Objective To understand adolescent's mental stress, coping style and their correlating with emotion. Methods This research, in order to be tried with 5870 middle school students, adopt Mental Stress Questionnaire, Coping Style Scale and Profile of Mood States. Results Compared with male students, female students had higher scores on stress from school work achievement and appearance(P＜0.05) and lower scores on stress from family[(2.04±1.19)vs(2.11±1.25)](P＜0.05),also they had higher sores on extra-coping style[(3.11±0.77)vs(2.77±0.81)](P＜0.01). Compared with lower grade students, higher grade students had higher scores on all mental stress except stress from family, while they had lower scores on extra-coping style (P＜0.01). Stepwise logistic regression analysis indicated that high scores on mental stress and intro-coping style could predict negative emotion (P＜0.01), and extra-coping style could predict positive emotion (P＜0.01).Conclusion The middle school students suffered more stress from school work achievement than from other aspects. Students who differed in sex, grade had significant different scores on mental stress and coping style. Mental stress and coping style could predict adolescents' emotion.

Occupational Health

AIM: To investigate the application value of contrast -enhanced ultrasound ( CEUS) and analysis software for intra-ocular tumor diagnosis.
METHODS:Retrospective analysis for 90 cases (90 eyes) with intra- ocular tumor with CEUS examination, and all cases were confirmed by pathology after surgery or clinical comprehensive diagnosis and follow-up.
RESULTS: Choroidal hemangioma, choroidal melanoma, choroidal metastatic carcinoma had obvious different CEUS enhancement pattern, quantitative analysis indexes of those tumors by imaging analysis software were significantly different (P<0. 05).
CONCLUSION: The CEUS can provide quantitative analysis for intra - ocular tumor with preferable clinical application value.

BACKGROUND: Hippocampal formation of brain, a cerebral area related with learning and memory, is closely related to spatial cognitive activity.Peroxidative stress following the onset of cerebral ischemia can induce DNA injury in hippocampal 'area and dentate gyrus, the fall of the ability of DNA plerosis and correspondingly a decline in the function of learning and memory.OBJECTIVE: To investigate the effect of hongjingtian on the expression of nucleic acid of hippocampal area and dentate gyrus, the learning and memory area of rats with complete cerebral ischemia-reperfusion injury.DESIGN: Randomized controlled study.SETTING: Central Laboratory of Armed Police Medical College.PARTICIPANTS: The experiment was completed at the Central Laboratory of Armed Police Medical College from April 2002 to April 2004. Totally 60 Wistar male rats were selected and divided randomly into 5 groups Model group: Rats were perfused daily with distilled water of a volume the perfused daily with distilled water of a volume the same as that in medication group.METHODS: Rats in each group were perfused incessantly for 7 days.Modified Pulsinelli-4 vessel blocking method was used to reproduce the rat model of acute complete cerebral ischemia-reperfusion. Rats in sham-operation group were not treated with the operation of burning vertebral artery and clipping common carotid artery. The changes of DNA and RNA expressions in cerebral hippocampal area and dentate gyrus were observed with acridine orange staining method after model establishment.MAIN OUTCOME MEASURES: Expression of DNA and RNA in cerebral hippocampal area and dentate gyrus of rats in each group.RESULTS: All 60 rats entered the final analysis. DNA and RNA in shamoperation group distributed evenly, border of fluorescent reflex was legible and response intensity was strong. Border of DNA and RNA fluorescent reflex was illegible and response intensity was obviously weak in model ly, border of fluorescent reflex was legible and response intensity was group was not of obvious difference as compared with that in model group.CONCLUSION: The illegibility of DNA and RNA fluorescent response in operation group is related with the breakage of DNA chain induced by oxidative stress during cerebral ischemia-reperfusion injury. Border of DNA and RNA fluorescent reflex in hippocampal area and dentate gyrus is legihibit the breakage of DNA chain induced by oxidative stress and protect learning and memory function in hippocampal area and dentate gyrus of rats with complete cerebral ischemia-reperfusion.

Objective To discuss the application of surface anatomy scanning in the department of cerebral surgery. Methods 24 cases were examined by surface anatomy scanning.These included 5 normal cases,10 gliomas,4 metastasis tumors,3 meningiomas,1 cavernous hemangioma and 1 cerebral infarction.Results 5 normal cases with surface anatomy scanning showed excellent visualization of the sulci and gyri on the surface of the brain.In 19 cases with cortical or subcortical lesions,14 cases showed the relationship between the lesion and the brain surface structures well.5 cases could not displayed because of server edema around the lesion.Conclusion The brain surface anatomy scanning is useful for the localization of cortical and subcortical pathology,for the diagnosis of the disease of sulci and gyri,can partly replace the navigation.

