BACKGROUND: Decrease of regional cerebral blood flow (rCBF) perfusion of different degree exists in depressive patients, but the decreased ranges were different, different cerebral cortical and subcortical regions are involved.OBJECTIVE: To observe the types of abnormal rCBF perfusion in patients with depressive disorder.DESIGN: A diagnosis-based case-control analysis.SETTINGS: Department of Nuclear Medicine, Ruijin Hospital Affiliated to the Medical College of Shanghai Jiaotong University; Staff Room of Biomedical Engineering, Medical College of Shanghai Jiaotong University;Putuo District Mental Health of Shanghai City.PARTICIPANTS: The patients with depressive disorder were selected from the Putuo District Mental Health Center from January 1999 to December 2000, and 19 normal subjects matched by age were selected from the medical staff of the hospital as normal controls.METHODS: The patients with depressive disorder and healthy subjects were examined by single photon emission computerized tomography (SPECT), ethyl cysteinate dimmer (99Tcm-ECD) was performed, and the images were analyzed with the statistical parametric mapping (SPM). All the SPECT images were converted into DICOM format or ANALYZE-7 format at first, and then the images were standardized by taking MRI template as the template and smoothed, and the significantly different regions were evaluated by the statistical analysis and inferences with the Gaussian random theory.MAIN OUTCOME MEASURES: The differences of the cerebral blood flow perfusion in specific region and the changes of average rCBF images were mainly observed in the depressive disorder group and normal control group.RESULTS: All the 12 patients with depressive disorder and 19 normal healthy subjects entered the analysis of results. The SPM showed that the blood flow perfusion was decreased in bilateral frontal lobes, left superior temporal gyrus, left Brodmann area 20, left mamillary body of thalamus,right cingulated gyrus and right putamen in the patients with depressive disorder. The blood flow perfusion was increased in the right central frontal gyrus, right superior temporal gyrus, right occipital lobe and left central frontal gyrus.CONCLUSION: The rCBF is obviously changed in the frontal lobe, temporal lobe and limbic system of patients with depressive disorder.

OBJECTIVETo develop a chitosan (CH)/polyethylene glycols succinate acid (PEG-SA)-mediated mitomycin C (MMC) delivery system and investigate its drug release characteristics in vitro and its effect against scar tissue adhesion in vivo.

METHODSMitomycin C loading in the composite CH/PEG-SA/MMC films was determined using ultraviolet. The freeze-dried films were dispersed in 1 ml PBS (pH7.4) and mitomycin C release in vitro was determined according to the mitomycin C concentration-UV value standard curve. The influence of the film structure on the drug release was evaluated. The drug delivery system was then implanted in SD rats, and 4 weeks later, immunohistochemical and histological examinations were carried out to assess the therapeutic effect on epidural scar tissue.

RESULTSThe linear regression equation of the mitomycin C concentration-UV value standard curve was y=0.593x(3)-2.563x(2)+25.944x-0.236 (R(2)=1.000). The film demonstrated good drug delivery capability, and 20 mg of the samples in PBS showed a peak mitomycin C release after 12 days of 14.9616 microg/ml, which was higher than the ID(50) of mitomycin C (10.4713 microg/l) to the fibroblasts. On days 18 and 32, another two drug release peaks occurred (14.4824 microg/ml and 11.4092 microg/ml, respectively), followed by maintenance of slow release. Till day 60, the accumulative mitomycin release reached 0.1793 microg/ml, and the loaded drug was ultimately completely released. Significant differences were noted in the hydroxyproline content in the scar tissues of different groups (F=12.085, P=0.000), and the CH/PEG-SA/MMC DDS reduced the amount of scar tissue and promoted its orderly alignment to control potential scar hyperplasia that may compress the spinal cord and nerve roots.

CONCLUSIONThe composite film for drug delivery possesses good flexibility and mechanical properties and allows sustained drug release of mitomycin C to prevent epidural scar tissue adhesion following lumbar laminectomy.

Animals ; Chitosan ; chemistry ; Drug Delivery Systems ; Intervertebral Disc ; drug effects ; physiopathology ; surgery ; Mitomycin ; administration & dosage ; chemistry ; Polyethylene Glycols ; chemistry ; Polyethylenes ; chemistry ; Rats ; Rats, Sprague-Dawley ; Succinates ; chemistry ; Tissue Adhesions ; prevention & control

Objective To anderstand the metagenesis of Oncomelania snails in the mountainous regions so as to control the spread of snails and the epidemics of schistosomiasis.Methods Observation spot was established at a typical snail habitat close to Puge county,Sichuan province from February 2008 to July 2009.Random sampling was applied to determine the place of each frame during the observation.All the snails in each frame were collected and numbers counted in the laboratory,with the number of mating pairs in each frame also observed.Snails being collected were measured for the body indices and the dissection was carried out to identify gender composition.survival status and the number of live snails in each frame counted.Line graphs of the body indices.mating pairs in each observed months,bar graphs of the snail density,proportions of gender together with the maturity of the snails in each month were drawn.Results The number of live snail existed the whole year and its density fluctuated.All the three kinds of body indices showed the same time trend and a dynamic circulation.The young snail existed all year around and arose constantly in proportion from May,becoming the dominant snailin October to replace the adult snails.The young and adult snails also showed a dynamic alternative.The gender composition showed no significant difference during each month.The number of the mating pairs was more on April.May and June annually,when were the snail's main multiplying stage.Conclusion In mountain area.the young snails existed through all the year while adult snails appeared to be dominantin each month except for October.Oncomelania snail showed a circular process of metagenesis which started in May and finished in October.The snail population presented a dynamic equilibrium.It was concluded that ecological studies on Oncomelania snail were extremely relevant,either to optimally apply the existing control measures or to develop alternative measures for snail control,ecologically or biologically.

