Objective:To investigate effects of dendritic cells(DCs) pulsed with autologous hepatoma cell lysates on the immune function of the postoperative patients with hepatocellular carcinoma(HCC).Methods:DCs isolated from peripheral blood mononuclear cell of HCC patients were cultured and proliferated in vitro by using rhGM-CSF and rhIL-4,and then were pulsed with autologous hepatoma cell lysates.30 postoperative patients with HCC were randomly divided into 2 groups.15 cases were treated with dendritic cell vaccine.15 cases received only chemotherapy and were established as control group.Immune function、clinical effects in two groups of patients were compared and observed.Results: The levels of DC3~+、DC4~+/CD8~+ and NK cell in the DC vaccine group significantly increased after vaccination,while those of the control group had no significant changes.The levels of IgG、IgM、IgA in the DC vaccine group increased after vaccination,but didn't reach statistical significance.The concentration of IL-10 in the DC vaccine group significantly decreased after vaccination(P

Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Fatty Liver ; drug therapy ; prevention & control ; Humans ; Phytotherapy

Fatty Liver ; diagnosis ; epidemiology ; Prognosis

Objective To research the risk of osteopo'rotic vertebral fractures with PKP after the PMMA injected location and dose of occurrence of vertebral refracture.Methods 78 cases of vertebral osteoporotic thoracolumbar vertebral compression fracture patients with PKP treatmentin were selected.According to position of postoperative PMMA in the vertebral body,they were divided into the side of the group of 35 patients and a median group of 43 patients.Compared two groups of patients before and after surgery,VAS,ODI score,the SI and Cobb angle and analyzed the PMMA leakage occur with the relationship of the amount of bone cement injected.Results There were significant differences in visual analogue pain scale(VAS),Oswestry disability index(ODI),sagittal index(SI) and Cobb angle of each group preoperatively versuspostoperatively (all P ＜ 0.05).No statistical difference was noted in the VAS,ODI,SI and Cobb angle between two groups preoperatively versus postoperatively(all P ＞ 0.05).PMMA injection volume between the the leakage group and the non-leakage patients had statistically significant difference (P ＜ 0.05).Conclusion PKP could obtain better clinical therapeutic effect in osteoporotic vertebral fractures treatment.Different PMMA positions had no significant influence on the therapeutic results.PMMA injected dose is a risk factor for bone cement leakage,which will increase the risk of vertebral fractures again.

Objective To study the effect of simvastatin combined with trimetazidine on cardiac function and high-sensitivity C-reactive protein(hs-CRP) in patients with chronic heart failure.Methods 160 patients with chronic heart failure were divided into control group and the observation group according to the random number table.The control group was treated with diuretics,inotropic,vasodilators and other conventional treatment,on the basis of this,the observation group received simvastatin combined with trimetazidine treatment.The cardiac function and hs-CRP of the patients were observed,and the treatment effects were evaluated and analyzed.Results The total effective rate of the observation group was 82.50％,which was significandy higher than 52.50％ of the control group (x2 =16.4103,P ＜ 0.001).Compared with before treatment,IL-6,TNF-alpha and hs-CRP levels of two groups after treatment were significantly decreased (t =12.234,13.342,5.463,8.533,7.896,3.536,all P ＜ 0.05),and those in the observation group were significantly lower than the control group (t =4.653,3.976,3.674,all P ＜ 0.05).Conclusion Simvastatin combined with trimetazidine has good effect on the cardiac function of patients with chronic heart failure,and can effectively reduce the level of hs-CRP,it is worthy of clinical application.

Objective To explore the before taking finasteride on transurethral plasmakinetic resection of prostate(TUPKVP)patients about IPSS,QOL,Qmax,PVR and other indicators.Methods 180 cases with benign prostatic hyperplasia were randomly divided into observation group and control group,90 cases in each group.Observation group and control group were given conventional therapy and the observed patients took 5 mg of finasteride everyday one week before surgery,all patients were followed up for 1 month.The two groups of TUPKVP patients were compared about IPSS,QOL,Qmax and PVR,intraoperative blood loss,operation time,irrigation fluid volume indicators.Results 180 patients were given successfully TUPKVP surgery.IPSS,QOL,Qmax and PVR contrast were significantly improved after treatment(t=5.11,7.01,3.06,6.17,11.82,4.13,4.52,5.17,all P＜0.05).IPSS,Qmax,PVR of the observation group after treatment was significantly better than that of the control group(t=11.35,5.26,3.12,all P＜0.05),the QOL difference between the two groups after treatment was not statistically significant(P＞0.05).The cure rate of the observation group was 87.8％,which was significantly higher than 63.33％ in the control group(x2=14.56,P＜0.05).Intraoperative flushing fluid volume,intraoperative blood loss,duration of surgery in the observation group were significantly different compared with the control group,(t=21.15、43.12,5.33,all P＜0.05).Conclusion The preoperative taking finasteride can reduce TUPKVP blood loss and flushing fluid volume,shorten operation time and effectively improve the security of the operation.

Bone marrow stromal cells(MSCs) have been shown to be extremely versatile.Recently,it is demonstrated that MSCs differentiate into neurons.Moreover,under the appropriate conditions,MSCs differentiate into special phynotype of neurons,such as 5-hydroxytryptamine-sensitive neurons,and dopaminergic neurons.The mechanism remains to be elucidated,and an overexpression of neuronal specific genes(such as Sox,Pax,Notch gene) may play an important role,especially RB/PBO gene.However,RB triggers cholinergic differentiation.Extrinsic factor such as concentration of cAMP plays certain role in differentiation.MSCs have also been shown to have certain therapeutic effect on neuronal degeneration disease.These cells have great potential because it is easy to isolate,culture,amplify,and no ethics neither immunologic rejection problem was concerned.

The new,rapid,accurate and convenient tests for tuberculosis diagnosis and drug resistance are urgently needed for effective tuberculosis control.In this review,two novel,inexpensive diagnosis methods of active TB were introduced.They can detect tuberculosis disease and drug resistances directly form sputum specimens and might have greater applicability in developing countries.Several concerns about the research and development of innovative diagnosis technology in our country also are described.

We reviewed the role of COX-2 independent targets of celecoxib and related compounds in cancer prevention and treatment. Direct non-COX-2 targets of celecoxib might be protein-dependent kinase 1 (PDK-1),Ca+ ATPase and carbonic anhydrase (CA), and so on; all of which are inhibited by celecoxib and are the main components in various pathways. Inhibition of these proteins leads to the induction of apoptnsis, the inhibition of cell cycle progression, angiogenesis, and metastasis, which are the main mechanisms by which celecoxib exerts its anticarcinogenic activity.

Nrf2/Keap1/ARE is an important signaling pathway in maintaining the balance between peroxide and antioxidants. Once oxidative stress happened, Nrf2/Keap1/ARE would be ac-tivated to regulate the expression of downstream antioxidant pro-teins that could reduce the injury from oxidative stress and the level of oxidative stress. Recent researches show that Nrf2/Keap1/ARE signaling pathway is closely related to refractory re-spiratory diseases such as pulmonary fibrosis (PF), lung canc-er, chronic obstructive pulmonary disease (COPD), suggesting that Nrf2/Keap1/ARE signaling pathway may be a potential ther-apeutic target. This review focuses on the role of Nrf2/Keap1/ARE signaling pathway in refractory respiratory diseases in order to further understand the related mechanism and provide evidence for the diagnosis and treatment of refractory respiratory diseases.