Objective To observe the clinical effect of patellar anaplasty and traditional knee arthroplasty in the treatment of osteoarthri-tis. Methods According to the operation method,50 patients with osteoarthritis were divided into patellar anaplasty group and knee arthro-plasty group,25 cases each. After surgery,all patients were followed up. Results The differences of the operation time,intraoperative blood loss,HSS score before and after operation,the WOMAC scores and radiographic evaluation between two groups were not statistically significant (P>0. 05). The complication rate of patellar anaplasty group was lower than those of knee arthroplasty group significantly,and the difference between two groups was statistically significant(P<0. 05). Conclusion Patellar anaplasty and traditional knee arthroplasty can improve the patient’s knee function effectively,and all the curative effect are reliable,but the complication rate of patellar anaplasty is lower,which is worthy of further promotion and research.

To investigate the prevalence and the antibiotic resistance of bacteria isolated from 25 miniature pigs. 45 bacterial strains were isolated, which were identified by biochemical assays, amplification of 16S rRNA genes by PCR and sequence analysis, and were evaluated for resistance to 30 antibiotics. The identification results showed that these bacteria belonged to Campylobacter (Campylobacter jejuni), Helicobacterium (Helicobacter pylori), Klebsiella (Klebsiella pneumoniae), Escherichia (Escherichia coli, Escherichia fergusonii), Pseudomonas (Pseudomonas aeruginosa), Stenotrophomonas (Stenotrophomonas maltophilia), Staphylococcus (Staphylococcus aureus, Staphylococcus haemolyticus, Staphylococcus simulans), Streptococcus (Streptococcus pneumoniae, Streptococcus suis, Streptococcus vestibularis, Streptococcus mitis, Gemella measles, Aerococcus viridans) and Bacillus (Bacillus subtilis, Bacillus licheniformis, Bacillus alvei, Bacterium megaterium). These bacteria were all susceptible to aztreonam and cephalothin. However, the resistence to furazolidone was found. Microbial population carried by miniature pigs in China had characters of diversity. Results of this study provided scientifical accordance for the microorganism monitoring of miniature pigs in China.

[Objective]To discuss the technique and outcome in the treatment of old nonunion of long bone fracture by using autologous fibula plate alone or combination with internal fixator plates.[Method]Fifty-seven patients(41 males and 16 females;average age of 41.6 years)with nonunion of long bone fracture were recruited.Of them 18 had short deformity(average 2.6 cm)and 12 had angled deformity(average 15?).Average time was 12 months(6-36 months).The sclerosed cortex was removed at both ends of the nonunion long bone.A suitable length of fibula plate were transplanted by screw with or without internal fixator plates.[Result]The follow-up average time was 3 years(ranged,1 to 6 years).At 1,3,6,12,24 months after operation,the outcome was analyzed by radiography.Bone healing was found in all 57 patients,with an average of 15 weeks(8-24 weeks).All the patients exhibited a good function in walking and burdening.No nonunion occurred.[Conclusion]Autologous fibula plate with or without internal fixator plates for nonunion of long bone fracture can facilitate healing and early functional recovery.This therapeutic method is an ideal technique in treating patients with nonunion of long bone fracture.

Objective To introduce the super minimally invasive transintercostal device closure of ventricular septal defect (VSD) and summarize it's therapeutic effect.Methods Nine patiens with VSD from August 2014 to December 2014 in our hospital were enrolled,which performed super minimally invasive transintercostal device closure of VSD.All patients were followed up prudently post-operation.Echocardiography was scheduled according to the protocol.Results All cases were successfully occluded.There was no death case in the peri-operation period and severe complications after operation.Conclusions The super minimally invasive transintercostal device closure of VSD is safe and reliable.

Objective This study was designed to study the effect of Diltiazem on patency rate of arteriovenous anastomosis in rat and how it works.Methods 24 SD rats were divided into control group and experimental group,12 rats in each group.Experimental group rats were gavaged with Diltiazem after vascular anastomosis.Control group rats were gavaged with water.By comparing the patency rate and the thickness of artery to make sure whether Diltiazem will affect the patency rate.;By comparing the clotting time,prothrombin time,artial thromboplastin time,and serum thromboxane B2 levels to explore the pathway of diltiazem.Results The patency rate was 75％ in the experimental group and 25％ in control group.Compared with the control group,experimental group venous blood vessels in the film segment was significantly thicker,clotting time was prolonged,TXB2 levels in blood was decreased,the differences were statistically significant(P ＜ 0.05).There were no significant difference in prothrombin time and partial thromboplastin between two groups (P ＞ 0.05).Conclusion Diltiazem can inhibit the secretion of TXB2,antagonize the effct of antiplatelet,and increase the patency rate of vascular anastomosis in rats.

