1.The study of allergen of mare’s milk, airag and mugwort pollen
Oyuntsatsral B ; Gantulga B ; Munkhbayarlakh S ; Narantsetseg L
Mongolian Medical Sciences 2015;173(3):19-22
BACKGROUND: Mugwort is the important source of fall allergic symptoms in the Mongolia. Mugwort pollen allergicpatients frequently present allergic symptoms of ingestion after several kinds of foods.OBJECTIVE: We sought to study the clinical manifestations of airag (fermented mare’s milk) and mare’s milkhypersensitivity in patients with mugwort allergy, identify the molecular weight of allergens, andevaluate their IgE cross-reactivity.MATERIAL AND METHODS: We collected to mugwort pollens from around UB in august and practiced fresh airag and mare’s milk.Airag, mare’s milk and mugwort extracts prepared by Hames Richmond method and their allergenswere identified by means of SDS-PAGE. ELISA inhibition experiments were done to study crossreactivitybetween airag and mugwort.RESULTS: In SDS-PAGE determined mugwort allergen 12-43 kDa, mare’s milk allergen 13-70 kDa, airag allergen12-68 kDamolecular weight.The study of the cross-reactivity between mugwort allergens and some food allergens was becamepractical significance on diagnosis, treatment and prevention of respiratory allergies. We were definedallergenic cross-reactivity between airag allergens and mugwort allergens which are common causesof upper respiratory allergy. On the Mugwort-ELISA inhibition test, the 50 % inhibitory dose to MugwortspecificIgE was 0.01μg/ml Mugwort allergens and 0.025μg/ml Airag allergens.However, the 1.0 μg/mlof Mugwort and Airag allergens were completely inhibited to Mugwort-specific IgE and Airag-specificIgE antibodies.CONCLUSION: In determined the mugwort pollen has 5 band(12, 23, 28, 38, 43kDa), in mare’s milk (13, 15, 60, 70кДа) and airag(12, 30, 50, 68 кДа) has separately 4 bands allergen protein by SDS-PAGE. ELISAinhibition study was strong cross-reactivity between airag allergen and mugwort allergen.
2. DETERMINATION OF THE RISK FACTORS AND TREATMENT OF ECTOPIC PREGNANCY
Ankhgerel J ; Bolortuya B ; Gantulga N ; Enkhtuya M
Innovation 2015;9(3):41-44
Ectopic pregnancy (EP) is a life – threatening emergency, and a significant cause of maternal morbidity and mortality in Ulan – Bator. The aim of this research was to determine and evaluate the incidence, risk factors and treatment of ectopic pregnancies at The First Maternity Hospital in Ulan – Bator.This was a retrospective, descriptive study of EP’s managed in The First Maternity Hospital during the study period 2013, 2014.The medical records of the patients managed for ectopic pregnancy as well as the total birth record and gynecological admission records during the period under review were retrieved, and data were collected with the aid of data-entry forms designed for this purpose. There were 46.147 gynecological admissions and 28.744 deliveries, with 870 cases of ectopic pregnancies. A total 863 cases were suitable for analysis. The relevant collected data were analyzed with SPSS Version 20.0 for windows.Ectopic pregnancies constituted in 3.9% of all gynecological admissions.Majority of patients were in 25-29years age group (64.1%). The average age of patients was 30 +/- 6 years. In 83.8% of patients had previous abortions, inflammatory disease was the most frequent risk factors.521(60.3%) patients were treated surgically while the 342(39.6%) remain patients received conservative treatment.The incidence of ectopic pregnancy increased by 0.8%, which is comparable between 2013(2.5%), 2014(3.3%). In our study the number percentage of organ preserving treatment, such as tubostomy and conservative – treatment by methotrexate increased significantly.