Objective To evaluate the efficacy and safety of edaravone combined with cattle encephalon glycoside and ignotin in the treatment of severe traumatic brain injury .Methods A total of 74 cases with severe traumatic brain injury were divided into control group ( n =37 ) and treatment group ( n =37 ) .Control group was given cattle encephalon glycoside and ignotin injection 10 mL, intravenous infusion, qd.Treat-ment group was received edaravone 30 mg, intravenous infusion , qd, on the basis of control group .Two groups were treated for two cycles with 28 d per cycle.The clinical efficacy , brain ede-ma area, the scores of acute physiology and chronic health evaluation Ⅱ(APACHE-Ⅱ), glasgow coma scale(GCS) and activities of daily living scale (ADL), levels of glial fibrillary acidic protein (GFAP), S100 calcium -binding protein( S100β) , heme oxygenase 1 ( HO -1 ) , matrix metalloproteinase -9 ( MMP -9 ) , hematocrit ( HCT ) and erythrocyte sedimentation rate ( ESR ) and incidence of adverse drug reactions were compared in two groups . Results After treatment, the total effective rate in treatment group was 94.59%(35/37), which was significantly higher than that in control group [ 81.08%( 30/37 ) , P<0.05 ] .There was no significant difference of the HCT in control group after and before treatment [(44.30 ±5.19)%vs (46.03 ±5.50)%, P>0.05], and the level of HCT in treatment group after treatment was significantly lower than that before treatment [ ( 40.15 ±4.44 )% vs (46.15 ±5.47)%, P<0.05].After treatment, the main observation indexes in treatment and control group: brain edema area were ( 12.46 ±1.42 ) , ( 18.46 ±1.96 ) cm2; APACHE -Ⅱ score were ( 10.19 ±1.13 ) , ( 13.57 ± 1.52 ) point;serum GFAP were ( 6.54 ±0.67 ) , ( 13.77 ±1.42 ) ng? L-1; serum S100 βwere ( 0.07 ±0.01 ) , (0.09 ±0.01) μg? L-1; serum HO -1 were (4.73 ±0.49), (11.74 ±1.25)μg? L-1; serum MMP -9 were (137.67 ±15.53), (167.65 ±17.53)g? mL-1 and ESR were (3.01 ±0.33), (3.88 ±0.48)mm? h -1, also those indexes in treatment group after treatment were significantly lower than those in control group (P<0.05).After treat-ment , among the treatment and control group , the scores of GCS were ( 15.33 ±1.62 ) , ( 9.63 ±0.98 ) point and scores of ADL were (57.24 ±6.01), (46.35 ±4.72) point, and those indexes in treatment group after treatment were significantly higher than those in control group ( P<0.05 ) .The adverse drug reactions were based on abnormal liver function, nausea, vomiting and rash for two groups.Also, the incidences of adverse drug reactions in treatment and control group were 8.11%, 18.92%, respectively , without significant difference ( P > 0.05 ) . Conclusion Edaravone combined with cattle encephalon glycoside and ignotin have a definitive clinical efficacy and safety for the treatment of severe traumatic brain injury .

Objective To evaluate the vitamin D status of residents in two cities of Zhejiang province.Methods 442 participants from Hangzhou and Ningbo were selected using multi-stage cluster random sampling method,then the blood samples were collected and the serum level of 25 -hydroxyl vitamin D [25 (OH)D]was detected by the method of radioimmunoassay to analyze the Vitamin D status.Results Median (25 th-75 th percentile)level of serum 25 (OH)D in subjects aged 6 -11,12 -17,18 -44,45 -59 and over 60 years were 53.19 (41.85 -64.89),48.76 (32.56 -60.52),59.91 (48.05 -72.79),68.67 (55.50 -78.07)and 70.97 (56.91 -84.65)nmol/L,respectively.The number of vitamin D deficiency [25(OH)D<25 nmol/L],insufficiency[25≤25(OH)D <50 nmol/L],normal [50≤25 (OH)D <75 nmol/L ],sufficiency [25 (OH )D ≥75 nmol/L ] were 2.95%,30.84%,44.22% and 22.00%, respectively.Conclusion The deficient and insufficient status of vitamin D is common among residents in two cities of Zhejiang province.Vitamin D nutrition situation should be improved.

