Objective: To establish a HPLC method for determination of Baicalin in Xiaochaihu Tablet. Method: HPLC was used with C18 column, CH3OH - H2O - H3 PO4 (42: 58: 0. 2) as mobile phase and detection wavelength at 280nm. Results: Baicalin showed a good linear relationship at the range of 0. 2 - 1. 0ug. The average recovery was 98. 01% and RSD was 1. 07 %. Conclusion: This method is simple and accurate, and can be used for the quality conltrol of Xiaochaihu Tablets.