Cardiomyopathy, Hypertrophic ; complications ; Fatal Outcome ; Heart Septal Defects, Atrial ; complications ; Humans ; Infant

Objective To evaluate the long-term effectiveness of different-dose of botulinum toxin A (BTXA) therapy on axillary hyperhidrosis.Methods Total 86 patients with axillary hyperhidrosis were self-controlled.One group of left axillary was injected with a low-dose of BTXA,50U.Another group of right axillary was injected with a high-dose of BTXA.A total dose of 200 U of BTXA was used per axilla.Patients were followed-up for 29 months.To investigate the effect of two methods,we analyzed two ranked data by rank sum test and x2 test to judge the disparities of the therapeutic effect.Results The results showed that the relapse-free interval of two groups with axillary hyperhidrosis was significant difference through the statistical analysis (P ＜ 0.05).Conclusions High-dose of BTXA treatment is capable of prolonging the antihidrotic effect on axillary hyperhidrosis.

Objective:To explore the relationship between tobacco smoking and liver cancer risk.Methods:Cohort studies of cigarette smoking and risk of liver cancer were identified by searching MEDLINE and CBM databases from January 1989 to September 2009. Results from individual studies were combined synthetically by Meta analysis using R software and Meta package. Results:A total of 19 articles were included in our analysis. The cohort totally included 3 023 558 followed-up persons and 9 525 cases of liver cancer patients. The pooled relative risk (RR) of liver cancer was 1.49 (95%CI: 1.17-1.92) for smokers compared with non-smokers. The results showed a statistically moderate association between tobacco smoking and the risk of liver cancer. Subgroup analysis indicated that the pooled RRs for smoking were 1.53(95%CI : 1.29-1.83)for male smokers and 1.70(95%CI :1.17-2.48)for female smokers, respectively.Conclusion:Tobacco smoking is a risk factor for liver cancer in both males and females.

[ ABSTRACT] AIM:To investigate the inhibitory effect of ginsenoside Re on intimal hyperplasia induced by bal-loon-injury and to explore the role of NF-κB p65 signaling pathway in the process.METHODS:SD rats (n=40) were di-vided into 5 groups randomly: sham operation group, model group, low-dose ginsenoside Re group, middle-dose ginsen-oside Re group and high-dose ginsenoside Re group.The carotid artery intima injury model was established by 2F balloon catheters in all groups except the sham operation group.The day after modeling, the animals in model group and sham op-eration group were administered intragastrically with distilled water, and the rats in low-dose, middle-dose and high-dose ginsenoside Re groups were given ginsenoside Re at doses of 12.5 mg/kg, 25mg/kg and 50 mg/kg, respectively.After 14 continuous days, the morphological changes of the injured arteries were observed by HE staining and the lumen area, intima area and media area as well as the ratio of intimal area/media area were determined.The expression of tumor necrosis fac-tor-α( TNF-α) and interleukin-1β( IL-1β) were detected by real-time PCR.The proliferating cell nuclear antigen ( PC-NA) and nuclear factor-kappa B ( NF-κB) p65 were examined by immunohistochemistry.RESULTS:Compared with sham operation group, the vessel cavity was narrowed (P<0.01), the mRNA levels of TNF-αand IL-1β, and the protein ex-pression of PCNA and NF-κB p65 were increased in model group (P<0.05).Compared with model group, the vascular intimal hyperplasia was alleviated obviously (P<0.05), and the mRNA levels of TNF-αand IL-1β, and protein expres-
sion of PCNA and NF-κB p65 were decreased in medium and high-dose ginsenoside Re groups (P<0.05).CONCLU-SION:Ginsenoside Re inhibits the vascular neointimal hyperplasia induced by balloon-injury in rats, and the molecular mechanism may be related to the inhibition of NF-κB p65 signaling pathway.

