Laser photocoagulation,intravitreal injection of antibody against vascular endothelial growth factor (VEGF) or corticosteroids and pars plana vitrectomy are current popular therapeutic approaches for diabetic retinopathy (DR).However,some DR patients still progress to irreversible blindness even after the above treatments which do not aim at the pathological mechanisms and influence factors for DR.Thus,with the further elucidation on the molecular pathological mechanisms and overall understanding of the factors affecting DR development,more and more potential therapeutic interventions such as neuron protection,vascular reconstruction and protection,gene therapy,non-VEGF dependent antineovascularization agents have been explored.Individual precise therapy based on the potential therapeutic targets would provide the promising future for DR patients.

Sepsis is one of the serious illnesses leading to high mortality in ICU.Some animal experiments and clinical trails show that target temperature management has a protective effect on target organ function in sepsis,but the optimal timing,length of targeted temperature managment continued,intervention measures,and optimal target temperature obtained in sepsis remains in the great controversy.Whether the patients with sepsis would be truly benefited from the body temperature management is still worthy issue for further study.The aim of this article reviews is to trace the recent research progress on the body temperature management in sepsis.

The initial definition of stress and its development were briefly retrospected with elucidating the significance of stress in life sciences study. Stress is involved in a variety of physiological and pathophysiological processes. Description of the advances focusing on the relationships between stress and several body systems including nervous system, immune system and cardiovascular system etc. and on the stress molecules and signal transduction was carried out. The ultimate aim of the review is to emphasize the importance and the distinct position of stress during the development of modern bio-medicine, and to further attract more attention to the research field of stress from more scientists.

Objective To research the optimal preparation technology of salinomycin micelle.Methods DSPE-PEG2000 was selected as the carrier.Salinomycin was selected as the model drug.The film dispersion method, the ethanol injection method and the dialysis method were used to prepare salinomycin micelles respectively.The comprehensive evaluation indexes included entrapment rate and drug-loading rate, release capacity and vitro cytotoxicity test in order to select the most suitable preparation technology of salinomycin micelle .Results The film dispersion method is the most suitable preparation technology of salinomycin micelle in the three methods.Its average grain diameter was (14 ±2.3) nm, entrapment rate was (82 ± 2.6)%, drug-loading rate was (6.3 ±2.1)%, IC50 to HepG2 tumor cells was 16.10 ±3.71.Conclusion The film dispersion method of salinomycin micelles has the advantages with the smallest size, the highest entrapment rate and the largest drug-loading rate, which has the function to kill tunmor cells and release slowly.

Objective:To establish a detection method for residual toluene in diclofenac sodium raw material by GC. Methods:An HP-5 column (0. 53 mm × 30 m, 2. 65 μm) with nitrogen as the carrier gas was employed for the separation. The temperature of the column, injection port and FID detector was set at 50℃, 200℃ and 250℃, respectively. An internal standard method with isooctane as the internal standard was used to assay the residual amount after the sample was dissolved in DMSO. Results:The linear calibration curve was obtained over the range of 9. 27-111. 19μg·ml-1(r=0. 999 4). The mean recovery was 97. 27%(RSD=2. 80%,n=9). The LOD and LOQ was 0. 59μg·ml-1 and 1. 46μg·ml-1 , respectively. The residual contents of toluene in 3 batches of the products all met the relevant regulations. Conclusion:The established method is reliable and accurate, and can be used in the quality control of diclofenac sodium.

Surgery in combination with chemotherapy and radiotherapy is the standard of lung cancer treatment,but postoperative recurrence is very common which usually leads to higher mortality and lower life quality. Immunotherapy on post-operative patients fully mobilizes the body's defense mechanisms,activates the immune cells,and kills residual cancer cells. Current research on lung cancer immunotherapy mainly includes four categories:adoptive immunotherapy,dendritic cell vaccine,non-specific antigen immune therapy and anti-gen-specific vaccine. These studies show lung cancer immunotherapy intervention can effectively reduce postop-erative residual cancer cells,reduce postoperative recurrence rate,prolong survival,significantly improve the prognosis,and is worth spreading in clinical practice.

Objective To prepare the long‐term use and good stability of HBsAg feeble masculine serum ,and the application of controlling quality for HBsAg .Methods The HBsAg positive serum was diluted using calf serum phosphate buffer by grads ,multi‐ple to 1∶2 ,1∶4 ,1∶8 ,1∶16 ,1∶32;HBsAg feeble masculine serum was detected by double antibody sandwich method of enzyme‐linked immunosorbent assay (ELISA) using different reagent lot obtained from two companies for continuous six months .Results The serum ,s diluting multiple which the S/CO ,value between 1 to 3 was 1∶8 ,and the S/CO was 2 .42 ,the CV was 4 .72% .The serum of quality control possessed a good stability in different detection reagents ,the S/CO value range from 2 .41 to 2 .82 ,and the CV value was 4 .24% to 8 .68% .The CV value of HBsAg feeble masculine control serum with 6 months successive detection ranged from 8 .71% to 9 .86% (CV<10% ) .Conclusion The HBsAg feeble masculine serum possessed good stability and precision which is fit for masculine serum in HBsAg test .

