Objective To explore the correlation between Helicobacter pylori (H.pylori) infection and colorectal cancer.Methods From January 2008 to January 2014,263 patients pathologically diagnosed as colorectal cancer were selected as colorectal cancer group,and at the same period 263 patients with normal colon mucosa under colonoscopy were set as control group.The rates of H.pylori infection,the rates of H.pylori infection in patients with different pathological types of colorectal cancer,the rates of H.pylori infection accompanied with atrophic gastritis and the rates of H.pylori infection accompanied with intestinal metaplasia of two groups were retrospectively analyzed.The difference of H.pylori infection between proximal colorectal cancer and distal colorectal cancer was compared.Chi-square test was performed for comparison and odds ratio (OR) value and 95％ confidence interval (CI) were calculated.Results Among 263 patients with colorectal cancer,the H.pylori infection rate of colorectal cancer group was 63.50％ (167/263),which was higher than that of control group (39.54％,104/263),and the difference was statistically significant (x2 =27.66,OR=2.66,95％ CI 1.85 to 3.83,P＜0.01).There was no statistical difference in rates of H.pylori infection among different pathological types of colorectal cancer (x2=0.15,P=0.93).The rate of H.pylori infection accompanied with atrophic gastritis in patients with colorectal cancer was 46.39％ (122/263) which was higher than that of control group (23.57％,62/263),and the difference was statistically significant (x2=28.53,OR=2.94,95％CI 1.98 to 4.36,P＜0.01).The rates of H.pylori infection accompanied with intestinal metaplasia in patients with colorectal cancer was 17.87％ (47/263) which was higher than that of control group (4.18％,11/263),and the difference was statistically significant (x2=23.56,OR=5.50,95％CI 2.76 to 10.95,P＜0.01).There was no significant difference in the rate of H.pylori infection between the proximal colorectal cancer and the distal colorectal cancer (x2 =1.48,P =0.22).Conclusion H.pylori infection,together with atrophic gastritis and intestinal metaplasia caused by it are correlated with colorectal cancer.

Objective The morphological changes were observed in a pedigree with May-Hegglin anomaly cells.Methods Blood routine detection of abnormal May-Hegglin family members,peripheral blood cell Wright-Giemsa staining,and obser-vation of the white blood cell and platelet morphology.Made bone marrow smears of the proband,and megakaryocyte counts and classification.The proband peripheral blood leukocyte TEM examination were collected and analyzed by PCR sequen-cing.Results To reduce the May-Hegglin abnormal family blood platelet count in different degree,peripheral blood eosino-phil,eosinophilic inclusion visible form was not an alkaline cells,monocytes,neutrophils in body.Conclusion It’s provided valuable clues to diagnosis of morphological examination for May-Hegglin disorders.

BACKGROUND:The power output of the human joints depends on the activation of agonists and antagonist muscles around the joints, and the antagonist muscle is involved in physical activity in a co-activated manner, thereby helping to maintain joint stability. But there are less reports on the central nervous system strategies of antagonist co-activation under different muscle contraction modes. OBJECTIVE:To observe the characteristics and differences of surface electromyography (sEMG) of agonists and antagonist muscles of the knee joints during maximal and submaximal isokinetic exercises until fatigue. METHODS:Sixteen students were enroled, who developed the right knee joint fatigue induced by the Biodex isokinetic equipment, and simultaneously sEMG activities of vastus medialis, vastus lateralis and biceps femoris were recorded by the myoelectrical equipment. The RMS (%) and mean frequency were selected to analyze sEMG characteristics. RESULTS AND CONCLUSION:In the maximal isokinetic fatigue, the peak torque was decreased by 40.3% (P < 0.05) and the sEMG activity of agonists gradualy increased and then declined, but the sEMG activity of antagonists kept constant. In the submaximal isokinetic fatigue, the peak torque was decreased by 40.0% (P < 0.05), the sEMG activity of agonists gradualy increased (P < 0.05), and however the sEMG activity of the antagonists initialy increased and then decreased. The RMS and mean frequency of vastus medialis and lateralis as antagonists at selected reciprocal contractions showed no significant changes (P > 0.05). These results suggest that in the different intensities of isokinetic fatigue, the central nervous system employs different neural strategies on agonists and antagonists.