Objective To detect and analyze the clusters of schistosomiasis on marshland and lake areas based on geographic information system (GIS) in 2008 and to provide suggestions for the development of integrated methodology on the detection of clusters on related diseases. Methods Moran' s I of global spatial autocorrelation, Getis-Ord Gi of local spatial autocorrelation and SaTScan were used to detect the schistosomiasis clusters based on GIS and comparison of the results for different methods were performed. Results Results from the global Moran' s I tests for all the marshland and lake areas related to the schistosomiasis were statistically significant (P＜0.05)and indicated spatial heterogeneity; the z-score outcomes as calculated by Getis-Ord Gi indicated high cluster that 50 clusters were categorized at the 0.05 significance level and the z-score of these 45 clusters were more than 0. Results of SaTScan statistics appeared the same as local spatial autocorrelation and almost showing the existence of 5 cluster areas. Conclusion The geographical distribution of clusters where schistosomiasis was prevalent showed that it was closely corresponded to the middle and lower Yangtse river and in particular, many clusters were located near the boundary of Hubei and Hunan province.

To study Ginkgo biloba leaves in different producing area, we establish an HPLC method for the simultaneously determination of seven flavonoids glycosides and four biflavonoids in G. biloba leaves. The analysis was performed on an Agilent ZORBAX SB-C₁₈ column(4.6 mm×250 mm, 5 μm) wich acetonitrile, and 0.4% phosphoric acid as mobile phase at flow rate of 1 mL•min⁻¹ in a gradient edution, and the detection was carried out at 254 nm.The calibration curves of the seven flavonoids glycosides and four biflavonoids had a good linearitiy with good recoveries. The established HPLC method is simple, rapid, accurate, reliable, and sensitive, and can be applied to the identification and quality control of G. biloba leaves.

OBJECTIVETo explore the spatial distribution and elimination of Oncomelania hupensis in mountainous regions.

METHODSPuge County in Tezi township was selected as the study site and the quadratus were placed randomly to investigate snail. The two sods with water were selected as the sites of snail elimination. One sod with area of 1000 m2 and mean density of 9.88 snails/0.11 m2 was selected as the trial group with 'heaping' method, and the other with area of 1000 m2 and mean density of 9.80 snails/0.11 m2 as the control group with sprinkling method. The molluscacidal effect of the two methods was compared by systematic sampling (5 m x 5 m). The sample size was 40 quadratus.

RESULTSThe snail distributed mainly in the sods with water, canals and farmlands. Among the three snail habitats, the area with snail was the most in the farmlands with relatively lower density of living snail; the next was the sods with water, with relatively higher density of living snail. Before killing snails, the rate of quadratus with snails was 87.50% in the trial site, and 82.50% in the control site. The mortality of snails was 3.89% in the trial site, and 4.16% in the control site. After three months, no living snails were found in the trial site, while in the control site, the rate of quadratus with snails (chi2 = 0.31, P > 0.05) and the mortality of snails (chi2 = 3.12, P > 0.05) did not decrease significantly, and the density of living snails only reduced by 8.88%.

CONCLUSIONThe 'heaping' method is an efficacious measure for snail control.

Animals ; China ; Environment ; Molluscacides ; Pest Control ; Schistosoma japonicum ; Schistosomiasis japonica ; prevention & control ; Snails ; parasitology

China ; epidemiology ; Cholera ; epidemiology ; Disease Outbreaks ; Humans

OBJECTIVETo analyze retrospectively 1018 patients who underwent coronary artery bypass grafting surgery (CABG) in order to summarize surgical techniques and clinical outcome.

METHODSFrom 1997 through 2004, data of same surgeon for 508 patients who underwent conventional coronary artery bypass surgery on pump (CCABG) and 510 patients who underwent off-pump CABG (OPCAB) were collected and analyzed retrospectively. Eight hundred and fifty-two patients had unstable angina, 582 patients were over 60 years old (57.2%) and 784 patients had concomitant diseases including valve lesion, hypertension, diabetes, myocardial infarction, left ventricular aneurysm with septal defect, stroke, chronic obstructive pulmonary diseases, renal failure and cancer. A hundred and fifty-six patients had left main stem (LIMS) stenosis and 671 patients, triple-vessel disease.

RESULTSTotal mortality was 0.39% (4-case death) and morbidity, 1.6% (sternal dehiscence, stroke and mediastinitis). The grafts per patient with CCABG and OPCAB were 3.3 +/- 0.6 vs. 2.5 +/- 0.4. Left internal mammary artery use was 93.8% of the patients, 29 patients were implanted intra-aortic balloon pump intraoperatively. Follow-up was 4 months to 7 years.

CONCLUSIONSScientific surgical strategies, excellent surgical techniques and improvement of cardiac anesthesia and cardiopulmonary bypass make the mortality and morbidity decrease significantly, CABG surgery is safe and effective in patients with coronary artery disease.

Aged ; Cardiopulmonary Bypass ; Coronary Artery Bypass ; methods ; mortality ; Coronary Artery Bypass, Off-Pump ; Coronary Disease ; mortality ; surgery ; Female ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Treatment Outcome