Postoperative pulmonary artery stenosis is one of the popular complications.The disappointing results of surgical treatment for such lesions led to the introduction of interventional procedures (balloon angioplasty and stent implantation).Balloon angioplasty is applicable for branch pulmonary artery stenosis,however,balloon dilation alone rarely been effective in the long-term for these lesions.Since pulrmonary artery stenting was introduced in 1991,indications and use of stents for dealing with pulmonary artery stenosis have played a leading role in recent times.Intravascular stents are effective for stenotic artery dilation,can reduce right ventricle pressure and improve heart function.Excellent mid-and long-term results after stenting of postoperative pulmonary artery stenosis are demonstrated by numerous researches,with the new developments in stent design,indications for pulmonary artery stent implantation are likely to widen in the future.

Objective:To explore the relationship between carotid atherosclerosis (CAS)detected by ultrasonography and ECG abnormality and correlation between CAS and blood pressure in patients with coronary heart disease (CHD).Methods:A total of 389 CHD patients were divided into CAS group (n=213)and no CAS group (n=176).ECG abnormalities were compared between two groups,and ECG and blood pressure were compared among patients with different degrees of CAS in CAS group.Results:Compared with no CAS group,there were significant rise in constituent ratios of atrial fibrillation,Ⅱ~Ⅲ°atrioventricular block,total lead number of ST segment de-pression,and of abnormal T wave,ST segment deviation complicated abnormal T wave and angina pectoris compli-cated ST segment depression in CAS group (P <0.05~<0.01).Along with CAS grade increased (grade I~ grade IV),there were gradual rise in percentages of atrial fibrillation (8.33% vs.21.33% vs.31.03% vs.45.00%),to-tal lead number of ST segment depression (17.64% vs.27.22% vs.37.07% vs.52.08%),total lead number of ab-normal T wave (5.00% vs.6.56% vs.10.34% vs.22.08%)and ST segment deviation complicated abnormal T wave (26.67% vs.37.33% vs.50.00% vs.60.00%),P <0.05 or <0.01. There were no significant difference in diastolic blood pressure (DBP)between no CAS group and CAS group,but systolic blood pressure (SBP)and pulse pressure (PP)of CAS patients were significantly higher than those of no CAS patients,and they gradually elevated along with CAS severity increased (P <0.05~<0.01).Pearson correlation analysis indicated that CAS was signifi-cant positively correlated with SBP and PP (r =2.064,1.348,P <0.01 both).Conclusion:The incidence rate of abnormal ECG in CHD patients complicated CAS is higher than that of CHD patients without CAS;and CAS is pos-itively correlated with blood pressure.Therefore,carotid ultrasonography can better reflect CHD patients′condition.

Objective To investigate the bacterial spectrum changes and characteristics of drug resistance of patients with severe acute pancreatitis (SAP) and to guide clinical proper use of antibiotics medicine .Methods Clinical specimens of 50 patients with SAP were analyzed including sputum ,blood , urine ,central venous catheter ,bile ,et al .Bacterial strains were regularly isolated and drug sensitivity test was made by disc diffusion method .Chi-square test was performed for statistical analysis .Results One hundred and fifty-six bacterial strains were isolated .The number of strains isolated from the sputum , blood ,pancreas and abdominal cavity ,bile ,urine tract ,surgical incision ,oral secretion was 51 ,37 ,24 , 23 ,11 , 8 and 2 , respectively . The most common bacterial strains were Acinetobacter baumannii , Escherichia coli ,Pseudomonas aeruginosa and Enterococcus f aecium ,the number of strains was 30 ,21 , 20 and 14 ,respectively .The drug resistance rate of Acinetobacter baumannii to imipenem and meropenem was no less than 90% .The drug resistance rate to cefoperazone/sulbactam was lower ,but still over 60% . The drug resistance rate of Escherichia coli to penicillins ,majority of cephalosporins and quinolones was over 90% . The drug resistance rate of Pseudomonas aeruginosa to partial cephalosporins was high (90% ) ,and the drug resistance rate to imipenem was also up to 65% . T he drug resistance rate of Enterococcus faecium to penicillin G and quinolones was up to 100% . No Enterococcus resistant to tigecycline ,vancomycin and linezolid was found .The infection rate of patients received invasive operation was higher than that of patients received no invasive operation .Conclusions The main bacteria of patients with SAP complicated with infection was Gram-negative bacteria ,which has high drug resistance .The common locations of infection were respiratory tract ,blood ,abdominal cavity ,biliary system and urinary tract .The infection in respiratory tract and blood may be related with invasive medical operations .