Inflammatory mediators releasing during acute and chronic diseases can activate multiple intracellular signals inducing cascades of the mitogen-activated protein kinase(MAPK) signalling transduction pathway. MAPK plays a significant role in the recruitment of leukocytes to the inflammation sites. Stimulation of leukocytes by pro-inflammatory cytokines is known to activate the MAPK isoform p38. However, the consequences of p38MAPK activation during leukocyte recruitment, adhesion, migration, functions of effector(oxidative burst), and the network of cytokines mediated by p38MAPK are all investigated for further elucidation. Specific p38 inhibitors in reducing the production of inflammatory mediators and providing more effective treatment are now being developed.

Objective To study the MRI features of sporadic Creutzfeldt-Jakob disease(sCJD). Methods Three patients with clinically diagnosed sCJD underwent MR study,including SE T_1WI,FSE T_2 WI,and DWI sequences.The MR imaging features were analyzed.Results The lesions were not definite either in SE T_1 WI or in FSE T_2 WI,but were prominent in DWI.Abnormal hyperintensive signal appeared in the cerebral cortex,with the frontal,parietal,and occipital lobes being the mostly involved region.The subcortical white matter was normal.The bilateral caudate nuclei and thalami could also be involved.The abnormal signal could be either symmetrical or asymmetrical.There was diffuse atrophy of the brain parenchyma in the late phase of disease,especially in the cortex.Conclusion With the application of MR study,especially the DWI,combined with its characteristic clinical manifestation,the diagnosis of sCJD can be made definitely.

OBJECTIVE:To analyze the clinical application and trend of antitumor drugs in 42 hospitals from Beijing,Shang-hai and Chengdu,and to provide reference for rational drug use in clinic. METHODS:The utilization of antitumor drugs in 42 hos-pitals from Beijing,Shanghai and Chengdu during 2012-2016 was analyzed statistically in respects of consumption sum,DDDs. RESULTS:The consumption sum of antitumor drugs in 3 cities,Beijing ranked the first;annual growth rate of Shanghai was the highest. In the list of consumption sum of antitumor drugs of 3 cities in 5 years,immunosuppressive agents as tacrolimus,mycophe-nolate mofetil were among top 10 antitumor drugs in the list of consumption sum. Top 1 antitumor drug in the list of consumption sum in Beijing,Shanghai and Chengdu were thymopentin,tacrolimus and thymalfasin,respectively. Among subtype of antitumor drugs,other antitumor drugs and adjuvant drugs in Beijing and Chengdu during 2012-2014 took up the first place in the list of con-sumption sum;the consumption sum of antimetabolite during 2015-2016 occupied the first place,and that of Beijing was higher than that of Chengdu. The cost of antimetabolite in Shanghai during 2012-2016 was the highest. The consumption sum of other antitumor drugs and adjuvant drugs were the highest in 3 cities during 2012-2014;that of antimetabolite was the highest during 2015-2016. Among related drugs,immune enhancer,immunosuppressive agents and analgesics ranked the top in the list of consumption sum. Among top 10 antitumor drugs in the list of DDDs,most were antineoplastic medicine,and only cyclophosphamide among alkylat-ing agents entered the ranking. CONCLUSIONS:The growing trend of antitumor concumption sum is stable. Antimetabolite,hor-mones and plant-derived antitumor are becoming larger. Molecular targeted therapies have a good prospect for application.

Objective To summarize the technique and evaluate the effect of anesthesia for off-pump coronary artery bypass surgery (OPCAB). Methods From 1997 through March of 2005, five hundred and forty-three consecutive patients undergoing OPCAB were retrospectively studied. Anesthesia was induced with midazolam 0.05～0.12mg/kg, ketamine 0～0.5mg/kg and fentanyl 5～15?g/?kg. Anesthesia was maintained with isoflurane 0.3%～1.5% and oxygen, combined with propofol 1～3mg/(kg?h). Intraoperative hypotension resulting from either surgical procedure or anesthetics should be dealt with inotropes immediately. Results Intraoperative hemodynamics deviation was tolerated with the support of inotropics. The mean extubation time was 5.8?1.6h. The ICU stay was 0.5 to 2.0 day. The patients were discharged in 8.0?1.2 days. Two patients met sudden death in the second postoperative day. Conclusion Rational use of anesthetics and inotropics to stabilize hemodynamics during operation plays a key role in successful OPCAB.

Objective To investigate the feasibility whether clearance of iohexol can be a reliable, sensitive and safe method for the determination of GFR. Methods The GFR of 19 patients with different renal functions were examined using clearance of 99m Tc-DTPA and clearance of iohexol. Then the correlation of them was analyzed. Serum and urinary iohexol was determined by X-ray fluorescence analysis. Results, These two methods were significantly correlated (r = 0. 98). Conclusion Clearance of iohexol is a safe, comfident, no-radioactivity method for the clinical practice of GFR determination.