Objective To investigate the distribution of traditional Chinese medical syndromes and clinical characteristics of influenza in South of Five Ridges.Methods A retrospective analysis was carried out in 162 cases of influenza patients admitted from outpatient department,emergency department and inpatient department of the First Affiliated Hospital of Guangzhou University of Chinese Medicine from 2014 to 2016.The distribution of clinical manifestations and syndrome types of the included influenza patients was analyzed.Results The average age of the included influenza patients was 35.76 ± 11.4 years old.The clinical syndromes were mainly characterized by fever,aversion to cold and chills,fatigue and weakness.And damp-accumulation manifestations of heaviness in the body,poor appetite,dry mouth without willing to drink,nausea and vomiting were also predominant.The main syndrome types were wind-heat attacking defense phase syndrome,wind-cold fettering exterior syndrome,heat-toxin attacking lung syndrome,heat-toxin accumulating lung syndrome,and damp syndrome.Of the syndrome types,wind-heat attacking defense phase syndrome and heat-toxin attacking lung syndrome were the leading types,accounting for 77.79％ and interweaving with damp syndrome and heat-damp syndrome.Conclusion The syndromes of influenza patients in South of Five Ridges are usually complicated by damp syndrome or damp-heat syndrome,and the predominant syndrome type is wind-heat interweaved with damp syndrome,which is correlated with the climate being damp and hot in South of Five Ridges.

Objective To determine the median effective dose (ED50 ) of ropivacaine for spinal anesthesia when combined with sufentanil in patients undergoing caesarean section. Methods Twenty-eight ASA Ⅰ or Ⅱ parturients, aged 18-40 yr, weighing 50-110 kg, undergoing cesarean section under combined spinal-epidural anesthesia, were enrolled in this study. Combined spinal-epidural anesthesia was performed at L2,3 interspace. The mixture of ropivacaine and 5 fig sufentanil was injected into the subarachnoid space over 30 s. The initial dose of ropivacaine was 11 mg. The dose was increased/decreased by 1 mg in the next patient. The ED50 and 95% confidence interval were calculated by up-and-down method. Results The ED50 of ropivacaine was 7.780 mg (95% confidence interval 6.850-8.836 mg). Conclusion When combined with sufentanil 5 μg, the ED50 of ropivacaine for spinal anesthesia is 7.780 mg in patients undergoing caesarean section.

Objective To evaluate the potential feasibility of γ-H2AX foci as a biodosimetry after exposure to ionizing radiation by comparing DNA double-strand break repair kinetics in rat blood lymphocytes with that in human lymphocytes.Methods Peripheral blood lymphocytes separated from Sprague-Dawley(SD) male rats and healthy adults were exposed to γ-rays,and some rats were also subjected to total body irradiation.The inductions of DNA repair-related foci of γ-H2AX,pATM (S1981) and pDNA-PKcs (T2609) were detected with immunofluorescence staining technique at different time points post-irradiation,and the status of their co-localization was analyzed.Results The induction kinetics of γ-H2AX foci in rat lymphocytes was similar to that observed in human lymphocytes.The frequencies of γ-H2AX foci peaked at 30 min after γ-ray irradiation (trst =62.64,th =28.52,P ＜ 0.05),then decreased rapidly after 6 h post-irradiation (trat =45.96,th =14.80,P ＜0.05),and the residual foci number remained only about 3％-8％ of its maximal value at 24 h post-irradiation.At 30 min after γ-ray irradiation,the frequencies of pATM (S1981) and pDNA-PKcs (T2609) foci in rat and human lymphocytes significantly higher than those of nonirradiated control (trat =21.05,25.80,th =11.07,29.52,P ＜ 0.05),and the frequencies of co-localization of pATM (S1981) or pDNA-PKcs (T2609) foci with γ-H2AX foci also markedly increased by 26％-32％ in irradiated lymphocytes of rat and human (trat =5.34,9.14,thuman =18.32,51.28,P ＜0.05).Moreover,γ-H2AX foci incidence in rat lymphocytes in vitro was consistent with that induced by total body irradiation of rat.The number of γ-H2AX foci in irradiated rat lymphocytes increased with irradiation dose in a linear dose-dependent manner,its slope was similar to that of irradiated human lymphocytes reported by other laboratory.Conclusions Rat is a useful animal model to evaluate radiation biodosimetry with γ-H2AX foci in lymphocytes.The co-activation of ATM and DNA-PK plays an important role in DSB repair in the irradiated lymphocytes of rat and human.

