2.Ultrastructure of Vascularization During Osteogenesis by Tissue Engineering Technique
kai-gang, ZHANG ; bing-fang, ZENG ; chang-qing, ZHANG
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(02):-
Objective To observe the changes of osteoblasts and vascularization during osteogenesis by tissue engineering technique under the electron microscope and study the feasibility of improving vascularization of the tissue engineering bone by using the small intestine submucosa(SIS) as the scaffold. Methods The bone mesenchymal stem cells(BMSCs) were isolated by using the gradient centrifuge method.BMSCs were seeded in the SIS.The scaffold-cell constructs were cultured in vitro for two weeks.There were no cells on the SIS as control.They were implanted subcutaneously in the dorsa of the athymic mice.The implants were harvested after(in vivo) incubation for 4,8 and 12 weeks.The changes of osteoblasts and vascularization were observed under the transmission electron microscope and the scanning electron microscope. Results The BMSCs grew quite well.BMSCs differentiated on the surface of the SIS and secreted a great deal of extracellular matrices.The scaffold-cell constructs formed a lot of bone and vessels in vivo.The scaffold degraded after 12 weeks.No osteoblasts but vascularization and fibroblasts were observed as control. Conclusion SIS can be used as the scaffold for constructing tissue engineering bone as it can improve the formation of bone and vessels in vivo.
3.Research progress of trans-cinnamaldehyde pharmacological effects.
Li-qing ZHANG ; Zhan-gang ZHANG ; Yan FU ; Ying XU
China Journal of Chinese Materia Medica 2015;40(23):4568-4572
Trans-cinnamaldehyde, the main component of volatile oil from cassia twig or Cinnamomum cassia, which is a traditional Chinese herbal medicine. Trans-cinnamaldehyde is a kind olefine aldehyde of organic compounds and has many pharmacological properties, such as anti-inflammatory, anti-tumor, anti-bacterial, antidiabetic, anti-obesity, and neuroprotection etc. The compound has preventive and therapeutic effects on the nervous system, cardiovascular, cancer, diabetes and other diseases. Trans-cinnamaldehyde, as a preventive care of nature medicine, has great clinical and market potential. This paper gives a review about the pharmacological effects and mechanism of trans-cinnamaldehyde researched in the latest five years. We hope to provide some basic information for further research on trans-cinnamaldehyde.
Acrolein
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analogs & derivatives
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chemistry
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pharmacology
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Animals
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Cinnamomum aromaticum
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chemistry
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Humans
4.Quality of sleep in middle school teachers.
Gang CHENG ; Xiao-qing ZHANG ; Mian-zhen WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2009;27(12):757-759
Adult
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Faculty
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Female
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Humans
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Male
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Middle Aged
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Sampling Studies
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Sleep
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Surveys and Questionnaires
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Young Adult
5.Research progress in HIV auxiliary proteins counteracting host restriction factors.
Qian-Qian CHEN ; Qing-Gang XU ; Chi-Yu ZHANG
Chinese Journal of Virology 2014;30(1):84-90
Identification and functional analyses of antiviral restriction factors in hosts have become hot research topics. Four HIV restriction factors, APOBEC3G, Trim5alpha, Tetherin, and SAMHD1, have been identified in recent years. By encoding auxiliary proteins, lentiviruses can counteract host restriction factors. For example, the auxiliary proteins Vif, Vpu, and Vpx of HIV antagonize APOBEC3G, Tetherin, and SAMHD1, respectively. Furthermore, these auxiliary proteins enable the entry of HIV into host cells and influence the replication and pathogenicity of HIV. In this paper, we review the research progress in the functions of the three HIV auxiliary proteins that can antagonize the host restriction factors.
Animals
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HIV
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metabolism
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physiology
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Host-Pathogen Interactions
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Humans
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Viral Proteins
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metabolism
6.Preliminary exploration of the frontal-cingulate functional connection during identifying sad facial expression in depressed patients
Gang LIU ; Qing LU ; Jing ZHANG ; Haiyan LIU ; Zhijian YAO
Chinese Journal of Behavioral Medicine and Brain Science 2010;19(12):1057-1059
Objective To analyze the difference of frontal-cingulate functional connection between patients with major depression and healthy controls in recognization of sad facial expression, and discuss the neutral basis of emotional bias in preliminary. Methods 12 female patients with major depression and 12 matched healthy controls were scanned. The intensities of brain activation in the process of sad and neutral facial expression recognition were analyzed, and the strengths of frontal-cingulate functional connection were compared between the two groups based on dynamic causal modeling. Results Comparing the stimulus adjusting connection models with Bayesian model indicated that model Ⅱ matched with the observation data better. In the comparison between model Ⅱ and model I ,the average Bayes factor was more than 7.38. In the comparison between model Ⅱ and model Ⅲ,the average Bayes factor was more than 2.71. Calculating the intrinsic connection parameters and stimulus adjusting connection parameters based on intrinsic connection model and stimulus adjusting connection model( model Ⅱ ), the result showed that connection strength between anterior cingulate and right inferior frontal gyrus in depression was greater than that in healthy controls. Conclusion The frontal-cingulate functional connection of patients with major depression is increased abnormally ,which may be the neural basis of negative emotional bias.
7.Construction and identification of a lentiviral vector of RNA interference containing human Notch-1 gene.
