Objective: To study the chemical constituents from the root bark of Changium smyrnioides. Methods: Compounds were isolated by various kinds of column chromatographies on silica gel, Sephadex LH-20, and recycling preparative HPLC from the ethanol extract in the root bark of C. smyrnioides, and their structures were elucidated by the physicochemical characteristics and spectral analyses. Results: Fifteen chemical constituents were obtained and identified as imperatorin (1), phellopterin (2), xanthotoxol (3), 5-hydroxy-8-methoxy-psoralen (4), vanillic acid (5), alloimperatorin (6), psoralen (7), bergapten (8), 8-O-β-D-glucopyranosyl- 5-methoxylpsoralen (9), isopimpinellin (10), caffeic acid (11), aurantiamide acetate (12), vaginatin (13), β-sitosterol (14), and succinic acid (15). Conclusion: Compounds 6-13 are isolated from the plants in Changium Wolff for the first time.

Adult ; Bronchoalveolar Lavage ; Bronchoalveolar Lavage Fluid ; cytology ; Humans ; Macrophages ; pathology ; Male ; Middle Aged ; Pneumoconiosis ; pathology ; therapy

Objective To investigate the in vitro inhibitory effect of LIGHT on human esophageal carcinoma cells. Methods LIGHT Fc expression vector was transfected into human esophageal squamous carcinoma cell line, Eca109. The inhibitory effect of LIGHT gene on cell growth was detected by MTT and cell growth curve. The expressions of LT?R and HVEM were detected by RT PCR. Results Expression of LIGHT Fc gene could inhibit Eca109 cell proliferation. The growth curve of Eca109/LIGHT was significantly lower than that of the control group in the culture medium containing 1% FCS. MTT test showed that there was significant difference in cell viability between Eca109/LIGHT and the control group ( P

Objective To evaluate the techniques and effects of post-operational surgery of colorectal cancer for metastatic tumor on caudate lobe of liver. Methods For the patients admitted from Jan. 1999 to Jan. 2007 to the Navy General Hospital of PLA and undergone metastasectomy on caudate lobe of liver after colorectal cancer operation, the operative procedure and effects were studied retrospectively. Results A total of 18 cases of metastasectomy on caudate lobe of liver were successfully performed. The mean diameter of the metastases was 6.3cm (3.2-11.3cm). The types of operation included total caudate lobectomy (6 cases), total caudate lobectomy combined with extended hepatectomy (9 cases) and partial caudate lobectomy (3 cases). The mean operative time was 198min (154-360min) and the average intraoperative blood loss was 975ml (400-3250ml). No intraoperative death occurred, while the remarkable complications were found in 5 cases (27.8%). The survival rates of follow up for 1, 3 and 5 years were 83.3%, 61.1% and 27.8%, respectively. Conclusions The metastases on caudate lobe of liver after colorectal carcinoma resection usually involve all the hepatic portals. Surgical resection, though sophisticated in technique, remains to be a safe and effective treatment, and the first choice for treating metastases.

Objective: To analyse the effects of CVP chemotherapy through arterial injection (CVPC) in the treatment of squamous cell carcinoma (SCC) of maxillary sinus.Methods:39 cases of primary squamous cell carcinoma of maxillary sinus at stage Ⅳ (M0) were treated with following regimen : carboplatin 300～350 mg/m 2 ia gtt on d1, etopside 100 mg ia gtt on d1～5, pingyangmycine 8 mg ia gtt on d1～5. The drugs were given through superficial temporal artery at the level of internal maxillary artery. Gave one cycle treatment before operation which was practised 4 weeks later.Results:Partly remission was found in all patients after chemotherapy. Pathological margin of original focus was negative after operation. 5 cases lost follow up. 4 cases were dead of distant metastasis (after 5,7,8 and 12 months respectively). 30 cases were alive without tumour. 17 cases were over 5 years, 6 over 3 , 4 over 1 and 3 less than one year.Conclusion: CVP arterial injection chemotherapy is effective in the treatment of SCC of maxillary sinus at late stage.

