Acetylcholine-induced relaxation was markedly decreased in all experimental groups compared with that in NC group and the maximal response was decreased 37.4% in HSF group,32.7% in HUF group,27.7% in HS group.Acetylcholine-induced relaxation was enhanced by incubation with L-Arg and decreased incubated with L-NNA,MB in all experimental groups.Vasodilation response was negatively correlated with TG,INS and well positively correlated with NO,GIR.There was significantly negative correlation between FFA andNO.Conclusions: The rats fed high sucrose,high saturated fatty acid and high unsaturated fatty acid diets developed insulin resistance with reduced endothelium-dependent vasodilation function.

Objective To explore the association between the polymorphisms of TSC1,TSC2,PTEN genes and autism in Chinese Han population.Methods 274 autism patients and 386 heahh controls were recruited,and SnaPshot technique was used to genotype the 13 tagSNPs of TSC1,TSC2 and PTEN genes.The allele,genotype and haplotype frequencies of the SNPs were compared using SHEsis and SNPStats softwares.Results Mter Bonferroni correction,the allele distribution of rs2809244 (TSC1) (x2 =9.537,P=0.002,adjusted P=0.016),rs1050700 (TSC1) (x2 =9.313,P=0.002,adjusted P=0.016),rs2072314(TSC2) (P＜0.01,adjusted P＜0.01) and rs8063461 (TSC2) (P＜0.01,adjusted P＜0.01)showed significant difference between two groups (P＜0.05).The genotype frequencies of rs2072314(TSC2)and rs8063461(TSC2) showed significant difference between two groups(P＜0.05).Moreover,the frequency of haplotype A-G (OR =14.548,95％ CI =5.450-38.830) in the haplotype block rs2809244-rs3761840 showed significant difference between two groups(P＜0.05),A-G significantly increases the risk of autism.The frequencies of haplotype A-A (OR=0.608,95％ CI =0.409-0.903,P=0.013),G-A (OR=7.812,95％ CI =5.338-11.459,P＜0.01)and G-G (OR=0.356,95％ CI =0.274-0.463,P＜0.01) in the haplotype block rs2074969-rs8063461 were identified,which were significant difference between two groups(P＜0.05),and AA and G-G significantly reduced but G-A increased the risk of autism.Conclusion The polymorphisms of TSC1 and TSC2 genes might associate with autism in Chinese Han population.

Objective To investigate the correlation between iodine intake,BRAF mutation in thyroid gland and clinical biologic characteristics in papillary thyroid carcinoma(PTC) cases.Methods A total of 159 PTC patients and 200 healthy controls were enrolled in this study.Urine iodine was tested,BRAFV600E mutation was detected by PCR.The correlation was analyzed between BRAF mutation and iodine intake,BRAF mutation and clinical biologic characteristics of PTC respectively.Results The median urinary iodine (MUI) of the patients and healthy controls was 336 μg/L and 196 μg/L respectively (P =0.004).The overall prevalence of the BRAFV600E mutation in this series of PTC was 63.5％,showing a clear correlation of BRAFV~E mutation with iodine intake (P =0.006).There was no correlation of BRAFV600E mutation with age,gender,tumor size,extrathyroid extension or nodulor goiter in PTC (P ＞ 0.05).But there was a significant association of BRAFV~E mutation with lymph node metastasis (P =0.008) and Hashimoto's thyroiditis (P =0.037).Conclusions High iodine intake may be a risk factor for PTC occurrence.In PTC cases,high iodine intake may be a risk factor of BRAFV600E mutation.BRAFV600E mutations increase both in PTC with cervical lymph node metastases and uncoexisting Hashimoto' s thyroiditis.

Objective To evaluate the changes in the levels of motilin in the duodenum in a rat model of incisional pain.Methods Eighty-four healthy male Sprague-Dawley rats,aged 6-8 months,weighing 180-220 g,were randomized into 2 groups (n =42 each) using a random number table:control group (group C) and incisional pain group (group P).The animals were anesthetized with sevoflurane.In group P,a 1 cm long incision was made in the plantar surface of right hindpaw.Six rats were chosen from each group and mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured at 24 h before operation (T0) and 1,6,24,48 and 72 h after operation (T1-5).Six rats were chosen from each group at T0-5 and sacrificed and the duodenal mucosal tissue was prepared for measurement of motilin levels by ELISA.Pearson linear correlate analysis was performed between the motilin level and pain threshold at each time point in group P.Results Compared with group C,MWT was significantly decreased,TWL was shortened,and motilin levels were significantly increased at T1-4,and no significant change was found at T0 and T5 in group P.The motilin levels were negatively correlated with MWT (r =-0.8 910) and TWL (r =-0.8 463) in group P.Conclusion Incisional pain can promote the secretion of motilin in the duodenum.

Aim To investigate the effect of resveratrol on proliferation and differentiation in K562 cells. Methods K562 cells were treated with different con-centrations of resveratrol for 6d. The colony number of K562 cells was detected with semi-solid culture assay. Expression of GATA-1 and PU. 1 in K562 cells was re-spectively measured with immunocytochemistry and Western blot. Expression of differentiation related anti-gen, CD11b, CD14 and CD42b, was measured with flowcytometry on K562 cells. Results Resveratrol
could significantly decrease the colony number of K562 cells in a dose-dependent manner, and enhance the ex-pression of GATA-1,PU. 1,CD11b, CD14 and CD42b in K562 cells. Conclusion Resveratrol could inhibit the proliferation and induce differentiation of K562 cells via up-regulating the expression of GATA-1 and PU. 1 protein.