Objective:To investigate the effect of different depth of anesthesia monitoring in elderly cancer patients in the early postoperative cognitive dysfunction (POCD).Methods: 124 cases were received general anesthesia laparoscopic resection of colorectal cancer in elderly patients, and randomly divided into the observation group and the control group, each with 62 cases. The mean artery pressure (MAP) and heart rate of two groups of patients at different depth of anesthesia in each period were compared with the previous induction of anesthesia (t0), the organ before intubation (t1) and after intubation (t2), before pneumoperitoneum (t3) and after pneumoperitoneum (t4), after surgery (t5) and extubation (t6). The corresponding indexes were also compared between the two groups of patients. Results:In the control group, heart rate of t2, and t4~t6 increased significantly faster. The differences were statistically significant compared with the observation group (t=4.132,t=4.345,t=4.253,t=5.326;P<0.05). MAP parameters in the control group were significantly higher than that in the observation group and the differences were statistically significant (t=5.433, t=4.985,t=5.032,t=5.163;P<0.05). POCD in the observation group was significantly lower than that in the control group and the differences were statistically significant (x2=5.323,P<0.05).Conclusion: In elderly patients with laparoscopic colorectal surgery radical NTS will remain at D2 level, which can effectively reduce the incidence of POCD patients and help patients maintain stable hemodynamics. It is worth of further promoting in clinical.

Objective:To study the inhibiting effect of VEGF antisense oligonucleotides carried by liposome on the growth of human bladder carcinoma in nude mice. Methods:The nude mice model of human bladder carcinoma was established and VEGF antisense oligonucleotides were carried by liposome to be transfected.The cell proliferation index,microvessel density (MVD) and the expression of VEGF were detected with immunohistochmistry streptavidin-biotin peroxidase(SP) method and the cell apoptosis index was determined with Tunnel technology. Results:The growth inhibitory rate in antisense experimental group and sense experimental group were 71.08% and 7.19% respectively. The differences in the cell proliferation index,apoptosis index,microvessel density(MVD) and the expression of VEGF between the antisense experimental group and the sense experimental group or the control group had significances. There was no statistical significance in the detection indexes between the sense experimental group and the control group. Conclusions:The VEGF antisense oligonucleotides can inhibit the neovascularization and the growth of bladder carcinoma in nude mice,which might be an important gene therapeutic strategy for the bladder carcinoma in future.

Objective:To investigate the effect of inhibition of vascular endothelial growth factor(VEGF) antisense oligodeoxynucleotides(ASODN) on human bladder carcinoma in nude mice mediated by ultrasound microbubble. Methods:The mice were randomly divided into 3 groups when transplantation tumor was established: For UM+ASODN group, mixture of microbubble and VEGF-ASODN was inject into every mice from caudal vein plus ultrasound tumor local irradiation. For UM group, microbubble was inject into very mice from caudal vein plus local irradiation with the same condition. For control group, physiological saline was inject into every mice from caudal vein.The treatment was performed every 3 days with total 5 times.The volume change of mouse subcutane tumors was observed.Evaluate the expression of VEGF and CD34 and Ki67 with immunohistochemistry dyeing and calculate the microvessel density (MVD) and cell proliferation index;TUNEL assay was used to analyse the apoptosis of tumor cells. Results:The growth speed of nude mice tumor in UM+ASODN group was obviously slower than control group; The expression of VEGF and The MVD in UM+ ASODN group was lower than control group(P

Small cell lung cancer with brain metastasis is the most common type in brain metastasis tumors with the poor prognosis. Researches show that prophylactic cranial irradiation is not only effective in the prevention and treatment of limited disease brain metastases of small cell lung cancer,but also effective for extensive disease. Moreover,certain medications such as mannitol,nimustine etc also play an important sup-porting role,but there are many other uncertain parts in terms of prevention and treatment remaining controver-sial and needing further research.

Objective To measure the nursing time that the geriatric patients need daily in a large general hospital in Anhui province,thus to understand the direct and indirect nursing items and time.Methods Timing and recording nursing operations to meet the conditions of the geriatric patients for 7 consecutive days by field recording method were done.Results The average time of each nursing procedures was calculated.Direct nursing time was 146.10 min,accounting for 84.70％ of the total nursing time,including primary care (42.81％) and respiratory/drain management proportion (15.86％); Indirect nursing time was 26.39 min,accounting for 15.30％ of the total nursing time.The actual number of nurse needed was 158,the suitable bed protection ratio was 1 ∶ 0.68.Conclusions Direct nursing time of geriatric patients needed was longer than that of indirect nursing time.To increase direct nursing hours and reduce the indirect nursing time can satisfy the need of elderly patients.In addition,the current nursing staffing cannot satisfy the clinical needs of the elderly,thus,nursing resources allocation should be reasonable arranged as soon as possible.

he proangiogenic effect.

OBJECTIVE:To explore the teaching methods for pharmacognosy course in order to improve the quality of teach-ing. METHODS:In view of the disadvantages of the traditional teaching method,the teaching methods in pharmacognosy courses were reformed and practiced in many ways. RESULTS:Special seminar was set up,the role of teacher and student exchange was applied,traditional culture and knowledge was penetrated in class teaching,extracurricular activities were carried out and field prac-tice was added. CONCLUSIONS:Through the reform practise of the teaching method in pharmacognostic courses in pharmacy ma-jor in our university,the initiative and enthusiasm of students' learning has been improved,the ability of professional knowledge has been expanded,innovation and practical ability have been actively cultivated and good teaching effected has achieved. The quali-ty of the teaching in pharmacognosy has been greatly improved.

Age Factors ; Environmental Exposure ; Humans ; Neoplasms ; epidemiology ; prevention & control ; Research

Biological Therapy ; Carcinoma, Neuroendocrine ; Thyroid Neoplasms ; therapy

Objective To investigate the relation of peripheral leukocyte total and its differential counts with urinary albumin(UAlb)in patients with type 2 diabetes.Methods The 564 subjects with type 2 diabetes were stratified according to UAER levels.The total and differential leukocyte counts of peripheral blood were measured.Demographic and potential metabolic confounding factors were analyzed with multiple correlation and logistic regressions to evaluate the effects of leukocyte counts on UAER.Results With the increase of UAER,the peripheral total leukocyte,neutrophil,monocyte,eosinophil and basophil counts increased.In contrast,the lymphocyte count decreased.The differences persisted even after adjusting the influence of age,sex,duration of diabetes and other known risk factors.Conclusions Our study shows that peripheral leukocyte counts,even within the normal range,are related with UAlb in subjects with type 2 diabetes in a dose-dependent manner.