Despite multimodel chemotherapy after successful surgical cytoreduction for ovarian cancer,disease recurrence continues to be problematic.Therefore,exploring new treatment strategy has already become urgent matter of the moment. Biological targeted therapies have been studied to overcome chemotherapy resistance.The most promising one at this time is bevacizumab.Results from phase Ⅱ and phase Ⅲ trials were excited.Deeper understanding of biologic pathway in ovarian cancer,and more biologic targets undergoing clinical trial will develop a new world for the treatment of ovarian cancer.

Dorsal-ventral axis formation is one of the earliest and the most important steps of patterning formation during early embryogenesis.The molecular basis of dorsoventral axis formation reflects the fundamental issues of orchestrated cell proliferation and differentiation.Wildly speculated since the Renaissance,the effort of deciphering the mechanisms of dorsal-ventral axis formation has contributed significantly to our current understanding of disease pathogenesis.Here,we focused our discussion on the recent discovery of the convergence of dorsal and ventral signaling pathways during early embryogenesis and its implications in cancer biology and beyond.

Objective_To identify the anti-HBV efficiency of AAV-IFNαby infecting HepG2.2.15 cell line, and to develop a new strategy of curing hepatitis B.Methods_Firstly,recombinant vector was packaged into AAV by three plasmids co-transfection method;then AAV-IFNαinfected HepG2.2.15 cell line, and samples were collected on the 1st, 3rd,6th and 9th day;thirdly, The time-course of HBV DNA,HBsAg and HBeAg were detected by qPCR and ELISA.The proliferation of HepG2.2.15 cells detected by MTT.Results_Recombinant AAV-IFNαcan significantly inhibit HBV replication and expression in vitro because of the proliferation of HepG2.2.15 cells inhibited by AAV6-IFNα( P<0.05) .Conclusions_AAV-IFNαis a potential antiviral product for hepatitis B.

Adenosine receptor agonists can activate adenosine receptors A 1 and A 3, thus trigger delayed preconditioning signal pathway and induce high expression of protective proteins and some ionic channel opening in myocardial cells, which reduces myocardial ischemia/reperfusion injury.

There were a lot of general researches and applications about constitution in Shanghan-Zongbing Lun ofPang Anshi. It is found that the constitution idea ofPang Anshi originated from Huangdi-Neijing. His constitution idea included that: the constitution formation was related to living environment and diet; the constitution factors should be considered in the clinical practice; the constitution factors should be considered when applicating Yang-preserving in spring and summer, Yin-preserving in autumn and winter; the constitution factors should be considered in the process of the choice of dosage, the method of decoction and the principle of medication. Pang Anshi constitution idea is of great important guidance for the later medical practice.

Enterostomy are a common surgical procedure in the treatment of rectal cancer, has more than 100000 each year in our country requires enterostomy patients, whereas patients with postoperative rehabilitation and nursing leave the family caregivers, the study found that with baseline levels of care for people, and increased mortality 63% of caregivers within five years, attaches great importance to the caregivers of patients with life can avoid the occurrence of another patient, at the same time also can improve the quality of family care, reduce the demand for medical and waste. Therefore, this article will from the permanent patients underwent colostomy care burden of family caregivers, health education needs, psychological burden to understand colostomy caregivers are faced with the problem of our country, explore the mental and physical health of the caregivers related factors, provide the basis for effective follow disease intervention.

The paper first sums up the types of clinical nursing ward rounds and points out that at present two types play a dominant role in China, viz. the problem-based nursing ward round and the nursing procedure-oriented nursing ward round. Then it expounds the goal of clinical nursing ward rounds, introduces the forms, participants, and schedules of nursing ward rounds, and analyzes the major problems that currently exist in this aspect. The paper argues that it is imperative for the hospital to reinforce the management of nursing ward rounds and ensure that they are well planned and prepared and highly standardized and focused so as to improve their quality.