3. RESULT OF PREMALIGNANT LESION OF CERVIX BY COLPOSCOPY AND DETERMINATION OF SOME RISK FACTORS
Batnyam B ; Unurjargal D ; Ariunbuyan D ; Gantulga D ; Bolorjargal E ; Bolormaa N
Innovation 2015;9(3):68-69
Cervical cancer is commonly caused by infection with human papilloma virus(HPV) and some risk factors are involved in the etiology of it.1 All over the world 437000 people are diagnosed with cervical disorders and half of them die due to cervical cancer.2 Annually 12000 new cases of cervical cancer are detected and 5000 women die because of it. In Spain about 2000 women are determined in the 3rd and 4th stage of the disease per year.3 Over the period 2000-2008 cervical cancer rate is 8 %among all cancers in Mongolia. Approximately 16 % of women’s cancer is cervical cancer. 4 In developing nations prevalence rate of cervical cancer is higher because of malnutrition, quality and framework for early detection are not satisfying and some reproductive risk factors also influence on it. 5 Worldwide diagnosing early and rapid management of precancerous condition and cervical abnormalities turn into main issue. Therefore based on these detection of premalignant lesion of cervix by colposcopy the main objective of the study. The overall goal of the study is to detect the premalignant lesion of cervix by colposcopy and determine of some risk factors and study the results.A total of 71 women, who are treated in Women’s inflammatory disease unit, Infertility and Women’s endocrine disorder unit are recruited for the cross sectional study. The women, who conducted the study were selected by accidently and colposcopy was done. They also have completed special questionnaires. The data were analyzed using the SPSS 19.0, Windows Office. The average age of the women was 38±9.4. Colposcopy was done 90.1% (n=64) of women, 9.9% (n=7) of women had not colposcopy. Among the women who had colposcopy, biopsies were taken 56.3% (n=36). During colposcopy we analyzed condition of cervix then we took biopsy from suspected areas and sent it histology laboratory. We compared predictive diagnosis, histology results after colposcopy and 33.3% (n=12) were identified as normal, CIN I was 52.7%, (n=19), CIN II was 5.5% (n=2), CINIII was 2.7% (n=1), cervical cancer is confirmed in 5.5% (n=2). We studied risk factors that can influence the cervical disorders among the women recruited in the study and age of first sexual intercourse (r=0.356, p=0.033), number of abortion (r=0.412, p=0.029) were statistically significant. However age of the women, parity, usage of contraceptive pills, smoking, number of sexual partners were statistically not significant.(p>0.05) When women’s age of first sexual intercourse is younger, cervical cancer disorder occurs30% greater comparing to women having first sexual intercourse later, (p<0.05, R=0.3), when number of abortion increases cervical cancer disorder increases 40%(p<0.05, R=0.41).F-1 to recruit osteoprogenitor /mesenchymal stem cells in the bone regeneration process.
4. SOME RESULTS FROM SERUM DEHYDROEPIANDROSTERONE SULFATE HORMONE DETECTION IN REPRODUCTIVE-AGED WOMEN WITH PCOS AND HEALTHY
Erdenetsetseg N ; Battamir U ; Enebish D ; Unurjargal D ; Gantulga D ; Bolorchimeg B
Innovation 2015;9(3):24-27
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder of reproductive-age women, affecting an estimated 5-8% of all women in this age group. Clinically, hyperandrogenism, chronic amenorrhea, central obesity and polycystic ovary can all occur in women with PCOS and may cause of infertility. Dehydroepiandrosterone sulfate is an androgenic hormone produced inadrenal cortex and recently, there is no any data determined DHEA-S in patients with PCOS.Our study was designed to evaluate obesity, central obesity, hirsute grade and infertility and to determine serum DHEA-S hormone in women with PCOS and healthy non-PCOS women.We have used a cross-sectional study design and the study included reproductive-age 36 women, of whom 18 were diagnosed with PCOS and 18 were non-PCOS. Anthropometric components were measured and ELISA test to determine serum DHEA-S hormone was analyzed for all of these women. When we analyzed ELISA test to determine serum DHEA-S hormone, DHEA-S level was2.9±2.0 µg/ ml in non-PCOS reproductive-age women and 8.0±2.1 µg/ml in women with PCOS, respectively (р=0.01). Among the women diagnosed with PCOS had higher incidence of central obesity and grade II hirsute and infertility than healthy women (p=0.001). By the result, higher level of serum DHEA-S in women diagnosed with PCOS may cause of sign of hyperandrogenism such as central obesity, infertility and hirsute.