Adult ; Aged ; Alveolar Ridge Augmentation ; methods ; Dental Implantation ; methods ; Female ; Humans ; Male ; Maxillary Sinus ; surgery ; Middle Aged

Objective To study and analyze the efficacy and adverse reactions of beraprost sodium combined with aspirin in the treatment of diabetic foot.Methods 90 cases with diabetic foot were selected and they were randomly divided into the observation group and control group,35 cases in each group.Two groups were both treated with basic treatment.On the basis of the basic treatment,the control group was given aspirin,while the observation group was treated with beraprost sodium combined with aspirin.The clinical efficacy,ulcer healing rate,ankle back index,transcutaneous oxygen pressure,average healing time and the occurrence of adverse reactions were compared between the two groups.Results The clinical effective rate of the observation group was 93.3％ (42/45),which was significantly higher than 84.4％ (38/45) of the control group,the difference between the two groups was statistically significant (x2 =8.030,P ＜ 0.05).The wound healing rate,ankle dorsal index,average healing time,the degree of improvement of transcutaneous and oxygen pressuer of the observation group were (81.4 ± 2.7) ％,(11.2 ± 2.1),(39.1 ± 2.1) d and (26.3 ± 1.8) mmHg,which were significantly better than (69.3 ± 1.9) ％,(8.4 ± 2.9) ％,(52.3 ± 8.2) d and (16.3 ± 2.6)mmHg of the control group.The differences of these indicators between the two groups were statistically significant(t =12.72,7.92,12.06,9.97,P ＜ 0.05).The difference of adverse reactions between the two groups was not statistically significant (P ＞ 0.05).Conclusion Beraprost sodium combined with aspirin has better clinical efficacy in the treatment of diabetic foot.It can improve the patients'ulcer healing rate,the ankle back index,transcutaneous oxygen pressure,shorter the average healing time,and it has few adverse reactions.

Recent advancements on interventional molecular imaging aimed to further complement the advantages of two imaging fields, namely, interventional radiology and molecular imaging. Interventional molecular imaging significantly improved the early diag-nosis of cancer, local treatment, and monitoring of tumor treatments. Interventional radiology has continuously extended the capabili-ties of the currently available molecular imaging techniques to (i) obtain deep-seated targets;(ii) thoroughly examine small targets;(iii) precisely guide the delivery of non-targeted imaging tracers or therapeutics;and (iv) selectively enhance the effectiveness of targeted imaging and treatment with high accuracy. Molecular imaging has been used to guide interventional therapies and assess the thera-peutic efficacies of medical interventions. The continuous efforts on interventional molecular imaging extend molecular imaging from benches or small animal laboratories to large animal suites and ultimately to certain clinical applications in humans and enhance the diagnosis and treatment of cancer.

Objective To investigate articulation characteristics and rehabilitation training methods of abnor-mal supradental of patients with dysarthria after lingual frenectomy.Methods A total of 107 patients with dysar-thria after lingual frenectomy were studied for articulation characteristics.Then 32 patients with dysarthria were re-cruited from those 107 patients for further research of the training methods.Results The articulation disorders of/z/and/s/were replaced by/d/when/z/and/s/were combined with/a/,/o/,/e/,/u/or the vowels whose head vowels were them,/z/and/s/were replaced by/j/and/x/when they were combined with/i/.The proportion of these disorders were 32.71%,26.17%,30.84% and 40.19%,respectively.The articulation disorders of/c/was re-placed by/d/or/t/when/c/was combined with/a/,/o/,/e/,/u/or the vowels whose head vowels were them. The proportions were 5.61% and 25.23%,respectively.When/c/was replaced by/q/and/j/.When/c/was com-bined with/i/,the proportions respectively were 42.06% and 14.02%.Then/z/,/c/,/s/were totally replaced to/zh/,/ch/and ,/sh/or lateralization.The proportions of these disorders were 14.02%,15.89%,13.08% and 10.28%,9.35%,11.21%,respectively.After the speech training,the patients made less mistakes about supradental consonant,the number changed from 34.53±6.08 to 7.97±2.52.The differences were statistically significant(t=30.035,P<0.001).Conclusion The main articulation disorders were replacement and lateralization.The training methods that this research has established are significantly effective,and is worth receiving further study and possi-ble clinical application.

