Methods for the extraction and purification of poly-unsaturated fatty acid(PUFA)from viscerai oil of three fresh-water fish (bream,grass carp and silver carp)were studied- It was suggested that the medicinal quality standard of PUFA could be laid down by referring to the quality standard of Acidum Morrhuicum.

In Order to screen antidiarrhea drugs, the standard operation of mice diarrhea model replicated with leave of Cassia angustifolia vahl was introduced. In experiments, diarrhea index expressed with loose stool ineidence rate multiplied by loose stool grade was used as main index. It thoroughly mirrors indi. vidual diarrhea degree and possesses comparability. The result indicates that diarrhea index in mice presented normal distribution. The index between animal model groups kad no difference as well as no differcnce was found in model mice of same group for six consecutive days, with good reproduclbility. It is recommended that the model can be used for the screening of antidiarrhea drugs.

Objective: To investigate the effects of berberine combined with simvastatin on the expressions of vascular endothelial growth factor (VEGF) ∗ transforming growth factor-(TGF-β1)∗ interleukin-18 (IL-18) and interleukin-10 (IL-10) in the thoracic aorta tissue of the atherosclerosis (AS) model rats∗ and to elucidate the mechanism of anti-atherosclerosis of berberine combined with simvastatin. Methods: A total of 48 rats were divided into control group, model group, simvastatin group, low. middle and high doses of berberines combined with simvastatin groups ( n = 8). The serum and thoracic aorta tissue of the rats were obtained 4 weeks after adminstration. HE staining was used to observe the pathomorphology of the thoracic aorta of the rats in various goups; ARC-double antibody sandwich EL ISA method was used to detect the serum VEGF, TGF-j31. IL-18 and IL-10 of the rats in various groups; automatic biochemistry analyzer was performed to determine the levels of senim total cholesterol (TC) • triglyceride (TG). low density lipoprotein cholesterin (LDL-C)∗ high density lipoprotein cholesterin (HDL-C) of the rats in various groups. The expression levels of VEGF. TGF-,β1. IL-18. and IL-10 proteins in the aortic tissue of the rats in various groups were determined by immunohistochemistry. Results: The HE results showed that compared with control group, the intima of aorta of the rats in model group was significantly thickened, lipid deposition was observed inside and outside the cells, necrosis was observed under the intima. plaques and irregularities were observed in the inner wall, and the vascular cavity was narrowed; compared with model group, in high dose of berberine combined with simvastatin group, there was no obvious plaque on the arterial wall, and the intimal thickening was significantly improved. Compared with model group, the serum TC, TG, and LDL-C levels of the rats in simvastatin group and different doses of berberine combined with simvastatin group were significantly decreased ( P

OBJECTIVE: To present a nonlocal low-rank and sparse matrix decomposition (NLSMD) method for low-dose cerebral perfusion CT image restoration. METHODS: Low-dose cerebral perfusion CT images were first partitioned into a matrix, and the low- rank and sparse matrix decomposition model was constructed to obtain high-quality low-dose cerebral perfusion CT images. The cerebral hemodynamic parameters were calculated from the restored high-quality CT images. RESULTS: In the phantom study, the average structured similarity (SSIM) value of the sequential images obtained by filtered back-projection (FBP) algorithm was 0.9438, which was increased to 0.9765 using the proposed algorithm; the SSIM values of cerebral blood flow (CBF) and cerebral blood volume (CBV) map obtained by FBP algorithm were 0.7005 and 0.6856, respectively, which were increased using the proposed algorithm to 0.7871 and 0.7972, respectively. CONCLUSION The proposed method can effectively suppress noises in low-dose cerebral perfusion CT images to obtain accurate cerebral hemodynamic parameters.
Algorithms ; Cerebrovascular Circulation ; Image Processing, Computer-Assisted/methods* ; Phantoms, Imaging ; Tomography, X-Ray Computed/methods*

