Objective:To investigate the effect of different depth of anesthesia monitoring in elderly cancer patients in the early postoperative cognitive dysfunction (POCD).Methods: 124 cases were received general anesthesia laparoscopic resection of colorectal cancer in elderly patients, and randomly divided into the observation group and the control group, each with 62 cases. The mean artery pressure (MAP) and heart rate of two groups of patients at different depth of anesthesia in each period were compared with the previous induction of anesthesia (t0), the organ before intubation (t1) and after intubation (t2), before pneumoperitoneum (t3) and after pneumoperitoneum (t4), after surgery (t5) and extubation (t6). The corresponding indexes were also compared between the two groups of patients. Results:In the control group, heart rate of t2, and t4~t6 increased significantly faster. The differences were statistically significant compared with the observation group (t=4.132,t=4.345,t=4.253,t=5.326;P<0.05). MAP parameters in the control group were significantly higher than that in the observation group and the differences were statistically significant (t=5.433, t=4.985,t=5.032,t=5.163;P<0.05). POCD in the observation group was significantly lower than that in the control group and the differences were statistically significant (x2=5.323,P<0.05).Conclusion: In elderly patients with laparoscopic colorectal surgery radical NTS will remain at D2 level, which can effectively reduce the incidence of POCD patients and help patients maintain stable hemodynamics. It is worth of further promoting in clinical.

Objective:To study the inhibiting effect of VEGF antisense oligonucleotides carried by liposome on the growth of human bladder carcinoma in nude mice. Methods:The nude mice model of human bladder carcinoma was established and VEGF antisense oligonucleotides were carried by liposome to be transfected.The cell proliferation index,microvessel density (MVD) and the expression of VEGF were detected with immunohistochmistry streptavidin-biotin peroxidase(SP) method and the cell apoptosis index was determined with Tunnel technology. Results:The growth inhibitory rate in antisense experimental group and sense experimental group were 71.08% and 7.19% respectively. The differences in the cell proliferation index,apoptosis index,microvessel density(MVD) and the expression of VEGF between the antisense experimental group and the sense experimental group or the control group had significances. There was no statistical significance in the detection indexes between the sense experimental group and the control group. Conclusions:The VEGF antisense oligonucleotides can inhibit the neovascularization and the growth of bladder carcinoma in nude mice,which might be an important gene therapeutic strategy for the bladder carcinoma in future.

Objective:To investigate the effect of inhibition of vascular endothelial growth factor(VEGF) antisense oligodeoxynucleotides(ASODN) on human bladder carcinoma in nude mice mediated by ultrasound microbubble. Methods:The mice were randomly divided into 3 groups when transplantation tumor was established: For UM+ASODN group, mixture of microbubble and VEGF-ASODN was inject into every mice from caudal vein plus ultrasound tumor local irradiation. For UM group, microbubble was inject into very mice from caudal vein plus local irradiation with the same condition. For control group, physiological saline was inject into every mice from caudal vein.The treatment was performed every 3 days with total 5 times.The volume change of mouse subcutane tumors was observed.Evaluate the expression of VEGF and CD34 and Ki67 with immunohistochemistry dyeing and calculate the microvessel density (MVD) and cell proliferation index;TUNEL assay was used to analyse the apoptosis of tumor cells. Results:The growth speed of nude mice tumor in UM+ASODN group was obviously slower than control group; The expression of VEGF and The MVD in UM+ ASODN group was lower than control group(P

After-department examination palys an important role in clinical teaching.The level of clinical teaching is one of the most important markers to evaluate the whole quality of the hospital.To improve the ability of teaching and teaching concept,we summarized the problem of after-department examination and put forward a series of reformation methods.