Objective:In order to improve the quality of standardized residency training in a tertiary hospital in Beijing, and to establish a multi-dimensional evaluation system based on post competency and professional accomplishment.Methods:The residents trained in our hospital were evaluated by questionnaires sent by third parties from the five aspects of evaluation by patients, peers, nurses, superiors and themselves. In this paper, SPSS 24.0 software was used to analyze the reliability and validity of the multidimensional evaluation scale. The evaluation results were analyzed by ANOVA and Pearson correlation analysis.Results:The hospital met the requirements for the post competency and professional accomplishment of the residents, and the overall level was good. However, the teamwork ability of some residents, especially the medical communication still needs to be improved.Conclusion:This study has enabled residents to pay more attention to the development of daily post competencies and professional accomplishment behaviors. It has provided an example basis for the further development of standardized residency training in the hospital, and carried out preliminary exploration for the design of training modules.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Spondylosis ; therapy ; Treatment Outcome ; Young Adult

Objective To investigate the effects of nerve growth factor (NGF) on the expression of 67-kDa laminin receptor (67LR) in human bile duct carcinoma QBC939 cells, and study the possible mechanism of perineural invasion and metastasis of bile duct carcinoma. Methods ( 1 ) The expression of a high-affinity receptor for NGF, TrkA, was detected by immunofluorescence staining. ( 2 ) QBC393 cells were pretreated by β-NGF at different concentrations ( 1, 10, 100,200 μg/L), and then the mRNA and protein expressions of 67LR were examined by Real-Time PCR and Western blot assay. QBC939 cells were divided into control group and β-NGF (1, 10, 100,200 μg/L) groups. (3) The ideal concentration of β-NGF was selected according to the results of previous tests, and then the mRNA and protein expressions of 67LR were re-examined by adding specific TrkA inhibitor K252a at different concentrations ( 100,200,300 nmol/L). QBC939 cells were divided into control group, β-NGF 100 μg/L group and K252a ( 100,200,300 nmol/L) groups. All data were analyzed by one-way analysis of variance or LSD-test. Results (1) A strong expression of TrkA was detected in the membrane of QBC939 cells. (2) The mRNA and protein expressions of 67LR in QBC939 cells were 0.35 ± 0.06 and 0. 32 ± 0.05 in the control group, 0.38 ±0.14 and 0.50 ±0.09 in the β-NGF 1 μg/L group, 0.62 ±0.14 and 0. 69 ±0. 13 in β-NGF 10 μg/L group, 0.90 ± 0.08 and 0.93 ± 0.07 in the β-NGF 100 μg/L group, and 0. 70 ± 0. 10 and 0. 76 ±0.07 in the β-NGF 200 μg/L group, there were significant differences among the five groups (F = 22. 4, 14. 6,P ＜0.05). The mRNA and protein expressions of 67LR in the β-NGF 100 μg/L group were significantly higher than those in the control group ( t = 19. 0, 21.0, P ＜ 0. 05 ). (3) The mRNA and protein expressions of 67LR in the QBC939 cells were 0.35 ±0.10 and 0.41 ±0.10 in the control group, 0. 88 ±0. 14 and 0.84 ±0.10 in the β-NGF 100 μg/L group, 0.80±0.08 and 0.76 ±0.04 in the K252a 100 nmol/L group, 0.67 ±0.12 and 0.61 ± 0.09 in the K252a 200 nmol/L group, and 0. 43 ± 0.07 and 0. 50 ± 0. 12 in the K252a 300 nmol/L group, there were significant differences among the five groups ( F = 14. 1, 8. 9, P ＜ 0.05 ). There were no significant differences in the mRNA and protein expressions of 67LR between the K252a 300 nmol/L group and the control group (t =1.02, 0. 85, P＞0.05). Conclusion In bile duct carcinoma cells, NGF enhances the expression of 67LR by combining with TrkA, which might be the mechanism of NGF mediating perineural invasion of bile duct carcinoma.

