OBJECTIVETo observe the dynamic changes of membrane-bound proton-translocating ATPase (F-ATPase) in the development of dental caries, the expression of Streptococcus mutans F-ATPase under different pH concentrations and during the development of dental caries is analyzed.

METHODSStreptococcus mutans cultured under different pH (pH4.0-7.0) concentrations and containing 5% glucose and no glucose containing BHI were collected. RNA was extracted. Subsequently, F-ATPase gene was detected using real-time polymerase chain reaction. Male Wistar rats were divided randomly into caries group and control group. The rats in the caries group were fed caries feed and 5% glucose water, whereas those of control group were fed normal feed. Total RNA was extracted from plaque samples, which were collected from rats' oral cavity every two weeks. F-ATPase gene was detected by real-time PCR. In the 11th week, the upper and lower jaw bone specimens of rats were taken, and molar caries damage assessed.

RESULTSThe expression of F-ATPase in the caries group was higher than that in the control group (P<0.05). In addition, the gene was expressed highest in pH5.0 and the lowest in pH4.0 (P<0.05). 2) The expression of F-ATPase progressively increased during the caries development in both groups; expression in the caries group was higher than that in control group (P<0.05).

CONCLUSIONAcid-resisting viru-lence factor F-ATPase is related closely with the incidence and development of dental caries.

Adenosine Triphosphatases ; metabolism ; Animals ; Dental Caries ; metabolism ; microbiology ; Dental Plaque ; microbiology ; Male ; Protons ; Random Allocation ; Rats ; Rats, Wistar ; Real-Time Polymerase Chain Reaction ; Streptococcus mutans ; drug effects ; genetics ; Virulence Factors

Objective In Caucasian populations, the tumor cells of Epstein Barr virus (EBV)-positive classical Hodgkin lymphoma (cHL) patients more frequently express HLA class Ⅰ and HLA class Ⅱ molecules compared to EBV-negative cHL patients. HLA expression (in relation to EBV) in Asian cHL patients has not been previously investigated. Methods 145 cHL patients with formalin-fixed, paraffin embedded tissue blocks available from Beijing, China were randomly selected. Hematoxylin & Eosin-stained slides were used to reclassify the histological subtypes according to the WHO classification. EBV status was determined by visualization of EBER in tumor cells using in situ hybridization. Membranous expression of HLA molecules was detected by immunohistochemistry using antibodies HC-10 (class Ⅰ heavy chain) and antiβ2-microglobulin for HLA class Ⅰ, and CR3/43 for HLA class Ⅱ. Results EBV (+) tumor cells were observed in 40 % (58/145) of the cHL patients. As expected, the percentage of EBV(+) cases was much higher in the mixed cellularity subtype (71%) than that in the nodular sclerosis subtype (16 %) (P ＜0.001). The expression of HLA class Ⅰ was observed in 79 % of the EBV (+) cHL cases and in 30 % of the EBV (-) cases (P ＜0.001). For HLA class Ⅱ, 52 % of EBV(+) cHL cases were positive, compared to 43 % in EBV(-) cases (P =0.277). Conclusion The results in China population were similar to that in the Caucasian population for HLA class Ⅰ, but not for HLA class Ⅱ.

As an important landmark, the posterolateral wall of maxillary sinus can help to locate numbers of significant signs such as maxillary artery and its branches, maxillary nerve and infraorbital nerve, infratemporal fossa and pterygopalatine fossa etc. in the endoscopic surgery for paranasal sinuses and lateral skull base. This article reviewed related researches about the anatomy and endoscopic surgery of posterolateral wall of maxillary sinus.
Endoscopy ; Humans ; Maxillary Artery ; Maxillary Nerve ; Maxillary Sinus ; anatomy & histology ; Nasal Surgical Procedures ; Pterygopalatine Fossa

This 9-year-old boy presented with dysphonia and dyspnea for one month. Laryngoscopy showed a tumor of anterior vocal cord. The patient received a tumorectomy, immunohistochemistry concluded to the diagnosis of inflammatory myofibroblastic tumor.
Child ; Humans ; Laryngeal Neoplasms ; Male ; Myofibroma

LL-37 is the only member of the cathelicidin family known to exist in human acting as an endogenous antibiotic.After removal of the highly conserved N-termini of precursor hCAP18,a 15 000 C-terminal fragment called LL-37 bearing all of the known biological acivity hitherto is liberated by proteinase.LL-37 is active against both Gram-positive and Gram-negtive bacteria.Its antibacterial activity depends on the formation of ?-helical conformation.The cytotoxic activity of LL-37 is inhibited by apolipoprotein A-Ⅰ(apoA-Ⅰ) in the plasma,thereby it can function as a scavenger of LL-37 and prevent host cell damage.In addition to its microbicidal activity,LL-37 has several other functions such as chemotactic,angiogenic action and the ability to bind and neutralize the endotoxin.