AIM: To clarify whether advanced glycation end products (AGEs) can influence the expression of mitochondrial fusion proteins Mfn1 and Mfn2 in cultured human aortic endothelial cells (HAECs) in vitro.METHODS:AGE-BSA was used as AGEs.Purchased primary human aortic endothelial cell line was multiplied, and transferred to dif-ferent passages for subsequent grouping.For dose-dependent experiment, HAECs were divided into 4 groups, and the con-centrations of AGE-BSA in each group were 0 mg/L (control group), 50 mg/L, 100 mg/L and 200 mg/L, respectively. For time-dependent experiment, HAECs were divided into 5 groups with the same concentration (100 mg/L) of AGE-BSA, but the intervention time was 0 h (control group), 6 h, 12 h, 24 h and 48 h, respectively.The mRNA and protein expres-sion levels of Mfn1 and Mfn2 in the HAECs were detected by real-time PCR and Western blot, respectively.RESULTS:Exposure of the HAECs to AGEs at different concentrations for 24 h all down-regulated the mRNA and protein expression levels of Mfn1 and Mfn2.Except for 6 h intervention group, 100 mg/L AGEs intervention for 12 h, 24 h and 48 h all down-regulated the mRNA and protein expression levels of Mfn1 and Mfn2 in cultured HAECs.CONCLUSION: AGEs down-regulates the expression of mitochondrial fusion proteins Mfn1 and Mfn2 in cultured HAECs, indicating that AGEs may influence mitochondrial dynamics of human aortic endothelial cells.

Three-dimensional virtual surgery experiment is the application of virtual reality technology in the medical field.Doctors use the information in the virtual environment to develop surgical planning,surgical drills.surgical teaching,surgical skills training,to lead the surgical technique and postoperative rehabilitation,and the information is available for surgery every sur-gical member to share.Virtual Surgery experiments combined with the real operation together constitute complete experimental teaching system,making telemedicine experimental teaching methods and teaching content rich and varied.

Objective To explore the changes of indicators for total parenteral nutrition (TPN) and parenteral nutrition (PN) combining enteral nutrition (EN) support in patients with severe acute pancreatitis (SAP), and to analyze the treatment effects of two groups. Methods 29 patients with SAP were randomly divided into two groups: TPN group (n=14) and PN+EN group (n=15). Nonoperative treatment were applied to the two groups. The changes of indicators and therapeutic outcome of two groups were recorded after nutrition support 14 days. Results After 14 days nutritional support, serum albumin (ALB), total serum protein (TSP), blood calcium, glutamic-pyruvic transaminase (ALT), glutamic-oxaloacetic transaminase (AST) in each group increased significantly (P<0.05). While blood glucose, serum amylase, leucocyte count decreased significantly (P<0.05). However, there were no significant difference between two groups except leucocyte count in all laboratory tests. The leucocyte count in the PN+EN group was significantly lower than that in the TPN group (P<0.05). In both group there was no difference found in serum total bilirubin (TB) before and after nutrition support (P>0.05). The APACHE II scores, average hospital days, average hospital charges, incidence of infection and mortality rate in the PN+EN group were significantly lower than that in the TPN group (P<0.05). ConclusionsPN combining EN support was superior to TPN in the treatment of patients with SAP.