OBJECTIVE:To establish the quality criteria of Kesuting oral liquid. METHODS: Qualitative identification of Herba Ephedrae,Folium Mori and Radix Et Rhizoma Glycyrrhizae was conducted using TLC method. The content of Baicalin was determined by HPLC in which the chromatographic separation was performed on Spherigel ODS C18(250mm?4.6mm,5?m) column with mobile phase consisted of methanol-0.4%phosphate solvent(52∶48) at a flow rate of 1.0 mL?min-1. The UV wavelength was set at 280 nm. RESULTS: The TLC spots of Herba Ephedrae,Folium Mori and Radix Et Rhizoma Glycyrrhizae were characteristic and obvious, and which can be used for qualitative identification. The linear range of Baicalin was 0.24～1.20 ?g (r=0.999 8) with a mean recovery rate of 98.72%(RSD=1.02%,n=6). CONCLUSION:The establish criteria are suitable for the quality control of Kesuting oral liquid.

Quantitative determination of polysaccharides in Pine cones by phenol-sulfuric acid mehod was studied. and the effects of concentration of phenol-sulfuric acid and reaction temperature on color formation were investigated. It was found that the absorbance is linearly correlated to polysaceharide concentrations between 10～78?g/ml (r=0.9999). The analytical recovery was 99.8%, CV% was 1.2% and the minimal detectable concentration was 5?g/ml.

Objective To investigate the feasibility and safety of laparoscopic radical resection for rectal carcinoma.Methods A total of 80 patients with rectal carcinoma received laparoscopic radical resection in our hospital from March 2004 to January 2008.During the operation,adequate proximal and distal bowel segments,and mesentery and lymph nodes surrounding the tumor were resected completely.And then lower anterior resection(Dixon)and sigmoid colostomy for abdominal perineal resection(Miles)were implemented.Results Among the 80 patients,4 were converted to open surgery during the operation,and the other 76 patients were treated by laparoscopy successfully.Dixon surgery was performed on 44 cases with a mean operation time of(178?38)minutes,intraoperative blood loss of(61?17)ml,excised lymph nodes number of(11.3?2.9),and postoperative hospital stay of(7.1?3.2)days.Miles were carried out on 32 patients,in which the mean operation time was(231?49)minutes and intraoperative blood loss was(210?178)ml;a mean of(12.9?3.7)lymph nodes were resected,the postoperative hospital stay was(9.3?4.1)days.No residual cancer was found in the patients.After the treatment,1 patient developed stoma stenosis,5 showed perineal wound infection,1 had anastomotic leak,and 6 complained of urinary retention immediately after the surgery.No patient had postoperative hemorrhage or died after the procedure.Follow-up was achieved in 70 cases for 9 to 48 months,during which 5 patients had recurrence,2 died of multiple organ failure induced by multiple metastasis,and 5 patients developed ileus.Conclusion Laparoscopic resection for rectal carcinoma is safe and feasible for selected patients,when the surgeons are skilled with experience on both open colectomy and laparoscopic surgery.

Objective To study the efficacy of complement depletion with CVF in preventing a-cute rejection after heart allotransplantation in inbred strain rats. Methods Inbred male Lewis rats were used as recipients and Brown-Norway rats as donors, and the heterotopic heart transplantation model was established. The allografts were divided into 2 groups (n=8 in each group). After infusing low-dose CVF 20?g/kg to the CVF-treated group, cardiac allograft survival time was observed on 4 rats of each group. The remaining 4 rats in each group were killed respectively at day 1,3,5 and 6, the pathological grade for acute rejection, the complement activity in serum, the deposition of C3 on tissue, and the extent of infiltration by CD3+ T cells were compared. Results The mean survival time of heart allograft was (11. 69?0. 72) days and (6. 65?0. 35) days in CVF-treated group and control group respectively (P

Objective To discuss the resection technique for pancreatic carcinoma in patients with tumour(invasion) to the portal and/or superior mesentery vein,and the clinical significance of palliative resection of pancreatic carcinoma.Methods The clinical data of 71 cases of pancreatic carcinoma operated on within a period of 3 years were retrospectively reviewed.In patients with tumour invasion to the portal vein,a method of direc transection of pancreas was used in resection of the tumor,and was combined with local radiotherapy and chemotherapy.Results The resection rate was 57.75%.The complication rate was 22.54%.One patient died of multiple organ failure resulting from pneumonia.The survival rate at 6m,1-and 2-years was 100%,81.69%,and 40.85% respectively.Conclusions The direct transection method of pancreas(resection) is a simple and safe method to increase resection rate and prolong survival time for patients with(advanced) pancreatic carcinoma.