Qing-qing ZHANG ; Senlin ZHANG ; Yinglan ZHU ; Zhen DONG ; Gang CAO ; Wei CHEN
West China Journal of Stomatology 2014;32(3):267-272
OBJECTIVETo construct and identify a lentiviral vector of RNA interference targeting human Notch-1 gene.
METHODSTo determine the Notch-1 gene sequences, three RNAi target sequences (shRNA1-3) were designed in accordance with the RNAi sequence design principles and cloned into the lentiviral vector pLenOR-THM by endonuclease BamH I restriction, EcoR I double digestion, and T4 DNA-ligase ligation. After the transformation into competent DH5alpha bacteria, the candidate clones were identified by Kpn I and EcoR I double digestion and DNA sequencing. The recombinant and three packaging plasmids were co-transfected into human embryonic kidney cell line 293T cells by lipofectamine to produce the lentiviral particles. The viral titer was determined. The 293T cells were infected by the lentiviral particles obtained, and transfection efficiency was assessed using a fluorescent microscope. The lentiviral vector particles were also transfected into ACC-M cells. The Notch-1 expression in the transfected cells was assayed by quantitative reverse transcription polymerase chain reaction (QRT-PCR) and Western blot analysis.
RESULTSThe lentiviral RNAi vector pLenOR-THM-Notchl for Notch-1 gene was constructed successfully. Strong green fluorescence was observed in the 293T cells under fluorescent microscope after co-transfection of the cells with the four plasmids of lentiviral vector. The virus in the supernatant reached a titer of 5.8 x 10(8) TU x mL(-1). The transfection efficiency of the collected virus exceeded 90% in 293T cells with 1 as a multiplicity of infection. The third lentiviral vector was found to significantly inhibit the Notch-1 expression at the mRNA and protein levels.
CONCLUSIONThe lentiviral RNAi vector of Notch-1 has been successfully constructed and identified.
Cell Line ; Genetic Vectors ; Humans ; Lentivirus ; Plasmids ; RNA Interference ; RNA, Messenger ; RNA, Small Interfering ; Receptor, Notch1 ; Transfection
9.Effects of dexmedetomidine on some hemodynamic indexes and consumption of total intravenous anesthetics of laparoscopic hysterectomy
Xiaojing LV ; Gang LI ; Zhongyu LIU ; Qiuyue ZHANG ; Jie ZHANG ; Qing YANG
Clinical Medicine of China 2016;32(3):217-219
Objective To observe the effects of dexmedetomidine on some hemodynamics indexes and the total dosage of propofol and remifentanil of laparoscopic total hysterectomy.Methods Sixty cases patients with total intravenous anesthesia in laparoscopic total hysterectomy were randomly divided into two groups,30 cases in each group.After induction of anesthesia,in the dexmedetomidine group,10 min before anesthesia induction pumping load dexmedetomidine 0.5 μg/kg,and then continue pumped into the maintenance dose of 0.2 μg/(kg · h),stop the medicine in 40 min before the end of operation.The control group were given the same capacity normal saline.The heart rate (HR),systolic blood pressure (SBP),mean arterial pressure (MAP),the total dosage of propofol and remifentanil at different time of before anesthesia(T0),tracheal intubation (T1),beginning of the operation (T2),extubation (T3) were recorded.Results Compared with the control group,hemodynnmic stability(HR,SBP,MAP) of each time of the dexmedetomidine group were better than the control group's,there were statistically significant differences (P<0.05).The total dosage of propofol,remifentanil of the dexmedetomidine group were (0.11.3 ± 0.013) mg/(kg · min),(0.098 ± 0.009) μg/(kg · min),of the control group were (0.151±0.012) mg/(kg · min),(0.131±0.011) μg/(kg · min),the total dosage of dexmedetomidine group was less than the control group's,there were significant differences(P=0.038,0.045).Conclusion Small dose of dexmedetomidine used in laparoscopic total hysterectomy patients can reduce the total dosage of propofol and remifentanil,more stable hemodynamics at each point,and can be used as a viable programme for clinical application.
10.Morphological study of co-culture of Schwann cells with small intestinal submucosa in vitro
Yan SU ; Chang-Qing ZHANG ; Kai-Gang ZHANG ; Xue-Tao XIE ; Bing-Fang ZENG ;
Chinese Journal of Microsurgery 2000;0(02):-
Objective To observe adhesion and growth of Sehwann cells(SCs) on small intestinal submueosa(SIS) and study the bioeompatibility of SIS with SCs.Methods The SCs of SD neonate rat were isolated and cultured in vitro,then were seeded on prepared SIS.At different times,the adhesion,growth and proliferation of SCs on SIS were observed by phase contrast microscope,histological examination,scanning e- lectron microscope and transmission electron microscope.Results By phase contrast microscope,SCs grew well on the edge of SIS after 3 and 5 days.SCs adhered tightly on the surface of SIS after 5 days through histo- logical examination.By scanning electron microscope,on the surface of SIS,SCs grew and adhered actively, prominence of cells body were obvious.They connected end-to-end with each other or arranged in clusters. Protein granules were secreted on cells surface.By transmission electron microscope,SCs grew in good condi- tion and adhered tightly on the surface of SIS.At the interface of SCs and SIS,prominence was seen to contact with SIS in the bottom of cell body.Conclusion SCs are able to adhere and grow well on the surface of SIS.As a scaffold,SIS has good biocompatibility with SCs.