Objective To study the correlation between human leukocyte antigen (HLA)-DRB1 alleles and anti neutrophil cytoplasmatic antibodies (ANCA) in Han and Uygur ulcerative colitis (UC)patients in Xinjiang region.Methods The serum ANCA was determined by indirect immunofluorescence assay in 62 Uygur UC patients,58 Han UC patients,188 Uygur and 184 Han healthy control individuals.HLA-DRB1 typing was performed by polymerase chain reaction-sequence based typing (PCR-SBT).The allele frequency of HLA-DRB1 was compared in ANCA positive and negative Han and Uygur patients as well as healthy controls.Stratified analysis was performed according to UC clinical type,severity and involvement.SPSS 17.0 software was applied for x2 test.Once P＜0.05,the odds ratio (OR) and 95％ confidence intervals (95％CI) was calculated.Results The positive rate of ANCA in Uygur UC patients (53.2％,33/62) was significantly higher than that of Han patients (34.5％,20/58) and the difference was statistically significant (x2=4.269,P =0.045).In Uygur,the gene frequency of HLA-DRB1 * 13 in ANCA positive UC patients (0.202)was significantly higher than that of ANCA negative patients (0.017) (x2 =10.092,P=0.016,OR=16.000,95％CI:2.892 to 88.524) and healthy controls (0.075) (x2=9.351,P＝0.040,OR=3.407,95％CI:1.666 to 6.971).The gene frequency of HLA-DRB1 * 13 in ANCA positive pancolitis type UC patients (9/15) was significantly higher than that of ANCA negative pancolitis type UC patients (1/14) and the difference was statistically significant (x2=8.955,P =0.040,OR =19.500,95％CI:2.787 to 136.461).However,in Han patients,there were no significant differences of HLA-DRB1 alleles frequencies among ANCA positive patients,ANCA negative patients and healthy controls (all P＞0.05),and the results of stratified analysis were same.Conclusions In Uygur UC patients of Xinjiang region,HLA-DRB1 * 13 may correlated with ANCA and with ANCA of pancolitis type UC patients.There is no such correlation in Han patients of Xinjiang region.

Objective To investigate the application of CT microvascular permeability surface (PS) in acute ischemic stroke. Methods Thirty patients suffering from acute or sub-acute cerebral infarction underwent CT perfusion (CTP) combining with CTA. Cerebral hemodynamic parameters (cerebral blood flow [CBF], cerebral blood volume [CBV], time to peak [TTP], PS) between lesions and contralateral side were compared. The manifestation of microvascular hyper permeability were analyzed. Results Significant differences were found in cerebral hemodynamic parameters between the core of cerebral infarction and the contralateral hemisphere (P＜0.05). There was no significant difference in CBF and CBV between the surrounding of cerebral infarction and the contralateral hemisphere, but PS and TTP showed delaying and heightening, the difference was significant (P＜0.05). High-density extravasation of contrast media in infarction zone was observed 30 min after CTP in 3 patients, the value of PS reached (9.20±1.43) ml/100 (ml·min). Conclusion CT microvascular PS may monitor hemorrhagic transformation in acute ischemic stroke, and guide thrombolytic therapy.