Objective To observe the effect of IL 11 on graft versus host disease and graft versus leukemia after allogenic bone marrow transplantation and related mechanism in acute lymphoblastic leukemic (ALL) mice. Methods Twenty nude mice and 20 BABL/c mice were randomly divided into 2 groups separately, total 4 groups. Group A and C, nude mice and BABL/c mice control group respectively; Group B and D, nude mice and BABL/c mice experimental group. The mice in the experimental groups were subjected to the subcutaneous injection of IL 11 2 days before transplantation and 7 days after transplantation, while those in the control groups not to IL 11. The effects of IL 11 on CD4 + and CD8 + T cells before and after transplantation in ALL mice were evaluated by flow cytometry. The survival time of ALL mice after marrow transplantation was recorded. The GVHD pathological changes of liver, spleen and intestine in ALL mice after transplantation were observed. The level of tumor necrosis factor ? (TNF ?) was detected by ELISA. Results In group D, IL 11 could significantly decrease the number of CD4 + T cells and increase CD8 + T cells simultaneously. IL 11 could obviously prolong the survival time, delay and relieve GVHD, and decrease the level of TNF ? in ALL mice after transplantation. Conclusion IL 11 could alleviate GVHD and retain GVL effect after allogenic bone marrow transplantation.

The effects of tumor promoter TPA, MZR and PB on gap junction intercellular communication in NIH/3T3 cells were studied using the scrape-loading/dye transfer technique. All of these 3 agents were shown to cause a dose-dependent inhibition of the intercellular communication. The blockage of intercellular communication induced by TPA and MZR was inhibited in the presence of the protein kinase C inhibitor, staurosporine or palmitoyl carnitine, but not inhibited in the casexinduced by PB. The results suggest that the serape-loading/dye transfer technique may be used as a rapid screening assay to detect the tumor promoters and protein kinase C may be involved in the genesis of intercellular communication blockage induced by TPA ane MZR, but not by PB.

OBJECTIVE:To explore the teaching methods for pharmacognosy course in order to improve the quality of teach-ing. METHODS:In view of the disadvantages of the traditional teaching method,the teaching methods in pharmacognosy courses were reformed and practiced in many ways. RESULTS:Special seminar was set up,the role of teacher and student exchange was applied,traditional culture and knowledge was penetrated in class teaching,extracurricular activities were carried out and field prac-tice was added. CONCLUSIONS:Through the reform practise of the teaching method in pharmacognostic courses in pharmacy ma-jor in our university,the initiative and enthusiasm of students' learning has been improved,the ability of professional knowledge has been expanded,innovation and practical ability have been actively cultivated and good teaching effected has achieved. The quali-ty of the teaching in pharmacognosy has been greatly improved.

OBJECTIVE To evaluate the relationship between and antimicrobial usage antimicrobial susceptibility of Pseudomonas aeruginosa(PAE) causing nosocomial infections. METHODS Monitored the data on the yearly patient-days and the yearly consumption(defined daily dose(DDD) per 1000 patient days) the consumption of ceftazidime,cefepime,levofloxacin,piperacillin/tazobactam,cefoperazone/sulbactam,mipenem and meropenem from Jan 2003 to Dec 2007.was analyzed.Disk susceptibility data of P.aeruginosa(PAE) causing nosocomial infections were tested. RESULTS The yearly patient-days of our department significantly increased from 60 069 in 2003 to 74 442 in 2007(P

The ancient books on traditional Chinese medicine in Jiangsu Province were recorded and analyzed, fol-lowed by an introduction of their holdings in Jiangsu Province, and suggestions were put forward for their systemiza-tion, such as enforcing the role of special libraries of traditional Chinese medicine, promoting the general survey and report of ancient books on traditional Chinese medicine, and compiling《Jiangsu union catalogue of ancient books on traditional Chinese medicine》.

Objective To investigate the relation of peripheral leukocyte total and its differential counts with urinary albumin(UAlb)in patients with type 2 diabetes.Methods The 564 subjects with type 2 diabetes were stratified according to UAER levels.The total and differential leukocyte counts of peripheral blood were measured.Demographic and potential metabolic confounding factors were analyzed with multiple correlation and logistic regressions to evaluate the effects of leukocyte counts on UAER.Results With the increase of UAER,the peripheral total leukocyte,neutrophil,monocyte,eosinophil and basophil counts increased.In contrast,the lymphocyte count decreased.The differences persisted even after adjusting the influence of age,sex,duration of diabetes and other known risk factors.Conclusions Our study shows that peripheral leukocyte counts,even within the normal range,are related with UAlb in subjects with type 2 diabetes in a dose-dependent manner.