Objective To compare the efficacy and safety of duloxetine and gabapentin in the treatment of diabetic peripheral neuritis .Methods 135 patients with diabetic peripheral neuritis were divided into the observation group(71 cases) and the control group (64 cases) according to random number table method ,and all patients received hypoglycemic therapy based on diabetes treatment principle .Furthermore,the observation group received oral duloxe-tine,while the control group was given oral gabapentin .The pain scores of all patients were evaluated using visual ana-log scale(VAS) and medical outcomes study (SF-36),and the adverse reactions were recorded during treatment . Results (1) Compared with before treatment ,both VAS scores and SF-36 scores in observation group and control group declined significantly at the end of 1st,2nd,4th week after treatment(all P<0.05),while there were no statis-tically significant differences in VAS scores and SF-36 scores between the two groups at the same time point ( all P>0.05).(2)The incidence rates of dry mouth ,drowsiness,fatigue and dizziness in observation group were significantly lower than those in control group (18.31% vs.34.38%,4.23% vs.26.56%,0.00% vs.23.44%,0.00% vs. 15.63%,χ2 =4.059,13.308,4.059,4.059,all P<0.05),while there were no statistically significant differences in incidence rates of nausea ,diarrhea and flustered between the two groups (all P<0.05).Conclusion The duloxetine and gabapentin in the treatment of diabetic peripheral neuritis have similar efficacy , but the duloxetine has better security.

As the completion of human genome project, application of the knowledge and techniques developed during thpast decade to the medical practice become more and more important and gain more and more attention. It is worthwhile to look back to the step prints of the development of medical genetics in China.1 The sprout of genetics in ChinaIt should be mentioned fromthe very beginning of the birth of the genetics in China to understand the historymedical genetics in China. In 1922, C.CHEN (Zhen CHEN) opened genetic courses in National Southeast Unversity after he returned from the T.H.MORGAN’s lab at Columbia University. He used Goldfish as a model fogenetic study. The Chinese scholars started their work on human genetics by survey the frequency of ABO bloogroup in the Chinese. Their result was published in1918. This was the first data of gene frequency of Chinese population, followed by reports on the inherited disease in Chinese on the frequency of color blindness in 1937. I1948, T. C. HSU described the ability to fold up the tip of the tongue as a recessive trait (MIM189300)[1,2]C.C.LI (Ching Chun LI) wrote a book,Introduction to Population Genetics, which became a wellknown book ithe world and from which a whole generation of geneticists benefited.

Objective To investigate effects of sinusitis mixture on functional endoscopic sinus after surgery .Methods According random number table,36 cases of chronic nasosinusitis patients were divided into the treatment group and control group,the two groups were routinely 2%NaCl saline nasal irrigation,use of nasal corticosteroids and mucous discharging agent mixture used in postoperative patients with sinusitis in the treatment group oral therapy. Results The treatment group 100%,total efficiency in the control group (89%) (χ2 =4.36,P<0.05),there was a statistically significant difference.Conclusion Sinusitis mixture has significant efficacy on the recovery of patients afterfunctional endoscopic sinus surgery.

This article consults the literature on acupuncture-moxibustion treatment for the non-spastic stage of cerebral stroke published from 2001 to 2014, makes a summary and analysis from meridians, point selection, acupuncture reinforcing-reducing techniques and electoacupuncture application and points out some problems such as valuing acupuncture and neglecting moxibustion, valuing acupuncture and neglecting medicine, and neglecting point selection based on syndrome differentiation in acupuncture-moxibustion treatment for the non-spastic stage of cerebral stroke. The article also objectively assesses the position of acupuncture-moxibustion treatment, puts forward relevant solving ideas and emphasizes the importance of acupuncture manipulation and the necessity for combining Chinese herbal medicine to treat the non-spastic stage of cerebral stroke.

Hirschsprung's disease (HD) is a conginental disorder of enteric nervous system (ENS) and is associated with the abnormal migration of gut neural cerst cell (GNCC).Recent studies showed that the regulation of this migration is a complex gene network.By the development of epigenetic and stem cells,this network is becoming much more clear.Transplantation of the modified gut neural crest stem cells/gut neural crest precursors(GNCSC/GNCP) acquired from the HD bowl is becoming the potential therapy of HD.This paper summerizes the recent advances and is to promote better understanding the pathogenesis of HD.