Objective To investigate the protective effect of trichostatin-A (TSA) on cerebral ischemia/reperfusion injury via Janus kinase/signal transducer and activator of transcription (JAK/STAT) signal pathway.Methods 36 male SD rats were randomly (random number) divided into 3 groups:shamoperated group,ischemia/reperfusion (I/R) group and TSA group.Rat model of middle cerebral artery occlusion/reperfusion (MCAO) was established using a modified filament method.No occlusion was applicated to the sham-operated group.TSA group was injected with TSA 0.05 mg/kg via penile vein,20 minutes before operation.Reperfusion was carried out 24 hours after modeling.Longa 5 score was used to assess the neurological function,and TTC staining was applied to calculate the percentage of cerebral infarction area,The expression of JAK2 and p-JAK2 proteins was detected by Elisa.Results The low expression of JAK2 was observed in each group,and there was no statistical difference between groups (P =0.266).Compared with I/R group,TSA group had lower score in cerebral ischemia-reperfusion injury assessment (P=0.019),smaller area of cerebral infarction (P ＜0.01),reduced expression of p-JAK2 (P =0.009),all of which were of significant difference.Condusions TSA can reduce the cerebral ischemia/reperfusion injury via JAK/STAT signal pathway by down regulating p-JAK2 expression.

Objective To explore the correlation between obstructive sleep apnea hypoventilation syndrome (OSAHS)and glucose metabolism disorders in patients without diabetes mellitus.Methods A total of 88 patients with OSAHS but without diabetes mellitus from 2009 to 2011 in our hospital were selected and the pulse oximeter were used to measure the oxygen saturation.Patients were divided into 2 groups:the mild OSAHS group (n=46) and the severe OSAHS group (n=42).The age-and body mass index (BMI) matched control patients without OSAHS (control group,n =48) were randomly selected.The medical history,age,body height and BMI were recorded.The levels of fasting blood glucose,glycated hemoglobin (HbA1c),cholesterol,triglyceride and low density lipoprotein cholesterol were determined.Results There were significant differences in the numbers of respiratory disorders,respiratory disturbance index,average length of apnea,the longest apnea time,low-oxygen frequency,oxygen index,the time of oxygen saturation (SaO2) below 90％,minimum SaO2 and mean SaO2 between the OSAHS group and the control group (F=2.71,2.89,1.94,2.30,2.93,2.27,3.66,3.06,1.82,respectively,all P＜0.05).There were differences in the levels of fasting blood glucose,postprandial blood glucose,HbA1c,cholesterol,triglycerideand low density lipoprotein cholesterol between the three groups(F=1.81,1.85,2.16,1.77,2.24,2.19,respectively,all P＜0.05).Conclusions The severe degree of OSAHS has the correlation with the levels of glycated hemoglobin and blood glucose,which can provide a basis to observe the duration of diabetes mellitus and to predict the risk of cardiovascular diseases.

Objective To investigate the prevalence and risk factors of needle-sharing behavior among intravenous drug users (IDUs).Methods Data regarding socio-demographics,drug abuse characteristics and HIV related health literacy of 474 heroin IDUs were collected.Antisocial personality disorder of IDUs was diagnosed through Mini international neuropsychiatric interview.Results The prevalence of needle sharing among IDUs was 53.8％.Needle-sharing behavior of IDUs was significantly associated with male gender,marital status of being single and separated/divorced,local dwelling,an early age of first drug abuse and antisocial personality disorder (OR =1.11 ～ 6.69,P＜0.05).Conclusion Heroin IDUs have high prevalence of needle sharing.A comprehensive social,behavior and psychology based intervention is needed to effectively prevent HIV infection in IDUs.

Objective To study the immunoreaction of the recombinant proteins encoded by the fragments of ORF2 ( second open reading frames) gene of hepatitis E virus ( HEV). Methods The aimed sequence from the full-length ORF2 in clone PEH2 , which was derived from a Chinese strain of HEV,was amplified and cloned it into vector pcDNAS. 1 which was then transfected to 293 cell. Results The ORF2 protein was present in the soluble fractions of the cell lysate. The expressed protein of HEV ORF2 in 293 cells by using a plasmid pcDNA3. 1-based system showed positive on immunoblots probed against antibodies raised in BALB/c mice. Conclusion The experimental results laid a foundation for developing diagnostic reagent to detect HEV by using the expressed products of ORF2 protein.

Objective To evaluate the diagnostic value of Innovance D-dimer assay in patients with early thrombosis.Methods The D-dimer level was detected in 86 patients suspected with thrombosis and 40 healthy control subjects by using Innovance D-di-mer reagent.Results The D-dimer level was significantly higher in the case group[(4.14±7.29)mg/L]than in the control group [(0.17±0.12)mg/L],P =0.000.ROC curve analysis showed that D-dimer was a significant indicator for the clinical screening of thrombosis,and the higher D-dimer level,the greater possibility of thrombosis(AUC=0.938,P =0.000).The sensitivity,specifici-ty and negative predictive value have the satisfactory performance under the cut-off value of 0.50 mg/L.Conclusion The Innovance D-dimer is useful for excluding patients suspected with thrombosis,but it has a limited value in diagnosis of thrombosis.