Chromatin accessibility is a highly informative structural feature for understanding gene transcription regulation,because it indicates the degree to which nuclear macromolecules such as proteins and RNAs can access chromosomal DNA.Studies have shown that chromatin accessibility is highly dynamic during stress response,stimulus response,and developmental transition.More-over,physical access to chromosomal DNA in eukaryotes is highly cell-specific.Therefore,current technologies such as DNase-seq,ATAC-seq,and FAIRE-seq reveal only a portion of the open chro-matin regions(OCRs)present in a given species.Thus,the genome-wide distribution of OCRs remains unknown.In this study,we developed a bioinformatics tool called CharPlant for the de novo prediction of OCRs in plant genomes.To develop this tool,we constructed a three-layer con-volutional neural network(CNN)and subsequently trained the CNN using DNase-seq and ATAC-seq datasets of four plant species.The model simultaneously learns the sequence motifs and regu-latory logics,which are jointly used to determine DNA accessibility.All of these steps are integrated into CharPlant,which can be run using a simple command line.The results of data analysis using CharPlant in this study demonstrate its prediction power and computational efficiency.To our knowledge,CharPlant is the first de novo prediction tool that can identify potential OCRs in the whole genome.The source code of CharPlant and supporting files are freely available from https://github.com/Yin-Shen/CharPlant.

Subclinical hypothyroidism is a common endocrine disease, and the clinical incidence rate has increased gradually in recent years. This disease has impacted on cardiovascular function, lipid metabolism, etc, which can leads diseases such as atherosclerosis, coronary heart disease. The screening and treatment for patients with subclinical hypothyroidism and the follow-up study have become a research hotspot in the field of endocrine at home and abroad in recent years. The consensus has not been reached about the outcome and prognosis of subclinical hypothyroidism. This article will conduct a literature review on the effects of subclinical hypothyroidism on the body, the intervention mode, the research progress and clinical follow-up to cause attention from endocrine professional medical staffs in patients with subclinical hypothyroidism, strengthening the management and follow-up of this group and improving the level of patients with subclinical hypothyroidism health and life quality, prolonging their life expectancy.

Objective To evaluate the effects of complications hazard experience on self-management behaviors, glycosylated hemoglobin and lipid among type 2 diabetes patients with poor glycemic control. Methods A total of 100 patients with type 2 diabetes were randomly divided into control group (50 cases) and intervention group (50 cases) by random number table. The patients of control group received routine diabetic education, while the patients of intervention group received the hazard experience of complications. All patients of two groups received telephone follow-up for 3 months. Their self-management behaviors, glycosylated hemoglobin and lipid were measured before and 3 months after the intervention. Results There was no statistically significant difference of the score in self-management behaviors, glycosylated hemoglobin and lipid between two groups before the intervention (P>0. 05). After 3 months intervention, the scores of balanced diet, regular exercise, blood glucose monitoring, foot care and the level of blood sugar handling in the intervention group were statistically higher than those in the control group (P <0. 05). The score of fully compliance medication had no statistically significant difference between two groups after the intervention (P>0. 05). The glycosylated hemoglobin, total cholesterol (TC), triglyceride (TG), low density lipoprotein in the intervention group were statistically lower than those in the control group (P<0. 05). Conclusions Hazard experience of complications can improve self-management behaviors, glycosylated hemoglobin and lipid for type 2 diabetes patients with poor glycemic control.

Objective To observe the influences of nursing intervention in diabetics,guided by protection motivation theory (PMT),of their protection motivation of blood sugar monitoring,frequency of monitoring blood sugar and glycosylated hemoglobin,and level of their blood sugar and glycosylated hemoglobin.Methods A total of 100 diabetics eligible for this research were randomly divided into intervention group and control group,each with 50 cases.The patients in the control group were given routine nursing and the patients in the intervention group were given a further 6-month protection motivation theory-based nursing intervention.Changes in protection motivation of blood sugar monitoring,in frequency of monitoring blood sugar and glycosylated hemoglobin,and in index of blood sugar and glycosylated hemoglobin were compared.Results Before the intervention,comparison between scores in each dimension of protection motivation in blood sugar monitoring,frequency of monitoring blood sugar and glycosylated hemoglobin,and values of blood sugar and glycosylated hemoglobin between the two groups were not statistically significant (P ＞ 0.05).After the 6-month intervention in the intervention group,the susceptibility score was (6.530 ±0.952);the self-efficacy score was (9.462 ±0.617);blood glucose monitoring frequency was (24.126 ±2.712) times;glycosylated hemoglobin monitoring frequency was (2.080 ±0.868) times;fast blood sugar was (7.080 ± 0.824) mmol/L;glycosylated hemoglobin was (6.823 ± 0.158) ％;the scores and values in the control group were (3.820 ± 0.723),(6.729 ± 1.341),(18.528 ± 2.831) times,(1.812 ± 0.778) times,(8.488 ± 1.592) mmol/L,and (9.853 ± 0.899) ％,with significant differences (t =16.742,8.435,14.626,12.489,-15.537,3.395;P ＜ 0.05).Conclusions Nursing interventions guided by protection motivation theory can improve diabetics' protection motivation and promote behavior of blood sugar monitoring and their metabolic index up to standard.