PARP14 is an intracellular single AI)P-ribose transferase member of the superfamily of polyADP-ribose polymerases.PARP14 is associated with a wide range of biochemical transfor-mations in vivo through poly( ADP-ribosylation, PAKs) modifi- cation of target proteins.For example, it plays an important part in eellular transcriptional regulation, stress response, DNA re-pair, RNA splieing, mitoehondrial function, division, and nu- cleosome formation.PAR PI 4 is elosely related to the development and progression of cancers (such as hepatoeellular carcino- ma, multiple myeloma and pancreatic cancer) , metabolie diseases, and inflammatory diseases, making it a potential dnig dis- eovery target.This research reviews the structure and biological functions of PARP14, and summarizes the role of PARP14 in disease, as well as the existing PARP14 small molecule inhibitors and decompressors.It provides a brief update to the research and development of PARP14 inhibitors and decompressors to assist in the development of selective PARP14 inhibitors and decompressors.It provides reference for the research and development of drugs or chemical sensitizers targeting PARP14.

OBJECTIVETo investigate the protective effects of daidzein (DD) on ventricular remodeling in rats with myocardial hypertrophy induced by pressure overload and its mechanism.

METHODMyocardial hypertrophy model of rats induced by pressure overload was prepared by constricting abdominal aorta. The operated rats were randomly divided into sham operated control group, aorta-constricted model group and three DD groups (30, 60, 120 mg kg(-1)). Four weeks later, the heart-weight (HW), left ventricular weight (LVW), the ratio of HW/BW and LVW/BW (LVI) and the cardio-myocyte diameters (MD) after dyeing by HE colar were measured. The hydroxyroline, nitric oxide (NO) and the activity of nitric oxide synthetase (NOS) and Na+ -K+ -ATPase, Ca2+ -ATPase in left ventricle were quantified with spectrophotometry and the angiotension II (Ang II) in left ventricle and serum was messured with radioimmunoassay.

RESULTAfter treatment of the left ventricular with DD, vs aorta-contricted model group, NO content, cNOS and Na+ -K+ -ATPase, Ca2+ -ATPase activity were significantly increased, the content of AngII in left ventricle and serum and iNOS activity and the ratio of HW/BW, LVI, MD were significantly reduced.

CONCLUSIONDD has protective effects on ventricular remodeling in rats with myocardial hypertrophy induced by pressure overload and its mechanism may be related to raising NO content and reducing the level of Ang II.

Angiotensin II ; blood ; metabolism ; Animals ; Hypertrophy, Left Ventricular ; metabolism ; pathology ; Isoflavones ; pharmacology ; Male ; Myocardium ; metabolism ; pathology ; Nitric Oxide ; metabolism ; Phytoestrogens ; pharmacology ; Random Allocation ; Rats ; Rats, Wistar ; Ventricular Remodeling ; drug effects

Objective: To study the effects of Xanthnotoxol on contractility of isolated rabbit ileum and its relationship with Ca2+. Method: Routime experimental methods for isolated ileum were adopted. Result: Xanthnotoxol (XT) and Verapamil (Ver) inhibited the contraction of iso lated rabbit ileum smoth muscle induced by submaximal concentrations of acetylcholine　(ACh)　and serotonim　(5-TH), with a IC50 value (μmol*L-1) of 10.495±1.521,0.428±0.001and 18.132±1.627,0.249±0.003,respectively. XT and Ver inhibited the contraction induced by Ca2+after high K+ depolorization and for noncompetitively antagonist CaCl2 cumulative dose-response curve, the pD'2 value was 4.69±0.03 and 6.35±0.10, respectively. XT (10μmol*L-1)and Ver(0.06μmol*L-1)inhibited the contraction induced by ACh in Ca2+-free medium, while XT (100μmol*L-1)but not Ver(0.6μmol*L-1) inhibited the extracellar Ca2+-dependent contraction induced by ACh. Conclusion: XT has a calcium-antagonistic effect which was not similar to that of Ver.

