Objective To investigate the clinical manifestations,laboratory features,methods of diagnosis and treatment and prognosis outcome of intrahepatic cholestasis caused by citrin deficiency (NICCD).Methods Four patients in two months ages diagnosed as NICCD were investigated from February 2014 to December 2014 in the Children's Hospital of Wuhan.The diagnosis and treatment and prognosis were analyzes.Results All patients were in hospital due to the skin with yellow dye admission.The direct bilirubin elevated anomalies associated with different levels of blood lipid,blood ammonia and lactate metabolism were characterized by liver function tests.The hot spot of NICCD SLC25A13 gene mutations in the regional common mutations IVS13 + 1G/A,1638ins23,IVS11 + 1G/A,851del4,S225X,1800ins1,R605X,IVS6 + 5G ＞ A were detected by genscaning and genotyping.Genotyping of SLC25A13 gene in 4 cases were 1638ins23 and 851del4 two heterozygous mutations,851del4 homozygous mutation,851del4 heterozygous mutation,and IVS6+ 5G＞A heterozygous mutation,respectively.So these patients were confirmed as NICCD.The patients were cured by antiinfective and gallbladder back yellow symptomatic treatments.The liver function,blood ammonia,lactic acid and blood lipid were exanimated at the time of admission,1 weeks and 2 weeks after admission,respectively.The results indicated that the treatment effect were good.The liver function were restored to normal after two weeks of outpatient.Conclusion The diagnosis of NICCD needs a comprehensive analysis of clinical,biochemical,metabolic genomics,imaging and pathology.SLC25A13 gene analysis is the reliable basis for the diagnosis of the disease.It is the key for NICCD to diagnose early.It is important to distinguish bile deposition caused by biliary atresia and other related diseases from NICCD.The diet early replacement is good for the prognosis.

Objective:To evaluate the values of the endoscopic argon plasma coagulation (APC) in the treatment of gastrointestinal polyps. Methods:The gastrointestinal polyps confirmed by endoscopy and pathology were treated with argon plasma coagulation generated by ERBE APC 300 made in German ERBE Company. Results:A total of 78 polyps (59 flat and thin sessile polyps and 19 pedunculated polyps) in 23 patients were all cured with APC. 59 flat, thin and sessile polyps were only treated with APC, whereas 19 pedunculated polyps were treated with snared polypectomy and APC ablation of residual tissue. Time of coagulation for every polyp was determined by its size and location. Polyps became flat and thin after(1～7)times of coagulation. As many as 10 polyps could be treated at one time, but for patients with more than 10 polyps, several times of treatment were needed. Conclusion:Treatment of gastrointestinal polyps by APC is an effective and safety method with low side effects. It can be the first choice of methods for the treatment of flat and thin sessile gastrointestinal polyps or polypoid projection.

Objective:To explore the effect of Nimodipine on synaptophysin in diabetic rat.Methods:38 Wistar rats were divided into the normal control group, diabetic model group, the Nimodipine treatment group randomly. Streptozotocin-diabetic rat model was established. The diabetic rats were treated with Nimodipine for 12 weeks. Then, the learning and memory abilities of rats were tested with the Morris water maze.The mRNA and protein level of synaptophysin in hippocampus were detected by RT-PCR and immunohistochemical method. Results:Compared with diabetic model group, the Nimodipine treatment group showed a significant decrease in the mean time of escape latencies(P

Portal vein tumor thrombus (PVTT) influence the prognosis of hepatocellular carcinoma.The development of PVTT is a multi-factor,multi-part process.According to anatomic features of the portal vein in the liver and tumor thrombus of HCC developing modes,a uniform tumor thrombus types system (typesⅠ -Ⅳ) is recommended.Multi-modal therapy based on surgery,interventional therapy and radiotherapy can improve the curative effect enormously.

Objective　To study the visual electrophyological changes in　patients with pregnancy-induced hypertension syndrome(PIH).Methods　The visual evoked potentials (VEP) and electroretinogram (ERG) of 28 patients with PIH in 56 eyes were recorded.Results　The patients with PIH had pathologic visual electrophysiologic abnormalities. The VEP N75 peak latency significantly increased(P<0.05), and the ERG b wave latency in patients with PIH showed remarkable difference(P<0.05).Conclusion　Visual electrophysiological examination may be valuable in early diagnosis of retinal disfunction in patients with PIH.

Circulating tumor cells (CTCs) play pivotal roles for monitoring the tumor metastasis and prognosis.The nanotechnology provides a favourable platform for CTCs detection,and enables CTCs to be more promising for practical application.Meanwhile,the nanoscale device by virtue of nanotechnology has broad application prospects in eliminating CTCs and offers a new direction in the field of anti-cancer.