BACKGROUND:The establishment of animal models of Parkinson’s disease plays an important role in clinical and basic experiments of Parkinson’s disease, and its stability directly affected study results. OBJECTIVE:To evaluate the behavior and pathological changes of rats with Parkinson’s disease established by unilateral two-point injection with 6-hydroxydopamine.
METHODS:A total of 62 Sprague-Dawley rats were randomly assigned into experimental group (n=50) and normal group (n=12). Using stereotatic technique, 6-hydroxydopamine was injected into the right substantia nigra
RESULTS AND CONCLUSION:At 2 weeks, after induction with apomorphine, left rotation speed>7 r/min was observed in only 22 rats of the experimental group. No significant difference in the number of rotation between 2 and 4 weeks was detected (P>0.05). Tyrosine hydroxylase and dopamine content significantly reduced in the model group. Results indicated that unilateral two-point injection of 6-hydroxydopamine could be used to
successful y establish rat models of Parkinson’s disease with similar behaviors and pathologies.

Objective To analyse the mental health status of doctors in the First Affiliated Hospital of Xinjiang Medical University. Methods The 673 doctors in different clinic character in the First Affiliated Hospital of Xinjiang Medical University were investigated by questionnaire and symptom checklist-90(SCL-90),and estimate the results through F-test and t-test . Results Doctors in SCL-90 in addition to interpersonal relationships(1.59±0.49, t=-1.02,P>0.05) and the rest of the factors:somatization (1.48 ± 0.55, t=2.32), force(1.75 ± 0.43, t=2.34),depress(1.67 ± 0.44,t=3.00), anxiety(1.55 ± 0.57, t=3,71), hostility(1.76±0.47,t=5.65),horrible(1.48±0.32,t=6.35),stubborn(1.65±0.39, t=4.04),insanity(1.43±0.46, t=3.39)are higher than the national norm, there is statistical difference (P<0.05). In different gender groups, in addition to the somatization and forced, female doctors other project factors were higher than male doctors, including interpersonal relationships(1.62 ± 0.37, 1.56 ± 0.41), hostility factor(1.79 ± 0.38, 1.73 ± 0.43) differences were statistically significant (t=2.15, t=2.07, P均<0.05). In different professional title ,Doctors with primary professional title in addition to the body of the rest are higher than the title doctor. Anxiety factor differences have statistical significance in different groups (F=2.73, P<0.05). In different department , Emergency department and pediatric clinicians in addition to depression, anxiety and other factors of points were higher than those of physicians and surgeons, the difference in interpersonal relationship factors have statistical significance in different groups (F=4.54, P<0.05). Conclusion The scores aboutdoctors, SCL-90 in the First Affiliated Hospital of Xinjiang Medical University in addition to interpersonal relationships, the rest of the factors are higher than the national norm ,showing that clinic doctors were in psy chological sub health state, especially in female doctors, primary professional title dortors and Emergency department and pediatric clinicians.

Objective To study the influence of pulmonary ischemia-reperfusion (I/R) induced by cardio-pulmonary bypass (CPB) on pulmonary function and the preventive effect of nicardipine.Methods Sixteen patients scheduled for cardiac valve replacement were randomly divided into two groups : control group ( n = 8) and nicardipine group ( n = 8) . In nicardipine group nicardipine 0.02 mg kg-1 was given at the beginning of CPB; while in control group normal saline was given instead of nicardipine. All patients were operated upon under TIVA with large doses of fentanyl. Swan-Ganz catheter was placed via internal jugular vein after induction of anesthesia. Mean pulmonary arterial pressure (MPAP), pulmonary vascular resistance index (PVRI) and lung compliance were measured and calculated before CPB (T0 ), 5min after declamping of vena cava (T1 ), at tennination of CPB (T2) and at the end of operation (T3 ). At the same time points arterial and mixed venous blood samples were taken for determination of TNF-a, SOD and LPO concentrations and polymorphonuclear leukocyte (PMN) count, intrapulmonary PMN trapping (PMNa-PMNv) and blood gases and calculation of PaO2/FiO2 , P(A-2,)O2 difference and Qs/Qt. The vena cava cross-clamping time was defined as pulmonary ischemia time. Results (1) In control group MPAP, PVRI, PaO2/FiO2 and Qs/Qt were significantly deteriorating after vena cava declamping (T1-T3) as compared with the baseline valves (T0) (P