Platelet-rich plasma (PRP) is a kind of autologous blood product. It is a platelet concentrate extracted from autologous blood through centrifugation or apheresis process. It is generally believed that the platelet concentration in PRP should be 4-8 times of the platelet count in the whole blood. Platelets with high concentration can release a variety of growth factors and media after activation, which is conducive to tissue repair and regeneration. PRP has been used in regenerative medicine for more than 30 years, and has achieved good results. In recent years, it has also been widely used in facial aesthetics, involving acne, skin aging, hair loss, chloasma and other fields. In this review, we are not only emphasized the preparation and use of PRP, but also outlined the application progress of PRP in facial aesthetics.

ObjectiveThe purpose of this study was to investigate the effects of transcutaneous electrical acupoint stimulation (TEAS) on cognitive function of vascular dementia (VD) rats and its mechanism. MethodsVD rat model was established by modified two-vessel occlusion (2-VO). After modeling, TEAS and electroacupuncture (EA) were used to stimulate Baihui and Zusanli points of rats respectively for 14 d. After treatment, novel object recognition test, Morris water maze test, and Y maze test were used to evaluate the spatial memory and learning ability of rats. Hematoxylin and eosin staining was used to observe the morphology of hippocampal neurons. Transmission electron microscopy was used to observe the ultrastructure of hippocampal mitochondria. Enzyme-linked immunosorbent assay kits were used to detected the levels of SOD, CAT, GSH-Px, MDA and ROS in serum of rats. Western blot was used to detect the expression of PGC-1α, TFAM, HO-1, NQO1 proteins in the hippocampus, Keap1 protein in the cytoplasm and Nrf2, NRF1 proteins in the nucleus. ResultsAfter treatment for 14 d, compared to the model group, the escape latency of VD rats decreased, while the discrimination index, the times of rats crossing the original platform area, the residence time in the original platform quadrant, and the percentage of alternation increased. TEAS can improve the structure of hippocampal neurons and mitochondria of VD rats, showing that neurons were arranged more regularly and distributed more evenly, nuclear membrane and nucleoli were clearer, and mitochondrial swelling were reduced, mitochondrial matrix density were increased, and mitochondrial cristae were more obvious. The levels of SOD, GSH-Px and CAT in serum increased significantly, while the concentration of MDA and ROS decreased. TEAS also up-regulated the expression levels of PGC-1α TFAM, NQO1 and HO-1 proteins in the hippocampus and Nrf2, NRF1 proteins in the nucleus, but down-regulated the Keap1 protein in the cytoplasm. ConclusionTEAS can improve cognition, hippocampal neurons and mitochondrial structure of VD rats, and the effect is better than EA. The mechanism may be the activation of PGC-1α mediated mitochondrial biogenesis and antioxidant stress, which also provides a potential therapeutic technology and experimental basis for the treatment of VD.

Objective The aim of this study was to explore the regulatory effect of calcitonin gene-related peptide (CGRP) on nitric oxide synthase (NOS) and the nitric oxide (NO) pathway in tibial fracture (TF) healing in the rat model of highly selective denervation.Methods A total of 60 SD rats were randomly divided into three groups of equal number, TF control, TF + sensory denervation (SD), and TF + motor denervation (MD). At 1, 2, 3 and 4 weeks after modeling, osteotylus samples were obtained from the rats for observation of the bone morphology and determination of the expressions of CGRP and NOS by immunohistochemistry and HE staining.Results At 2 and 3 weeks after modeling, the rats of the TF+SD group, as compared with the TF controls, showed significantly decreased expression of CGRP (0.150±0.014 vs 0.210±0.013, P<0.05; 0.143±0.017 vs 0.203±0.013, P<0.05) and that of eNOS in the osteotylus (0.170±0.016 vs 0.219±0.026, P<0.05; 0.158±0.016 vs 0.201±0.013, P<0.05).Conclusion Selective denervation, especially sensory denervation, may change the expression of CGRP and thereby that of NOS in the osteotylus of the rat with tibial fracture, which consequently affects the growth of the osteotylus and fracture healing as well.