AIM:Cyclin is a decisive factor of regulating cell cycle,and RNA interference as an effective and specific gene silencing technique,can induce cell express the phenotype of specific gene deficiency. This study is to apply cyclinD1 specific small interfering RNA(siRNA) on inhibiting cyclinD1 gene expression and investigate the effect of cyclinD1 specific siRNA on the cell cycle and multiplication in keloid fibroblasts. METHODS:The experiment was conducted at the Institute of Genetic Engineering(Grade BSL-2) in Southern Medical University from July 2006 to May 2007.①siRNA was designed with siRNA target finder of ambion Company,and synthesized chemically in Shanghai GeneChem,Co.,Ltd. Then double-strand siRNA was obtained following degenerative renaturation. Keloid fibroblasts were sampled from the patients in the Department of Plastic Surgery,Southern Medical University(informed consents were obtained from the patients or their relatives).②The keloid fibroblasts were divided into experimental group and control group. The cyclinD1 specific siRNA was transfected into the keloid fibroblast of the experimental group by the liposome-mediated gene transfection method. The untreated cells were set as controls.③At hours 24,48 and 72 of transfection,light microscope was used to observe cell morphologic change and flow cytometry was used to examine cell cycle. The viable cells were counted by MTT colorimetry and a cell growth curve was drawn. RESULTS:①Abnormal change of cell morphology that became into spherical shape or oval-shape from normal long fusiform after transfection may be the apoptosis or necrosis cells.②The G1 stage of cell cycle extended and the S stage decurtated. At 24,48 and 72 hours after transfection,the radio of G1 stage cell was 60.13%,66.22% and 67.53%,which were all significantly higher than that in the control group(54.53%);the radio of S stage cell(18.25%,17.11% and 11.15%) was also significantly lower than that in the control group(22.31%),indicating that the proportion of the cells blocked in G1 stage and those in S stage decreased in the keloid fibroblast.③siRNA-cyclinD1 inhibited the growth of keloid fibroblasts obviously by using MTT assay,and the cell growth curves indicated that the proliferation of cell transfected with cyclinD1 specific siRNA was inhibited significantly when compared with controls. CONCLUSION:CyclinD1 specific siRNA effectively inhibits the expression of cyclinD1 in keloid fibroblasts thus arresting the cell cycle at G1 stage and enhancing cell apoptosis.

Objective To investigate serum levels of interleukin(IL)-22 in typeⅠhypersensitivity disease patients with monosensitization and polysensitization,inhaled allergens and food allergens,and explore the correlation between IL-22 levels and allergens.Methods A total of 100 patients with typeⅠhypersensitivity disease and 30 normal controls were recruited in this study.Western blot and enzyme-linked immuno sorbent assay were used to detect 19 types of allergens′ specific IgE antibody and IL-22 concentrations,respectively.Results Serum level of IL-22 was positive correlated with the number of sensitized allergens(r=0.318,P=0.001).The levels of IL-22 in polysensitization patients,monosensitization patients and normal controls were 24.52(20.41,29.27),22.02(15.25,25.59),18.06(16.02,23.04)pg/mL respectively.IL-22 in polysensitization patients(n=42) were higher than those in monosensitization patients(n=58) and normal controls(U=867.500 and 229.000,P<0.05,respectively).Compared with the normal controls,the IL-22 level was also higher in monosensitization patients(U=608.000),the difference was significant(P<0.05).However,there was no significant difference on IL-22 between inhaled allergens patients(n=34) and food allergens patients(n=24)(t=0.082,P>0.05).Conclusion Serum level of IL-22 increases in type Ⅰhypersensitivity disease patients,was is positive correlated with the number of sensitized allergens.

3' Untranslated Regions ; genetics ; 5' Untranslated Regions ; genetics ; Enterovirus B, Human ; genetics ; Genome, Viral ; genetics ; Humans ; Poly A ; genetics

Objective To investigate the correlation of HIF-1α and hypoxia in keloids fibroblasts, and to investigate the mechanism that hypoxia promotes abnormal scarring by HIF-1α pathway. Methods Keloid fibroblasts cultured in vitro were placed in an incubator with different O2 concentrations. After 24 h, the keloid fibroblasts were collected for further study. Western blotting was performed to detect the expression of HIF-1α in the keloid fibroblasts. Results Relative amounts of HIF-1α in keloid fibroblasts cultured under O2 concentrations at 20 %, 10 %, 5 % and 1 % were 0. 007 ±0. 006, 0. 133 ±0. 006, 0. 537±0. 015 and 0. 903±0. 021, respectively. It indicated that hypoxia could increase the expression of HIF-lα in keloid fibroblasts. Conclusions Hypoxia can induce the expression of HIF-1α in fibroblasts of keloids. Moreover, there still is a positive relation between hypoxia and the expression of HIF-1α. Therefore, a close relationship exists between abnormal scarring and HIF-1α pathway by hypoxia.

One hundred and twenty stroke patients were randomly divided into rehabilitation group (n =60) and control group (n =60).Patients were assessed with National Institute of Health Stroke Scale (NIHSS) ,Fugl-Meyer Assessment (FMA),Bathel Index (BI) and World Health Organization Quality of Life Assessment Instrument Brief Version(WHOQOL-BREF) before and 6 weeks after treatment by the same doctor.The scores of NIHSS,FMA,BI and WHOQOL-BREF were improved significantly in rehabilitation group after treatment; while those of control group were no improved.The scores after treatment of rehabilitation group were significantly higher than those of control group ( P ＜ 0.05 ).

