1.Research on PEG modification of uricase
Lei CAI ; Xiangdong GAO ; Shu ZHU ; Hua WANG ; Wenbing YAO
Journal of China Pharmaceutical University 2008;(6):557-562
To modify uricase with PEG reagent in order to decrease uricase immunogenieity and increase its stability.Methods:The branched PEG of 40 kD was chosen to modify native uricase.The properties of the mod-ified uricase including the stabilities to protease,pH and temperature,in vivo half-life time,as well as the immu-nogenicity were evaluated.The pharmacokinetic profiles of the midofied uricase were studied in mice.Results:It is demonstrated that the conjugation of PEG to lysine residues of Candida utilis uricase resulted in higher tryp-sin resistance.reduced immune response.and prolonged in vivo half-life.PEG modified uricase retained 80% of the enzymatic activity of native uricase.In addition,it was found that half-life in serum of the intravenously injec-ted PEGylated uriease of up to 696 min was longer that that of native uficase of 45 min.Higher plasma drug con-centrations were also reached with dosing of the PEGylated uricase to mice.Furthermore,the binding affinity Was shown to be reduced for the PEG-uricase using ELISA assay.and it was one-eishth that of native uricase.Final-ly,it Was indicated that the PEG uficase induced a delayed immunoresponse in mice following repeated adminis-trations.Conclusion:These findings demonstrate that this chemically modified form of uricase may serve as a potentially effective drug to treat gout patients.
3.Surface marker changes in human umbilical cord-derived mesenchymal stem cells after cryopreservation and resuscitation
Yanxiang QIAN ; Qun SHU ; Hongxia CAI ; Juan GUO ; Liang CHEN ; Feng GAO
Chinese Journal of Tissue Engineering Research 2011;15(1):187-190
BACKGROUND: Mesenchymal stem cells are the stem cells that possess the capability for self-renewal and multi-directional differentiation. Umbilical cord is the tissue outside the embryos and would be fallen off after parturition. In addition, it has wide source and no ethical restriction, so it is promising to be the first choice for mesenchymal stem cells. OBJECTIVE: To detect the surface markers CD29, CD44, CD49e, CD73, CD90, CD34, CD45, and CD271 of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) prior to and after cryopreservation and resuscitation. METHODS: After isolation and culture, morphology of the primary, P4 and P8 hUCMSCs was observed prior to cryopreservation and after resuscitation. Surface markers CD29, CD44, CD49e, CD73, CD90, CD34, CD45, and CD271 of primary, P4, and P8 hUCMSCs were detected through the use of flow cytometry prior to cryopreservation and after resuscitation RESULTS AND CONCLUSION: hUCMSCs prior to cryopreservation and hUCMSCs of different passages after resuscitation present the same phenotype, i.e., positive for CD29, CD44, CD49e, CD73, and CD90, and negative for CD34, CD45, CD271. These findings suggest that primary hUCMSCs do not present changes in surface markers after cryopreservation and resuscitation.
4.Intraabdominal follicular dendritic cell sarcoma associated with leukocytosis: report of a case.