Objective To study the biological types on the seven isolates of Trichomonas vaginalis from Beijing, Hebei-Tangshan, Hebei-Chengde and Jiangxi-Jiujiang in the mainland of China. Methods The samples were analyzed by PAGE, isoenzyme stain and cluster analysis. Results The isoenzyme systems used in the study included MDH, LDH, G-6-PD, PGI and PGM. No difference in the isoenzyme patterns of G-6-PD and PGI was found among the seven isolates. The MDH and LDH patterns of Beijing 1, Beijing 2, Jiujiang 3 strains were identical, while they were distinguishable from those of Chengde, Tangshan, Jiujiang 1, Jiujiang 2 isolates. The PGM pattern of Beijing 1 and Beijing 2 isolates were same but was different from that of the remainders. Gene tree was constructed according to the isoenzyme profiles. The results showed that there are differences in the patterns of the five isoenzymes between the isolates of Beijing 1, Beijing 2, Jiujiang 3 and other four isolates, and Jiujiang 3 was different from Beijing 1, Beijing 2 slightly. Conclusion It seems reasonable to assume that there are at least three different biological types of Trichomanas vaginalis in China.

Objective To investigate the incidence of the common respiratory viruses in children with respiratory tract infection and to compare the results among different ages and to analyze the relationship between wheezing of children and various viruses.Methods Nasopharyngeal secretions of 618 children with respiratory infection hospitalized were assayed for 7 respiratory viruses, including respiratory syncytial virus ,adenovirus, influenza A virus, influenza B virus, parainfluenza virus Ⅰ 、 Ⅱ、 Ⅲ.Results (1) A total of 166 cases, affected with viral etiology,were detected in 618 samples,and the total positive rate was 26.86％.The most common virus was RSV (17.96％), followed by PIV Ⅲ (5.99％), IFA (1.62％), ADV (0.81％), PIV Ⅰ (0.49％).Moreover, 1 case infected with RSV and PIVⅠ was included, with the positive rate 0.16％.(2)The positive rates were different in various ages, with 14d ～ ＜ 1-year 35.04％, 1 ～ ＜ 3-year 18.25％, 3 ～ ＜ 6-year 17.11％ and ≥ 6-year 9.26％.(3) There were differences of positive rate of viruses among different respiratory infectious diseases, and capillary bronchitis occupied the highest percentage(47.37％).(4)Among all the detected children, infants were more likely to whee in auscultation of lung, and RSV was the primary etiology.Conclusion RSV,which was the primary viral etiology in children with wheezing, was the most common virus leading to respiratory infection of children, followed by PIV Ⅲ.In the children with respiratory tract infection, the viral infection tends to occur in younger children.

Objective To compare and analyze the clinical effects of two surgical methods in the treatment of multilevel cervical myelopathy.Methods The data of 86 patients with multilevel cervical spondylosis myelopathy were retrospectively analyzed from June 2011 to June 2015.48 patients treated with anterior decompression and bone graft fusion were set as A group.38 patients underwent posterior single open -door laminoplasty were set as B group. The changes of nerve function indexes were compared between the two groups before operation,6 months after opera-tion and the last follow -up.Results 6 months after operation,the JOA score of the two groups was significantly higher than before the operation,and the difference was statistically significant(tA =-6.438,tB =-7.425,P <0.05).After 6 months and the last follow -up,there was no significant difference in the JOA score between the two groups(tafter 6 months =-0.892,tlast fol ow -up =0.160,P >0.05).At the last follow -up,the excellent and good rate of A group was 70.83%,that of B group was 71.05%,and there was no significant difference between the two groups (χ2 =0.00,P >0.05).Two groups of patients were successfully completed surgery,the average operation time of A group was (148.75 ±40.68)min,that of B group was (109.34 ±35.61)min,the difference between the two groups was statistically significant(t =4.711,P <0.05).The amount of bleeding in A group was (157.82 ±51.40)mL,that of B group was (235.76 ±61.55)mL,the difference was statistically significant(t =-6.399,P <0.05).Conclusion Anterior subtotal decompression and bone graft fusion and posterior single open door surgery in the treatment of multi segment cervical spondylosis myelopathy can obtain satisfactory neurological function.According to the patients'clini-cal symptoms and complications to develop a reasonable surgical plan is the key to treatment.

Urticaria, which is called as“yin zhen”in traditional Chinese medicine, is a kind of allergic skin disease with comings and goings of wind clumps patches. Its episodes have close relationship with external infection of six evil factors. This article collected, classified, and studied literature of TCM doctors in different dynasties about urticaria and external infection of six evil factors, and expounded pathogenesis of urticaria in the aspects of wind pathogen, dampness pathogen, fire evil and other pathogenic factors. In the treatment of urticaria, internal treatment is divided into dispelling wind method, eliminating dampness method, clearing heat method and other methods. External treatment is concluded form the aspects of washout method, medicated ironing method, and moxibustion method. This article classified and dig some valuable information, with a purpose to provide reference and evidence for prevention and treatment of urticaria.