Objective To investigate the knowledge level and needs in Xinjiang Uygur and Han patients with ulcerative colitis (UC), compare the differences between the two ethnic groups. Methods A total of 194 Uygur and Han UC patients were investigated with the general information questionnaire, the Crohn′s and Colitis Knowledge Score (CCKNOW) questionnaire, disease related knowledge needs questionnaire, to analyze the investigate results. Results CCKNOW score of Uygur and Han UC were (6.9±3.5) points and (9.2±3.1) points respectively, and Uygur was significantly lower than that of Han (Z=-2.831, P=0.005). Knowledge accuracy of dietary (30% and 63%) and drug (45% and 44%) were higher than general information (24% and 32%) and complications (12% and 30%) . Disease related knowledge needs score of Uygur and Han UC patients were (168.2±15.6) points and (155.4±17.2) points respectively, the score of Uygur patients was significantly higher than that of Han patients (t=4.429, P=0.001).The highest disease related knowledge needs score was (4.7±1.0) points of reproductive knowledge for Uygur UC patients, for Han patients was knowledge of daily life which was (4.8 ± 0.8) points. Conclusions The disease related knowledge level of Xinjiang Uygur and Han UC patients are relatively low, especially lack of general knowledge and diet knowledge, disease related knowledge of Uygur UC patients are lower than Han patients. The needs of the disease knowledge are very high for two ethnic groups, different one has different ethnic, gender, age, education level, living environment, hospitalization times, areas of disease related knowledge needs are also different. It is necessary to choose the targeted education content and suitable education way according to individual differences for nursing staff.

Objective To investigate the conrelation between neutrophils lymphocytes ratio (NLR) and disease activity of ulcerative colitis (UC).Methods Case-control study was used to compare NLR differences of 82 active UC patients,45 inactive UC patients,and 254 healthy controls.The multivariate analysis was applied to analyze the correlation between the NLR,erythrocyte sedimentation rate (ESR),Creactive protein (CRP),white blood cells (WBCs),and UC activity.The sensitivity and specificity of NLR to identify UC activity was evaluated.Results NLR of active UC group was 2.45 ± 1.22,which was significantly higher than that of inactive UC group and healthy control group,their NLR were 1.92 ± 0.68 and 1.83 ±0.75,respectively (H =9.991,P =0.007).The multivariate analysis showed that only CRP was correlated with UC activity (OR =1.396,95％ CI:1.086 ～ 1.795,P =0.009).Receiver operating characteristic (ROC) curve analysis showed that the optimum NLR cut-off point for UC activity was 2.23,the sensitivity and specificity were 55.82％ and 62.75％,respectively.Conclusions NLR has a certain reference value for the evaluation of UC activity,but it cannot be independent as a clinical index to evaluate the UC activity.

Objective To evaluate the value of monitoring circular blood interleukin(IL)-17 for predicting 60 d mortality rate in premature infants with necrotizing enterocolitis(NEC).Methods A total of 85 neonates with surgically or medically treated NEC were retrospectively included.Serum IL-17 level was detected in diagnosing NEC and before preparing operation for evaluating its predictive value.Results The mortality rate after 60 d was 20 ％ (17/85),in which 5 (10 ％)was in the medical treatment group and 12(34％) in the surgical treatment group.The IL-17 level was significantly correlated with mortality rate after 30 d [odds ratio (OR):1.38;CI:1.14-1.67;P=0.001].The preoperative IL-17 level in the surgical treatment group was 27-7 500 pg/mL(median 2 625 pg/mL),which was significantly higher than mean 156 pg/mL in the medical treatment group.The difference in the area under curve(AUC)between the medical treatment group and surgical treatment group was 0.82(CI:0.74-0.90).The critical value of IL-17 for detection significance was 1 783 pg/mL(sensitivity was 90.5％,specificity was 59.2％).Conclusion Serum IL-17 level is correlated with the mortality rate after 60 d.