Objective To evaluate the effects of the training program based on the competency of new nurses in neurology department.Methods Competency concept was used to analyze the needs,insure the objective,establish the training plan,and organize training for new nurses in neurology department from August 2015 to March 2016. Training program with the combination of group psychological counselling,scenario simulation training and assessment were performed. Observation indexes were analyzed using the Six-Dimension Scale of Nursing Performance (6-D Scale).Results Group psychological counselling played a crucial role in the elimination of new nurses′ strangeness so as to help them to adapt to clinical competence. Statistically significant differences in new nurses′ ability of planning and evaluation,ability of interpersonal relationship and communication,ability of education and training were observed before and after training based on 6-D scale (P<0.05).Conclusions The training program based on the competency can improve the planning and evaluation ability,interpersonal relationship and communication ability,education and training ability in new nurses,and play a positive role in the growth of new nurses to qualified nurses.

Objective To evaluate the effects of mindfulness training on diabetes-related distress and quality of life in patients with type 2 diabetes mellitus, so as to provide evidence for nursing interventions. Methods A total of 90 inpatients with type 2 diabetes mellitus were randomly assigned to a control group and an observation group of 45 cases. The control group received routine health education, while the observation group received mindfulness training by the mindfulness training team composed of seven members including psychological counselors and nurse educators. Both groups were followed up for 6 months, and the scores of diabetes-related distress and quality of life were measured before and 6 months after the intervention. t-test was adopted to compare the difference between the two groups. Results After the intervention, the total score and scores of each dimension of diabetes-related distress in the observation group were (1.81±0.40), (1.99±0.45), (1.45±0.20), (2.01±0.27), and (1.51±0.21) respectively. Correspondingly, the scores of the control group were (2.07±0.24), (2.21±0.40), (1.61±0.19), (2.57±0.25) and (2.20±0.23), which were all higher than the observation group, with statistical differences (t=3.54, 2.32, 3.69, 9.68, 14.11;P<0.05). The scores of quality of life in disease dimension, physiological dimension, psychological dimension, social dimension and satisfaction dimension were (28.41±5.40), (21.05±4.16), (21.08±5.16), (19.04±3.30) and (22.84±3.79) in the observation group, which were all lower than those in the control group respectively [(36.08±5.20), (23.80±5.00), (24.11±5.22), (21.34±3.47), (24.64±3.50)], the differences were statistically significant (t=6.51, 2.69, 2.63, 3.06, 2.22;P< 0.05). Conclusions Mindfulness training can reduce the degree of diabetes-related distress and improve the quality of life in patients with type 2 diabetes mellitus.

Objective:To explore the intention of exclusive breastfeeding and its influencing factors within six months after delivery of pregnant women in the third trimester of pregnancy in Xi'an.Methods:From January to February 2019, convenience sampling was used to select 2 671 pregnant women in the third trimester of pregnancy who underwent regular obstetric examinations in Obstetrics Clinic of five hospitals in Xi'an for a cross-sectional survey. Pregnant women were surveyed by means of a field survey using a self-designed Exclusive Breastfeeding Intention of Pregnant Women in the Third Trimester Questionnaire. Multivariate Logistic regression was used to analyze the influencing factors of the intention of exclusive breastfeeding among pregnant women in the third trimester. A total of 2 671 questionnaires were distributed, 2 479 valid questionnaires were recovered, and the valid recovery rate was 92.8% (2 479/2 671) .Results:A total of 60.8% (1 507/2 479) of pregnant women planned to exclusively breastfeed within 6 months of delivery. Pregnant women in the third trimester of pregnancy whose husbands ( OR=2.950, 95% CI: 2.097-4.066) and family members ( OR=2.171, 95% CI: 1.571-3.000) supported exclusive breastfeeding had high intention to exclusively breastfeed, and those who participated in breastfeeding courses had a high intention to exclusively breastfeed ( OR=1.436, 95% CI: 1.199-1.719) . Conclusions:More than half of third-trimester women plan to exclusively breastfeed within six months postpartum. Husband and family support and enrolment in breastfeeding classes increase the intention of pregnant women to exclusively breastfeed. Prenatal breastfeeding courses should be carried out to raise the awareness of pregnant women and family members about exclusive breastfeeding, and focus on the support of husbands and family members for exclusive breastfeeding, so as to increase the exclusive breastfeeding rate within six months after delivery.