Dian-bin MU ; De-xian ZHANG ; Lin-ke YANG ; Shu-ping CAI ; Ju-jie SUN ; Yong-sheng GAO
Chinese Journal of Pathology 2013;42(5):349-350
Abdominal Neoplasms
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complications
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metabolism
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pathology
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surgery
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Adult
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Dendritic Cell Sarcoma, Follicular
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complications
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Female
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Humans
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Ki-1 Antigen
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metabolism
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Leukocytosis
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complications
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metabolism
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pathology
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surgery
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Receptors, Complement 3b
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metabolism
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Receptors, Complement 3d
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metabolism
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Young Adult
5.Clinical diagnosis and treatment of Meckel's diverticulum in children
Yan MA ; Zhigang GAO ; Lifeng ZHANG ; Yuebin ZHANG ; Tao PAN ; Duote CAI ; Qixing XIONG ; Qiang SHU ; Qingjiang CHEN
Chinese Journal of General Surgery 2017;32(8):674-677
Objective To evaluate the clinical characteristics and pathological features of Meckel's diverticulum(MD) in children.Methods 244 MD cases admitted between January 2010 and December 2014 were retropectively analyzed.Results In fifty patients,MD was an incidental finding at laparotomy or laparoscopy for unrelated entities.Among the remaining 194 symptomatic patients,there were 76 patients presenting GI bleeding,forty eight patients were identified with perforated Meckel's diverticulum,thirty six patients suffered from intestinal obstruction.34 patients had MD caused severe complications such as volvulus and intestinal necrosis,diverticular perforation and peritonitis.61 out of 76 GI bleeding patients underwent a 99mTc scan,and positive tracer was found in 42 patients.Among the 19 negative 99mTc scan patients,8 received capsule endoscopy and only 3 patients were suspected of diverticulum.242 patients underwent one stage resection of the diverticulum.Histology revealed ectopic gastric mucosa or ectopic pancreatic tissue in 128 patients.One patient died of volvulus and intestinal necrosis postoperatively,and two suffered from adhesive intestinal obstruction during one to five year's follow up.Conclusions It is necessary to maintain a high suspicion of MD in the pediatric age group with symptoms of abdominal pain,gastrointestinal hemorrhage or intestinal obstruction.Ectopic mucosa assumes the ultimate responsibility for major complications of MD.
6.Influence of the included angle between anterior aspects of S2 and S1 vertebral bodies on pelvic inlet imaging in mid-line sagittal plane.
Hong-ming CAI ; Shu-tu GAO ; Chuan-de CHENG ; Xue-jian WU ; Wu-chao WANG ; Jin-cheng TANG ; Shou-ya CHANG ; Wei-feng DUAN ; Chuan ZHANG
China Journal of Orthopaedics and Traumatology 2014;27(8):645-649
OBJECTIVETo analyze the influence of included angle between the anterior aspects of S2 and S vertebral bodies on pelvic inlet imaging in the pelvic midline sagittal plane.
METHODSTotally 58 axial pelvic CT scans were chosen as study objects including 43 males and 15 females,with an average age of 40.7 years old (ranged,18 to 68 years old). The angles between the anterior aspects of S2 and S1, vertebral bodies and the horizontal plane on midline sagittal CT reconstruction were measured to simulate the optimal S2 and S1 inlet angles. The included angle between the anterior aspects of S2 and S1 vertebral bodies was calculated by subtrocting the S1,inlet angle from the S2 inlet angle defined as a base number. Then, the impact of the calculated included angles on the pelvic inlet imaging was analyzed. Results:The S2 inlet angles averaged (30.5±6.5) degrees; the S inlet angles averaged (25.7±5.9) degrees. The difference between them was significant (t=3.35, P=0.001). Ten patients had zero angle between the anterior aspects of S2 and S1 vertebral bodies; 14 patients had negative angle, averaged-(8.9±8.1) degrees; 34 patients had positive angle,averaged (11.8+6.4) degrees.
CONCLUSIONThe difference of included angle between the anterior aspects of S2 and S1 vertebral bodies leads to the difference between S1 inlet view and S2 inlet view in most cases, complicating the pelvic inlet imaging,and affecting the reliability of the application of pelvic inlet view. Utilizing the angles measured on the preoperative midlihe sagittal CT reconstruction to obatin the patient-customized S1 and S2 inlet views could accurately guide the S1 and S2 iliosacral screw insertion.