BACKGROUND:Generaly, higher transcutaneous oxygen pressure indicates stronger aerobic fitness. Decreased transcutaneous oxygen pressure and increased transcutaneous carbon dioxide pressure reflect severe fatigue, muscle hypoxia, and poor function state.
OBJECTIVE:To observe changes in transcutaneous oxygen pressure and transcutaneous carbon dioxide pressure in biceps brachi of excelent male rowing athletes during 6-week altitude training, and analyze relationship of the changes to training load.
METHODS:The training containsthreestages,i.e., before, during and after altitude training. Transcutaneous oxygen pressure and transcutaneous carbon dioxide pressure were determined using PeriFlux System 5000 laser Doppler flowmetryin athletesintheresting-fasting state in the morning. Lactic acid level in athletes was determined using 6-grade load test 1 week before altitude training and 2 weeks after the end of altitude training.
RESULTS AND CONCLUSION:Transcutaneous oxygen pressure and theratio of transcutaneous oxygen pressure to transcutaneous carbon dioxide pressure showed the W-shape change, and transcutaneous carbon dioxide pressure showed the V-shape change. These results suggest that the arrangement of the altitude training program is suitable, namely“reserve physical fitness at the plain stage, add amount and enhance degrees of altitude training load at the early and late stages”,andit can provide a better training load structure model for the post-altitude training. Transcutaneous oxygen pressure and transcutaneous carbon dioxide pressure, in particular the ratio of them,canbeused to evaluate the degree of exercise load that athletes receive. At the same time, capilaries oxygen can be quantified by testing transcutaneous oxygen pressure.Transcutaneous oxygen pressureis recommendedas a noninvasive index to monitor changes in aerobic capacity of athletes.

AIM: To investigate the influence of tumor necrosis factor ?(TNF ?) or angiotensin Ⅱ(Ang Ⅱ) on tissue factor(TF) gene expression and the regulatory effect of upstream sequence on TNF ? or Ang Ⅱ-induced TF gene transcription in human vascular endothelial cells.METHODS: TF mRNA in endothelial cells was analyzed by in situ hybridization. By means of gene recombinant technique, we constructed two luciferase reporter genes containing different upstream sequence of TF gene. The two luciferase reporter genes, together with the intracontrol plasmid pSV-?-gal were co-transfected respectively into cultured human vascular endothelial cells. The relative luciferase activities were detected.RESULTS: TNF? or Ang Ⅱ could increase the expression of TF mRNA in vascular endothelial cells. TNF? and AngⅡ could significantly increase the luciferase expression in the p-244/+121bp Luc transfected endothelial cells, respectively. The luciferase expression in the p-111/+121bp Luc transfected endothelial cells decreased significantly compared with that in the p-244/+121 bp Luc transfected endothelial cells and there was no significant difference compared with control group. CONCLUSION: Upstream sequence p-244/-112bp of TF gene may play an important regulatory role in TNF ? or AngⅡ-induced TF gene expression in human vascular endothelial cells.

Objective To observe the clinical effect of Oxygen Atomization Inhalation combinated with Tra -ditional Chinese Medicine (TCM) in the treatment of capillary bronchitis .Methods 82 Children with capillary bronchitis were divided into Observation Group (42 cases) and contral group (40 cases).Cases in the Observation Group underwent TCM Agreement medicinal broth and oxygen atomization inhalation , while cases in the contral group underwent Ribavirin atomization .The symptoms of disappear time of signs , clinical effect and adverse circumstance were observed.Results The total efficiency of the observation group was 95.2%, 80% in the contral group.The antifebrile time, disappear time of cough and dyspnea , of the observation group were better than that in the compari -son group.The difference was statistical significant (p <0.05).There was no obvious adverse reactions during the treating periods.Conclusion The clinical effect of Oxygen Atomization Inhalation with Traditional Chinese Medicine (TCM) Agreement Medicinal Broth on capillary bronchitis is confirmed , and the effect of Relieving symptoms , signs quickly and shorten disease time is obviously better than Pure western medicine treatment .In addition, the adverse reactions caused by medicine is also very small ,which is worth promoting for clinical application .