Adolescent ; Adult ; Aged ; Animals ; Bone Screws ; Female ; Fracture Fixation, Internal ; methods ; Humans ; Image Processing, Computer-Assisted ; Male ; Middle Aged ; Pelvis ; anatomy & histology ; injuries ; Spine ; anatomy & histology ; Tomography, X-Ray Computed ; Young Adult
8.Chemical constituents from the fruits of Psidum littorale
qing Hang CUI ; ying Cai PENG ; zheng Ying HUANG ; Ying GAO ; qun Jian LIU ; Rui ZHANG ; cheng Ji SHU
Chinese Traditional Patent Medicine 2017;39(12):2538-2542
AIM To study the chemical constituents from the fruits of Psidum littorale Raddi.METHODS The ethyl acetate and methanol fractions of 90% ethanol extract from P.littorale were isolated and purified by silica,Sephadex LH-20,D-101 macroporous resin and preparative HPLC column,then the structures of obtained compounds were identified by spectral data.RESULTS Twelve compounds were isolated and identified as lupeol (1),betulin (2),nigaichigoside F1 (3),2oα-hydroxybetulinic acid (4),(-)-episyringaresinol (5),(+)-syringaresinol (6),(+)-isolariciresinol-9'-O-β-glucopyranoside (7),(7S,8R)-urolignoside (8),benzyl-β-D-glucopyranoside (9),butanedioic acid (10),β-sitosterol (11),daucosterol (12).CONCLUSION Compounds 1-9 are isolated from genus Psidium for the first time.
9.Observation on therapeutic effect of auricular point sticking combined with Tongshiji treatment on child ametropic amblyopia.
Chuan-Tong FEI ; Ying-Jie XU ; Shu-Qing XU ; Cai-ling GAO
Chinese Acupuncture & Moxibustion 2008;28(4):270-272
OBJECTIVETo compare therapeutic effects of auricular point sticking combined with Tongshiji treatment and simple Tongshiji treatment on child ametropic amblyopia.
METHODSThree hundred children of ametropic amblyopia were classified as mild, moderate and severe groups, 100 cases in each group. The each group was again randomly divided into a simple Tongshiji treatment (control group) and a auricular point sticking combined with Tongshiji treatment group (observation group). Their therapeutic effects were compared.
RESULTSThe total effective rate in the observation group were 100.0%, 79.8% and 71.0%, and in the control group were 100.0%, 54.3% and 48.2% respectivety for mild, moderate and severe groups. For the mild amblyopia children, there was no significant difference between the two groups in the therapeutic effect; for the moderate and severe ametropic amblyopia children, the therapeutic effect in the observation group was better than that in the control group (P<0.05).
CONCLUSIONAuricular point sticking combined with Tongshiji treatment for child ametropic amblyopia is of characteristics of convenient manipulation, obvious and rapid therapeutic effect.
Acupuncture, Ear ; Amblyopia ; therapy ; Child ; Child, Preschool ; Combined Modality Therapy ; Female ; Humans ; Male ; Medicine, Chinese Traditional
10.The study of the C5 nerve root palsy after surgery of cervical spondylosis.
Gao HE ; Jian-xiang ZHANG ; Cai-liang SHEN ; Qing-guo YANG ; Shu JIANG
Chinese Journal of Surgery 2005;43(12):781-783
OBJECTIVETo explore the clinical features, treatment and prognosis of the C5 palsy after surgery of cervical spondylosis.
METHODSTwo hundred and twenty-three cases treated from March 1994 to October 2003 were retrospectively reviewed.
RESULTSSeven of the 223 cases developed the complication of C5 palsy, manifesting the paresis of the deltoid muscle as well as the sensory deficits and (or) intractable pain in shoulder. The incidence was 3.1%. In this study, 2 cases occurred in the anterior subcorpectomy, 5 cases developed in the laminoplasty with 1 case on the opened side, 3 cases on the hinged side and 1 case on both sides. All the 7 cases with the C5 palsy recovered within 2 weeks to 6 months.
CONCLUSIONThe C5 palsy can develop either anterior decompression or posterior open-door laminoplasty of cervical spondylosis. Generally speaking, patients with postoperative C5 palsy can be cured by conservative measures. And prognosis is good.
Adult ; Aged ; Bone Transplantation ; adverse effects ; Cervical Vertebrae ; surgery ; Decompression, Surgical ; adverse effects ; Female ; Humans ; Laminectomy ; adverse effects ; Male ; Middle Aged ; Postoperative Complications ; diagnosis ; etiology ; prevention & control ; Radiculopathy ; diagnosis ; etiology ; prevention & control ; Retrospective Studies ; Spinal Nerve Roots ; Spinal Osteophytosis ; surgery